3.Therapeutic effects of focused ultrasound on chronic cervicitis and the influencing factors
Chinese Journal of Obstetrics and Gynecology 2001;0(01):-
Objective To explore the therapeutic effect of focused ultrasound(FU)on chronic cervicitis unitarily and its influential factors.Methods Three hundred and thirteen patients of chronic cervicitis were treated with the device of CZF model focused ultrasound.The therapeutic effects in 300 cases with complete records,as well as the relationship between the diameter of cervical erosion and the exposure time to FU were analyzed.Results The effective rates of FU in treatment of chronic cervicitis unitarily were 100.0%(53/53),95.2%(120/126)and 91.7%(111/121),respectively,for mild,moderate and severe cases.The overall effective rate was 94.7%(284/300).With other parameters of FU unchanged,the exposure time to FU showed an exponential relationship with the diameter of erosion,and the regression equation was D=31.71e~(0.4808t)(R~2=0.9966).Conclusions The controllability and achievement ratio of FU to treat cervical erosion could be enhanced when exposure time and manners were fully considered based on the diameter and type of cervical erosion.A satisfactory therapeutic efficacy could be achieved with FU to treat cervical erosion unitarily.
4.Advances in Solid-state Fermentation of Microbial Lipase
Xiao-Feng WANG ; Yun-Jun YAN ;
China Biotechnology 2006;0(01):-
Lipases have catalytic active in both aqueous phase and the non-aqueous phase and have a wide range of application in various industrial areas.However,the high cost of lipase production has restricted its extensive use in industry.Solid state fermentation possesses many advantages,such as low requirement for devices,low energy consumption,low production cost,little pollution to environment and easily being popularized,which have made it an important means in microbial production of lipases.Owing to the rapidly increased energy cost and the people's awareness of environmental protection,the solid state fermentation technique,which was regarded as low-tech in the past,has regained attention and developed rapidly since the 1990s.The production of lipase by SSF technique was reviewed.Mainly contents describe its characteristics,including physical and chemical factors and bioreactors.
5.Effect of PYK_2 gene siRNA on proliferation of adult rat cardiac fibroblasts
Jia HAO ; Jun YAN ; Yingbin XIAO
Journal of Third Military Medical University 2003;0(23):-
Objective To design and construct the vector of protein-rich tyrosine kinase 2 small RNA interference and investigate the effect of the recombinant plasmid on the proliferation of adult rat cardiac fibroblasts. Methods The pAVU6+27-PYK_2 siRNA expression vector was constructed by gene recombination, then transfected into the cultured adult rat cardiac fibroblasts by DOTAP method. PYK_2 mRNA and protein in cardiac fibroblasts were determined by RT-PCR and Western blotting respectively. Cell proliferation was tested by MTT and ~3H-TdR incorporation. Results The pAVU6+27-PYK_2 siRNA expression vector was successfully constructed. The recombinant plasmid can inhibit the proliferation of adult rat cardiac fibroblasts, as compared with control. Conclusion pAVU6+27-PYK_2 siRNA expression vector is efficient to suppress DNA synthesis of cardiac fibroblasts, which will be beneficial to further study of myocardial fibrosis.
6.Change of Serum Neuron Specific Enolase in Neonates with Asphyxia before and after Head Mild Hypothermia Treatment
jun, CHEN ; yan, GUO ; xiao-li, SUN
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To explore the change of serum level of neuron specific enolase (NSE) in neonates with asphyxia before and after head mild hypothermia.Methods Eighty-two asphyxial neonates were selected,including 39 mild asphyxial neonates and 43 severe asphy-xial neonates,and 29 healthy neonates were selected as control group.Forty-three severe asphyxial neonates were randomly assigned into mild hypothermia treatment group and traditional treatment group.Neonates in traditional treatment group were just given traditional treatment.While neonates in mild hypothermia treatment group received head mild hypothermia therapy and their nasopharyngeal temperature were maintained at(34.0 ? 0.5) ℃ for 72 h.Before treatment and 72 h after treatment,2 mL blood was collected,and the serum NSE was determined by radio immunoassay.Results NSE levels in mild asphyxial neonates group[(34.83?6.17) ?g/L] and severe asphyxial group[(59.58?8.87) ?g/L] were significantly higher than that of control group[(30.57?4.88) ?g/L](t=3.07 P0.05).The level of NSE at 72 h in severe asphyxial neonates with head mild hypothermia therapy[(40.97?6.55) ?g/L] was significantly lower than that of traditional treatment group [(48.15?5.57) ?g/L](t=3.86 P
7.Phacoemulsification with intraocular lens implantation for high myopia cataract
Xiao-Juan, CHEN ; Yan-Yan, LI ; Jun, CHEN
International Eye Science 2014;(6):1136-1137
AIM: To observe the clinical effect of phacoemulsification combined with intraocular lens implantation for high myopia ( axis oculi ≥ 30mm ) cataract, and to explore the causes and treatment methods of operation complications.
METHODS: Selected 64 cases ( 86 eyes ) of cataract with high myopia patients (axis oculi ≥30mm), vision correction≤ 0. 1. Phacoemulsification combined with intraocular lens implantation was used. During 1a follow-up, the improvement of visual acuity and complications were observed.
RESULTS: Postoperative best corrected visual acuity was ≥0. 4 in 35 eyes (41%), 0. 2-0. 3 in 23 eyes (27%), 0. 1-0. 15 in 25 eyes (29%), <0. 1 in 3 eyes (3%). Posterior capsular rupture, vitreous prolapsed occurred in 2 eyes ( 2%) . Through the anterior vitrectomy, intraocular lens was implanted to ciliary sulcus. Postoperative corneal edema in 6 eyes (7%), which was subsided after treatment; Posterior capsular opacity in 9 eyes (10%), visual acuity was recovered after incision of posterior capsule by YAG laser. Retinal detachment in 1 eyes (1%), which was cured after sclera buckling.
CONCLUSION: Phacoemulsification combined with intraocular lens implantation is a safe and ideal operation method for high myopia cataract, can obtain good effects, and the postoperative complications can be controlled. Because of the particularity of high myopia, operation should be gentle, technical parameters should be adjusted as appropriate, to achieve the best postoperative effects.
8.Comparison of two surgical treatments for pediatric lower eyelid trichiasis
Yan-Xia, XIAO ; Jun-Jie, YAN ; Dong-Qiang, XU
International Eye Science 2014;(6):1171-1173
AIM: To compare the clinical results of suture method and partial eyelash resection treating for pediatric eyelid trichiasis, and screen an effective method for the treatment of pediatric lower eyelid trichiasis.
METHODS: Fifty-six cases of pediatric patients with lower eyelid trichiasis were randomly divided into a control group and an observation group in accordance with the method of drawing lots, and each group was 28 cases. The control group was treated with suture method, and the observation group was treated with partial eyelash resection. The clinical efficacy, patient satisfaction before and after treatment, and the incidence of complications were compared.
RESULTS:(1) The clinically total effective rate was 74%of the control group, which was 89% of the observation group, and there were statistical differences of the clinical efficacy between the two groups ( P<0. 05 ); ( 2 ) the satisfaction scores were (3. 89±1. 22) points and (6. 27±1-86) points of the the control group before and after treatment, which were ( 4. 00±1. 34 ) points and ( 8-95±2-34) points of the observation group, and there were statistical differences of the two groups before and after treatment (P<0. 05), and the scores of the observation group were higher than that of the control group ( P<0-05);(3) complications such as swelling and stitches off appeared in both groups, and the rate was 21% in the control group, which was 11% in the observation group, and there were statistical differences between the two groups (P<0. 05).
CONCLUSION: The treatment of children with lower eyelid trichiasis, suture method is simple and can be performed under local anesthesia in collaboration with children, but with a higher relapse rate, some patients required reoperation;partial resection of eyelashes can be more thoroughly solve the problem of pediatric eyelid trichiasis with low recurrence rate, but children need to be under general anesthesia with some of big risk. So partial resection of eyelashes is unsuitable for using in clinical practice widely and can be used in special cases.
10.Changes of physical activity status of residents in Beijing Yayuncun community
Wanxiang XIAO ; Feng XIAO ; Yan LI ; Yanlan XIAO ; Yi SONG ; Ruili LI ; Jun MA ; Bowen CHEN
Chinese Journal of General Practitioners 2010;09(7):457-460
Objective To evaluate the changes of physical activity status of residents in Beijing Yayuncun(Asian Games Village)Community.Methods A total of 1765 permanent residents over 15 from 600 families living in Yayuncun Communities since 2000 were selected in this prospective study.Thebaseline survey was carried out with the Questionnaire for Residents of Beijing Typical Community in 2004 and 2005.From 2006 to 2008,same questionnaire was used for the follow-up survey,and 1412 residents were responded to the questionnaire.Results The knowledge levels related to physical exercise of Yayuncun residents elevated(P<0.01).The source of knowledge gain from Olympics Games promotion,specialized community lectures and intemet increase significantly(P<0.01),that from other media decreased(P<0.01).The exercise rate in community was higher than in other sites(P<0.01)and increased significantly (P<0.05);while doing exercise in charged sites decreased(P<0.05).Logistic regression analysis showed that regular physical exercise was related to age,BMI,exercise-related knowledge,exercise sites,attitude for sport facilities and chronological order.Conclusion During the preparation for the Beijing Olympic Games.the status of physical exercise of residents in Yayuncun Community presented the positive trends.