1.Effects of genistein on expressions of jak1 kinase and inteleukin-4 in lung of guinea pigs with bronchial asthma.
Xiu-feng ZHANG ; Zhen-hua HE ; Xiao-wu TAN
Chinese Journal of Applied Physiology 2009;25(3):328-348
Animals
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Asthma
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drug therapy
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metabolism
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Genistein
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pharmacology
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therapeutic use
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Guinea Pigs
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Interleukin-4
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genetics
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metabolism
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Janus Kinase 1
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genetics
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metabolism
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Lung
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metabolism
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Male
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Protein Kinase Inhibitors
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pharmacology
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therapeutic use
2.The expression and significance of SSX gene in primary liver carcinoma
Yanan ZHEN ; Ruixue XIAO ; Weixia NONG ; Qiong SONG ; Shaojian HE
Chinese Journal of Hepatobiliary Surgery 2015;21(2):105-108
Objective To detect the expression of SSX and to correlate it with clinical indicators of primary hepatocellular carcinoma (HCC).Methods The expression of SSX1-5 mRNA and SSX1 protein were respectively detected by RT-PCR and Western blot and immunohistochemistry staining.The relation between the expression of SSX mRNA and SSX1 protein with clinical indicators were analysed.Results SSX1,SSX2,and SSX3 mRNA were expressed in hepatocellular carcinoma cell lines BEL-7404,Hep G2,and SMMC-7721.In 26 HCC samples,SSX1-SSX5 mRNA was detectable in 53.8%,42.3%,50.0%,46.2% and 26.9%.The expression of SSX1 mRNA was not related to serum AFP levels (P >0.05).Specific expression was both found in the normal group and the high value group.The expression rate of SSX1 mRNA was 85.7% in the older group,which was higher than in the younger group (16.7%,P < 0.05).The expression rate of SSX1 protein was 50% in HCC tissues,which was not seen in the caner-adjacent or cirrhosis tissues.In 49 HCC paraffin tissue section samples,the expression rate of SSX1 protein was higher than that in caner-adjacent tissues (46.9% vs 18.4%,P < 0.05).The expression rate of SSX1 protein was 68.3% in the large hepatocellular carcinoma group,which was higher than in the small hepatocellular carcinoma group (29.6%),(P < 0.05).Conclusions SSX1 mRNA is expressed with a high percentage and specificity in HCC and their products are new potential promising targets for antigen-specific immunotherapy of HCC.The detection of SSX1 expression has the potential value for auxiliary diagnosis of HCC.
3.Effect of FTY720 inhibiting corneal neovascularition induced by sphingosine 1-phosphate
Fan, ZHONG ; Xiao-Zhen, DING ; Wei-Zhong, YANG ; Zong-Yin, GAO ; Xiao-He, LU
International Eye Science 2015;(7):1134-1138
AlM: To explore the inhibiting effect of FTY720 on corneal neovascularization ( CNV) of rat.METHODS: MTT assay and cells scratch were adopted to observe hyperplasia of human umbilical vein endothelial cells ( HUVECs ) and cell migration induced by sphingosine-1-phosphate(S1P) after using FTY720 of different concentration. The effect of FTY720 on CNV induced by S1P in a rat corneal micropocket model was detected. 30SD rats were randomly divided into group A, group B and group C with 10 rats per group. S1P and 0μg, 5μg, and 20μg FTY720 controlled-released particles were implanted into the corneal stroma. The growth of CNV and having pathological examination on 12d after the operation was observed. Findings was analyzed by one-way ANOVA.RESULTS: 10, 102 , 103 , and 104 nmol/L FTY720 and HUVECs co-incubate 72h could inhibit cell proliferation (P < 0. 01 ), 24h after the function of 10, 100nmol/L FTY720, it could inhibit S1P-induced cell migration and the ability of restricting cell proliferation and cell migration was enhanced with increasing concentration of FTY720. On 12d, after rat corneal micropocket controlled-release particles was implanted into groups A, B, C, the CNV area were respectively 10. 05±1. 19, 6. 59±0. 95, 2. 70± 0.68mm2(F=145. 155, P<0. 01), group A and group B was statistically different and this was the same case between group B and group C (P<0. 01).CONCLUSlON:FTY720 can inhibit S1P-induced corneal neovascularization.
4.The correlation between Val66Met polymorphism in BDNF gene and Alzheimer's disease
Xiao-Ming HE ; Zhen-Xin ZHANG ; Jun-Wu ZHANG ; Yong-Tao ZHOU ; Mou-Ni TANG ; Cheng-Bin WU ; Zhen HONG ;
Chinese Journal of Geriatrics 1995;0(02):-
0.05).Our data also showed no significant association between the genotypes and the severity of the disease.One-way ANOVA showed that BDNF genotype had no association to the age of onset for developing AD.Conclusions Our results indicate that Va166Met SNP in BDNF gene is not associated with AD.
5.Analysis of 105 cases of pregnancy -induced hypertension patients with Ⅲphase of retinopathy
Xiao-Yun, QIU ; Jian-Ling, GUO ; Feng-Ci, YU ; Shu-Zhen, LIANG ; Zhen-An, LI ; Qing-Quan, HE
International Eye Science 2014;(9):1699-1701
To explore the related risk factors and outcome in pregnancy- induced hypertension patients with Ⅲ phase of retinopathy.
●METHODS: A total of 105 pregnancy - induced hypertension patients with Ⅲ phase of retinopathy in our hospital from Januany 2012 to December 2013 were enrolled. Clinical date of them were collected to analyze.
●RESULTS: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy were positively correlated with the course of the disease, blood pressure, proteinuria, and it was higher occurred in cold winter and spring, timely termination of pregnancy and appropriate hormone therapy can promote the recovery of vision, and improve outcomes of pregnancy.
●CONCLUSlON: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy associated with season and disease severity. Timely treatment can restore normal vision, improve maternal and neonatal prognosis. Routine examination of fundus examination should be used as the pregnancy induced hypertension syndrome.
6.Expressions of Let-7 in Normal Breast Tissue and Breast Cancer Tissue
Zhen YU ; Mingyao XIAO ; Ming CUI ; Min LIU ; Shuying DAI ; Ying CHEN ; Yuefeng HE
Journal of Kunming Medical University 2016;37(7):19-21
Objective To study the expression characteristics of Let-7 genes in breast cancer.Methods Twenty-eight patients with breast cancer were randomly selected,and their cancer tissue and adjacent normal tissue were collected.TRIzol was used to extract the total RNA and real-time quantitative PCR was used to detect the relative gene expression levels.Results The expression of precursor Let-7 in cancer tissue was (9.65 ± 2.31),which was lower than that in normal tissue (10.05 ± 2.81),P =0.048.Precursor Let-7 had dependence relationship with the long menstruation (b =0.816,P =0.029).The menarche age showed a positive correlation with precursor Let-7 in normal tissue (r =0.502,P =0.048) and a negative correlation in cancer tissue (r =-0.484,P =0.049) Conclusions The expression of precursor Let-7 in cancer tissue is lower than that in normal tissue.The period of menstruation is a protective factor to breast cancer.
7.Single nucleotide polymorphisms, inflammation and nutrition of genes.
Acta Academiae Medicinae Sinicae 2005;27(6):790-792
Inflammation is part of the immune response, and inflammation may also induce or exaggerate some diseases through production of pro-inflammatory cytokines. More evidence have shown that the individual level of cytokine production is affected by single nucleotide polymorphisms in cytokine genes. Furthermore, as several nutrients participate in DNA protection and stabilization, altering gene expression and individual phenotype, nutrition has important interaction with inflammation. The purpose of this review is to give a recent update informations on the interaction of single nucleotide polymorphisms, inflammation and nutrition.
Fatty Acids
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pharmacology
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Fish Oils
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pharmacology
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Humans
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Inflammation
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genetics
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therapy
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Nutrition Therapy
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Polymorphism, Single Nucleotide
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drug effects
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Vitamins
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pharmacology
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Zinc
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pharmacology
8.Research Review of Functional Mechanism of Treatment by Way of Pasting TCM on Acupoints
Yanping HE ; Xiaoqin XIAO ; Guiming DENG ; Linqi OUYANG ; Zhen CHEN ; Zhiguo ZHANG
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(3):134-136
Treatment by way of pasting TCM on acupoints has a long history and the clinical efficacy is determined. In this article, based on the concept of TCM wholism and meridian theory, the mechanism of acupoint pasting therapy was expounded from the aspects of medicine effects on local stimulation of the body, meridian conduction, medicine transdermal absorption. Combining with the research hot spots of modern medicine transdermal delivery system, metabolomics, and efficacy material, this article proposed a new study mode of systems biology of TCM.
9.Primary adenosquamous carcinoma of liver: a clinical study of 2 patients
Wei DONG ; Lu LIU ; Jiannan HE ; Weidong XIAO ; Zhen WAN ; Yong LI
Chinese Journal of Hepatobiliary Surgery 2016;22(12):802-804
Objective To study the diagnosis and treatment of primary adenosquamous carcinoma (ASC) of liver.Methods The clinical and histopathological data of 2 patients with ASC were analyzed retrospectively.The cases were confirmed by surgery and histopathological examination.Results The tumors were located in the left liver in these 2 patients.Both patients presented with abdominal pain and they underwent surgical resection.On gross pathological examination,the tumors were irregular in shape with unclear boundaries.Microscopically,the tumor cells were arranged in a nesting pattern with a tubular structure,which was an adenosquamous carcinoma structure.One patient survived 4 months after surgery and the other patient died of intrahepatic tumour recurrence 7 months after surgery.Conclusions The diagnosis of primary hepatic ASC relied on histopathological examination.Surgical resection was safe and feasible.
10.Efficacy of precise sequential therapy for primary liver cancer
Hongyi ZHANG ; Zhiqiang FENG ; Hongyi ZHANG ; Hui ZHANG ; Mei XIAO ; Yuying ZHEN ; Xinbao XU ; Xiaojun HE
Chinese Journal of Digestive Surgery 2012;11(1):73-78
Objective To investigate the construction and implementation of the concept of precise sequential therapy for primary liver cancer.Methods The clinical data of 207 patients with resectable liver cancer who were admitted to the Air Force General Hospital from May 2005 to June 2010 were retrospectively analyzed.Of all the patients,81 received conventional therapy (conventional therapy group),and the other 126 patients received precise sequential therapy (precise sequential therapy group).The conditions of the patients in perioperative phase,during postoperative sequential treatment and the period of follow-up between the 2 groups were compared.All data were analyzed by using the covariance analysis,analysis of variance,chi-square test or rank sum test.Results The detection rates of lesions with a diameter less than 1 cm,operation time,liver resection volume,intraoperative blood loss,rate of perioperative blood transfusion,duration of postoperative hospital stay,incidences of postoperative complications were 81% (22/27),( 186 ± 36) minutes,(75 ± 29) ml,( 189 ± 60) ml,24%(30/126),(21 ± 12)days and 13% (17/126) in the precise sequential therapy group,and 18% (2/11),(222 ± 30)minutes,(133 ±88)ml,(327 ±46)ml,51% (41/81),(26 ± 17)days and 20% (16/81) in the conventional therapy group,respectively,with significant differences between the 2 groups (F =10.876,7.390,46.996,31.025,14.556,6.315,4.017,P < 0.05).No significant difference was observed on the levels of alanine transaminase,albumin and the Child-Pugh score before and after the intervention in the precise sequential therapy group,but significant differences were observed in the conventional therapy group.The 1-,2-,3-year tumor recurrence rates and the 1-,2-,3-year survival rates were 17% (21/126),22% (17/76),26% (8/31) and 87% (110/126),87% (66/76),84% (26/31) in the precise sequential therapy group,and 31% (25/81),38% (27/71),48%(31/65 ) and 77% (62/81),75% (53/71 ) and 60% (39/65) in the conventional therapy group,respectively.There were significant differences in the prognosis of the patients in the 2 groups ( x2 =4.958,4.292,4.168,4.062,3.640,5.470,P < 0.05 ).Conclusion Through accurate assessment of the patients' condition before surgery,precise hepatectomy and precise postoperative intervention,the goal of effective control of tumor recurrence,maximum protection of the liver function and improvement of the survival rate can be achieved.