Objective:To investigate the effect of X-irradiation on Adhesion molecules and their related biological characteristics of Human Lung.Methods:A549 cells were irradiated with varying doses of X-rays.MTT assay was employed to evaluate the effects of X-irradiation on the proliferative inhibition of A549 cells.The immunocytochemical method was employed to analyze the protein expression levels of E-cadherin and ?-catenin.The transwell chambers were employed to evaluate the invasion of A549 cells.Results:X-irradiation could inhibit the proliferation of A549 cells in a dose-dependent manner.The protein expression of E-cadherin and ?-catenin in A549 were higher than that in control group(P

Objective:To explore the application effect of 3D printing teaching model on spinal surgery teaching.Methods:A total of 50 eight-year clinical medical students of Batch 2015 were selected and randomly divided into two groups. Traditional teaching mode was used in the control group and 3D printing teaching model was used in the experimental group. The performance of graduation from the department, teaching experience indicators and teaching satisfaction were compared between the two groups. SPSS 22.0 was used for t test. Results:The performance of the experimental group was higher than that of the control group ( P < 0.05). The scores of teaching experience indicators in the experimental group were all higher than those in the control group ( P<0.05). The satisfaction rate of the experimental group was higher than that of the control group ( P < 0.05). Conclusion:3D printing teaching model can significantly improve students' theoretical knowledge assessment and surgical design evaluation, and improve the teaching effect and students' satisfaction.

Cerebral white matter damage is the most common form of brain injury in premature infants,which is the main cause of neurological and mental injury and cerebral palsy.Imaging examination plays an important role in the diagnosis and follow-up of white matter damage.Because of the safe and accurate characteristics,nuclear magnetic resonance has become the most common imagine form.Different from the traditional nuclear magnetic resonance technique,diffusion tensor imaging (DTI) can get quantitative analysis of cerebral white matter fiber bundle in vivo.This technology has become a powerful tool for the evaluation of white matter damage.The article summarizes the application of DTI in the neurodevelopmental evaluation of cerebral white matter damage in premature infants.

OBJECTIVE: To optimize the formulation of ketamine hydrochloride for intranasal administration that was of effective anesthetic effect.METHODS: Using ketamine hydrochloride injection as basic preparation,various nasal absorption enhancer or viscosity modifiers(0.5%,1% and 2% azone,EDTA,carbomer,2-hydroxypropyl-?-cyclodextrin,carboxymethyl cellulose) were added to make different nasal drops.After intranasal administration,the anesthetic effect was evaluated to find the optimum formulation.The behavior of anesthetic induction period and anesthetic index were used to evaluate the anesthetic effect.RESULTS: The perfect anesthetic effect was achieved when the formulation was prepared by adding 1% azone in ketamine hydrochloride injection(at a dose of 10 mg?kg-1).The anesthetic time was long.The muscle relaxing time,eyelid reflecting disappearing time,pain disappearing time and righting reflecting disappearing time were(21.4?7.1) min,(11.4?4.6) min,(15.6?5.7) min and(12.0?4.8) min,respectively.CONCLUSIONS: Optimal formulation of ketamine hydrochloride for intranasal administration was obtained when 1% azone was added as an absorption enhancer.

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Acrylonitrile ; toxicity ; Animals ; Male ; Mice ; Mice, Inbred Strains ; Testis ; drug effects ; pathology

Objective To construct the eukaryotic expression vector of Notch1intracellular domain,p3XFLAG?CMV7?NICD1,so as to prepare for the further research and exploration of effect of Notch1 on promoting epithelial?mesenchymal transition of human lens epithelial cells. Methods The cDNA fragment was reversely transcribed by RT?PCR from total RNA extracted from the SRA01/04 cells and was encoded with the specific am?plification?targeted NICD1was obtained from the SRA01/04 cells,then the cDNA fragment was inserted into p3XFLAG?CMV7 to transcribe Esche?richia coli DH5α. And the recombinant plasmid was extracted after bacterial screening by LB plating medium and confirmed by the restriction endo?nuclease digestion and DNA sequencing. Results The target gene obtained had the same molecular size as predicted. It was indicated that recom?bined p3XFLAG?CMV7 plasmid contained correct recombinant human Notch1 sequences and p3XFLAG?CMV7?NICD1 was constructed success?fully. The western blotting showed protein NICD1 expressed in SRA01/04 cells transfected with p3XFLAG?CMV7?NICD1. Conclusion The suc?cessful construction of p3XFLAG?CMV7?NICD1 will provide a foundation for a further study studies in on the effect relationship of Notch signaling pathway and in posterior capsular opacification(PCO)after cataract extraction.

Objective To explore the clinical significance of two new indexes including approximate entropy of oxygen saturation (SpO2ApEn) and saturation impairment time index (SITi) in assessing the severity of hypoxemia in obstructive sleep apnea hypopnea syndrome (OSAHS) patients.In addition,to investigate the correlation betewwn new indexes and other parameters.Methods The six hour polysomnography (PSG) of 500 patients with OSAHS and snorers in our hospital was retrospectively analyzed.According to the level of apnea hypopnea index (AHI),subjects were divided into four subgroups,namely 113 healthy controls,121 mild OSAHS,118 moderate OSAHS,and 148 severe OSAHA patients.SpO2 ApEn and SITi values among the four groups were compared.the correlations between SpO2 ApEn,SITi and other parameters were analyzed,including AHI,oxygen desaturation index (ODI),the lowest oxygen saturation(LSpO2) and the duration of oxygen saturation lower than 90％ (T ＜ 90％).Next,taking AHI as the gold standard,the significance of SpO2ApEn and SITi in assessing the severity of hypoxemia in OSAHS patients was explored by ROC curve analysis.Results SpO2ApEn and SITi between two each groups were significantly different (P ＜ 0.001) and increased synchronously with AHI.Spearman correlation analysis showed that SpO2ApEn and SITi had a good correlation with AHI (correlation coefficient r =0.765,r =0.678,P ＜ 0.O1).SpO2ApEn and SITi also had a good correlation with ODI,LSpO2and T ＜ 90％.According to the ROC analysis,the Boundary values of SpO2ApEn for mild,moderate and severe OSAHS patients were 16.70,17.81,and 20.03,respectively,and the corresponding SITi values were 3.685,4.055,and 4.445.Conclusion In this study,SpO2ApEn and SITi increased synchronously with AHI,SpO2ApEn and SITi had good correlations with AHI,ODI,LSpO2and T ＜90％.SpO2ApEn and SITi have important clinical significance for assessment of hypoxia severity in OSAHS patients.

BACKGROUND:Wtih hyaluronic acid and col agen by cellautocrine as endogenous scaffolds, cellsheet technology can eliminate the disadvantages of exogenous scaffolds. Human adipose-derived stem cells combined with cellsheet technology used in the study of ful-thickness skin defects have become the current hot spot.
OBJECTIVE:To summarize the research progress in adipose-derived stem cellsheet.
METHODS:Articles related to adipose-derived stem cells, cellsheet technology and tissue-engineered skin were retrieved in VIP information and PubMed database from January 1993 to April 2014. The key words were“adipose-derived stem cell, wound healing, tissue-engineered skin, cellsheet”in English and Chinese, respectively. Total y 27 relevant articles were analyzed.
RESULTS AND CONCLUSION:Adipose-derived stem cells derived from discarded human adipose tissue are immmunocompatible and multi-potent, rendering them ideal for regenerative medicine applications. cellsheet technology seemingly provides several advantages over traditional tissue reconstruction with biodegradeable scaffolds. Adipose-derived stem cells combined with cellsheet technology can enhance tissue vascularization, promote wound healing, the secreted extracellular matrix provides the microenvironment for the celldifferentiation and growth, so it is expected to build a real three-dimensional tissue-engineered skin.

Objective To summarize the diagnosis and treatment experiences of focal nodular hyperplasia (FNH).Methods The clinical data of 48 patients with FNH who were admitted to the Xiangya Hospital from February 2010 to August 2014 were retrospectively analyzed.All the patients received complete serologic tests,abdomen B ultrasound,computed tomography (CT) and magnetic resonance imaging (MRI) examinations.The pathological examinations were done on the patients undergoing surgical treatment.The surgical treatment was done on the unconfirmed FNH patients according to location of space-occupying lesions.The conservative treatment was done on the confirmed FNH patients without clinical symptoms.All the patients were followed up via outpatient examination and telephone interview up to September 2014,and the follow-up included the history review,clinical symptoms and medical examinations.Results Forty-seven patients had normal liver function and 1 had high level of total bilirubin (TBil).The serum tumor markers including AFP,CEA and CA19-9 in all the patients were negative.Fifty-five foci were detected by imaging examination,41 foci were single mass and 7 foci were multifocality.Forty-eight patients were definitively unconfirmed with FNH by B ultrasound.The results of CT examinations in 38 patients showed as follows:low density was in 31 patients and isodensity in 7 patients,including striped and radiated low density showing in the center of tumor in 20 patients and 3 patients with the confirmed FNH.The results of MRI examinations in 10 patients showed as follows:3 patients were confirmed as with FNH; all the 10 patients showed rather homogeneous enhancement in arterial phase; 5 patients were accompanied with radiated foci in the center of tumor,and showed high signal on T2WI,no enhancement in early arterial phase and enhancement in delayed arterial phase.The central scar showed low signal on T1WI and high signal on T2WI.Two patients were confirmed as with FNH by percutaneous trans-hepatic histopathological examinations.Forty patients without the confirmed FNH underwent surgical treatment,including 31 undergoing open hepatectomy and 9 undergoing laparoscopic hepatectomy.No patients had cirrhosis of liver.During operation,47 foci were detected,16 of which located at the left lobe of liver and 31 of which located at the right lobe of liver.Local tumor resection of liver was carried out on 30 patients,right hemihepatectomy or extended right hepatectomy on 7 patients,left hemihepatectomy on 3 patients and combined treatment of cholecystectomy on 12 patients.The operation time,volume of blood loss and diameter of foci were (78-255) minutes,(80-720) mL and (4.6 ± 1.6) cm (range,1.5-11.5 cm).Forty patients were confirmed as with FNH by pathological examination,and 8 patients were cured by conservative treatment.All the 48 patients were followed up for 21 months (range,1-56 months) and survived well without recurrence and complications.Conclusions The clinical symptoms of FNH are atypical and unobvious.The preoperative enhanced CT and MRI examinations can help improve the diagnosis and differential diagnosis of FNH.The surgical treatment could be performed on the patients with significant clinical symptoms and without definitive diagnosis,and the patients with definitive diagnosis should be treated by conservative treatment with the good prognosis.