2.Review of the Ecological Risks of Genetically Modified Trees
Ying-Jie HOU ; Bing-Yu ZHANG ; Xiao-Hua SU ;
China Biotechnology 2006;0(12):-
Delightful achievements have been obtained in forestry genetic breeding since the application of transgenic technology in this field during the past 20 years. Field trials of some genetic modified (GM) trees have been carried out, and some GM trees have been commercialized. Meanwhile, the risks of ecological safety caused by GM trees have raised attention in the public gradually. These issues mainly include the horizontal transfer and vertical flow of foreign genes, and the potential effects on insects, soil ecosystems and virus. The current status of field trials, commercial applications and the potential ecological risks of GM trees were summarized. Then the prospects of GM trees were also presented.
3.Experimental study on effect of chemotherapy combined ginsengnoside Rg3 in treating pulmonary carcinoma.
Cheng YI ; Xiao-bing HUANG ; Mei HOU
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(1):58-59
Angiogenesis Inhibitors
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pharmacology
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therapeutic use
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Animals
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Antimetabolites, Antineoplastic
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therapeutic use
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Antineoplastic Agents, Phytogenic
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pharmacology
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therapeutic use
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Deoxycytidine
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analogs & derivatives
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pharmacology
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therapeutic use
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Drug Therapy, Combination
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Female
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Ginsenosides
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pharmacology
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therapeutic use
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Lung Neoplasms
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drug therapy
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pathology
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Mice
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Mice, Inbred C57BL
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Neoplasm Transplantation
4.Oxymatrine-induced apoptosis of HepG2 cells:the possible mechanism
Ying HOU ; Wei CAO ; Shui-Bing LIU ; Xiao-Nan ZHANG ; Xu-Bo LI ; Qiong TIAN ;
Academic Journal of Second Military Medical University 1985;0(06):-
Objective:To investigate the effects of oxymatrine(OM)on the apoptosis of human hepatoma HepG2 cells and its possible mechanisms.Methods:HepG2 cells were treated with different concentrations of OM.The proliferation inhibition was measured by MTT assay and the apoptosis of HepG2 cells were examined by Hochest staining method.Flow cytometry was used to analyze the cell cycle distribution and apoptosis rate.The expression of caspase-3,Bcl-2,Bcl-x_L and Bax proteins was assayed by Western blotting assay.Results:OM inhibited HepG2 cells growth in a time-and dose-dependent manner.After treatment with OM for 24 hours,some cells appeared typical apoptotic characteristics and the apoptosis rate was increased. Treatment with OM also increased caspase-3 activity and Bax expression in HepG2 cells,and decreased the expression levels of Bcl-2 and Bcl-x_L.Conclusion:OM can induce HepG2 cell apoptosis,which may be related to the down-regulation of PI3K/Akt signal pathway,suppression of Bcl-2 and Bcl-x_L activity,and activation of caspase-3.
5.Prognostic Value of EZH2,Ki-67 and CD34 Expression in Patients with Surgically Treated Colorectal Cancer
fa Hong HOU ; hui Yong SU ; yuan Ju BU ; Xiao WANG ; zong Bing HOU
Journal of Sun Yat-sen University(Medical Sciences) 2017;38(6):866-872
[Objective]To evaluate the expression of enhancer of zeste homolog 2(EZH2),Ki-67 and intratumoral microvaseu?lar density(iMVD)in human colorectal cancer(CRC),and discuss their relationship with the biological behavior and prognosis.[Methods]The expression of EZH2,Ki-67 and iMVD(labeled by CD34 protein)was measured with immunohistochemical MaxVision method in CRC patients followed up over 5 years.[Results]Positive expression rates of EZH2,Ki-67 and iMVD were 40.91%(45/110),57.27%(63/110)and 46.36%(51/110),respectively. EZH2 expression was positively correlated with distant metastasis(P=0.024). Ki-67 expression was positively correlated with tumor size,infiltration depth,AJCC stage and differentiation(P=0.033~0.015). The iMVD expression was positively correlated with infiltration depth ,AJCC stage and differentiation (P=0.016~0.034). Spearman correlation analysis showed that EZH2 expression was positively correlated with Ki-67 expression(r=0.195,P=0.041), whereas negatively correlated with iMVD expression. Ki-67 expression had a positive correlation with iMVD(r=0.213,P=0.025). Multivariate analysis suggested that the EZH2 expression was an adverse independent factor for survivals of CRC patients(HR 1.965, 95%CI:1.019-3.789,P=0.044 for OS).[Conclusion]EZH2 expression was significantly correlate with the proliferation of tumor cells and may plays an important role in the metastasis and prognosis of CRC.
6.Surgical outcomes of robot-assisted partial nephrectomy in 12 cases
Linhui WANG ; Huamao YE ; Bin XU ; Bing LIU ; Jiatao JI ; Liang XIAO ; Xia SHENG ; Lili WANG ; Xin CHENG ; Jiong HOU ; Jianguo HOU ; Yinghao SUN
Chinese Journal of Urology 2012;(11):814-817
Objective To summarize the surgical experience in robotic-assisted laparoscopic partial nephrectomy,and to investigate the efficacy and safety of this surgery.Methods The clinical data of 12 patients who underwent robot-assisted laparoscopic partial nephrectomy in Changhai Hospital from March to July in 2012 were analyzed.All the patients were male and the age range was 43-66 years.In 4 cases the tumors were in the left kidney,and 8 in the right.In 7 cases the tumors were in the dorsal part of the kidney,and 2 in the ventral part.There were 3,5 and 4 cases in the upper,middle and lower pole of the kidney respectively.Preoperative GFR test was normal in all cases.Kidney CT scan showed the maximum diameters of the tumors were 2.0-5.8 cm,with an average of 3.3 cm.The pre-operative stages in all cases were T1N0M0.Results The surgery was successfully completed in all cases.The mean duration of the surgery was 160-310 min,with an average of 242 min.The blood loss was 30-300 ml,with an average of 135 ml,and the intraoperative blood transfusion was unnecessary.The warm ischemia time was 20-49 min,with an average of 31 min.There was no intraoperative morbidity,and no conversion to open surgery.The postoperative length of hospitalization was 9-31 d,with an average of 14 d.Gross hematuria arose in 1 patient at 1 week after the surgery.The post-operative pathology showed renal clear cell carcinoma with Furhman Grade Ⅱ in 11 cases,and renal angiomyolipoma in 1 case.The maximum diameters of the tumors were 2.0-5.0 cm,with an average of 3.5 cm.The tumor resection margin was negative in all cases.Conclusions Robot-assisted laparoscopic partial nephrectomy is safe and effective for local renal tumors.This surgery has significant advantage over traditional laparoscopic partial nephrectomy,in terms of the resection of the renal tumors and the reconstruction of the kidney.
7.The experimental study of mechanism of integrin in hypertrophic scar contracture.
Zheng-yu HE ; Xiao-bing HOU ; Zhi-hong FAN
Chinese Journal of Plastic Surgery 2006;22(1):38-40
OBJECTIVETo study the mechanism of integrin in hypertrophic scar.
METHODSFibroblasts from 10 samples of human hypertrophic scars was cultured, FQ-PCR assay was applied to detect mRNA expression of alpha-SMA in hypertrophic scar fibroblasts after integrin and FAK antibody blocking.
RESULTSmRNA of alpha-SMA in fibroblasts expressed obviously lower after integrin and FAK antibody blocking than that of control groups ( P < 0.05).
CONCLUSIONThrough accelerating the synthesis of alpha-SMA, integrin and FAK play an important role in contracture of hypertrophic scar.
Actins ; biosynthesis ; Adult ; Cells, Cultured ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Contracture ; metabolism ; pathology ; Fibroblasts ; metabolism ; Focal Adhesion Kinase 1 ; biosynthesis ; Humans ; Integrins ; biosynthesis ; RNA, Messenger ; metabolism ; Young Adult
8.Determination of arsenic content in Changtong oral liquid by inductively coupled plasma- mass spectrometry.
Dan GUO ; Xi-xiao YANG ; Lian-bing HOU
Journal of Southern Medical University 2006;26(9):1311-1312
To determine arsenic content in Changtong oral liquid, inductively coupled plasma-mass spectrometry (ICP-MS) was employed, which generated linear calibration curves in the range of 5-25 ng/ml for As (r=0.9998). The average recovery of As was 98.94% (n=5, RSD=2.58%).
Arsenic
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analysis
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Drug Contamination
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Drugs, Chinese Herbal
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analysis
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chemistry
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Mass Spectrometry
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methods
9.Senile dementia: differentiation of syndromes according to meridians based on the theory of "cerebral collaterals injury by toxin".
Li ZHOU ; Yun-Ling ZHANG ; Xiao-Bing HOU ; Hui HU
Chinese Acupuncture & Moxibustion 2012;32(11):1031-1034
The pathogenesis and treatments based on meridian differentiation of senile dementia are discussed through analyses and researches on the theory of "cerebral collaterals injury by toxins" and "collateral diseases". The symptoms of "Cerebral collaterals injury by toxins" are preliminary characterized by toxins and blood stasis occluding brain collaterals. "Cerebral collateral injury by toxins" and "Governor Vessel occlusion by blood stasis" are taken as the major pathogeneses of senile dementia. And the treatment should be focused on clearing the collaterals. Clearance acting as reinforcing as well as to clear and modify the Governor Vessel are taken as crucial sections in the treatment of senile dementia based on meridian differentiation. It is also the application of acupuncture-moxibustion intervention in senile dementia based on the theory of "cerebral collateral injury by toxins", which expands the application of the theory concerning "collateral diseases" in disease prevention and treatment with acupuncture-moxibustion.
Acupuncture Therapy
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Alzheimer Disease
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diagnosis
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metabolism
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therapy
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Brain
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drug effects
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metabolism
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Diagnosis, Differential
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Humans
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Meridians
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Toxins, Biological
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metabolism
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pharmacology
10.Antimicrobial Peptides: Antibacterial Mechanism and Therapeutic Use
Xiao-Shu HOU ; Zong-Li HU ; Guo-Ping CHEN ; Yong LI ; Bing-Qin WANG ; Zhi-En LI ;
Microbiology 1992;0(01):-
Antimicrobial peptides, a cluster of small peptides secreted by the majority of creatures, have been demonstrated with activity against a wide range of microorganisms including bacteria, protozoa, yeast, fungi, viruses and even tumor cells. These peptides have some features such as broad spectrum , high effi-cacy and stability, little drug resistance. A lack of new antibiotics combined with emerging multi-drug resis-tance issues demands that new antimicrobial strategies be explored for treating these infections. It has been proposed that the antimicrobial peptides might form the foundation for a new class of clinically useful an-timicrobials. We review the advantages of these molecules in construction features and bioactivity, with the focus on the mechanism and clinical applications.