1.Causes and treatment of childhood vulvovaginitis
jie, ZHOU ; jing, SUN ; wang-lei, QU ; xiao-jian, CHEN
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To find out and clarify the causes and the pathogens of vulvovagmitis in childhood girls, to determine the clinical treatment. Methods There were 345 cases of childhood girls .ranged from 18 days to 12 years, with symptoms of vulvovagini tis, and their vaginal secretions were examined by routine smear for cleaning degree, trichomonas , Candida and clue cells, gram-stain for neisseria gonorrhoeae, culture for bacteria, mycoplasma urealytium, mycoplasma hominis and chlamydia trachomatis. Results One hundred and nine pathogens were detected(31.6% of the whole cases ), including specific pathogen 70 cases(20. 3% )and non-specif ic pathogen 32 cases(9.3%). Gardnerella was detected most frequently in specific pathogen while enteric bacilli was detected most frequently in non-specific pathogen. Conclusions Non-specific vulvovaginitis is the most frequent cause in childhood vulvovagimtis, and bacterial vaginosis is the mast frequent in specific infection. Symptomatic treatment is effective, but antibiotic treatment should be based on pathogenic findings of vaginal secretions.
2.Effects of glycyrrhizic acid beverage on exercise performance of fe- male undergraduates with moderate endurance training and its mechanism.
Jing QU ; Hong-fang YANG ; Xiao-hui WANG
Chinese Journal of Applied Physiology 2016;32(1):31-33
Beverages
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Exercise
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Female
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Glycyrrhizic Acid
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pharmacology
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Humans
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Physical Endurance
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Students
3.Value of high frequency ultrasound in diagnosis of duodenal obstruction in neonates
Nina QU ; Jie LI ; Dandan SHI ; Jing XIAO ; Meng WANG ; Kaining ZHANG
Chinese Journal of Ultrasonography 2011;20(6):502-504
Objective To evaluate the clinical significance of high frequency ultrasound in the diagnosis of duodenal obstruction in neonates.Methods Ultrasonography,clinical data and etiological diagnoses of the operation in 113 neonates with duodenal obstruction were reviewed retrospectively.The digestive tract,including stomach,duodenum,jejunoileum and colon,were examined in all patients with 8-12 MHz linear transducer before operation.Results In the 113 neonates with duodenal obstruction,63 cases were diagnosed intestinal malrotation,31 cases duodenal stenosis,14 cases annular pancreas,and 5 cases duodenal atresia.One hundred and six cases were diagnosed as duodenal obstruction by ultrasound,of which intestinal malrotation in 61 cases,duodenal stenosis in 29 cases,duodenal atresia in 4 cases,and annular pancreas in 12 cases.The diagnostic rate was 93.81% (106/113 cases),96.83% (61/63 cases),93.55% (29/31 cases),80.00% (4/5cases) and 85.71% (12/14 cases),respectively.The location of obstruction diagnosed by ultrasound was coincident with the operation in 92 cases,with a diagnostic rate of 81.42%(92/113).Conclusions High frequency ultrasound plays an important role in diagnosing the causes and location of duodenal obstruction.It can be used as the first choice of examinal methods for the neonates with duodenal obstruction.
4.Morphogenetic study of human adenovirus type 41 in 293TE cells.
Jing-Dong SONG ; Min WANG ; Xiao-Hui ZOU ; Jian-Guo QU ; Zhuo-Zhuang LU ; Tao HONG
Chinese Journal of Virology 2014;30(2):154-161
To investigate the morphogenetic process of human adenovirus type 41 (HAdV-41), 293TE cells were infected with purified wild-type HAdV-41, and ultrathin sections of infected cells were prepared and observed under a transmission electron microscope. Results showed that HAdV-41 entered host cells mainly through three ways: non-clathrin-coated pit, clathrin-coated pit, and direct penetration of plasma membrane. In addition, cell microvilli might help HAdV-41 enter cells. After entering into cells, HAdV-41 virus particles could be found in vacuoles or lysosomes or be in a free state in cytoplasm. Only free virus particles could be found near nuclear pores (NP), suggesting that the virus needed to escape from lysosomes for effective infection and viral nucleoprotein entered the nucleus through NP. Progeny viruses were as-sembled in the nucleus. Three types of inclusion bodies, which were termed as fibrillous inclusion body, condense inclusion body, and stripped condense inclusion body, were involved in HAdV-41 morphogenesis. In the late phase of viral replication, the membrane integrity of the infected cells was lost and viral particles were released extracellularly. This study reveals the partial process of HAdV-41 morphogenesis and provides more biological information on HAdV-41.
Adenovirus Infections, Human
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virology
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Adenoviruses, Human
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genetics
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growth & development
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physiology
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ultrastructure
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Cell Membrane
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virology
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Cell Nucleus
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virology
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Humans
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Virus Release
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Virus Replication
5.Study of dehydroepiandrosterone retarding atherosclerosis of high cholesterol-fed rabbits.
Heng-hui CHENG ; Zhi-ling QU ; Ying ZHOU ; Zhen-ying BAN ; Xiao-jing HU ; Qiu-rong RUAN
Chinese Journal of Pathology 2007;36(4):263-264
Animals
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Aorta
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pathology
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Atherosclerosis
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blood
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etiology
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metabolism
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Chemokine CCL2
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metabolism
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Cholesterol
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blood
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Cholesterol, Dietary
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administration & dosage
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Cholesterol, HDL
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blood
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Cholesterol, LDL
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blood
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Dehydroepiandrosterone
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pharmacology
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Diet, Atherogenic
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Immunohistochemistry
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Rabbits
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Random Allocation
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Triglycerides
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blood
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Vascular Cell Adhesion Molecule-1
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metabolism
6.Suppression of OCT2/MRP2 decreases kidney injury and enhances the chemosensitivity of co-administration of cisplatin and astragaloside IV
Xiao-yu QU ; Jing-hui ZHAI ; Huan GAO ; Li-na TAO ; Yue-ming ZHANG ; Jia-wei GONG ; Yan-qing SONG
Acta Pharmaceutica Sinica 2021;56(9):2536-2543
Kidney injury and decreased chemosensitivity of tumor cells are obstacles with cisplatin (CDDP) chemotherapy. Down-regulation of the organic cation transporter 2 (OCT2) and multidrug resistance-associated protein 2 (MRP2) is a key means to alleviate CDDP-induced kidney injury and increase chemosensitivity. Astragaloside IV (AS IV) is obtained from the well-known traditional Chinese herb
7.Relationship between genetic polymorphism of VEGF and risk factor to lung cancer
Jing LIANG ; Xiao-Lin LIU ; Dian-Shui SUN ; Hai-Rong LIU ; Wei HU ; Ai-Zhong QU ; Yan LI ;
China Oncology 2006;0(11):-
Background and purpose:Vascular endothelial growth factor(VEGF) is a potent angiogenic mediator and angiogenesis has important effects on tumor growth and metastasis.The present study was to investigate the relationship between genetic polymorphism of VEGF and heredity risk factor of lung cancer.Methods:VEGF genotypes were determined by PCR-RFLP method in 171 patients with lung cancer and 172 healthy controls.Software PHASE 1.0 was used to construct the haplotypes of every individual.Unconditional logistic regression model was used to analyze the statistical association of genotypes or haplotypes in the two groups adjusted by gender and age. Results:Individuals with at least one-2578A allele had a significantly decreased risk of lung cancer compared with those carrying-2578CC genotype.When the analyses were stratified by gender,the combined-2578 CA and AA genotype,were associated with a considerably reduced risk of lung cancer(P=0.001,OR=0.303,95%CI=0.15 3-0.601).The distribution of the two haplotypes(936C/-2578C and 936C/-2578A) among overall lung cancer cases was significantly different from that among the controls(P=0.016,0R=0.317,95%CI=0.124-0.809 and P=0.018,OR=0.547, 95%CI=0.331-0.903).When the cases were categorized by tumor histology,the distribution of C-C haplotype in the adenocarcinoma(AC) group was associated with a substantially lowered risk of AC(P=0.004,0R=0.237,95%CI=0.090- 0.627),compared with the reference haplotypes.Conclusion:VEGF polymorphism may be a critical risk for the genetic risk factor to lung cancer.
8.Acute kidney injury in critically ill children infected with influenza A virus (H1N1) and enterovirus 71.
Jian-guo LI ; Dong QU ; Ying LI ; Fei WANG ; Lin-ying GUO ; Jing-jing WANG ; Li CAO ; Xiao-xu REN
Chinese Journal of Pediatrics 2011;49(11):839-842
OBJECTIVETo analyze the clinical characteristics of acute kidney injury (AKI) in critically ill childhood patients with influenza A virus (H1N1) and enterovirus 71 (EV71), and to study the significance of the serum creatinine and urine output in diagnosis of AKI.
METHODThe clinical data of AKI in critically ill children admitted to intensive care units (ICUs) with confirmed influenza A (H1N1) or enterovirus 71 infection (EV71 group) from Oct. 2009 to Oct. 2010.
RESULTTwenty-eight critically ill children were involved in the study. In H1N1 group, there were 18 cases including 6 males and 12 females, and the average age was 5.4 years. In EV71 group, there were 10 cases including 8 males and 2 females, and the average age was 1.1 years. In H1N1 group: 4 cases developed AKI, whose average number of involved organ was 5.3. Two children were classified as first stage completely recovered after treatment; three children who were classified as third stage died. In 14 children without AKI, the average number of involved organ was 3.0, four of these children died. In EV71 group: 3 cases (first stage) developed AKI and 3 cases' serum creatinine increased to 45.0 to 47.6 percent from baseline. The average number of involved organ was 5.7. All the six children died. The other 4 cases whose serum creatinine was normal, and the average number of involved organ was 3.0, recovered.
CONCLUSIONIn critically ill virus-infected children, more organs were involved in the patients who developed AKI. As to influenza A (H1N1) infected critically ill children, the prognosis was comparatively better if the children were classified as AKI stage 1 and received early effective treatment. On the contrary, the prognosis was comparatively worse for those with AKI stage 3. As to EV71 infected critically ill children, the prognosis was worse once AKI developed. As to diagnosis of AKI, the sensitivity of serum creatinine criteria seemed to be superior to the urine output criteria. However, the significance of the serum creatinine and urine output in diagnosis of AKI still needs to be investigated in the future in large scale clinical studies.
Acute Kidney Injury ; diagnosis ; etiology ; virology ; Child ; Child, Preschool ; Critical Illness ; Enterovirus ; pathogenicity ; Enterovirus Infections ; complications ; virology ; Female ; Humans ; Infant ; Influenza A Virus, H1N1 Subtype ; pathogenicity ; Influenza, Human ; complications ; virology ; Intensive Care Units ; Male ; Prognosis ; Retrospective Studies
9.Expression of human tumstatin in Pichia pastoris and its bioactivity.
Qu-Liang GU ; Tian-Yuan ZHANG ; Jin-Xian LUO ; Jing-Jing GAN ; Fan XIAO
Chinese Journal of Biotechnology 2006;22(3):451-456
Human tumstatin(hTumstatin)cDNA was amplified from recombinant plasmid pET-3c-tum, cloned in frame with the signal sequence in yeast vector pPICZalphaA and transformed into Pichia pastoris GS115 by electroporation. The expression of hTumstatin in GS115(pPICZalpha-tum)was then induced by methanol and secreted into the culture medium, with a yield of 25mg/L as shown by SDS-PAGE and Western blotting. The expressed hTumstatin was purified to more than 85% purity using a simple one-step SP-Sepharose cation exchange chromatography. The MTT and chick chorioallantoic membrane assay showed that the yeast produced hTumstatin could inhibit the proliferation of human umbilical vein endothelial cells and the neovascularization induced by bFGF. Hoechst 33258 fluorescent staining also demonstrated the apoptotic change in endothelial cellular nuclear morphology.
Angiogenesis Inhibitors
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metabolism
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Autoantigens
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genetics
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metabolism
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Cell Proliferation
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Cells, Cultured
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Collagen Type IV
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genetics
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metabolism
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DNA, Complementary
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genetics
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Electroporation
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Endothelial Cells
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cytology
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Humans
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Pichia
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genetics
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metabolism
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Recombinant Proteins
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genetics
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metabolism
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Umbilical Cord
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cytology
10.A pilot study on spinal muscular atrophy carrier screening in Shanghai region using real-time PCR.
Xiao-xing QU ; Bing XIAO ; Xing JI ; Wen-ting JIANG ; Zu-jing YANG ; Jiong TAO
Chinese Journal of Medical Genetics 2013;30(1):1-4
OBJECTIVETo develop a screening program for spinal muscular atrophy (SMA) carriers, and to assess the carrier frequency and detection rate in Shanghai region.
METHODSQuantitative analysis of the SMN1 gene by real-time PCR was developed using specimens from 15 SMA patients and 76 SMA parents from 38 affected nuclear families. A pilot screening was carried out for 1741 asymptomatic pregnant women. Frequencies of SMN1 alleles were determined with the Hardy-Weinberg equilibrium.
RESULTSForty five out of the 1741 women were identified as SMA carriers by the presence of single copy of SMN1. The frequencies of no copy, 1 copy, 2 copy and 3 copy alleles were 1.37 U+00D7 10-2, 9.45 U+00D7 10-1, 2.80 U+00D7 10-2 and 1.27 U+00D7 10-2, respectively. The adjusted SMA carrier frequency was 1:35 with a detection rate of 94.49%. For those with a negative screening result, individuals with 3 copies carried a higher residual risk.
CONCLUSIONThe incidence of SMA carriers in Shanghai region is similar with that in Caucasian populations. Carrier screening has high detection efficiency. An effort should be made to further distinguish SMN1 gene copy numbers for those with more than 2 copies, since accurate determination of 2 and 3 copy allele frequencies is essential for post-screening genetic consulting.
Alleles ; Female ; Gene Dosage ; Gene Frequency ; Heterozygote ; Humans ; Male ; Muscular Atrophy, Spinal ; diagnosis ; genetics ; Pilot Projects ; Pregnancy ; Real-Time Polymerase Chain Reaction ; Survival of Motor Neuron 1 Protein ; genetics