Humans ; Iron Overload ; diagnosis ; etiology ; therapy ; Myelodysplastic Syndromes ; diagnosis ; metabolism ; therapy

Objective To detect the expression of miR-18a and estrogen receptor alpha (ER alpha) in single and multiple uterine flesh tumor tissues, discuss the relationship between miR-18a and ER alpha, and their effect in single and multiple uterine fibroids.Methods The expression of miR-18a and ER alpha in single and multiple uterine fibroids tissue paraffin section were detected by in situ hybridization method and immunohistochemical method, respectively.And the correlation between the miR-18a and ER alpha were evaluated.Results The expression of ER alpha in multiple uterine fibroids group was significantly higher than that of single uterine fibroids tissues (P<0.05);while miR-18a was weaker than that of single uterine fibroids tissues(P <0.05).The correlation results showed that miR-18a expression was correlated negatively with ER alpha expression either in single(r =-0.4421) and multiple uterine fibroids(r =-0.4181).Conclusion The expression of miR-18a is low in multiple uterine fibroids, while ER alpha had high expression.miR-18a could bea new target for the treatment of multiple uterine fibroids.

China ; Humans ; Myelodysplastic Syndromes ; diagnosis ; therapy

Attention ; Humans ; Myelodysplastic Syndromes ; diagnosis ; Myeloproliferative Disorders ; diagnosis ; Neoplasms ; Prognosis ; World Health Organization

AIM: To explore the treatment method and timing for neonatal dacryocystitis in different month-old children.
●METHODS: A total of 180 children ( 207 eyes ) with dacryocystitis who were admitted to our hospital between Jan. 2010 and Dec. 2014 were selected as the study subjects. According to months of age, they were divided into <3 months old group (n=41, 45 eyes), 3-6 months old group (n=75, 91 eyes) and 6-12 months old group (n=64, 71 eyes). Three groups were respectively treated with lacrimal massage combined with antibiotics, lacrimal duct washing with pressure and lacrimal duct probing, etc. The curative effect was compared between different months old children with dacryocystitis. The cure rate was statistically analyzed. The mean times of treatment was recorded and the best time for treatment was summarized.
●RESULTS:1) Hasner valvular obstruction was common in different months old children, and the proportion was higher than that of children with nasolacrimal duct bony part obstruction (P<0. 05). The proportion of children with nasolacrimal duct bony part obstruction in 6 - 12 months old group was higher than that in <3 months old group and that in 3-6 months old group (P < 0. 05). The proportion of children with Hasner valvular obstruction was lower than that in<3 months old group and that in 3-6 months old group (P<0. 05); 2) the number of cured eyes in < 3 months old group was higher than that in 3-6 months old group and that in 6-12 months old group ( P<0. 05), and the number of cures eyes in 3-6 months old group was higher than that in 6-12 months old group ( P<0. 05);3) the effective rates of lacrimal duct washing with pressure in <3 months old group and 6-12 months old group were similar, lower than that in 3-6 months old group (P<0. 05); 4) the effective rates of lacrimal duct probing in the three group were higher than 90% ( P>0. 05);5) the times of treatment in 3-6 months old group and 6-12 months old group was more than that in <3 months old group (P<0. 05), and that in 6-12 months old group was more than that in 3-6 months old group ( P <0. 05).
● CONCLUSION: For < 3 months old children with dacryocystitis, conservative treatment like lacrimal massage should be preferred. For children without effect, lacrimal duct washing with pressure and lacrimal duct probing should be given; For 3-6 months old children, lacrimal duct washing with pressure is preferred, and for 6 - 12 months old children, lacrimal duct probing is recommended.

Humans ; Myelodysplastic Syndromes ; Societies, Medical ; United States

Objective: To investigate the malignant phenotype of human glioma cells by microRNA-7 (miR-7) silencing epidermal growth factor receptor (EGFR)/ phosphatidylinositol kinase-3 (PI3K) pathway. Methods: The human U251 glioma cells were transfected with pri-miR-7. The expression of miR-7 was analyzed by real-time fluorogenic quantitative-PCR (RFQ-PCR). The expressions of epidermal growth factor receptor (EGFR), PI3K and AKT2 proteins were detected by immunocytochemistry and Western blotting. The cell growth curves were drawn, and the cell cycle distribution induced by miR-7 was determined by flow cytometry (FCM). The cell migration ability and tumorigenicity were detected by Transwell chamber assay and soft agar colony assay, respectively. Results: The result of RFQ-PCR showed that the expression level of miR-7 was up-regulated in human glioma cells transfected with pri-miR-7. The expression levels of EGFR, PI3K and AKT2 proteins were down-regulated in glioma U251 cells transfected with pri-miR-7 (P < 0.05). The cell proliferation rate was slowed down, and the proportion of S phase cells was reduced. The abilities of cell migration and soft agar colony formation of U251 cells transfected with pri-miR-7 were significantly reduced (P < 0.05). Conclusion: MiR-7 transfection can effectively silence the expressions of key members of EGFR/PI3K pathway and reverse the malignant phenotype of U251 cells and which is expected to become a new choice of glioma gene therapy. Copyright© 2011 by TUMOR.

Objective To explore the risk factors of acute organophosphorus pesticide poisoning(AOPP). Method The patients with acute organophosphorus pesticide poisoning,admitted from September 2004 to August 2005,were retrospectively analyzed.The 87 patients were divided into groups according to the presence or absence of combined conditions including hypotension,hypoxemia or metabolic acidosis.Acute physiology and chronic health evaluationⅡ(APACHEⅡ)score was calculated.The chi-square test was used to examine the mortality between those groups.Results The total in-hospital mortality of 87 acute organophosphorus pesticide poisoning patients was 21.8%.The mean APACHEⅡscore was(7.58?5.32)in the 68 survivors and(21.17～9.46)in the 19 dead,there were significant differences between the survivors and the dead(t=9.25,P20 was 65.2%(15/23),andit was 6.3% (4/64)in patients with APACHEII score

Objective To evaluate the cranioplasty with Titanium mesh.Methods Clinical studies of 110 cases who accepted cranioplasty with Titanium mesh (2000-2001) were reviewed retrospectively.Results A satisfactory moulding with no complication occurred in 91%(100/110). The most common recent complication of cranioplasty with Titanium was subcutaneous hematoma(19/110); the next was epidural hematoma(3/110), intracerebral hematoma(3/110) and brain contusion(1/110). 69 cases more than 1 year after cranioplasty were followed, no one appeared long term complications such as infection, exposure or mobilization of the mending material.Conclusion The results of cranioplasty with Titanium mesh are satisfactory, the recent complications of the operation are mainly related to incompletely hemostasis, the long term complication occurred rarely.

Adult ; Carcinoma, Neuroendocrine ; Carcinoma, Small Cell ; Ear Neoplasms ; Ear, Middle ; pathology ; Female ; Humans