1. Effects of over-expression and silenced lentivirus Tmubl vectors transfection on the proliferation of rat liver cell line BRL-3A
Medical Journal of Chinese People's Liberation Army 2016;41(1):12-16
Objective To investigate the role of Tmubl protein in liver cell proliferation. Methods Using silenced lentivirus (LV)-Tmubl vector, over-expression LV-Tmubl vector, and corresponding empty vectors to transfect rat’s liver cell line BRL-3A to obtain stable transfected cells, which bear the expression of Tmubl protein as detected by Western blotting, cell proliferation with MTT, and cell cycle with flow cytometry. Results The Tumbl protein expression in the hepatocytes transfected by LV-Tmubl was increased significantly and that by LV-Tmubl-RNAi decreased significantly, with a statistically significant difference compared with normal hepatocytes (P<0.01). The proliferation was fastest in hepatocytes transfected by LV-Tmubl and slowest in those by LV-Tmubl-RNAi, and the difference was statistically significant (P<0.01). The G2+S phase was longer in hepatocytes transfected by over-expression LV-Tmubl vectors than in those by silenced LV-Tmubl vectors, and the difference was statistically significant (P<0.01). Conclusion Tmubl protein can promote the hepatocyte proliferation in BRL-3A cell line.
2. Research on the interaction of protein tmub1 and securin after partial hepatectomy
Medical Journal of Chinese People's Liberation Army 2014;39(11):864-867
Objective To investigate the interaction of Tmub1 and securin protein after partial hepatectomy. Methods A total of 30 adult male SD rats were randomly divided into 3 groups (10 each) according to the time after hepatectomy, i.e, 0, 24 and 48 h. Partial hepatectomy was performed in the rats in the first group, and then the primary hepatocytes were directly harvested by liver perfusion. Partial hepatectomy was also performed in the rest two groups at postoperative 24 and 48 h. Then the primary hepatocytes were also harvested respectively after liver perfusion. One part of primary hepatocytes was cultured for one night and for the observation of the colocalization of Tmub1 and securin protein with immunofluorescence. The other part of hepatocytes were used for co-immunoprecipitation and Western blotting to detect the expression of protein Tmub1/securin. Results Immunofluorescence and confocal laser scanning microscopy showed that there were almost no Tmub1 and securin proteins in the nucleus 0 h after liver resection, while a great amount of Tmub1/securin proteins were found to be co-localized in the nucleus 24 and 48 h. Co-immunoprecipitation and Western blotting showed that the expression of Tmub1/securing protein significantly enhanced in 24-48 h after operation compared with that at 0 h (P<0.01), while the difference between 24 h and 48 h showed no statistically significant difference (P>0.05). Conclusion Tmub1 hardly combines with securin protein in normal hepatocytes, but it combines with securin protein when it migrates from cytoplasm into nucleus after hepatectomy, thus inhibiting the proliferation process of hepatocytes.
3.Reliability and correlation of in vivo heptic shimming of liver and water suppression with 3.0T proton MRS
Li XU ; Changhong LIANG ; Zaiyi LIU ; Zhenjun ZHAO ; Biao HUANG ; Qiongxin ZENG ; Yuanqiu XIAO
Chinese Journal of Medical Imaging Technology 2009;25(7):1208-1210
Objective To assess the reliability of auto-shimming line width (LW) and water suppression rate (WS), and the correlation between them. Methods GE Signa Excite HD 3.0T system and eight-channel torso phased-array coils with PRESS sequence were performed in 38 volunteers. Liver spectra were collected with TR of 1500 ms, TE of 30 ms, VOI of 2 cm×2 cm×2 cm, NSA of 64 times. Spectroscopy routine auto-shimming pre-scanning program was executed and the values of LW and WS were recorded. Then another spectroscopy routine auto-shimming pre-scanning program was performed repeatedly and 38 groups of data were obtained totally. Intra-class correlation coefficients (ICC), coefficient of variation (CV) and significance test were conduced on 38 groups of LW and WS data. Spearman rank correlation analysis was used to assess the correlation of LW and WS. Results ①The ICC of LW and WS was 0.862 and 0.961 (both P<0.0001), respectively, while the value of CV was 0.20, 0.18, 0.09 and 0.08, respectively. Significant difference was not observed; ②The value of correlation coefficient was -0.659, -0.485 (both P<0.0001), respectively. Conclusion ①The reliability is excellent for in vivo liver 3.0T 1H-MRS and WS appears relatively stable; ②Indexes of LW correlate with WS moderately, and it seems the smaller the value of LW is, the easier to achieve higher WS.
4.The clinical experience of T3-4 thoracic sympathectomy in the treatment of hyperhidrosis
Biao XU ; Junxu WU ; Xiao ZHOU ; Jiming SHA ; Xudong ZHAO ; Kaihu SHI
Chinese Journal of Postgraduates of Medicine 2014;37(2):24-26
Objective To summarize the clinical experience of T3-4 thoracic sympathectomy in the treatment of primary hyperhidrosis.Methods The clinical data of 80 patients with primary hyperhidrosis who underwent T3-4 thoracic sympathectomy were analyzed retrospectively.Results The operation was successfully performed on all patients.The symptom of palmar hyperhidrosis vanished in all patients,the operative time was (42.5 ± 15.7) min,the length of stay in hospital was (3.9 ± 0.6) d.No death and Horner syndrome occurred.All the patients were followed up for 6-24 months,compensatory hyperhidrosis was found in 26 patients,and no recurrence was found.Conclusion T3-4 thoracic sympathectomy is a safe and effective minimally invasive treatment for primary hyperhidrosis.
5.Anti-human immunodeficiency virus type 1 activity of two new nonnucleoside reverse transcriptase inhibitors in combination with other 3 antiretroviral agents in vitro
Dao-Min, ZHUANG ; Si-Yang, LIU ; Ru-Hua, DONG ; Li, BAI ; Biao, JIANG ; Xiao-Long, ZHAO ; Jing-Yun, LI
Bulletin of The Academy of Military Medical Sciences 2009;33(6):509-512
Objective:To evaluate the anti-HIV-1 activity of two new nonnucleoside reverse transcriptase inhibitors (NNRTIs), JB25 and JB26, in combination with 3 approved drugs (AZT, EFV, SQV)in vitro.Methods:The serially diluted 10 concentrations of JB25 and JB26 were combined with 7 serially diluted AZT, EFV and SQV respectively.The combination was added to 384 cell culture plates and then cocultured with HIV-1 ⅢB infected MT-2 cells for 3 days. Finally, the HIV-1 production was determined by measuring the expression of reporter genes of TZM bl cells. The data were analyzed by MacSynergy Ⅱ software.Results:The average capacity of synergism/antagonism of JB25 with AZT, EFV and SQV was 244.45/-5.05(nmol/L)~2%, 119.58/-65.93 (nmol/L)~2% and 145.83/-0.32 (nmol/L)~2% respectively;the average capacity of synergism/antagonism of JB26 with AZT, EFV and SQV was 398.90/0(nmol/L)~2%, 103.62/-0.49(nmol/L)~2% and 138.473/-0.27 (nmol/L)~2% respectively. Conclusion:Two new NNRTIs JB25 and JB26 develop synergism when combined with 3 approved drugs, respectively. MacSynergy Ⅱ software could evaluate the anti-HIV-1 activity of drug combination.
6.Study on chemical constituents from seed of Oroxylum indicum.
Xiang-yu ZHAI ; Wei XIAO ; Biao YANG ; Zhao-qing MENG ; Zhen-zhong WANG ; Wen-zhe HUANG ; Kai-jin WANG
China Journal of Chinese Materia Medica 2015;40(15):3013-3016
Oroxylum indicum was a traditional Chinese medicine. In order to study the chemical constituents from the seed of O. indicum, the chemical constituents of 80% methanol extract of seeds of O. indicum were subjected to chromatography on silica gel, Sephadex LH-20, and preparative HPLC, leading to the isolation of eleven compounds. The structures were identified by various spectroscopic data including ESI-MS, 1H-NMR and 13C-NMR data as oroxin B (1), chrysin (2), baicalein (3), neglectein (4), quercetin-3-O-β-D-galactopy ranoside (5), quercetin-7-O-β-D-glucopyranoside (6), 2α,3β-dihydroxylluPeol (7), lupeol (8), rengyol (9), β-sitostero (10), and stigmasterol (11). Among them, compound 5 were firstly obtained from O. indicum.
Bignoniaceae
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chemistry
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Magnetic Resonance Spectroscopy
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Seeds
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chemistry
7.Protective effect of lycopene on human spermatozoa during cryopreservation and its mechanism.
Zuo-wen LIANG ; Kai-min GUO ; Xiao-fan DAI ; Ling-yun LIU ; Sheng-qi XU ; Li-jing ZHAO ; Fu-biao LI ; Hong-liang WANG
National Journal of Andrology 2015;21(6):521-526
OBJECTIVETo investigate the protective effect of lycopene against cryopreservation injury of post-thawing human sperm and its mechanism.
METHODSSemen samples were collected from 25 volunteers, each sample equally divided into four parts to be cryopreserved with cryoprotectant only (Ly0 control) or cryoprotectant + lycopene at the concentrations of 2 (Ly2), 5 (Ly5), and 10 µmol/L (Ly10), respectively. Before and after thawing, the semen samples were subjected to computer-assisted semen analysis ( CASA) for sperm kinematics, flow cytometry for sperm apoptosis, thiobarbituric acid assay for malondialdehyde (MDA) concentration, and JC-1 fluorescent staining for the sperm mitochondrial membrane potential (MMP).
RESULTSAfter cryopreservation, sperm motility was markedly decreased in all the groups (P < 0.01). The rate of sperm apoptosis was significantly lower in the Ly5 group than in the Ly0 control ([25.68 ± 4.36]% vs [33.26 ± 4.78]%, P < 0.05), while sperm MMP remarkably higher in the former than in the latter ([66.18 ± 14.23]% vs [55.24 ± 12.31]%, P < 0.05). The Ly2, Ly5 and Ly10 groups showed no statistically significance differences in the MDA level from the Ly0 control (P > 0.05).
CONCLUSIONAddition of lycopene at a proper concentration to cryoprotectant may reduce oxidative damage to sperm mitochondria in the freezing-thawing process, attenuate oxidative stress injury induced by reactive oxygen species to sperm plasma membrane, and improve the anti-apoptosis ability of sperm.
Apoptosis ; Carotenoids ; pharmacology ; Cryopreservation ; Cryoprotective Agents ; pharmacology ; Flow Cytometry ; Humans ; Male ; Malondialdehyde ; analysis ; Oxidative Stress ; Reactive Oxygen Species ; Semen Analysis ; Semen Preservation ; adverse effects ; methods ; Sperm Motility ; Spermatozoa ; drug effects ; physiology
8.Effects of competitive and noncompetitive 5α-reductase inhibitors on serum and intra-prostatic androgens in beagle dogs.
Xiao-feng ZHAO ; Yong YANG ; Wei WANG ; Zhi QIU ; Peng ZHANG ; Biao WANG
Chinese Medical Journal 2013;126(4):711-715
BACKGROUND5α-Reductase inhibitors (5α-RI) act by inhibiting the conversion of testosterone to dihydrotestosterone (DHT), thereby preventing DHT induced benign prostatic hyperplasia. The existing 5α-RIs can be classified into two types: competitive and noncompetitive. Currently, limited evidence is available concerning the effect differences between the two types of 5α-RI on androgens. The purpose of this study was to assess the effects of competitive and noncompetitive 5α-RIs on serum and intra-prostatic androgens in beagle dogs.
METHODSTwenty beagles with spontaneous benign prostatic hyperplasia were randomly allocated into two groups: epristeride group (n = 10) in which beagles were treated with epristeride at 1 mg/kg once a day for 3 months, and finasteride group (n = 10) in which beagles were treated with finasteride at 1 mg/kg once a day for 3 months. The levels of intra-prostatic testosterone and DHT were measured before treatment and on day one after three months medication. Serum levels of testosterone and DHT were measured at the same time points. Changes in androgen levels before and after treatment were analyzed, and comparisons were made within each treatment group and between treatment groups.
RESULTSAfter 3-month treatment, serum and intra-prostatic DHT levels all decreased significantly in both the epristeride and finasteride groups. The change of DHT in serum was significantly higher in the finasteride group (-14% and -43% in epristeride and finasteride groups respectively, with P < 0.001); however there was no significant difference in the changes of intra-prostatic DHT between the two groups (-47% and -51% in epristeride and finasteride groups, respectively, P = 0.304). The decreases in DHT levels were accompanied by reciprocal increases in serum and intra-prostatic testosterone levels. Changes of testosterone were significantly higher in finasteride group both in serum (20% and 42% in epristeride and finasteride groups, respectively, P < 0.001) and in prostate tissue (18% and 29% in epristeride and finasteride groups, respectively, P = 0.004).
CONCLUSIONSTwo types of 5α-RI have similar effects in reducing DHT in prostate tissue in beagles. Competitive 5α-RI may reduce serum DHT to a greater scale, and significantly increase testosterone in beagle serum and prostate.
5-alpha Reductase Inhibitors ; pharmacology ; Androgens ; blood ; metabolism ; Androstadienes ; pharmacology ; Animals ; Dihydrotestosterone ; blood ; metabolism ; Dogs ; Finasteride ; pharmacology ; Male ; Prostate ; drug effects ; metabolism
9.'Life Satisfaction' as a moderator or mediator of accommodation category and loneliness for rural school students
Zhao-Hui HUANG ; Fang-Biao TAO ; Jia-Hu HAO ; Ling YANG ; Dai-Juan CHENG ; Li-Min XIAO
Chinese Journal of Epidemiology 2009;30(3):222-226
Objective To examine life satisfaction as a moderator or mediator of accommodation category and loneliness for elementary and middle school students in rural areas so as to provide evidence for psychological health intervention among said students. Methods All participants were asked to complete an anonymous questionnaire battery which including an 18-item General Health Questionnaire, Children' s Loneliness Scale and Multidimensional Students' Life Satisfaction. Results The average score of loneliness scale (35.0±9.5) among boarding-school students was significantly higher than those students living at home (33.1±10.1 ) (P<0.01). The average scores on school-satisfaction (33.5±6.3), friend-satisfaction (42.1±6.2) and self-satisfaction (32.4±5.0) of boarding-school students were significantly higher than those students living at home (35.9±6.6, 42.9±6.6,32.9±5.3 ) ( P< 0.05 ). The association between accommodation category and loneliness was fully mediated by school-satisfaction (the standardized coefficients of loneliness was reduced from 0.043 (P<0.05) to 0.021 (P>0.05) and partly mediated by self-satisfaction and friend-satisfaction. Data through Moderation analyses indicated that self-satisfaction, school-satisfaction and friend-satisfaction did not serve as moderators. Conclusion Accommodation category, life satisfaction seemed to be good predictors on loneliness among elementary and middle school students and the fully mediated effect of school-satisfaction between accommodation category and loneliness was significant, suggesting that intervention of loneliness should focus on these variables.
10.An investigation on the epidemic situation of intimate partner violence in 2575 college students
Pu-Yu SU ; Jia-Hu HAO ; Zhao-Hui HUANG ; Li-Min XIAO ; Fang-Biao TAO
Chinese Journal of Epidemiology 2011;32(4):346-351
Objective To investigate the prevalence of love affairs and intimate partner violence,and to explore the relationship between intimate partner violence and other mental health and risk behavior in college students.Methods Three universities were selected using cluster sampling method in Hefei and Wuhu.Totally.2575 college students completed an anonymous questionnaire.Intimate partner violence,depression,satisfaction of school life,self-esteem,suicidal psychology and behavior were evaluated to estimate the relationship between intimate partner violence and mental health/risk behavior.Results There were 46.9%students reported that they had intimate partner currently or in the past.The rate of having intimate partner in male students was higher than that in female students(x2=44.13,P<0.001).And the rates were higher in sophomores and juniors than in freshmen(x2=161.84,P<0.05).There were 21.1% students had sexual behavior with their intimate partners.But only 21.8%(34/156)intimate partners reported that they used condom every time.There were 11.5%(18/156)intimate partners reported that they never took any contraception.There were 18.6%(29/156)students reported that they were pregnant or led to their girlfriend becoming pregnant,but only less than 50.0% adolescents induced abortion in a legal hospitals.The rates of being the victim of physical assault,emotional abuse,sexual coercion,the total intimate partner violence were 18.0%,33.6%,5.1%.37.1%.The rates of being the victim of physical assault,emotional abuse,total intimate partner violence in male adolescents were higher than those in female adolescents,but the rate of sexual coercion was on the contrary(x2=70.21,13.25,14.04,5.77,P<0.05).Among the intimate partners who had suffered from intimate partner,74.2%(345/446)students had underwent more than 3 times.and 47.1% had underwent more than 2 types of intimate partner violence.The score of depression was highest in the victims of intimate partner violence,but the scores of self-esteem and school life satisfaction were on the contrary(F=4.00,16.39,8.76,P<0.05).The rates of suicidal ideation,suicidal plan,suicidal preparative and attempted suicide were highest in the victims of intimate partner violence(x2=13.80,9.72,8.52,11.96,P<0.05).Conclusion The rate of having sexual behavior with their intimate partners was high in college students,but their self-protection awareness was insufficient.In the present study,a high prevalence of intimate partner violence among university students was observed,and highlighted a need for attention to the other mental health and risk behavior in adolescents with intimate partner violence.