Objective To investigate the effect of glutaminase 1(GLS1)specific inhibitor BPTES[bis-2-（5-phenylacetamido-1,3,4-thiadiazol-2-yl）ethyl sulfide]on the liver fibrosis in the mouse model of liver fibrosis induced by carbon tetrachloride(CCl4).Methods Male C57BL/6J mice were intraperitoneally injected with olive oil(control group),10%CCl4(10 μL/g,model group)or 10% CCl4(10 μL/g)+ BPTES(10 mg/kg,treatment group),with 10 mice in each group,two doses a week for four weeks to establish liver fibrosis model. Collagen deposition in mouse liver tissue was observed by Sirius red staining. The expression levels of actin alpha 2(Acta2),collagen typeⅠalpha 1(Col1a1)GLS1 and GLS1 protein were detected by qRT-PCR and immunohistochemical staining.Results Compared with the control group,the liver tissue of mice in the model group was generally enlarged,the surface was not smooth and granular,and the ratio of liver mass to tibia length significantly increased(t = 2. 979,P < 0. 05);The Sirius red positive area of collagen deposition increased signifi-cantly in the liver tissue of mice in the model group(t = 7. 661,P < 0. 01),the relative expression levels of Acta2 and Col1a1 significantly increased(t = 4. 335 and 5. 319,respectively,each P < 0. 01),and the mRNA and protein levels of GLS1 significantly increased(t = 5. 319 and 9. 725,respectively,each P < 0. 01). However,compared with the model group,the BPTES treatment group had a reduction in liver mass,a significant reduction in the Sirius red positive area of collagen deposition in liver tissue(t = 7. 427,P < 0. 01),and a significant reduction in the relative expressions of Atca2 and Col1a1(t = 3. 713 and 2. 628,respectively,each P < 0. 05).Conclusion Inhibition of GLS1activity can significantly improve the degree of liver fibrosis induced by CCl4,providing a new idea for the treatment of liver fibrosis.

Humans ; Penicillins ; adverse effects ; immunology ; therapeutic use ; Skin Tests ; classification ; methods ; standards

China ; Hospital Design and Construction ; standards ; Hospitals ; Humans ; Occupational Exposure ; analysis ; prevention & control ; Radiology Department, Hospital ; Safety ; standards

Animals ; Bronchopulmonary Dysplasia ; etiology ; Fibroblasts ; physiology ; Humans ; Hyperoxia ; pathology ; Infant, Newborn ; Lung ; embryology ; pathology

AIM: To approach the best therapeutic measures after allochthonous intermingled with autogenous bone transplantation in children with tumor-like legion. METHODS: Forty children received bone transplantation between from March 2003 to March 2006 were selected from First Hospital Affiliated to Zhengzhou University. The guardians of Children all knew and agreed with the items. Subjects were divided into two groups according to the bone grafts with 20 children in each group: Compound transplantation group and autoplastic transplantation group. ① Subjects in compound transplantation group were transplanted with compound bone of allochthonous bone, autogenous bone and marrow with 160 thousands of gentamicin, 400 mg of vacocin and 5 mg of dexamethasone added. Allochthonous bone was obtained from dried radiosteriliaed bone, which was made by Shanxi Aorui Biomaterial Co., Ltd. Autogenous bone was gotten from the iliac bone of children. ② Subjects in autoplastic transplantation group were transplanted with simple illac bone got from subjects. Meanwhile, 16 thousands of gentamycin was adopted in the transplantation without using dexamethasone. ③ 5 mg of dexamethasone was added in to the allochthonous bone used for the surgery of subjects in compound transplantation group, and intervenous drop infusion of hormone was given to subjects after the surgery at proper dose, while children in autoplastic transplantation group received no hormone during and after the surgery. ④ Post-operation draining was performed, and the draining flow within 24 hours less than 20 mL was taken as the index of extubate. ⑤ The allochthonous bone and autoplastic bone were immersed into 160 thousands of gentamicin and 400 mg of norvancomycin used for patients in the compound transplantation group. Patients in both groups were administrated with antibiotic drugs of blue mold for fear of infection. ⑥ The duration of recovery to normal body temperature, leukocyte count and erythrocyte sedimentation rate (ESR) as well as the time of reservation of drainage tube of patients in both groups were observed after the surgery after operation, and follow-up was conducted regularly. RESULTS: Totally 40 patients were involved in the analysis of results, and no one withdrew from the study. ① The duration of recovery to normal body temperature, leukocyte count and ESR as well as the time of reservation of drainage tube of patients in compound transplantation group were longer than those in autoplastic group [(10.95?2.63,13.85?2.68,25.90?2.53,13.15?3.01) days;(6.95?1.57,8.80?1.96,15.30?2.27,6.40?1.98) days,P

Objective To observe the effectiveness of botulinum toxin A (BTXA) in the treatment of small sample patients with acute acquired concomitant esotropia. Design Retrospective case series. Participants 6 patients (3 female and 3 male) with acute acquired concomitant esotropia aged 6 to 34 years. Methods All the patients were received 2.5 units of BTXA injected into the bilateral medial rectus muscles once under electromyographic control. They were performed alter prism-cover test,synoptophore and stereoscopic charts test. Main Outcome Measures The alignment of eyes and binocular vision. Results The follow-up of patients was 4 to 39 months. The pre-injection angle of deviation was 43.3?1.0△; the angle of deviation at the last follow-up was 3.3?8.2△,and 5 patients achieved alignment. In pre-injection,one patient had distance stereopsis,2 patients had near stereopsis with 600 seconds and 40 seconds; after injection,5 patients demonstrated distance and near stereeopsis (3 patients 40 seconds,1 patient 60 seconds,and 1 patient 200 seconds). Conclusion This small sample study shows that BTXA injection is effective alternative in treating acute acquired concomitant esotropia.

Objective To create a sterilized condition in field operation and the daily grind avoiding the intercross contamination between the experimentation and outside. Methods The air in the experimentation was filtrated by primary and advanced filter. High intense ultraviolet was used to sterilize the air, and negative compression was kept in the experiment condition. Results Hyperpurificatory biosafety cabinet guaranteed the sterilization condition in field operation and the daily grind, avoiding the intercross contamination. Conclusion Hyperpurificatory biosafety cabinet can be used in medical treatment, pharmacy, food industry, electrical industry, instrumentation, bioengineering, scientific tests, especially biology species identification in biological warfare.

According to the medical support missions of our hospital,a new management mode for medical support equipment is proposed including both warehouse management and daily supervision. This kind of management bases itself on peace time and focuses on war time.

Objective:To study the role of adenoviral-mediated PTEN gene transfection in reversing drug resistance of trastuzumab-resistant human breast cancer.Methods:Recombinant adenoviruses carrying PTEN gene(AdPTEN) were constructed and transfected into trastuzumab-resistant human breast cancer cell line BT474.Proliferation and apoptosis of BT474 cells treated with AdPTEN and trastuzumab were measured by MTT assay and FCM.Time course of BT474 cells apoptosis induced by AdPTEN was analyzed by detection of DNA fragmentation.Western blotting was employed to measure phosphorylated-Akt expression levels in AdPTEN treated BT474 cells.The nude mice model of BT474 cells was employed to observe the effects of AdPTEN and trastuzumab on trastuzumab-resistant human breast cancer in vivo.The expression of PTEN gene,cancer cells apoptosis and ultrastructural changes were evaluated after the mice were sacrificed.Results:We successfully constructed recombinant AdPTEN;and the titer of the recombinant adenovirus was about 4.2?1011TCID50/ml.PTEN gene expression was identified by PCR,RT-PCR and Western blotting assay.Combinatorial AdPTEN and trastuzumab significantly suppressed the proliferation of BT474 cells and induced the apoptosis.The percentage of apopotosis cells treated with AdPTEN was(20.7?5.82)%,companied by cells cycle arrest in G1 phrase(P

Objective To research a more effective method of full-text retrieval of electronic care record (ECR), which avails clinical work, medical research and ECR management. Methods Based on full -text retrieval by keyword, by changing the form of ECR with XML,a full-text retrieval system of ECR based on Lucene was built. Results The system testing showed that the precision ratio of ECR retrieval was raised. Conclusion The full-text retrieval system of ECR meets the design requirements and the function needs.