Objective: To examine the histological outcomes and the predictive value of clinical risks in women with liquid-based Papanicolaou tests (PTs) interpreted as "low-grade squamous intraepithelial lesion, cannot exclude high-grade squamous intraepithelial lesion" (LSIL-H) in comparison with the 2001 Bethesda System (TBS 2001) cytologic categories of low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and atypical squamous cells, cannot rule out a high grade lesion (ASC-H). Methods: The PTs which diagnosed as squamous cell abnormalities, including atypical squamous cells of undetermined significance (ASC-US), LSIL, ASC-H and HSIL, were reviewed by two pathologists who were blinded to the histology results. The diagnose of histology was used as gold standard, and the prevalence of high-risk human papillomavirus (HR-HPV) was examined. Results: Of 49 000 PTs, 88 (0.17%) were rediagnosed as LSIL-H. Before rediagnosed as LSIL-H, these 88 cases were diagnosed as ASC-US (19.32%, 17/88), LSIL (43.18%, 38/88), ASC-H (34.09%, 30/88) and HSIL (3.41%, 3/88). Women who diagnosed as LSIL-H had a higher incidence of cervical intraepithelial neoplasia 2 (CIN-2) /-3 or more severe lesion (CIN-3+) than that in women who diagnosed as LSIL ( P<0.01), but the incidences in these two groups were both lower than that in women who diagnosed as HSIL ( P<0.01). The difference of CIN-2/-3 incidence between LSIL-H and ASC-H was not significant. Conclusion: The similarity of histologic follow-up results between LSIL-H and ASC-H suggested that the similar management decisions should be made for women who were diagnosed as LSIL-H or ASC-H.

Animals ; Antibodies, Monoclonal ; metabolism ; therapeutic use ; Biomarkers, Tumor ; metabolism ; Breast Neoplasms ; metabolism ; pathology ; therapy ; Drug Delivery Systems ; Epidermal Growth Factor ; immunology ; metabolism ; physiology ; Epithelial-Mesenchymal Transition ; GPI-Linked Proteins ; Humans ; Immunotherapy ; methods ; Intercellular Signaling Peptides and Proteins ; Membrane Glycoproteins ; immunology ; metabolism ; physiology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Proteins ; immunology ; metabolism ; physiology

Objective To explore the therapeutic effect of Lingzhu Infusion and Shenqi Capsule sequential therapy in combination with clomiphene citrate(CC)for polycystic ovarian syndrome(PCOS).Methods The 90 PCOS cases in accordance with the enrol- ment standard were randomized into three groups.The treatment groupⅠwas treated with Lingzhu Infusion and Shenqi Capsule se- quential therapy,the treatment groupⅡwas treated with Lingzhu Infusion and Shenqi Capsule sequential therapy and CC,and the control group was treated with CC only.The therapeutic effect was evaluated after two courses of treatment.Results After treat- ment,the changes of TCM syndromes and serum sex hormone indices,ovulation rate and conception rate of the treatment groupⅡwere all superior to those of the treatment groupⅠand the control group.Conclusion The effect of treatment groupⅠis similar to that of control group.The effect of treatment groupⅡis better than that of treatment groupⅠand control group,getting a higher ovulation rate and pregnancy rate.

Objective:To study the influence of morphine on the expression of synaptophysin(SYN)and synapse structure in mice hippocampus,so as to provide pathological evidence for studying the development and treatment of chronic morphine intoxication, addiction and abstinence symptoms of morphine.Methods:Twenty mice were evenly randomized into control group and experiment group.Mice in control group were injected with normal saline(0.1 ml daily)and those in experimental group were injected with morphine(0.1 ml,1 mg daily).Thirty days later the mice in 2 groups were killed and their brain tissues were harvested and made into slices,stained with immunohistochemical techniques(SP)and photographed under the light microscope.The images were analyzed with the image analytical system and the data were statistically analyzed.Results:In the control group,positive staining of SYN was found in the entorhinal area,subiculum,stratum plextiforme,polymorphic layer of gyrus dentatus,stratum oriens,and stratum radiatum of hippocampus;weak positive staining of SYN was noticed in the stratum lacunosum-moleculare of hippocampus;positive staining of SYN was also found the membrane of pyramidal cells and granule cells,with the mean gray scale value of the hippocampal structure being 132.84?8.67.Positively stained neurons was also found in the entorhinal area and the subiculum,with a intensity of(7.80?1.03)/ mm~2.In the experiment group,the suhiculum and polymorphic layers of gyrus dentatus were positively stained for SYN;the entorhinal area,stratum oriens,stratum radiatum and stratum lacunosum-moleculare of hippocampus were strongly positive of SYN;the membrane of pyramidal cells and granule ceils were also strongly positive of SYN,with the mean gray scale value of the hippocampal structure being 116.27?5.70.Strongly stained neurons were also found in the entorhinal area and the subiculum,with the intensity being(11.90?1.45)/mm~2.The number of SYN positive neurons and the intensity of SYN in the experimental group were higher than those in the control group(P

ObjectiveTo evaluate the role of multi-slice CT(MSCT) reconstruction in the diagnosis of intracranial foreign bodies.MethodsMSCT scans were performed in 16 patients with intracranial foreign bodies. The volumetric data were reconstructed in the workstation. 5 patients were detected by CT angiography (CTA) examination. Imaging results were then compared with findings found during surgery.Results11 foreign bodies located in one brain lobe, 3 passed two brain lobes, 2 located in ventricle. Among them, 6 cases were closed to parietal including 2 cases closed to cerebral arterial circle. 14 patients were operated and extirpated 18 foreign bodies. Compared with surgical findings, the orientative accuracy of reconstruction imaging was 100%, qualitative accuracy was 85.7%.ConclusionMSCT reconstruction may display the intracranial foreign bodies from multi-angles. It is propitious to know the characteristics, location and whole shape of the foreign bodies for the surgeon before the operation, accordingly to make a best operation project.

Objective To analyze the status of supply and the affected factors of the home care of the elderly in community of Shenzhen.Methods The investigation was carried out in 78 community health ser-vice centers and 216 elderly from 6 communities using self-designed questionnaire.Results 84% of the el-derly had the demand of home care, 38.1% of them had even received home care services.In 32 demand items of home care, the top 6 were in turn the blood pressure monitoring, the blood sugar monitoring, the health consults, the caregivers guidance, the psychological care, medication instruction.48 of 78 commu-nity health service center provided door-to-door services, 44% of them(home care service center)provided more than 10 projects, 67% of the center provided more than 5 projects, 17% of them provided 2 projects, 30 community health service center did not provide door-to-door service.The main reasons that influenced home care delivery in community were the shortage of nursing staff of home medical care, the risk factors, the medical environment, and the limited health care facilities.Conclusions The elderly has large de-mand to the home care service, home care which the community health center provided is lagging and in short supply.It is imperative to increase the number of community nurses, improve the community home care profession standard and promote stable development of home care.

BACKGROUND: The bone fragments from spinal vertebral fracture can enter into vertebral canal to cause neural functional injury. The restoration of neural function after injury is mainly determined by the character and extent of primary injury, and is related with the spinal injured range. Early imageological analysis can evaluate the degree and range of spinal injury to preliminarily evaluate the therapeutic method and prognosis of the patient.OBJECTIVE: To explore the merit of MRI in the evaluation of function prognosis in compressive fracture induced by spinal trauma or primary osteoporosis to provide a gist for early rehabilitative intervention.DESIGN: A case retrospective study by employing patients with benign vertebral compressive fracture as subjects.SETTING: Department of Imageology of Henan Provincial Hebi Mineral Bureau General Hospital, Department of Radiology of affiliated hospitals of two universities.PARTICIPANTS: Totally 107 cases(123 vertebras) of benign vertebral compressive fracture with complete clinical data including 65 cases of trauma (trauma group, 71 vertebras) and 42 cases of osteoporosis(osteoporosis group, 52 vertebras) were selected from 125 spinal MRI testees of the first affiliated hospital of Beijing Medical University and the first affiliated hospital of Xinxiang Medical College for retrospective analysis.INTERVENTIONS: MRI images of the compressive vertebras in 107 cases (123 vertebras) induced by acute trauma or primary osteoporosis were comparatively analyzed by two vice botanic physicians with blindness method.Vertebral compression was classified into "wedge-shaped" or "flat-shaped" based on the difference between anterior and posterior height of the deformed vertebras and the shapes to observe whether there was bone fragment or not and typical fracture line.RESULTS: MRI manifestation of osteoporosis compression: medullary signal was completely kept in the deformed vertebras with the mixture of high signals. The anterior superior angle of vertebra stuck into vertebral canal. Strip low signal was under the endplate of T1 vertebra. The anterior and posterior vertebral border had normal morphology or slightly sucked with smooth margin. Vertebral vein was clearly displayed. MRI manifestation of traumatic compression: vertebra had relatively even low signal and kept partial medullary signals. Vertebra was obviously deformed with para-vertebral hematoma. There was fracture line or usually accompanied with medullary edema, and cystic degeneration. Vertebral enhancement was an important manifestation of fracture rehabilitative stage, which was the indicator for the judgment of fresh or old fracture.CONCLUSION: MRI can differentiate spinal traumatic fracture and primary osteoporosis fracture, judge fracture time, and preliminarily evaluate the function and prognosis of the patient.

Pulsed field gel electrophoresis(PFGE)is a new type technique of gel electrophoresis which can be used to separate large DNA molecules.It has been widely applied to the karyotype analysis,identification of species groups,genetic orientation and genetic analysis for fungi.This article describes the principle,development and general manipulative procedure of PFGE,and elaborates the application in the molecular research of fungi.

PcoI-PcoR is a quorum-sensing (QS) system influencing the biofilm formation and rhizosphere colonization in Pseudomonas fluorescens 2P24. The expression of the pcoI, a N-acyl-homoserne lactone (AHL) biosynthase gene, is under the regulation of a number of chromosomal factors, such as the GacS-GacA two-component system. In this paper, we investigated the upstream regulators that influence the transcription of pcoI gene using a chromosomal pcoI-lacZ fusion reporter strain PM203. Cosmids containing genomic DNA of the wild-type strain 2P24 were introduced into the reporter strain PM203 (gacA—, pcoI-lacZ) to screen positive transcriptional regulators of pcoI gene. One of them named pP32-24, which contained a 5-kb Pst I functional fragment was selected. Further analysis identified that the pcoI was the gene responsible for the increase of the pcoI-lacZ expression. The expression of pcoI-lacZ reporter was alsoimproved in both PM101 (pcoI-lacZ) and its gacAmutant PM203 after addition of exogenous AHL, indicating that the expression of pcoI is positively regulated by AHL (autoinduction) in strain 2P24. In addition, deletion mutagenesis and complementation experiments demonstrated that the transcriptional regulator PcoR positively controlled the expression of pcoI and the formation of biofilm. These results suggest that, in strain 2P24, the expression of PcoI-PcoR QS system is auto-inducted, and the transcriptional factor PcoR is involved in the regulation of pcoI transcription and the biofilm formation.

BACKGROUND: The cryopreservation of human ovarian tissue has become an attractive method to preserve female fertility. Human ovarian tissue experiences neovasculadzation after transplantation to recover blood supply, cryopreservation and resuscitation technique is a key for the neovascularization of human ovarian tissue following transplantation. OBJECTIVE: To investigate vascular endothelial growth factor (VEGF) expression and microvessel density in human ovadan tissue following novel needle immersed vitrification (NIV) and slow-freezing, to explore the influence of two cryopreservation methods play in the neovascularization of human ovarian tissue after transplantation. METHODS: Eight normal human ovarian tissues from patients with carcinoma of endometrium were cut into 12 fragments in the size of 1.5 mm × 1.5 mm × 1.0 mm, then randomly assigned to 3 groups: fresh control group, NIV group and slow-freezing group. In the NIV group, pieces of ovarian tissue stdps were dehydrated in an equilibration solution consisting of 7.5% ethylene glycol and 7.5% dimethyl sulfoxide in TCM-199 supplement with 20% fetal bovine serum and a vitrification solution consisting of 15% ethylene glycol, 15% dimethyl sulfoxide and 0.5 mol/Lsucrose, then were plunged in liquid nitrogen directly and sealed in liquid nitrogen-filled cryovials. For thawing, the needles holding ovadan tissues were serially transferred into 1.0, 0.5, 0.25 mol/L sucrose solution and TCM-199 supplemented with 20% fetal bovine serum. In the slow-freezing group, pieces of human ovadan cortex fragments were placed in a 1.8-mL cryovial containing 1 mL of TCM-199 medium supplemented with 0.1 mol/L sucrose, 20% fetal bovine serum and 1.5 mol/L dimethyl sulfoxide, the cryovials were placed in the programmable freezer and cryopreserved by preset slow-cooling protocol. For thawing, the ovarian tissue stdps were washed in a stepwise manner: 1.0 mol/L dimethyl sulfoxida + 0.1 mol/L sucrose, 0.5 mol/L dimethyl sulfoxide + 0.1 mol/L sucrose, 0.25 mol/L dimethyl sulfoxJde + 0.1 mol/L sucrose and 0.1 mol/L sucrose. The frozen-thawed and fresh controlled human ovarian tissues were cultured in vitro. The expression of VEGF and CD34, as well as microvessel density, was analyzed by immunohistochemistry. RESULTS AND CONCLUSION: There was patchy and mild expression of VEGF in the stromal cells of all the three groups before and after culture. The expression of VEGF increased and reached peak value after culture for 2 days, began to decrease after culture for 4 days and further attenuated after culture for 6 days in all the three groups. Compared with slow-freezing group, the expression of VEGF in NIV group was closer to that in fresh control group. Microvessel density of all the three groups increased and reached peak value after culture for 2 days, and the microvessel density of fresh control group and NIV group was significantly higher than that of slow-freezing group (P < 0.05). The microvessel density of slow-freezing group after culture for 4 days and that of all the three groups after culture for 6 days significantly decreased compared with after culture for 2 days (P <0.05). NIV is superior to slow-freezing to preserve stromal cells and extracellular matrix of human ovarian tissue, and plays less influence in VEGF expression and angiogenesis in human ovarian tissue.