OBJECTIVE: To establish an HPLC-ELSD method to determine a new compound named Fructopyrano-(1 → 4)-glucopyranose in Radix Isatidis. METHODS: The HPLC method was conducted on an Agilent XDB-C18 column (4.6 mm × 150 mm, 5 μm), with methanol-water (5:95) as the mobile phase. The drift tube temperature was 115°C and flow fate of carrier gas was 3 L · min-1. RESULTS: The calibration curve was linear for FG in the range of 8.40 - 21.00 ng (r = 0.9990). The recovery was 97.27% - 102.72%. CONCLUSION: This method is accurate, reliable, rapid, sensitive, reproducible, and can be used for the determination of FG in Radix Isatidis. Copyright 2012 by the Chinese Pharmaceutical Association.

Background Diabetic retinopathy (DR) is a common complication of diabetic mellitus,but its pathogenesis is still unclear.Adiponectin may restrain inflammatory reaction and reduce adhesion of vascular endothelial cell to influence diabetic microangiopathy.Relation between adiponectin and nitric oxide synthase (NOS)is less reported.Objective This study was to observe the effect of adiponectin on the expression of NOS in the retinas of diabetic rats.Methods Forty 8-10 weeks Sprague-Dawley (SD) female rats were collected.The rats were randomized into the normal control group (10 rats),adiponectin group (15 rats) and diabetic model control group (15 rats) using the random number table method.Tetraoxypyrimidine was intraperitoneally injected to establish the diabetic model in the rats of the adiponectin group and diabetic model control group,and 10 μg/kg of adiponectin was then injected into the rats of the adiponectin group.Western blot was used to detect the expression of the adiponectin protein in the rat retinas,and the expression of NOS in rat retina was located by immunochemistry.The use of the animals complied with the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission.Results The relative amount of adiponectin (adiponectin/β-actin)in the retinas was 0.85 ± 0.21,0.79 ± 0.17 and 0.42 ± 0.08,respectively,in the normal control group,adiponectin group and diabetic model control group,with significant differences among the 3 groups (F =4.236,P =0.000).The adiponectin/β-actin ratio in the retinas of the diabetic model control group was significantly declined in comparison with the normal control group and adiponectin group (q =6.615,P =0.000 ; q =6.026,P =0.000).The NOS levels (A value) in the retinas were 0.244 ± 0.035,0.262 ± 0.032 and 0.367 ± 0.066,respectively,in the normal control group,adiponectin group and diabetic model control group,showing a significant difference among them (F =3.752,P =0.001).The A value of NOS in the diabetic model control group was significantly increased in comparison with the normal control group and adiponectin group (q =3.488,P =0.002 ; q =3.079,P =0.005).NOS expression was localized to the inner nuclear layer and retinal ganglion cell layer.Conclusions Adiponectin reduces NO content in diabetic rat retinas by regulating NOS expression.

Objective To explore the physical growth in infants with eczema who treated with regular use of glucocorticoid.Methods A total of 115 full-term infants newly diagnosed with eczema were included in this study and treated with topical glucocorticoids regularly.The severity of eczema was assessed and anthropometric indices were measured regularly.Results Both weight for age (WAZ) and length for age (LAZ) at birth and 6-month-old were higher than those of World Health Organization (WHO) child growth standards.However,the LAZ at diagnosis and 3 months after treatment were lower than that of WHO child growth standards.The weight,length,and the growth rate from birth to the time of diagnosis were all lower than those of WHO child growth standards.While the growth rate after treatment for 3 to 6 months was higher than that of WHO child growth standards.The increases of WAZ and LAZ were significantly different among three periods:at birth to diagnose,at diagnosis to 3 months after treatment,and 3 months to 6 months after treatment (P＜0.001).Conclusion In the early stage of eczema,the growth rate was decreased when the symptoms were not controlled.However,after treatment,the growth rate was increased.It is suggested that treatment of regular topical glucocorticoids has no adverse effects on growth of eczema infants,and also is beneficial to the catch-up growth of infants.

Complexity algorithm and wavelet package transform are discussed in this paper. To improve the classification ability of complexity algorithm in motion pattern recognition, it's used together with wavelet package transform. This method shows a better performance than a single complexity algorithm.

Objective Study on methylenetetrahydrofolate reductase(MTHFR)gene polymorphism in coronary heart disease.Methods Detect the MTHFR C677T gene polymorphism by PCR-RFLP in 73 cases of healthy individuals and 87 cases of coronary heart diseas(CHD).Results At the site of 677,the T allele frequencies are 18.5%,36.1%,respectively,for healthy individuals and 87 cases of coronary heart disease.The frequecies of MTHFR CT genotype and T allel were significantly higher in disease groups.There are significant differences between disease group and control group(P

Bile acids (BAs) are a major component of bile salt, which plays a vital role in the metabolism of lipids in humans. Ninety-five percent of bile acids are recycled by the enterohepatic circulation (EHC), and therefore EHC is essential for bile acid homeostasis. There are four transporters that mediate the transmembrane transport of bile acids, each of which plays an important role in the enterohepatic circulation. Gene defects in bile acid transporters can lead to disorders of the enterohepatic circulation, ultimately leading to clinical phenotypes such as metabolic diseases and even death. Bile transporter expression is altered in patients with various metabolic disease states, suggesting that disruption of bile acid transporters may be a pivotal pathological mechanism for the development of metabolism diseases. Thus, many drugs targeting bile acid transporters are being developed. We provide a concise overview of the progress of bile acid transporters research, discuss the relationship between different bile acid transporters and disease development, and summarize the current progress in drug development targeting bile acid transporters.

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PurposeTo evaluate the motion correction algorithm (snapshot freeze, SSF) and multi-cycle reconstruction on the imaging quality and interpretation of coronary CT angiography (CCTA).Materials and Methods Sixty patients with suspected coronary heart disease were divided into two groups according to the heart rate. Twenty-ifve patients with heart rate ≤65 beats per minute underwent prospectively ECG-gated CCTA on gemstone CT. Image quality and interpretability of standard (STD) and SSF reconstructions at 75% R-R interval were compared. Thirty-five patients with heart rate>65 beats per minute underwent retrospective ECG-gated CCTA. Image quality and interpretability of STD and SSF reconstructions at 45% and 75% R-R interval were compared. The image qualities and interpretability were assessed based on coronary artery segment, coronary artery bifurcation and overall coronary artery.Results For 25 patients with prospectively ECG gated CCTA, SSF reconstructions showed higher interpretability than STD reconstructions on per-segment level [96.3% (341/354)vs 90.7% (321/354),P<0.01]. Image qualities with SSF were higher than that with STD reconstructions for left anterior descending (LAD), left circumflex (LCX) and right coronary artery (RCA). There was no statistic significance for left main coronary artery (P>0.05). For 35 patients with retrospective ECG-gate CCTA, SSF reconstructions at 45% R-R interval showed higher interpretability (P<0.01,P<0.001). But there were no signiifcant difference for 75% R-R interval images (P>0.05). Image qualities with SSF were higher than that with single and double sector STD reconstructions (P<0.001). The image qualities were signiifcant different among SSF, single and double sector STD reconstructions for 45% R-R interval images of LM (P<0.01), but it was similar for 75% R-R interval images (P>0.05). There were signiifcant different for LAD, LCX and RCA on both 45% and 75% R-R interval images (P<0.01,P<0.001). Conclusion SSF may improve the image quality and interpretability of CCTA images using prospectively and retrospective ECG-gated techniques, especially for improving the image quality.

Objective To evaluate beam-hardening (BH) artifact reduction of myocardium in coronary computed tomography angiography(CCTA)with single-source dual-energy CT. Methods Thirty patients received CCTA on single-source dual-energy CT with findings of coronary artery stenosis<50%were enrolled in this study prospectively. Scanning mode was gemstone spectral imaging (GSI), single-source instantaneous(0.5 ms)kVp(140 kVp and 80 kVp)switch. The original images acquired by scanning were
reconstructed into monochromatic energy (60,70,80,90,100,110,120,130,140 keV) left vertical short-axis images via 40% ASIR and the polychromatic left vertical short-axis images were conventionally reconstructed. CT values were measured across multiple segments (basal anterior, basal lateral, basal inferior , basal septal, mid anterior, mid lateral, mid inferior , mid septal, apical anterior, apical lateral, apical inferior , apical septal and apex)of left ventricle wall at varying monochromatic energy levels and polychromatic images, and then the left ventricular myocardial average CT value and BH objective value were calculated retrospectively:BH1=CT value of mid inferior wall-CT value of basal inferior wall ,BH2=CT value of mid septal wall-CT value of mid inferior wall. BH subjective rating were evaluated by two radiologists independently. Single sample t test was used to compare the difference of myocardial CT values among 13 segments with the left ventricular myocardial average CT value on polychromatic images ;Kruskal-Wallis test was used to compare the difference of CT values among thirteen different segments of myocardium on fixed monochromatic energy images; Wilcoxon rank test was used to compare the difference of BH objective value and subjective rating between monochromatic images with polychromatic images. Results On polychromatic images, the mean myocardial CT value was(73 ± 25)HU, the CT value of basic inferior[(58±23)HU], basic septal[(85±21)HU], mid septal[(89±24)HU], apical anterior[(64±23)HU]and apex [(61 ± 24)HU ] were different from the mean myocardial CT value(t value were -3.76,2.89,3.50,-2.30, -2.86,P all<0.05),the differences of CT value between other myocardial segments and the mean myocardial CT value had no statistical significance(P all>0.05). The differences of CT value of different myocardial segments had statistical significance at 60 to 80 keV images(P all<0.05), the differences of CT value of different myocardial segments had no statistical significance at 90 to 140 keV images(P all>0.05), suggesting that the non-uniformity of CT value among different segments was improved. On polychromatic images,BH1 M(P25,P75)was 11(6,28),BH2 M(P25,P75)was 19(1,29) HU. BH1 was improved on 90 to 140 keV images while BH2 was improved on 70 to 140 keV images, the difference had statistical significance compared with the polychromatic images(P all<0.05). BH1,BH2 decreased with the increasing of monochromatic energy level on 60 to 100 keV images, then increased a little on 110,120 keV images, and hit bottom on 130 keV images with the value of 5.20,0.34 HU ,finally exist a slight increase on 140 keV images again. On polychromatic images,BH1,BH2 subjective rating M(P25,P75)both were 1(1,2), BH1 subjective rating was improved on 70 to 140 keV images while BH2 subjective rating was improved on 90 to 140 keV , the difference had statistical significance compared with the polychromatic images(P all<0.05). Conclusion Compared with the polychromatic images,monochromatic energy images of CCTA with dual-energy CT resulted in significant BH artifact reduction and improvements in the uniformity in the myocardium, and 130 keV is the optimal Monochromatic energy.