1.Relationship between retinol binding protein-4 and metabolic syndrome
Lingling XU ; Xinhua XIAO ; Qi SUN ; Heng WANG
Basic & Clinical Medicine 2006;0(07):-
Objective To investigate the relation between retinol binding protein-4(RBP4) levels and metabolic parameters and to explore the correlation between RBP4-G 803 A SNP and metabolic syndrome.Methods One hundred and sixteen metabolic syndrome(MS) patients with type 2 diabetes mellitus and 93 healthy controls were included.Serum RBP4 level was measured by RIA.RBP4-G 803 A different genotypes were examined by PCR and sequence analysis.Results RBP4 levels showed significant positive correlations with BMI,waist circumference,FINS,HOMA-IR,and TG in all the participants,with TG,TC,and LDL-C in control,with DBP in males,and with age,FINS,HOMA-IR,TG and SBP in females.In multiple stepwise regression analysis,RBP4 was independently associated with BMI,waist circumference,TG and age.Serum RBP4 concentrations were significantly higher in MS group than in control.RBP4 levels were higher in males as compared to females.In control,RBP4 level was lower in subjects with regular exercise.There was no significant difference in the frequency of RBP4-G 803 A genotypes and alleles in MS group and neither difference in metabolic parameters between the carriers of GG+GA and AA.Conclusion In Chinese Han population,serum RBP4 concentrations are correlated with various metabolic parameters and there is no relation between RBP4-G 803 A SNP and metabolic syndrome.
2.An anatomical study and clinical application of free transfer of foot tissues pedicled with plantar metatarsal artery
Xiao-Chao CHU ; Cheng YANG ; Yong-Jun XU ; Qingsheng ZHU ; Rui CONG ; Hongyin DU ; Heng LI ;
Chinese Journal of Orthopaedic Trauma 2004;0(10):-
Objective To explore a new method of functional reconstruction of hand digits and joints with free transfer of foot tissues so as to increase the success rate of the operation.Methods After micro-anatomic study of the plantar and dorsal metatarsal arteries,retrograde and free grafts of foot tissues pedicled with plantar metatarsal arteries were designed and applied in transplantation to treat 76 cases of hand digital or joint defects.The surgeries included 58 cases of transfer of the second toe,four cases of transfer of composite tissues of the second toe, eight cases of transfer of proximal interphalangeal joint,and six cases of nail flap transfer.Results The mi- cro-anatomic study found that the first plantar metatarsal artery was anatomically constant and the diameter of its branch to the second toe was larger than that of the first dorsal metatarsal artery.Flaps survived in 75 of the 76 patients(98.7%),with fine appearance and significantly improved function.One patient who had received free transfer of the second toe to reconstruct the thumb function had to undergo a second repair with infraclavicula skin tube because of refractory arteriospasm of anastomosed vessels.Conclusion Transfer with free retrograde grafts of foot tissues pedicled with plantar metatarsal artery to reconstruct hand functions can effectively improve the success rate of the operation,because it is free of the shortcomings of great anatomic variation of blood vessels and time-consuming and complex procedures in conventional transfer.
3.Effects of electroacupuncture on IGF-1 in the ischemic cortex after cerebral ischemia
Na XIAO ; Heng XIANG ; Liping HUANG ; Shi ZHOU ; Jianqi ZHANG ; Naxing XU
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(1):16-20
Objective To investigate the effects of electroacupuncture (EA) on affected and healthy limbs and on the regulation of insulin-like growth factor-1 (IGF-1) mRNA and proteins in the cerebral cortex early after focal ischemic.Methods A model of focal cerebral ischemia was established in 54 SD rats using the suture occlusion method.They were then randomly divided into an affected limb therapy group (ALTG,n =18),an unaffected limb therapy group (UALTG,n =18),and a control group (CG,n =18).Each group had a 7-day subgroup,a 14-day subgroup and a 21-day subgroup with 6 rats in each.Rats in the experimental groups received EA beginning 24h after the occlusion.Rats in each subgroup were sacrificed in a random order on the 7th,14th and 21st days and the ischemic cerebral cortexes were quickly dissected.The specimens were frozen in liquid nitrogen before being analysed for IGF-1 mRNA expression by RT-PCR and for IGF-1 protein by Western blotting.Results ①After occlusion,IGF-1 protein levels in the ischemic cortexes of the CG declined from the 7th through the 21st day.Rats in the ALTG had significantly higher levels compared with the CG at all time points.The UALTG had the highest values on the 14th day,but was lower than the ALTG and higher than the CG at the 21st day.②IGF-1 mRNA levels in the ischemic cortexes of the UALTG declined from the 7th through the 21st day.At day 7 the results of the UALTG were 6.8 times higher than the CG,and the ALTG was 3.0 times higher.At day 14 levels in the UALTG were significantly lower than those in the ALTG.At that point the results of the UALTG rats were 3.3 times higher than those of the CG and the ALTG was 5.7 times higher.On day 21 levels in both the UALTG and ALTG were significantly lower than in the CG.Conclusions EA intervention at an early stage of focal cerebral ischemia can improve the expression of IGF-1 mRNA and protein levels in the ischemic cortex.Treating the unaffected limb can evoke more IGF-1 mRNA expression earlier and with relatively longer duration,and generate relatively longer protein increases.EA administered to the unaffected limb was more effective in the early stage of stroke.
4.The influence of CTP-OD1-HA and CTP-OD2-HA fusion peptides or combine with imatinib on proliferation of K562 cells
Heng XIAO ; Yanbin REN ; Zhiming YANG ; Shujie ZHOU ; Lei YIN ; Zhimei QIN ; Ling XU ; Shouxia LI
International Journal of Laboratory Medicine 2017;38(14):1876-1878
Objective To study the influence of CTP-OD1-HA and CTP-OD2-HA fusion peptides and combined with imatinib on proliferation of K562 cells.Methods K562 cells were treated with CTP-OD1-HA and CTP-OD2-HA peptides or together with imatinib.The proliferation of cells were detected and compared by MTT and clone formation methods.Results MTT examination demonstrated that CTP-OD1-HA and CTP-OD2-HA peptides could inhibit the proliferation of K562 cells,and the effect was more obvious when acted along with imatinib;Clone formation showed that CTP-OD1-HA and CTP-OD2-HA peptides suppressed the continuous colony forming ability of K562 cells.Conclusion CTP-OD1-HA and CTP-OD2-HA could specially inhibit the proliferation of K562 cells,and increase the sensitivity of imatinib.
5.Secretory carcinoma of breast in male: report of a case.
Yan XU ; Qun-Li SHI ; Xiao-Jun ZHOU ; Heng-Hui MA ; Hang-Bo ZHOU
Chinese Journal of Pathology 2009;38(10):707-708
Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Breast Neoplasms, Male
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metabolism
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pathology
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surgery
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Cadherins
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metabolism
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Carcinoma
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metabolism
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pathology
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surgery
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Diagnosis, Differential
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Follow-Up Studies
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Humans
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Keratin-5
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metabolism
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Lymph Node Excision
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Male
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Mastectomy
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methods
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Mucin-1
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metabolism
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S100 Proteins
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metabolism
6.Topically applied hypericin exhibits skin penetrability on nude mice
LI ZHUO-HENG ; LI YUAN-YUAN ; LU LAI-CHUN ; XU XIAO-YU
Chinese Journal of Pharmacology and Toxicology 2017;31(10):1020-1020
OBJECTIVE Hypericin, a powerful naturally photosensitizer in photodynamic therapy (PDT), is suitable for treating skin diseases involving excess capillary proliferation. In the present study, we aimed to evaluate the skin penetrability of a topically applied hypericin, expecting reducing the risk of prolonged skin photosensitivity, which often occurs after systemic administration. METHODS The Franz diffusion cell assay was performed to evaluate different penetration enhancers. In vivo studies, fluorescence microscopy was performed to examine the distribution of hypericin in the skin, macroscopic and microscopic analyses were also carried out to detect pathological changes in the skin after topical hypericin-PDT treatment. Immunohistochemistry was used to determine the expression of PECAM-1 in the treated skin. RESULTS 5% menthol facilitated hypericin penetrate the skin of nude mice most. The results of in vivo assays revealed that hypericin penetrated nude mice skin, spread to the dermis, and resulted in obvious photosensitivity reaction on the dermal capillaries. Moreover, skin injured by the photosensitive reaction induced by hypericin was replaced by normal skin 7 d after hypericin-PDT treat?ment. CONCLUSION Topical hypericin could penetrate nude mouse skin well and be great potential in PDT treatment of skin diseases.
8.Deep vein thrombosis after closed fracture of lower extremity and blood types ABO
Shuhao LI ; Kun ZHANG ; Zhe SONG ; Wei FAN ; Xiaolong WANG ; Chen WANG ; Dongxu FENG ; Lisong HENG ; Fan XU ; Xiao CAI ; Pengfei WANG ; Yangjun ZHU
Chinese Journal of Orthopaedic Trauma 2021;23(1):81-87
Objective:To investigate the differences in incidence of deep vein thrombosis (DVT) after closed fracture of lower extremity between patients with different blood types ABO.Methods:A retrospective study was conducted in the 1, 951 patients who had been admitted to Department of Orthopaedics Trauma, Honghui Hospital Affiliated to Xi'an Jiaotong University for lower extremity fractures from August 2014 to June 2018. They were 924 males and 1,027 females with a mean age of 63 (46, 78) years (range, from 16 to 102 years). Of them, 572 were type O, 564 type A, 609 type B and 206 type AB. Venous ultrasonography was performed on both lower extremities within 12 hours after admission. The incidences of DVT after fracture were compared between different blood types in all the patients, patients with proximal fracture of the knee, peri-knee fracture and distal fracture of the knee, and patients<60 years old and ≥60 years old.Results:The incidences of DVT were, respectively, 26.75% (153/572), 28.72% (162/564), 34.32% (209/609) and 29.61% (61/206) for patients with blood type O, type A, type B and type AB. The DVT incidence for type B was significantly higher than that for type O ( P< 0.008). The incidences of DVT were, respectively, 28.74% (98/341), 28.99% (100/345), 39.45% (144/365) and 30.97% (35/113) for blood type O, type A, type B and type AB in the patients with proximal fracture of the knee. The DVT incidence for blood type B was significantly higher than those for blood type O and blood type A ( P< 0.008). There were no significant differences in the DVT incidence between different blood types ABO in the patients with peri-knee fracture, distal fracture of the knee,<60 years old or ≥60 years old( P>0.05). The incidences of DVT were, respectively, 30.99% (97/313), 33.33% (108/324), 45.22% (156/345), 34.74% (33/95) for blood type O, type A, type B and type AB in the patients ≥60 years old. The DVT incidence for blood type B was significantly higher than those for blood type O and blood type A ( P< 0.008). Conclusions:The incidence of DVT varied with different blood types ABO after lower extremity fracture. The highest DVT incidence was found in patients with blood type B. The impact of blood type on the DVT incidence after lower extremity fracture was mainly observed in the patients with proximal fracture of the knee or an age of ≥ 60 years old.
9.Antioxidative protective effect of icariin on the FeSO4/H 2O 2-damaged human sperm based on confocal raman micro-spectroscopy.
Zhan-Sen, HUANG ; Heng-Jun, XIAO ; Tao, QI ; Zhi-Ming, HU ; Hao, LI ; Di-Ling, CHEN ; Ya-Lin, XU ; Jun, CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(5):755-60
Oxidative stress is implicated in male infertility and significantly higher reactive oxygen species are detected in 25% of infertile males. Although different agents of various alternative medicines, including traditional Chinese medicine, have been tried with varying success, evidence remains limited on whether and how much herbs or supplements might help increase the anti-oxidant ability of the sperm. This study examined the anti-oxidative effects of icariin, a flavonoid isolated from Herba Epimedii, on the human sperm. We prepared the FeSO4/H2O2-damaged human sperms, which were co-cultured with icariin in vitro, and then observed the changes of the sperm by employing Raman micro-spectroscopy. The results showed that Raman mapping with a 514 nm excitation laser allowed clear differentiation of the nucleus, neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of icariin on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milli-seconds, and icariin could keep the "Raman fingerprint" of the human sperm the same as the control groups, suggesting that icariin could protect the human sperm from being damaged by FeSO4/H2O2. Icariin may serve as a tonifying and replenishing agent of herbal origin for enhancing reproductive functions.
10.Etiology and high risk factors of neonatal ventilator-associated pneumonia.
Xu-Liang ZHU ; Ling ZHAO ; Jia-Chen YANG ; Xiao CHEN ; Xing-Heng WU
Chinese Journal of Contemporary Pediatrics 2007;9(6):549-552
OBJECTIVEVentilator-associated pneumonia (VAP) is a common nosocomial infection and is responsible for a very high mortality in neonatal intensive care unit (NICU) patients. This study was designed to investigate the etiology and high risk factors of neonatal VAP.
METHODSThe clinical data of 106 critical neonates who were treated with mechanical ventilator between 2003 and 2005 were studied retrospectively.
RESULTSOf the 106 neonates, 84 received mechanical ventilation for > or = 48 hrs. Thirty-five (41.7%) out of the 84 patients developed VAP. Univariate analysis showed that gestational age, duration of mechanical ventilation, reintubation, birth weights, primary lung disease and gamma globulin administration were associated with the development of VAP (P < 0.05). Multivariate stepwise logistic regression analysis showed that primary lung disease (OR=3.671, 95% CI=1.0-13.45, P < 0.05), duration of mechanical ventilation (OR=4.945, CI=1.51-16.21, P < 0.01), reintubation (OR=7.721, 95% CI=2.31-25.85, P < 0.01) and high-dose gamma globulin administration (OR=5.520, 95%CI=2.08-16.26, P < 0.01) were predicted factors for the development of VAP. The detection rate of gram negative bacilli (76.9%) was the highest, followed by gram positive coccus (17.9%) in VAP patients.
CONCLUSIONSOpportunistic drug-resistant bacteria are common pathogens for neonatal VAP. The risk of VAP is multifactorial, including external medical environments and patients' internal agents.
Anti-Bacterial Agents ; therapeutic use ; Female ; Humans ; Infant, Newborn ; Logistic Models ; Male ; Microbial Sensitivity Tests ; Pneumonia, Ventilator-Associated ; drug therapy ; etiology ; Retrospective Studies ; Risk Factors