1.Clinical observation on the effect of resection of eyelid tumor and stageⅠ recovery
International Eye Science 2014;(8):1535-1536
AIM: To investigate clinical effects of local resection of eyelid tumors and pathological examination or frozen section examination as well as the stage I recovery.
METHODS: For all 92 cases 92 eyes of eyelid tumor patients who underwent local resection, reconstruction in stage Ⅰ and routine pathological examination ( 37 cases of suspected malignant tumors using frozen section examination. If it was malignant, excision by Moths surgery) . Using free palate mucosa autograft in stage I or reconstruction by Hughes surgery combined with eye flaps repaired eyelid defects.
RESULTS: The postoperative pathological diagnosis:52 cases were benign tumors, and 40 cases malignant tumors. All cases of eye flaps and hard palate mucosa grafts were survived. Followed up for more than 6mo, no tumor were recurred. The appearance and function of eyelid were satisfactory.
CONCLUSION: In this group of cases, nearly half of eyelid tumors are malignant tumors. It should be treated and operated as early as possible. For suspected malignant tumors, we use frozen section examination to confirm its properties in order to excision cleanly in stageⅠ, reducing the recurrence and metastasis. According to the eyelid defect after resection, we could do repair operation in stage Ⅰ. The inner larger defect is repaired by using hard palate mucosa transplantation or Hughes surgery. The outer layer of the eyelid defect is repaired by using eye flap glide, the free flap, the kite flap, the simple suture, etc. It should be careful when using the hard palate mucosa transplantation in the upper eyelid defect.
2.Treatment outcome of allogeneic hematopoietic stem cell transplantation in patients with hematologic malignancies not in remission.
Hai-Yan BAO ; Xiao-Jin WU ; Xiao MA
Chinese Journal of Oncology 2012;34(2):156-157
Adolescent
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Adult
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Disease-Free Survival
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Female
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Graft vs Host Disease
;
etiology
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prevention & control
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Hematologic Neoplasms
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therapy
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Hematopoietic Stem Cell Transplantation
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adverse effects
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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therapy
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Leukemia, Myeloid, Acute
;
therapy
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Lymphocyte Transfusion
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Lymphoma, Non-Hodgkin
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therapy
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Male
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Middle Aged
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Recurrence
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Survival Rate
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Transplantation, Homologous
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Young Adult
3.Hypertrophic cardiomyopathy with right aortic arch, right descending aorta, and Kommerell's diverticulum: a case report.
Xiao-han FAN ; Hai-ying WU ; Si-yong TENG
Chinese Journal of Cardiology 2009;37(8):755-756
Cardiomyopathies
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complications
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Diverticulum
;
complications
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Hematoma
;
complications
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Humans
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Male
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Middle Aged
4.Application of high frequency electrical capsulotomy in maturation period cataract surgery
Wen-Hai, WANG ; Xiao-Yan, WU ; Jian-Lan, ZHANG
International Eye Science 2016;16(10):1927-1928
AIM: To explore the clinical effects of high frequency electrical capsulotomy in maturation period cataract surgery.
●METHODS: A total of 68 cases of maturation period cataract were selected and underwent the surgery of continuous circular capsulorhexis using the high frequency electrical capsulotomy.
●RESULTS: The success rate was 91% in 68 cases with the high frequency electrical capsulotomy.
● CONCLUSION: The high frequency electrical capsulotomy in maturation period cataract surgery has significant advantages and brilliant clinical values.
5.Mid-term follow-ups of hydroxyapatite extensively-coated femoral prosthesis for elderly patients
Yun-Li ZHU ; Hai-Shan WU ; Xiao-Hua LI ;
Chinese Journal of Orthopaedic Trauma 2004;0(08):-
Objective To evaluate the effects of hydroxyapatite (HA) extensively-coated prosthesis on elderly patients undergoing hip arthroplasty.Methods From February 1997 to May 1999,96 patients Were treated with hydroxyapatite (HA) coated prosthesis and followed up for their hip arthroplasty.Their results were studied clinically and roentgengraphically to evaluate chiefly their Harris scores,complications and thigh pain.The survival of prosthesis was evaluated according to Kaplan-Meier methods.Results The 60 cases were followed up for eight years on average(range,sevon to nine years).Their mean Harris scores were improved from 42 preoperatively to 90 postoperatively.Fifty-eihgt cases felt no pain at their hip,but two did at their thigh.No radiolucent lines were observed.All the prostheses achieved bony stabilization and remolding.Conclusions HA coated prosthesis can achieve fine fixation,because it helps bone conduction,resists axial force and torsion,and reduces operative risks.
6.Cloning,High Level Expression and Purification of Human Survivin
Hai LI ; Yu PENG ; Xiao-Tian LI ; Wen-Yan WU ;
China Biotechnology 2006;0(10):-
Survivin is a protein that inhibits apoptosis and regulates cell division.The cDNA sequence of survivin was amplified by RT-PCR and sub-cloned into the prokaryotic expression vector pET-21b(+),followed by transformation into E.coli strain BL21(DE3) and induction with IPTG.The recombinant survivin protein fusing with 6?His tag was expressed in E.coli in the form of inclusion body at the expression level over 60% of the total cell protein.Results of Western blotting showed that recombinant survivin reacted specifically with anti-human survivin antibody.After gel filtration,the recombinant protein reached the purity over 95%,which facilitate the study of diagnosing and inhibitor agents targeting survivin.
7.Meningeal hemangiopericytoma: a clinicopathologic study of 17 cases.
Hai-Bo WU ; Hai-Yan WENG ; Min DING ; Ping GU ; Xiao-Qiu WANG ; Wen ZHONG
Chinese Journal of Pathology 2011;40(4):251-252
Adult
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Aged
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Antigens, CD34
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metabolism
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Chondrosarcoma, Mesenchymal
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metabolism
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pathology
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Hemangiopericytoma
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metabolism
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pathology
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radiotherapy
;
surgery
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Humans
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Male
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Meningeal Neoplasms
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metabolism
;
pathology
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radiotherapy
;
surgery
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Meningioma
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metabolism
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pathology
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Middle Aged
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Neoplasm Recurrence, Local
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Solitary Fibrous Tumors
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metabolism
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pathology
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Vimentin
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metabolism
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Young Adult
8.Gastric adenocarcinoma associated with myelolipoma of parietal pleura: a case report.
Dong-sheng ZHANG ; Dong-hai LI ; Hai-xia BI ; Xiao-feng WU
Chinese Journal of Pathology 2006;35(7):437-437
Adenocarcinoma
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pathology
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surgery
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Biopsy
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Diagnosis, Differential
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Gastric Fundus
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pathology
;
surgery
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Humans
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Male
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Middle Aged
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Myelolipoma
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pathology
;
surgery
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Neoplasms, Multiple Primary
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pathology
;
surgery
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Pleural Neoplasms
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pathology
;
surgery
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Stomach Neoplasms
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pathology
;
surgery
9.Pandanus tectorius derived caffeoylquinic acids inhibit lipid accumulation in HepG2 hepatoma cells through regulation of gene expression involved in lipid metabolism.
Chong-ming WU ; Hong LUAN ; Shuai WANG ; Xiao-po ZHANG ; Hai-tao LIU ; Peng GUO
Acta Pharmaceutica Sinica 2015;50(3):278-283
The fruit of Pandanus tectorius (PTF) has a long history of use as a folk medicine to treat hyperlipidemia in Hainan province, South China. Our previous studies have shown that the n-butanol extract of PTF is rich in caffeoylquinic acids and has an adequate therapeutic effect on dyslipidemic animals induced by high-fat diet. In this work, seven caffeoylquinic acids isolated from PTF were screened for the lipid-lowering activity in HepG2 hepatoma cells. Oil-Red O staining, microscopy and intracellular triglyceride (TG) and total cholesterol (TC) quantification showed that 3-O-caffeoylquinic acid (3-CQA), 3, 5-di-O-caffeoylquinic acid (3,5-CQA), and 3,4,5-tri-O-caffeoylquinic acid (3,4,5-CQA) significantly inhibited lipid accumulation induced by oleic acid and decreased intracellular levels of TC and TG in a dose-dependent manner. These three caffeoylquinic acids showed no significant cytotoxicity at concentrations of 1 -50 μmol x L(-1) as determined by MTT assay. Realtime quantitative PCR revealed that 3-CQA and 3, 5-CQA significantly increased the expression of lipid oxidation-related genes PPARα, CPT-1 and ACOX1 while 3-CQA, 3, 5-CQA and 3,4,5-CQA decreased the expression of lipogenic genes SREBP-1c, SREBP-2, HMGR, ACC, FAS. Overall, 3-CQA, 3, 5-CQA and 3, 4, 5-CQA may be the principal hypolipidemic components in PTF which can decrease intracellular lipid accumulation through up-regulating the expression of lipid oxidative genes and down-regulating the expression of lipogenic genes.
Carcinoma, Hepatocellular
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metabolism
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China
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Cholesterol
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metabolism
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Gene Expression Regulation
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Hep G2 Cells
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Humans
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Lipid Metabolism
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Liver Neoplasms
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metabolism
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Oleic Acid
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Pandanaceae
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chemistry
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Quinic Acid
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analogs & derivatives
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chemistry
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Sterol Regulatory Element Binding Protein 1
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Triglycerides
;
metabolism
10.5-HT(1A) receptors are involved in the modulation of respiratory rhythmical discharge activity in the medulla oblongata slice preparation of neonatal rats.
Zheng QIN ; Zhong-Hai WU ; Xiao-Feng WANG
Acta Physiologica Sinica 2007;59(3):293-298
The present study was carried out to determine the role of 5-HT(1A) receptors in the generation and modulation of basic respiratory rhythm. Neonatal (aged 0-3 d) Sprague-Dawley rats of either sex were used. The medulla oblongata slice was prepared and the surgical procedure was performed in the modified Kreb's solution (MKS) with continuous carbogen (95% O2 and 5% CO2), and ended in 3 min. In cold MKS, a 600-700 microm single transverse slice was cut, which was rostral to the edge of area postrema and retained the hypoglossal nerve roots and some parts of the ventral respiratory group. The preparation was quickly transferred to a recording chamber and continuously perfused with carbogen-saturated MKS at a rate of 4-6 mL/min at 27-29 degrees C. Glass adsorb-electrodes containing Ag-AgCl needle were attached to the ventral roots of the hypoglossal nerve. Respiratory rhythmical discharge activity (RRDA) of the rootlets of hypoglossal nerve was recorded. Ten medulla oblongata slice preparations were divided into two groups. In group I, 5-HT(1A) receptor specific agonist (+/-)-8-hydroxy-2-(di-N-propylamino)tetralin hydrobromide (8-OHDPAT, 20 micromol/L) was added into the perfusion solution for 10 min first, after washing out, the 5-HT(1A) antagonist [4-iodo-N-[2-[4-methoxyphenyl]-1-piperazinyl]ethyl]-N-2-pyridynyl-benzamide hydrochloride] (PMPPI, 10 micromol/L) was applied to the perfusion solution for 10 min. In group II, after application of 8-OHDPAT for 10 min, additional PMPPI was added into the perfusion solution for 10 min. The discharges of the rootlets of hypoglossal nerve were recorded. Signals were amplified and band-pass filtered (100-3.3 kHz). Data were sampled (1-10 kHz) and stored in the computer via BL-420 biological signal processing system. Our results showed that 8-OHDPAT increased the respiratory cycle (RC) and expiratory time (TE) as well as reduced the integral amplitude (IA), but the changes of the inspiratory time (TI) were not statistically significant. PMPPI induced a significant decrease in RC, TE and TI, but the changes of IA were not statistically significant. The effect of 8-OHDPAT on the respiratory rhythm was partially reversed by additional application of PMPPI. Taken together with previous results, 5-HT(1A) receptors may play an important role in the modulation of RRDA in the medulla oblongata slice preparation of neonatal rats.
8-Hydroxy-2-(di-n-propylamino)tetralin
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pharmacology
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Animals
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Animals, Newborn
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Medulla Oblongata
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physiology
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Piperazines
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pharmacology
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Pyridines
;
pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptor, Serotonin, 5-HT1A
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physiology
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Respiration