1.Design and appliation of a kind of bone age automatic analysis and assessment device.
Wei LUO ; Mengxiong LI ; Peng YUN ; Hu XIAO
Chinese Journal of Medical Instrumentation 2014;38(4):264-266
OBJECTIVEThe device of a bone age film of analysis and process was designed, can calculate the expected patient's height and identify growth level and development.
METHODSSelect the DR bone age of 100 children of 6-13 years old, used semi Lagrange algorithm of target region of interest on bone age piece (ROI) for image analysis, compared with 2 pediatric endocrinologists (A, B) who used TW3 artificial to judge bone age (two methods were detected 2 times), and report the results.
RESULTSBone age assessment process, forecast error of bone age reduced to 0.12 years.
CONCLUSIONSThis device can quickly calculate the expected patient's height and identify his growth level, improve the speed and accuracy of bone age assessment, especially in the poor medical conditions in rural and remote areas.
Adolescent ; Age Determination by Skeleton ; instrumentation ; Child ; Equipment Design ; Humans
2.Curative Effect of Anti-Helicobacter Pylori Treatment on Recurrent Abdominal Pain and Chronic Superficial Gastritis in Children
zhi-hong, HU ; ming, SHEN ; yun, MIAO ; yong-mei, XIAO
Journal of Applied Clinical Pediatrics 2004;0(07):-
0.05).There was significant difference of curative effect before and after treatment of children with RAP(?2=6.74,P
3.Epidemiological Survey of Nodular Gastritis and Duodenal Ulcer before and after Eradicative Treatment of Hpylori Infection
yong-mei, XIAO ; ming, SHEN ; yun, MIAO ; zhi-hong, HU
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To investigate the prevalence of nodular gastritis,duodenal ulcer before and after eradicative treatment of helicobactor pylori(Hp) infection in children with gastroscopy.Methods This was a retrospective analysis of 1 275 children,age ranging 1 to 16 years old,collected from our hospital in recent 10 years,detecting rates of before eradicative treatment and after eradicative treatment were analyzed.Gastroscopes were analyzed with regard to a possible association with the infection.Results The detecting rate(11.89%) of duodenal ulcer after eradicative treatment was lower than that(17%) before eradicative treatment(P
5.Impact of iodine deficiency on expression of thyroid stimulating hormone β splice variant in BALB/c mice
Xiao-hua, ZHUO ; Xin, LIU ; Zhi-mei, HU ; Xiao-yi, ZANG ; Yun, SUN ; Lan-ying, LI
Chinese Journal of Endemiology 2012;31(3):251-254
ObjectiveTo find out if the immune system derived thyroid stimulating hormone(TSH) β splice variant(TSHβ-Ⅴ) would be regulated by circulating thyroid hormone levels to get a further understanding of the function and mechanism of this TSHβ-Ⅴ in thyroid homeostasis.MethodsA total of 20 weaning Balb/c mice (half male and half female) were selected and randomly divided into two groups according to their body mass and gender(n =10).Mice of control group were fed with common diet and deionized water.Mice of the low-iodine(LI) group were fed with low-iodine diet(containing iodine 20 - 40 μg/kg,iodine-intake about 0.25 μg/d) and deionized water.The experimental period was 3 months.At the end of the experiment,mice were executed and the blood was collected to observe the levels of TSH and thyroid hormone by chemiluminescence immunoassay (CIA) ; bone marrow (BM),peripheral blood(PBL),thyroid gland and pituitary were collected to assay the TSHβ-Ⅴ mRNA expression by real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR).ResultsThe serum free thyroxine(FT4) and total thyroxine(TT4) levels in LI group of mice[(0.47 ± 0.70)nmol/L,(2.41 ± 0.28)pmol/L] were significantly lower than that of the control group of mice [(55.2 ± 3.68) nmol/L, (32.72 ± 1.02) pmol/L,t =43.81,86.04 、all P < 0.01 ] and the serum total triiodothyronine(TT3) and free triiodothyronine(FT3) reduction in LI group of mice[ (0.76 ± 0.08)nmol/L,(4.01 ± 0.40)pmol/L] were significantly lower than that of the control group of mice [ (1.10 ± 0.06)nmol/L,(5.40 ± 0.38)pmol/L,t =9.81,7.5 1,P < 0.01 ].Iodine insufficiency strongly elevated the serum TSH in LI group of mice[ (35.67 ± 17.39)mU/L] than that in control group of mice[ (0.24 ± 0.10)mU/L,t =- 6.11,P < 0.01 ].The mRNA levels of TSH β-Ⅴ in BM (9.62 ± 0.60) and in PBL( 9.25 ± 0.83 ) of LI group of mice were lower than those in control group of mice (7.69 ± 0.36,7.11 ± 0.41,t =6.77,5.64,P < 0.01),while the mRNA level of TSH β-Ⅴ in pituitary of LI group of mice (1.99 ± 0.61) was increased compared with that in control group of mice (5.75 ± 0.98,t =- 8.02,P< 0.01).Compared with control group of mice(9.12 ± 0.62),the level of thyroid TSH β-Ⅴ mRNA in LI group of mice (9.32 ± 0.91 ) was not significantly changed (t =0.45,P > 0.05).There was no detectable native TSHβ in BM,PBL and thyroid.The mRNA level of native TSHβ in pituitary in LI group of mice( - 7.17 ± 1.78) was dramatically elevated compared to that in control group of mice( - 1.43 ± 0.51,t =- 7.60,P < 0.01 ).ConclusionsThe mRNA levels of TSHβ-Ⅴ are suppressed in BM and PBL in low iodinediet induced hypothyroidism mice,which suggest that immune system derived TSHβ-Ⅴ may be more important thannative TSHβ in immune-thyroid regulation.
6.Effects of GM6001 on haze formation after excimer laser epithelial keratomileusis in rabbit
Xiao-ling, CHEN ; Xiao-wei, GAO ; Yu-kun, HU ; Yun-lin, GUO ; Xiao-hong, LI ; Wen-jing, LI
Chinese Journal of Experimental Ophthalmology 2011;29(7):631-634
Background More and more attentions are paid to the effect of matrix metalloproteinases (MMPs) in the process of wound healing after excimer laser.Objective The present study was to investigate the effects of GM6001,a matrix metalloproteinases inhibitor,on haze formation after excimer laser epithelial keratomileusis (LASIK).Methods LASEK of -10.00 diopter was bilaterally performed on 27 New Zealand white rabbits and then divided randomly into the GM6001 group,Fluorometholone (FML) group and normal control group.Corneal haze was graded under dim light at 2 weeks,4 weeks and 8 weeks after operation,and the number of eyes in different grades of haze were compared among the three groups.Six corneal samples were harvested in each group for confocal microscope examination at 2,4,8 weeks.The corneal histopathological examination was carried out with the optical microscope,and the ultrastructure of the cornea was examined under the transmission electron microscope.Results The numbers of eyes of different corneal grades of haze at 2,4 or 8 weeks after surgery were significantly different among the three groups (P<0.05).Compared with the normal control group,the corneal haze grades in the GM6001 group and FML group were apparently lower (P<0.01),but there was no significant difference in the numbers of eyes of different corneal grades of haze between the GM6001 group and FML group (P>0.01).The numbers of keratocytes in the GM6001 group were apparently lower than those in the normal control group and FML group in 2,4 or 8 weeks after operation (P<0.05).No significant difference in the keratocytes was seen between the normal control group and FML group at various time points (P>0.05).Corneal epithelial cell morphology,disordered arrangement of collagen fibers in GM6001 group and in FML group were less pronounced than in the negative control group.Conclusion GM6001 has an inhibitory effect on the formation of corneal haze after LASEK which suppresses proliferation and production of keratocytye with the similar efficacy as FML.
8.Significance of COX2 and beta-catenin expression in nephroblastoma.
Xiao-Li HU ; Lin-Sheng ZHAO ; Lan-Yun SONG ; Pei-Ru NING
Chinese Journal of Pathology 2008;37(10):698-699
Child
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Child, Preschool
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Female
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Gene Expression Regulation, Neoplastic
;
Humans
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Infant
;
Male
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Wilms Tumor
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genetics
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metabolism
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Wnt Proteins
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genetics
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metabolism
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beta Catenin
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genetics
;
metabolism
9.Epigastric heteropagus twins: report of a case.
Xiao-li HU ; Lan-yun SONG ; Lin-sheng ZHAO ; Pei-ru NING
Chinese Journal of Pathology 2009;38(7):491-492
Abnormalities, Multiple
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pathology
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surgery
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Humans
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Infant, Newborn
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Male
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Twins, Conjoined
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pathology
;
surgery
10.Optimization of spraying dry technology of Biqiu ranules ethanol extract by Box-Behnken response surface method.
Yan-jun ZHANG ; Li-li LIU ; Jun-hua HU ; Yun WU ; En-xiang CHAO ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(18):3585-3589
With inlet temperature, specific gravity, feeding speed as independent variables, the comprehensive evaluating indexes of content of schisandrin and arctiin as dependent variable, the experimental data were fitted to a second order polynomial equation. Based on establishing the mathematical relationship between the comprehensive evaluating indexes and respective variables, Box-Benhnken central composite test and response surface analysis method was employed to optimize the spray drying technology of Biqiu granules ethanol extract. The optimal drying parameter was as follows: the inlet temperature was 175 degrees C, the specific gravity was 1.10, feeding speed was 32 r x min(-1). Under these conditions, the comprehensive evaluating indexes of spraying dry processes was 92.68, which was close to the model prediction. The spraying dry technology of Biqiu granules ethanol extract optimized by response surface methodology was accurate and feasible, which provided theoretical experiment basis for the industrialization production.
Chemistry, Pharmaceutical
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methods
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Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Ethanol