1.Effects of air pollution on lung function and nasal inflammatory factors in healthy migrant college students
FANG Xue ; ZHANG Qianye ; XIAO Sha ; XUE Ping
China Tropical Medicine 2024;24(4):417-
Abstract: Objective To understand the current situation of lung function and nasal inflammation of students from different places of origin, and to explore the possible relationship between air pollutants and lung function and inflammatory factors of healthy college students. Methods A self-designed questionnaire was used to investigate 253 healthy college students, including their basic personal information, the origin of students, and their living environment, then their lung functions were tested, including forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), and peak expiratory flow (PEF). Furthermore, according to the air quality of the region of origin, 60 students from areas with good air quality and 30 students from areas with poor air quality were selected. Nasal inflammatory factors (IL-6 and TNF-α levels) were detected before (July 12, 2019) and after summer vacation (August 27, 2019) to analyze the impact factors on lung function in healthy college students and the level of nasal inflammatory markers after short-term differential exposure to air pollutants. Results The univariate analysis of the questionnaire survey showed that the lung function of healthy college students was influenced by urban-rural distribution, air quality of students' origin, and height. Multivariate analysis found that compared with urban healthy college students, the FVC and FEV1 of rural students increased by 0.346 L and 0.322 L respectively (P<0.05). In addition, for each decrease in the grade of air quality of students' origin, FEV1 and PEF decreased by 0.193 L and 0.687 L/s respectively (P<0.05). After differential exposure to air pollutants during the holiday, healthy college students from areas with poor air quality had higher levels of the nasal mucous membrane inflammatory marker TNF-α than those from areas with good air quality (P<0.05). Conclusions The lung function of healthy college students is affected by urban-rural distribution and the air quality of their region of origin, and the short-term differential exposure to air pollutants leads to changes of nasal inflammatory factors in young healthy individuals.
3.Extranodal follicular dendritic cell sarcoma of neck region: report of a case.
Chinese Journal of Pathology 2012;41(6):410-411
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Carcinoma
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metabolism
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pathology
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Cyclophosphamide
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therapeutic use
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Dendritic Cell Sarcoma, Follicular
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drug therapy
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Doxorubicin
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therapeutic use
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Head and Neck Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
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Humans
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Lung Neoplasms
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drug therapy
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secondary
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Male
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Melanoma
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metabolism
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pathology
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Middle Aged
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Neoplasm Recurrence, Local
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Prednisone
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therapeutic use
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Receptors, Complement 3b
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metabolism
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Receptors, Complement 3d
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metabolism
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Vincristine
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therapeutic use
4.Study of the common genotype of glucose-6-phosphate dehydrogenase deficiency in newborn infant in Guangdong region
Xue XIAO ; Youxiang ZHANG ; Li YU ; Yao ZHANG
Chinese Journal of Applied Clinical Pediatrics 2014;29(8):616-618
Objective To investigate the genotypic frequency of glucose-6-phosphate dehydrogenase (G6PD) (1376G > T),G6PD 1311C > T and G6PD IVS11-93T > C in 50 newborns with G6PD deficiency in Guangdong region.Methods To identify G6PD deficiency in the patients of neonatal ward in Guangzhou First People's Hospital during 2010,detected by methemoglobin reduction test and measurement of G6PD/6-phosphate dehydrogenase (6PGD) ratio.Fifty G6PD deficiency subjects were classified into the experimental group,20 neonatal jaundice subjects were classified into the control group.Genomic DNA was extracted by standard method from the peripheral blood of each subject.PCR-direct DNA sequence analysis was used to identify G6PD 1376G > T,1311C > T and 11 intron 93T > C mutations.Results The overall results of mutation analysis in the 50 G6PD deficiency subjects showed the existence of 3 different alleles:G6PD 1376G >T,1311C >T,11 intron 93T > C(including 1311C > T/IVS11-93T > C and 1376G >T/1311C >T/IVS11-93T > C complex mutations).The different genotypic frequency in the experimental group was G6PD 1376G >T 26.0% (13/50 cases),1311C > T 4.0% (2/50 cases),11 intron 93T > C 4.0% (2/50 cases),1311C > T/IVS11-93T > C 2.0% (1/50 cases),1376G > T/1311C > T/IVS11-93T > C 2.0% (1/50 cases).The G6PD enzyme activity of the subjects with 1311C > T/IVS11-93T > C and 1376G > T/1311C > T/IVS11-93T > C complex mutation were reduced.These G6PD mutations were not found in the controls.Conclusions G6PD 1376G > T is one of the commonest mutation in G6PD deficiency newborn in Guangdong region.A novel complex mutation 1376G > T/1311C > T/IVS11-93T > C in this study was found.
5.Effect of dexmedetomidine on acute kidney injury after cardiac valve replacement with cardiopulmonary bypass
Feng XUE ; Wei ZHANG ; Xiao ZHANG ; Yan JIANG ; Haichen CHU
Chinese Journal of Anesthesiology 2016;36(10):1171-1174
Objective To evaluate the effect of dexmedetomidine on acute kidney injury after cardiac valve replacement with cardiopulmonary bypass (CPB).Methods One hundred patients of both sexes with rheumatic heart disease,aged 32-64 yr,weighing 46-75 kg,of American Society of Anesthesiologists physical status Ⅱ or Ⅲ (New York Heart Association class Ⅱ or Ⅲ),scheduled for elective cardiac valve replacement with CPB,were divided into 2 groups (n =50 each) using a random number table:control group (group C) and dexmedetomidine group (group D).Dexmedetomidine was intravenously infused in a loading dose of 1 μg/kg over 10 min before induction of anesthesia followed by an infusion of 0.4 μg · kg-1 · h-1 until 24 h after operation in group D,while the equal volume of normal saline was given in group C.The urine output per hour during the postoperative 48 h period was recorded.At 6,12,24,36 and 48 h after operation,blood samples were collected from the median cubital vein for determination of serum creatinine levels.The development and severity of acute kidney injury were determined according to the urine output and serum creatinine level.Results Compared with group C,the incidence and severity of acute kidney injury were significantly decreased in the postoperative 48 h period in group D (P<0.05).Conclusion Dexmedetomidine infused in a loading dose of 1 μg/kg over 10 min before induction of anesthesia followed by an infusion of 0.4 μg · kg-1 · h-1 until 24 h after operation can reduce the development and severity of acute kidney injury after cardiac valve replacement with CPB in patients.
6.Effects of Therapist Training:Evaluated with Kirkpatrick's Model
Xue WANG ; Ping ZHU ; Shuyan ZHANG ; Feng JIN ; Dunwu XIAO
Chinese Journal of Rehabilitation Theory and Practice 2016;22(9):1114-1116
Objective To evaluate the effect of training for therapists based on Kirkpatrick's Four-level Model. Methods Totally 39 ther-apists who completed 2014 Beijing Clinical Shortage of Manpower Training Programs (Therapist) were investigated with Kirkpatrick's Four-level Model, named reaction, learning, behavior and results. Results For reaction level, the total satisfaction was high, but different with the degree of education, especially in the items of Participate in Training, Type of Cases, Organizing on Practice Training, Organizing Case Discussions and Get A Lot of Benefits from the Training (F>4.59, P<0.05). For learning level, the test scores were significantly differ-ent before and after the training (t>9.53, P<0.001). For behavior level, the self-reports of the therapists, and the reports from their depart-ment directors and colleagues all agreed that their ability improved after training (t>4.44, P<0.001). For results level, eleven therapists passed the rehabilitation therapist test, two therapists participated in some researches, and one published an article. Conclusion The thera-pists satisfied in the training, that may improve the ability of therapists to contribute to their organization.
7.Role of NF-?B in the mechanism of liver injury following intestinal perforations due to abdominal firearm wound in pigs
Zexin LI ; Xiao WANG ; Huichao XUE ; Jiangwei LIU ; Yongjiu ZHANG
Chinese Journal of Current Advances in General Surgery 2009;0(08):-
Objective:To study the role of NF- ?B in the mechanism of liver injury following intestinal perforations due to abdominal firearm wound. Methods:A total of 42 Chang-Bai piglets were assigned randomly into 7 groups: control group and wounded 1, 2, 4, 8, 12, 24 hours group.The model of intestinal perforations due to abdominal firearm wound was established in wounded groups. Hepatic NF-?B and TNF-? content was measured with immunohistochemical staining and image analysis in all groups. Hepatocyte apoptosis indexes and serum ALT levels were also determined at the same time. The alterations of hepatic tissue were observed under light microscope. Results: Levels of hepatic NF-?B activity in wounded groups were significantly elevated compared with control group, and two peaks appeared in 1 h group and 8 h group, respectively (P
8.Role of TNF-? in the mechanism of hepatocyte apoptosis induced by intestinal perforations due to abdominal firearm wound
Zexin LI ; Xiao WANG ; Huichao XUE ; Jiangwei LIU ; Yongjiu ZHANG
Chinese Journal of Current Advances in General Surgery 2009;0(12):-
Objective:To study the role of TNF-?in the mechanism of hepatocyte apoptosis induced by intestinal perforations due to abdominal firearm wound. Methods:A total of 42 Chang-Bai piglets were divided randomly into 7 groups: control group and wounded 1, 2 , 4, 8, 12, 24 hour groups.The model of intestinal perforations due to abdominal firearm wound were established in wounded groups. Levels of plasma endotoxin were measured using ehromogenic limulus amehoeyte lysate test.Hepatic TNF-? content was measured with immunohistochemical staining and image analysis in all groups. Hepatocyte apoptosis indexes and serum TNF-? levels were determined at the same time. Results: Levels of plasma endotoxin, serum TNF-?, hepatic TNF-? content and hepatocyte apoptosis indexes in wounded groups were all significantly elevated compared with control group(P
9.Expression of DNA methyltransferase 1 mRNA in patients of endemic arsenism and its clinical significances
Xue-li, PAN ; Ai-hua, ZHANG ; Xiao-xin, HUANG
Chinese Journal of Endemiology 2010;29(1):13-17
Objective To investigate the transcription and expression of DNA methyltransferase 1 (DNMT1) mRNA in endemic arsenism patients by burning coal usage,to probe its effects on the development and carcinogenesis of arsenism. Methods In 2008,68 arsenism patients(including 24 mild cases,28 moderate cases and 16 severe cases) were selected in the areas with endemic arsenism according to Standarding of Diagnosis for Endemic Arsenism from Xingren county,Guizhou province. Among the subjects,40 cases were diagnosed by pathological methods,and they were divided into general pathological changes(20),precancerous(14) and cancerous group(6). Tweleve kilometer away from the endemic arsenism area,23 controls were selected in Daguoduo village (non-arsenism exposure). Under the principle of informed consent,blood samples were collected from individuals. The mRNA expression of DNMTI was detected by real-time quantitative reverse transcription polymerase chain reaction(FQ-PCR). At the same time,skin tissue samples were collected from the voluntary surgical treatment patients with endemic arsenism (total 61 cases,including 34 general pathological changes cases,21 precancerous cases and 6 cancerous cases) and from the control(15 cases). DNMT1 protein was detected by immunohistochemical method.Results Average level of DNMT1 mRNA were 0.221 83±0.595 09,0.246 11±0.509 79 and 0.389 27±0.411 33 respectively among mild,moderate and severe arsenism group. DNMT1 mRNA level of mild and moderate group were obviously lower than the control group(0.695 95±0.463 98,all P < 0.01). The mRNA average level of DNMT1 were 0.320 64±0.547 46,0.313 09±0.529 13 and 0.159 07±0.342 56 individually among general pathological changes,precancerous and cancerous group,which were obviously lower than the control group(0.695 95±0.463 98,all P < 0.05). The expression rates of DNMT1 protein in skin were 88.24%(30/34),100%(21/21) and 100% (6/6) among general pathological changes,precancerous and cancerous group were higher than the control group [0(0/15),all P < 0.01],and the extent of expression gradually increased with the aggravation of skin damage(r,= 0.740,P < 0.01). Conclusions DNMT1 participated in the development of the arsenism. High expression of its protein was an early event during the process of the arsenism. DNMT1 may be the new target markers for early diagnosis and treatment of arsenism.
10.An in vitro analytical method based on bio-thermal activity for the determination of dissolution rate of Chinese medicine solid preparation.
Xue HUANG ; Hailong YUAN ; Xiaohe XIAO ; Tiantian ZHANG
Acta Pharmaceutica Sinica 2010;45(3):338-42
To explore the new pattern of Chinese medicine solid preparations (CMSP) in vitro dissolution, a method testing the bio-thermal activity in combination with UPLC was used. Microcalorimetry was used to obtain the characteristic metabolic growth power-time curves and a series of biothermodynamic parameters of the inhibition of Staphylococcus aureus by Yinhuang tablet dissolving solutions at the pH 6.8 (phosphate buffer) dissolution medium at different times. From these results, the cumulative dissolution of Yinhuang tablet based on bio-thermal activity was obtained. The dissolution rates of two components of chlorogenic acid and baicalin were determined by UPLC method. Then f2 similar factor method was used to evaluate the relevance of these two methods. The result showed that f2 values all were more than 50, indicating that there is a good correlation between the two methods of measuring the dissolution rate. It is feasible to determine CMSP in vitro dissolution by using bio-thermal activity, and to provide new evaluation methods for controlling the quality of CMSP.