Objective To compare the cognitive function of type 2 diabetic cases with diabetic microangiopathy and that of type 2 diabetic cases without diabetic micro- angiopathy, to provide evidence for clinical instruction. Methods There were 20 type 2 diabetic cases with diabetic micro-angiopathy and 23 type 2 diabetic cases without diabetic micro-angiopathy. The cognitive function were evaluated by the scores of MMSE, vocabulary learning, vocabulary memory, vocabulary recognition, visual memory, thought judgment, spatial skill, verbal fluency, construction speed and spatial executive function. Results The scores of MMSE and thought judgment ability in type 2 diabetic cases with diabetic micro-angiopathy were significantly lower than that in normal control group(P < 0. 05). Conclusions There was cognitive functions impair in type 2 diabetic cases with diabetic micro-angiopathy group, The corresponsive nursing strategy must be carried out.

Humans ; Liver Cirrhosis ; diagnosis

OBJECTIVE: To explore the relationship between the expression changes of cytokines, interleukin-6 (IL-6), interleukin-8(IL-8), and tumor necrosis factor-alpha (TNF-alpha), and the wound time following explosive injury to rabbit's chest. METHODS: The rabbit's model of explosive injury was established. The expression levels of IL-6, IL-8 and TNF-alpha in the plasma were detected by ELISA method at different wound time (0.5-12h). RESULTS: The level of IL-6 increased at 3h after wounding and reached peak at 6 h. The level of IL-8 increased at 1 h and reached peak at 6 h. The level of TNF-alpha increased at 0.5 h and reached peak at 3 h. CONCLUSION IL-6, IL-8 and TNF-alpha have a time-related expression after explosive injury.
Animals ; Enzyme-Linked Immunosorbent Assay ; Explosions ; Interleukin-6/metabolism* ; Interleukin-8/metabolism* ; Rabbits ; Thoracic Injuries/metabolism* ; Tumor Necrosis Factor-alpha/metabolism*

ObjectiveTo detect specific antigens of neural cells in amniotic epithelial cells(AECs) in rats. MethodsAECs were dissociated and purified from the amnion of pregnancy 12—14 d rats. The expression of specific markers of neural stem cells (Nestin, Musashi) and differentiated cells (MAP-2,NSE,GFAP) and ChAT, NT-3 in the AECs were detected by immunocytochemistry. ResultsThe cultured AECs displayed positive immunoreactivity to MAP-2, NSE, GFAP, Nestin and Musashi. In addition, the cells also demonstrated immunoreactivity to ChAT and NT-3. ConclusionAECs are similar with neural cells and it may be useful as a sustained source to improve outcome of neural stem cells transplantation.

ObjectiveTo investigate an effective method to isolate neural stem cells(NSCs).MethodsNSCs were dissociated by digestion with trypsin, EDTA and different doses of Dispase,and serum-free culture techniques and immunohistochemistry techniques were used to verifying the dissociated.ResultsA lot of single neural stem cells were obtained by using Dispase to digest neurosphere, and the cells could keep its structure and morphology.ConclusionIt is an ideal method by using Dispase to digest neurosphere for isolating NSCs.

The synaptic connection from high vocal center (HVC) to robust nucleus of the arcopallium (RA) is a pivotal part of vocal motor pathway in songbirds. Electrophysiological properties of HVC-RA synaptic transmission in adult male zebra finch (Taeniopygia guttata) in vivo was investigated by using field potential recording method. Following electrical stimulation of HVC, the evoked field potentials recorded in RA were feeble. The results showed that the remarkable paired-pulse facilitation was induced by paired-pulse stimulation at HVC-RA synapses. The results also showed that the evoked field potentials were significantly decreased after a conditioning tetanic stimulation and finally recovered gradually within 15 min, which indicates tetanic stimulation-induced transient depression is present at HVC-RA synaptic transmission. These results suggest that properties of synaptic transmission in this pathway might play a role in controlling song production.
Animals ; Electrophysiological Phenomena ; physiology ; Evoked Potentials ; Finches ; physiology ; High Vocal Center ; physiology ; Male ; Prosencephalon ; cytology ; physiology ; Synaptic Transmission ; physiology ; Vocalization, Animal ; physiology

Aged ; B-Lymphocytes ; pathology ; Histiocytic Sarcoma ; complications ; Humans ; Lymphoma, B-Cell ; etiology ; Lymphoma, Large B-Cell, Diffuse ; etiology ; pathology ; Male

Adult ; Antigens, Neoplasm ; metabolism ; Gastrointestinal Neoplasms ; metabolism ; pathology ; surgery ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; MART-1 Antigen ; Male ; Neoplasm Proteins ; metabolism ; Osteoclasts ; pathology ; S100 Proteins ; metabolism ; Sarcoma, Clear Cell ; metabolism ; pathology ; surgery ; Soft Tissue Neoplasms ; metabolism ; pathology ; surgery

Course teaching for international students in many domestic universities is in English.Some practice and experience of the department of nuclear medicine in Tianjin medical university are introduced from the aspects of teaching course,concerning preparations before class,teaching process and teaching techniques,which will give references to nuclear medicine teaching for international students.

Objective To investigate the prognostic factors of cervical high-grade squamous intraepithelial lesions treated by cold knife conization with negative margin.Methods Two hundred and sixty-six women with cervical high-grade squamous intraepithelial lesions treated by cold-knife conization with negative margins at Beijing Hospital between Jan 1999 and Jan 2004 were analyzed retrospectively.All patients were followed up with cytology,high-risk human papillomavirus(HPV)test and eolposcopy if necessary.Results The cervical CIN recurrence rate was 8.6% with no incidence of invasive cervical cancer after a median follow-up of 46 months.The recurrence was related to the grade of lesions and gland involvement pathologically.One of 20(5.0%)cases with cervical intraepithelial neoplasia(CIN)Ⅱ,9 of 164(5.5%)cases with CIN Ⅲ(excluding carcinoma in situ,CIS)and 13 of 82(15.8%)cases with CIS recurred(P