1.Detection of MYD88 mutation in lymphoma by PCR-high resolution melting curve analysis.
Di XUE ; Jiang LIN ; Gao-fei XIAO
Chinese Journal of Hematology 2013;34(1):71-73
Adult
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Aged
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Aged, 80 and over
;
DNA Primers
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Female
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Humans
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Lymphoma
;
genetics
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Male
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Middle Aged
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Mutation
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Myeloid Differentiation Factor 88
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genetics
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Polymerase Chain Reaction
2.Isolation and Identification of Osmotolerant Yeast from "Swollen Can" Soy Sauce
You-Sheng OUYANY ; Xiao-Bao XIE ; Jiao-Di CHEN ; Hua XUE ; Yi-Ben CHEN ;
Microbiology 1992;0(04):-
Three osmotolerant yeasts were isolated from three batches of "swollen can" soy sauce produced by a Guangdong condiment plant. These strains grew faster in the media containing 50%~60% glucose or 15% NaCl than in common yeast media. The three yeasts were identified as Pichia etchellsii by using morphological characteristics, physiological and biochemical tests.
3.Application value of single intensifying screen cassette in examination of Kashin-Beck disease through X-ray photographing
Xue-song, WANG ; Xiao-di, LU ; Wen-jing, ZHANG ; Xu, ZHANG
Chinese Journal of Endemiology 2013;(3):328-330
Objective To explore the application value of single intensifying screen cassette in examination of Kashin-Beck disease through X-ray photographing.Methods Single intensifying screen cassette and traditional changing bag with exposure conditions were used to check right wrists of 110 children in Kashin-Beck disease areas.The FJ personal dosimeter was used to measure exposure dose.Photo graphing quality and diagnosis effect were assessed.Results The radiation dose of children in single intensifying screen cassette group was (207 ± 39)μsv/h,while in the traditional changing bag group was (1425 ± 63)μsv/h.The difference between the two groups was statistically significant(t =140.16,P < 0.05).The high-quality photograph rate of the two methods was 94.55% (104/110) and 92.73% (102/110),respectively,and the difference was not statistically significant(P > 0.05).The good photo rate in the single intensifying screen cassette was 96.36% (94/110),which was significantly higher than that of the traditional changing bag group[44.55% (49/110),x2 =70.92,P < 0.01].Conclusions X-ray radiation dose in single intensifying screen cassette group is smaller than that of the traditional changing bag group,and the image quality of the radiograph of the new method is also superior.It has a good practical value in the X-ray examination of Kashin-Beck disease.
4.Off-pump coronary artery bypass grafting in patients over the age of seventy.
Song XUE ; Bo XIE ; Sha LIU ; Ming-Di XIAO
Chinese Journal of Surgery 2004;42(11):661-663
OBJECTIVETo discuss the choice of surgical methods and the safety and efficacy of off-pump coronary artery bypass grafting (OPCAB) in elderly patients.
METHODSFrom Sept. 1997 to Feb. 2003, 63 cases over the age of seventy (including seventy) undertook OPCAB in our department. We compared the clinical data of those patients with that of 94 cases undertook conventional coronary artery bypass grafting (CABG) at the same age group and that of 58 cases younger than seventy received OPCAB.
RESULTSThe clinical outcomes of OPCAB is better than that of CABG in elderly patients concerning inotropic drug, postoperative transfusion, re-operation, intubation time, complications incidence and in-hospital mortality. Furthermore, there is no significant difference of complication incidence and in-hospital mortality between the elderly OPCAB group and the younger OPCAB group.
CONCLUSIONSOPCAB is a safe and efficacious method of myocardial revascularization in the elderly.
Aged ; Aged, 80 and over ; Blood Transfusion ; statistics & numerical data ; Cardiotonic Agents ; administration & dosage ; Coronary Artery Bypass, Off-Pump ; mortality ; standards ; Hospital Mortality ; Humans ; Myocardial Revascularization ; Postoperative Complications ; epidemiology ; Retrospective Studies
5.PhaseⅢstudy on late course accelerated fractionated irradiation combined with concurrent chemotherapy for esophagus squamous cell carcinoma
Kuai-Le ZHAO ; Xue-Hui SHI ; Guo-Liang JIANG ; Xiao-Mao GUO ; Wei-Qiang YAO ; Gen-Di WU ; Long-Xiang ZHU ;
Chinese Journal of Radiation Oncology 1992;0(04):-
Objective To evaluate the role of late course accelerated fractions.ted irradiation(LCAF) combined with concurrent chemotherapy in the management of esophageal carcinoma.Methods From March 1998 to July 2000,111 eligible patients were randomized into LCAF alone group(LCAF,57 patients)or LCAF plus concurrent chemotherapy group(LACF-CT,54 patients).The radiotherapy regimen was identical in the two groups,consisting of conventional fractionation in the first 2/3 course and accelerated fractionation in the second 1/ 3 course to a total dose of 68.4 Gy/41 Fx/44 d.Chemotherapy regimen consisted of four eycles of cisplatin 25 mg/ (m~2?d)plus fluorouracil 600 mg/(m~2?d)on day 1 to 3 every 4 weeks and was delivered on the first day of radiotherapy.Results The median follow-up time was 67.1 months(range 47.6-76.4 months).The 1-,3-,5- year survival rate was 67%,44% ,40% and 77%,39% 28% in LACF-CF and LEAF group,respectively(P =0.310).Grade 3+4 acute side-effact was 42% and 25% in LCAF-CT and LCAF group,respectively(P<0. 05),with 3 treatment-related deaths in the LCAF-CT group.Conclusions Late course accelerated fractionated irradiation combined with concurrent chemotherapy has a trend towards improving the survival,at the cost of increasing acute side-effect.Its role needs further confirmation by larger sample studied in randomization.
6.Qualitative and quantitative analysis of fluoxetine hydrochloride by 19F NMR.
Bai-Qin YANG ; Er-Li KONG ; Xiao-Di XUE ; Shou-Qian ZHAO ; Shrong-Shi LIN
Acta Pharmaceutica Sinica 2012;47(5):630-633
The chemical shift of fluoxetine hydrochloride appears at delta 14.15 in 19F NMR analysis. The delta moved upfield slightly from 14.158 to 14.145 when the concentration of solution became diluted from 2.00 to 0.05 mmol x L(-1). Spiking test was suggested to confirm the existence of the compound for qualitative analysis. 19F NMR detection sensitivity test illustrated that a concentration of 17 mg in 1 L water could be detected while the sample was scanned 500 times with optimum parameters. In quantitative analysis, standard curve of concentration versus fluorine signal intensity was proposed to determine the amount of fluoxetine. Long capillary tube containing trifluoroacetic acid was used as internal standard for the integration measurements and straight line was obtained with good fitting. Direct additions of trifluoroethanol to fluoxetine solutions gave a poorer standard curve.
Fluorine
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chemistry
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Fluoxetine
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analysis
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Trifluoroacetic Acid
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analysis
7.Chemical constituents of unsaponifiable matter from seed oil of Momordica cochinchinensis.
Lian-Di KAN ; Quan HU ; Zhi-Mao CHAO ; Xiao SONG ; Xue-Li CAO
China Journal of Chinese Materia Medica 2006;31(17):1441-1444
OBJECTIVETo study the chemical constituents of unsaponifiable matter from the seed oil of Momordica cochinchinensis.
METHODThe fatty oil from the seeds of M. cochinchinensis was extracted with petroleum ether, and the saponification was carried out with potassium hydroxide. The unsaponifiable matter was isolated and purified by silica gel column chromatography, and the structures of their constituents were elucidated by means of IR, MS, 1H-NMR, and authentic chemicals.
RESULTKarounidiol (1), isokarounidiol (2), 5-dehydrokarounidiol (3), 7-oxodihydrokarounidiol (4), beta-sitosterol (5), stigmast-7-en-3beta-ol (6), and stigmast-7,22-dien-3beta-ol (7) were elucidated.
CONCLUSIONThese compounds were found in this plant for the first time.
Molecular Structure ; Momordica ; chemistry ; Oleanolic Acid ; analogs & derivatives ; chemistry ; isolation & purification ; Plant Oils ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Seeds ; chemistry ; Triterpenes ; chemistry ; isolation & purification
8.The effect of oral alpha-lipoic acid in overweight/obese individuals on brachial-ankle pulse wave velocity and supine blood pressure:a randomized,crossover,double-blind,placebo-controlled trial
Yong-Di HUANG ; Nong LI ; Wei-Guo ZHANG ; Xiao-Juan HU ; Qian WANG ; Chen-Chen WANG ; Rui-Wei XU ; Kai YAN ; Xue-Yan HOU ; Naerkeze ; Xiao-Ling WANG ; Wei-Li YAN
Chinese Journal of Epidemiology 2011;32(3):290-296
Objective To investigate the effect of oral alpha-lipoic acid (ALA) supplement on brachial-ankle pulse wave velocity (baPWV),supine systolic blood pressure (SBP) and diastolic blood pressure (DBP) in overweight/obese individuals.An 8-week double-blind,randomized,placebo-controlled and cross-over trial with a 4-week washout between cross-over periods.Methods Sixty-three males and 40 females aged 22-57 years old who met the inclusion criteria as (1) Han ethnicity;(2) 20-60 years old;(3) BMI≥25 kg/m2 and having at least one of the following risk factors:borderline hypertension (130 mm Hg≤SBP<140 mm Hg and/or 85 mm Hg≤supine DBP<90 mm Hg),dyslipidemia(fasting total cholesterol≥5.2 mmol/L or HDL-C<1.04 mmol/L),or impaired fasting glucose (6.1 mmol/L≤fasting glucose<7.0 mmol/L);(4)Not on any antioxidant gender.Group 1 received 8 weeks ALA (1200 mg/day) followed by 4-week washout period and followed by another 8 weeks placebo;while Group 2 received 8 weeks placebo (1200 mg/day)followed by 4-week washout period,and followed by ALA treatment for 8 weeks.BaPWV and supine blood pressure were measured at the beginning of 1st phase and 2nd phase and at the endpoint of the whole trial.Mixed effect linear regression model was performed to compare the change of baPWV and supine blood pressure between ALA group and placebo group.Results BaPWV decreased -33.03 cm/s ± 130.70 cm/s for ALA group and increased 5.66 cm/s ± 139.89 cm/s for placebo group,supine systolic blood pressure decreased -4.09 mm Hg±9.18 mm Hg for ALA group and -2.32 mm Hg±8.16 mm Hg for placebo group.Supine diastolic blood pressure decreased -1.29 mm Hg ± 6.55 mm Hg for ALA group and -0.48 mm Hg±6.63 mm Hg for placebo group.These three mix-effect models did not show significant effect of ALA treatment after adjustment on baseline values,sex,age,treatment sequence or period.Conclusion The current trial did not provide evidence that oral intake of ALA for 8 weeks had significant effects on lowering baPWV,supine systolic blood pressure or supine diastolic blood pressure.
9.Investigation about prevention behavior for dust workers in machinery, ceramic, and metallurgy industry.
Fu-hai SHEN ; Qing-kun MA ; Shu-yu XIAO ; Feng-tao CUI ; Qing-di MENG ; Xiu-qing YANG ; Hui-sheng QI ; Xue-yun FAN ; San-qiao YAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(1):59-61
OBJECTIVEThe purposes of this thesis were to study the behavior about workers exposed to dust and provide scientific basis for health promotion.
METHODSWe designed a questionnaire and carry it on the 746 dust workers in the 3 representative corporations of Machinery, Ceramic, and Metallurgy Industry. All data were input into computer. And a database was established with Excel. SPSS11.5 statistical analysis software was used to analyze the influence on protecting behavioral between the application of qualifications, different jobs, training or protection, and other aspects etc.
RESULTSThe rates were 94.4% and 75.3% about the regular physical examination and requirements for protective equipment. The rate of choosing an effective way of protection was generally low (15.4%). There was significant difference for among different educational background workers (P < 0.01). The rates of choosing an effective way of protection (20.3%), the regular physical examination (98.3%) and requirements for protective equipment (86.4%) in the dust workers who participated in the training of dust protection were superior than those who did not participated in the training. There was the significant difference (P < 0.05, P < 0.01). There was the significant difference for the rate of effective way of protection, regular physical examination, and requirements for protective equipment among the different corporations (P < 0.05).
CONCLUSIONSDust workers' using rate about the choosing an effective way of protection was generally low in Machinery, Ceramic, and Metallurgy Industry. Those who were not educated had a lower using rate about the protection behavior, regular physical examination, and requirements for protective equipment than those educated.
Adolescent ; Adult ; Ceramics ; Choice Behavior ; Dust ; prevention & control ; Female ; Humans ; Industry ; Male ; Metallurgy ; Middle Aged ; Occupational Exposure ; prevention & control ; Respiratory Protective Devices ; utilization ; Young Adult
10.The expression of a novel estrogen receptor, GPR30, in epithelial ovarian carcinoma and its correlation with MMP-9.
Hui-Di LIU ; Yan YAN ; Xue-Feng CAO ; Pei-Zhu TAN ; Hai-Xia WEN ; Chun-Mei LV ; Xiao-Mei LI ; Guo-Yi LIU
Acta Physiologica Sinica 2010;62(6):524-528
The aim of the present study is to investigate the expression of a novel estrogen receptor, G protein-coupled receptor 30 (GPR30) and its correlation with matrix metalloproteinases-9 (MMP-9) in epithelial ovarian cancer (EOC). Ovary tissues were obtained from 39 female patients, including 30 cases of EOC and 9 cases of benign ovarian tumor. Four normal ovary tissues were used as control. Immunohistochemical staining was used to detect the expressions of GPR30 and MMP-9. Chi square test, Fisher's exact test and Spearman's rank correlation analysis were used for statistical analysis. The results showed that GPR30 overexpression rate in EOC cases was significantly higher than those in benign ovarian tumor and normal ovary cases. Whereas MMP-9 overexpression rate in EOC cases was significantly higher than that in normal ovary cases, without any difference to that in benign ovarian tumor cases. To demonstrate the relationship between GPR30 and clinicopathological variables of EOC, we further analyzed the pathology type, FIGO stage and age of patients sampled in our study. The analysis showed there were significant differences of GPR30 overexpression rate among various pathology types and different FIGO stages (P<0.05), and no significant difference of both GPR30 and MMP-9 among three age groups (P>0.05). Moreover, GPR30 expression was positively correlated with MMP-9 (r(s)=1.000, P=0.002). These results suggest that GPR30 may be involved in the invasion and metastasis of EOC, being a potential index of EOC early diagnosis and malignancy grade prediction.
Adult
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Aged
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Biomarkers
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metabolism
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Cystadenocarcinoma, Serous
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metabolism
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Female
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Humans
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Matrix Metalloproteinase 9
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metabolism
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Middle Aged
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Neoplasms, Glandular and Epithelial
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metabolism
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Ovarian Neoplasms
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metabolism
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Receptors, Estrogen
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metabolism
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Receptors, G-Protein-Coupled
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metabolism
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Young Adult