1.One case report of Patau syndrome.
Chinese Journal of Pediatrics 2011;49(7):555-556
3.The Effects of Ouabain on the Inner Ear Glial Cells in Mouse
Zhijian ZHANG ; Hongxia GUAN ; Kun YANG ; Bokui XIAO ; Hua LIAO ; Yang JIANG ; Tao ZHOU ; Qingquan HUA
Journal of Audiology and Speech Pathology 2017;25(5):502-505
To study the effects of ouabain on the inner ear glial cells, and to lay the foundation for the study of stem cell transplantation in the treatment of sensorineural hearing loss.Methods Sixty adult female SPF grade CBA / J mice were randomly divided into experimental group and control group, with 30 mice in each group.Animals in the experimental group received 3mM ouabain via the round window membrane, while mice in control group received normal saline.The mice were sacrificed at 7 days, 14 days and 30 days after the administration,respectively.Immunofluorescence histochemical staining was used to detect the inner ear glial cells in spiral ganglion.Results Some inner ear glial cells survived in the spiral ganglion of the experimental group, while with decreased numbers and disorganized structure compared to those of in the control group.Comparing to those of in the control group, the number and density of inner ear glial cells in the experimental group were significantly decreased from 7 days afterouabain administration,further decreased at 14 days and reduced to the lowest at 30 days after ouabain administration, the differences between the 2 groups were statistically significant (P < 0.05).Among the experimental group, the number of inner ear glial cells at 30 days was significantly decreased when compared to those of at 7 days and 14 days, respectively.Conclusion Application of ouabain to mouse inner ear via the round window membrane leads to an acute and progressive direct damage to the inner ear glial cells in the spiral ganglion.
4.A few questions on the major collateral of stomach.
Yu-Shan FAN ; Xiao-Che FENG ; Tao LIANG ; Chang-Hua TANG ; Fu-Rui MIAO
Chinese Acupuncture & Moxibustion 2014;34(3):300-302
It is held by some of the researches that the "16 collaterals" is composed of the "15 collaterals" and "the major collateral of stomach". And it is included into the textbook that Xuli, the major collateral of stomach, is the pulsation point at the cardiac region. Xuli is often explained as the empty portion of the human body by many researches. Through analysis and summarization of the related theory of the major collateral of stomach, the above mentioned opinion is discussed. And the understanding on the major collateral of stomach is deepened. As a result, it is concluded that count the major collateral of stomach into the 16 collaterals together with the 15 collaterals is inadvisable. The real pulsation point at the cardiac region locates under the left breast. And the real meaning of Xuli is "extending in all directions".
Acupuncture
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history
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China
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Collateral Circulation
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History, Ancient
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Humans
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Medicine in Literature
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Meridians
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Stomach
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blood supply
5.Effects of intracellular calcium alteration on SK currents in atrial cardiomyocytes from patients with atrial fibrillation.
Hua WANG ; Tao LI ; Li ZHANG ; Yan YANG ; Xiao-Rong ZENG
Chinese Journal of Applied Physiology 2014;30(4):296-305
OBJECTIVESK channels are existed in hearts of mouse, rat, and human. Biochemical evidence indicates that SK2 channels are expressed more in atrial than in ventricular tissue. SK channels are highly sensitive to the calcium concentration of the pipette solution. In the present study, performed whole-cell patch clamp was used to detect the calcium sensitivity of small conductance Ca(2+)-activated K+ channels (SK) currents between sinus ryhthm (SR) and auricular fibrillation (AF).
METHODSThe patients who accepted cardiopulmonary bypass were divided into two groups: 21 patients with SR and 8 patients with AF. The enzymatic dissociation method was improved according to the previous research by our lab. The performed whole cell patch-clamp technique was used to record SK2 currents in both SR and AF groups at room temperature.
RESULTSThe SK2 current density was (-2.92 +/- 0.35) pA/pF in SR group (n = 6) vs (-6.83 +/- 0.19) pA/pF in AF group at -130 mV (n = 3, P < 0.05). In SR group, the SK2 current densities in calcium concentration of the pipette solution are (-1.43 +/- 0.33) pA/pF (n = 7), (-2.92 +/- 0.35) pA/pF (n = 6), (-10.11 +/- 2.15) pA/pF (n = 8, P < 0.05); In AF group, the SK2 current densities are (-2.17 +/- 0.40) pA/pF (n = 4), (-6.83 +/- 0.19) pA/pF (n = 3), (-14.47 +/- 2.89 pA/pF) (n = 4, P < 0.05).
CONCLUSIONThe SK2 currents recorded in this experiment are voltage-independent, inwardly rectifying and apamin-sensitive. When the calcium concentration of the pipette solution is 5 x 10(-7) mol/L, SK2 current density in AF group are significantly larger than those in SR group. It suggests that SK currents involve the cardiomyocytes electric remodeling in AF. In AF group, the SK2 currents are more sensitive to free calcium ion. It shows that the increased sensitivity of SK2 currents to the calcium contribute to the occurrence and maintenance of AF.
Atrial Fibrillation ; metabolism ; physiopathology ; Calcium ; metabolism ; Cells, Cultured ; Heart Atria ; metabolism ; Humans ; Membrane Potentials ; physiology ; Myocytes, Cardiac ; metabolism ; Patch-Clamp Techniques ; Potassium Channels, Calcium-Activated ; physiology
6.Emphysema associated with acquired cutis laxa.
Xue-Feng SUN ; Yue-Hua LIU ; Yi XIAO ; Tao LIU ; Xu ZHONG
Chinese Medical Journal 2013;126(6):1194-1194
Adult
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Cutis Laxa
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complications
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diagnosis
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Emphysema
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diagnosis
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etiology
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Female
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Humans
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Male
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Middle Aged
7.Application of cytometric bead array in rapid high-throughput detection of small molecules.
Chang-bin XIAO ; Wei-jun KONG ; Qiu-tao LIU ; Mei-hua YANG ; Li WAN
China Journal of Chinese Materia Medica 2015;40(18):3515-3523
Cytometric bead array (CBA) is a new analytical technique, which can achieve real-time and rapid detection of targeted components in a small amount of sample. With many advantages of high throughput screening, high specificity and sensitivity, low cost, easy operation and good repeatability, this CBA technique has been widely used for the detection of various components in foods, agricultural products and environmental samples. Recently, it has got significant development in rapid detection of small molecules. This review briefly introduced the theory of CBA technique, summarized the application in the analysis of small molecules, such as mycotoxins, pesticide residues, shellfish toxins, and then prospected the application of trace small molecules detection in the complex matrices of traditional Chinese medicine and the development trend of it.
Drug Contamination
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High-Throughput Screening Assays
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instrumentation
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methods
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Immunoassay
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instrumentation
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methods
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Microspheres
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Pesticides
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analysis
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Toxins, Biological
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analysis
8.Purification of recombinant human IE—2 by McAb affinity chromatography
Xiao-Li WANG ; Ben XU ; Lan ZHENG ; Ying-Hua XIE ; Xian-Tao KONG ;
Chinese Journal of Immunology 1985;0(01):-
For purifying recombinant human IL—2 (rhIL—2),the columns of immunoabsorptionwere prepared with 4 anti—IL—2 McAb (9B12,9F5,9B2 and 8H7) purified by caprylic acid.Although 4 McAbs differ as regards their antigen—antibody binding characteristics,all they canserve as effective immnoabsorbents,provided optimum condition was adopted.The recoveryrate of 9B12,9F5,8H7 and 9B2 columns were 49.2%,37.5%,31.5% and 18.8% respec-tively.The purity of rhIL—2 obtained was more than 95% and biological activity remainedhigher.
9.Applied anatomy of upper fibular bone flap pedicled with circumflex fibular neck artery
Zhenguang CHEN ; Xiao-Hui ZHENG ; Yun XIE ; Sheng-Xiang TAO ; Yu-Hua YANG ;
Chinese Journal of Microsurgery 2006;0(05):-
Objective To provide applied anatomy basis for upper fibular bone flap pedieled with cir- cumflex fibular neck artery.Methods The origin,course,branches,distribution and anastomosis of cir- cumflex fibular neck artery were observed on 54 sides of adult cadaver lower limb speeimens perfused with red latex.Results The circumflex fibular neck artery derived from popliteal artery (3.3?1.1)cm beneath the tip level of fibular head,with its length of (1.4?1.0) cm and diameter of (1.4?0.5) mm.The ascending periosteal branches of circumflex fibular neck artery anastomosed consistently with the descending periosteal branches of inferior lateral genicular artery.Conclusion The transplanting operative design of upper fibular bone flap pedicled with circumflex fibular neck artery was feasible.
10.Diagnosis of X-Linked creatine transporter deficiency in a patient from Northeast China
Chun-Hui Hu ; Yu-Ying Fan ; Long-Fei Wang ; Tao Yu ; Xiao-Ming Wang ; Hua Wang
Neurology Asia 2015;20(2):197-201
Background: Creatine transporter (CRTR) deficiency is the most common creatine deficiency syndrome,
of which the final diagnosis relies on mutation in the X-linked CRTR gene. To date, more than 90
mutations in the SLC6A8 gene have been reported. This paper discusses a novel mutation detected via
the thorough sequencing of all the X-chromosome-specific exons investigated in a four and a half year
old boy with an intellectual disability, speech and language delay and motor disturbance. Methods:
A brain magnetic resonance imaging (MRI) and a proton magnetic resonance spectroscopy (MRS)
were carried out, the creatine and creatinine concentrations in the urine were checked and all exons
were sequenced. Results: A detailed clinical investigation revealed a reduction in the cerebral creatine
levels in the brain by the MRS, elevated creatine and creatinine concentrations in the urine and signal
abnormalities in the left frontal cortex of the brain by the MRI. A novel change was identified in the
heterozygosity of the exon 10: c.1395-c.1401 deletion.
Conclusion: The use of a combination of powerful new technologies, such as thorough exome-nextgeneration
sequencing and a brain MRS, should be considered, in order to determine any neurometabolic
diseases, especially when the signal abnormalities in the brain MRI cannot be explained by any other
factors. This mutation results most likely in a dysfunction of the creatine transport and synthesis,
hence causing central nervous system symptoms.
Carrier Proteins