1.Effects of different duration of sevoflurane anesthesia in neonatal period on long-term cognitive func-tion and hippocampal synaptic plasticity in rats
Xuelian PAN ; Bo ZHAO ; Yun XIAO ; Jiabao HOU ; Zhongyuan XIA
Chinese Journal of Anesthesiology 2016;36(9):1085-1088
Objective To investigate the effects of different duration of sevoflurane anesthesia in the neonatal period on the long?term cognitive function and hippocampal synaptic plasticity in rats. Methods Twenty?four pathogen?free healthy Sprague?Dawley rats of both sexes, aged 7 days, weighing 12-16 g, were divided into 3 groups ( n=8 each) using a random number table: control group ( group C) , sevoflu?rane anesthesia for 2 h group ( group S1 ) , and sevoflurane anesthesia for 6 h group ( group S2 ) . Group S1 and group S2 inhaled 2% sevoflurane for 2 and 6 h, respectively. Morris water maze test was performed at 30 days after the end of anesthesia ( postnatal day 37) to assess the cognitive function. After the end of the test, the rats were sacrificed, and hippocampi were isolated for determination of the expression of brain?de?rived neurotrophic factor ( BDNF) , postsynaptic density?95 ( PSD?95) and synapsin 1 in hippocampal tis?sues by Western blot. Results Compared with group C, the escape latency on 4th and 5th days of the test in group S1 and on 2nd-5th days of the test in group S2 was significantly prolonged, and the frequency of crossing the original platform was significantly decreased, and the time of staying at the platform quadrant was significantly shortened in S1 and S2 groups, the expression of BDNF, PSD?95 and synapsin 1 in hipp?ocampal tissues was significantly down?regulated in group S2 (P<0?05), and no significant change was found in the expression of BDNF, PSD?95 and synapsin 1 in hippocampal tissues in group S1 ( P>0?05) . Compared with group S1 , no significant change was found in the escape latency and frequency of crossing
the original platform (P>0?05), the time of staying at the platform quadrant was significantly shortened, and the expression of BDNF, PSD?95 and synapsin 1 in hippocampal tissues was significantly down?regula?ted in group S2 ( P<0?05) . Conclusion Short?time and long?time sevoflurane anesthesia both can induce long?term cognitive dysfunction in the neonatal period, and the severity is aggravated with prolonged anes?thesia; the partial mechanism is related to inhibition of the synaptic plasticity of hippocampal neurons of rats.
2.The neuroprotective effects of grape seed proanthocyanidin on rat brain injury caused by chronic intermittent hypoxia
Ya-Ning Zhao ; Xia Guo ; Hong-Yang Wang ; Lin Li ; Pan-Pan Zhang ; Xiao-Qing Han ; Wen-Qian Liu
Neurology Asia 2014;19(4):399-403
Chronic intermitted hypoxia including sleep breathing disorder leads to brain injury. This study explores
the potential therapeutic effects of grape seed proanthocyanidin as a neuroprotective agent. A rat model
of chronic intermittent hypoxia was employed, and the animals were given low or high doses of grape
seed proanthocyanidin. The ultrastructure changes in the brain, the biochemical components, and the
animal behavior were examined. The results showed that with hypoxia exposure, neuronal mitochondria
exhibited injuries at ultrastructural level, with increased malondialdehyde (MDA) content and reduced
superoxide dismutase (SOD) activity. Terminal deoxynucleotidyl transferase dUTP nick end labeling
(TUNEL) staining revealed increased cell apoptosis in hippocampus. In Morris water maze the animals
showed decreased learning abilities, when compared to normal control. The administration of grape
seed proanthocyanidin treatment reversed all these observed changes, and improved the learning
behavior. We concluded that grape seed proanthocyanidin could alleviate the brain injury caused by
hypoxia from sleep breathing disorder.
3.Pharmacological studies on extracts from Piper boehmeriaefolium var. tonkinense.
China Journal of Chinese Materia Medica 2004;29(6):578-580
OBJECTIVETo study sedative, analgesic, antidepressant and anti-inflammatory effects of the extracts from Piper boehmeriaefolium var. tonkinense.
METHODEtOAc soluble constituents and EtOH soluble constituents were administered 50, 100 mg x kg(-1) to mice respectively. The sedative effects were determined by autonomic action test. The analgesic effects were observed by the twisting test induced by acetic acid. The antidepressant effects were investigated by the forced swimming test. The anti-inflammatory effects were studied by auricle swelling induced by xylene.
RESULTEtOAc extracts could significantly lower autonomic action times, reduce squirming induced by acetic acid accumulated immobility time in forced swimming and auricle swelling values by xylene. But EtOH extracts could only decrease autonomic action times and accumulated immobility time.
CONCLUSIONThe active ingredients with sedation, analgesia, antidepressant and anti-inflammation in P. boehmeriaefolium var. tonkinense exist mainly in the EtOAc extracts.
Acetates ; Analgesics ; pharmacology ; Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Antidepressive Agents ; pharmacology ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Hypnotics and Sedatives ; pharmacology ; Male ; Mice ; Piper ; chemistry ; Plants, Medicinal ; chemistry
4.Effects of Apelin on glucose toxicity and islet cells PDX-1 expression
Xuangeng HUANG ; Yingrong LI ; Xiaofei ZHENG ; Hailin PAN ; Hongye SU ; Ning XIA ; Changqing XIAO
Chongqing Medicine 2016;45(33):4633-4635
Objective To study the effects of Apelin on glucose toxicity and islet cells PDX-1 protein expression.Methods The islet β cell line NIT-1 cells were incubated in the medium containing different glucose concentrations(normal glucose concentration group 5.6 mmol/L,high glucose concentration group 16.7 mmol/L,extremely high glucose concentration group 33.3 mmol/L) and +/-Apelin-36 respectively for 3 d.Then the basic insulin secretion amount of islet cells and their secretion amount after glucose stimulation were detected.The intracellular insulin content and the PDX-1 protein and mRNA expression were detected.Results Compared with the normal glucose group,the basic insulin secretion,secretion after stimulation and intracellular insulin in the high glucose group and extremely high glucose group were significantly decreased and PDX-1 protein expression was declined(P< 0.05);compared with non-adding Apelin group,the basic insulin secretion,secretion after stimulation and intracellular insulin in the adding Apelin high glucose group and extremely high glucose group were significantly decreased and PDX-1 protein expression was decreased(P<0.05);the insulin level in islet cells of 6 groups was positively correlated with PDX-1 protein expression and had no correlation with PDX-1 mRNA expression.Conclusion Apelin may participate in the glucose toxic effect by decreasing PDX-1 protein expression,causes the decrease of insulin secretion,thus plays a role in the pathogenesis of diabetes.
5.Clinical Application of Integrated Visual and Auditory Continuous Performance Test on Children with Attention Deficit Hyperactivity Disorder at Different Ages
xue-xia, PAN ; bin, WAN ; hong-wei, MA ; xiao-mei, DAI
Journal of Applied Clinical Pediatrics 2004;0(12):-
0.05).Coincidence both of them in subtypes of ADHD diagnosed by 2 different ways were lower than 50% in the 6.0-6.9 and over 10.0 years old groups,but coincidence both of them were higher than 60% in 7.0-7.9,8.0-8.9,9.0-9.9 years old groups.What's more,there were significant differences though ?2 variance analysis in subtypes of ADHD by 2 different ways(Pa
6.Reevaluation on the mechanical properties of dentine microstructure
Xiao-Song LUO ; Yi-Xia ZHANG ; Yu-Hong CUI ; Jun PAN
Journal of Medical Biomechanics 2011;26(2):181-188
Objective:It is demostrated that the porous protein-mineral mechanics model could provide more accurate prediction for biomaterial properties of dentine compared with the other established models.This paper would use the model to reevaluate the mechanical properties and its interacting mechanism of human dentine.Method:By using a porous proteinmineral mechanics model,the effect from the interactions between tubules,pedtubular and intertubular matrix on dentine microstructure was discussed.Results:The dentinal micromechanical properties were dependent on the tubular direction,and the absolute values of the stresses derived from the hydraulic and gas tubular pressures increased parabolically with theincreasing diameter of the tubules.It was also found that the effective elastic constants of the dentine microstructure would vary with the aging and the distribution of mineral and collagen within peritubular and intertubular matrix of detine.Conclusions:The theoretical analyses provided in this paper demonstrated that the microstructural characteristics of tubules,peritubular and intertubular dentinal matdx could have different influences on the micromechanical properties of human dentine,which showed the validity of porous protein-mineral mechanics model,and the limitation of some models that neglected the interacting mechanism.
7.Reevaluation on the mechanical properties of dentine microstructure
Xiao-Song LUO ; Yi-Xia ZHANG ; Yu-Hong CUI ; Jun PAN
Journal of Medical Biomechanics 2011;26(2):181-188
Objective:It is demostrated that the porous protein-mineral mechanics model could provide more accurate prediction for biomaterial properties of dentine compared with the other established models.This paper would use the model to reevaluate the mechanical properties and its interacting mechanism of human dentine.Method:By using a porous proteinmineral mechanics model,the effect from the interactions between tubules,pedtubular and intertubular matrix on dentine microstructure was discussed.Results:The dentinal micromechanical properties were dependent on the tubular direction,and the absolute values of the stresses derived from the hydraulic and gas tubular pressures increased parabolically with theincreasing diameter of the tubules.It was also found that the effective elastic constants of the dentine microstructure would vary with the aging and the distribution of mineral and collagen within peritubular and intertubular matrix of detine.Conclusions:The theoretical analyses provided in this paper demonstrated that the microstructural characteristics of tubules,peritubular and intertubular dentinal matdx could have different influences on the micromechanical properties of human dentine,which showed the validity of porous protein-mineral mechanics model,and the limitation of some models that neglected the interacting mechanism.
9.Intelligence level and intelligence structure of children with primary nocturnal enuresis.
Xiao-Mei DAI ; Hong-Wei MA ; Xue-Xia PAN
Chinese Journal of Contemporary Pediatrics 2007;9(5):433-435
OBJECTIVESome research has shown that there may be memory/caution (M/C) defects in children with primary nocturnal enuresis (PNE). This study aimed to investigate whether the defects affect the intelligence level and the intelligence structure in PNE children.
METHODSIntelligence tests were performed by means of Wechsler Young Children Scales of Intelligence (C-WISC) in 40 children with PNE and 40 age-matched normal children.
RESULTSThe full intelligence quotient (FIQ), verbal IQ (VIQ) and performances IQ (PIQ) in the PNE group were in a normal range and did not different from the control group. There were significant differences in the scores for digit extent, decipher, knowledge and arithmetics between the PNE and the control groups (P < 0.05). M/C factor in the PNE group was statistically lower than in the control group (93.44 +/-11.27 vs 100.03 +/-11.79; P < 0.05).
CONCLUSIONSThe total intelligence level of children with PNE was normal, but the M/C factor in the intelligence structure had some defects, suggesting that PNE may be related to the abnormity of executive function in the frontal lobe.
Adolescent ; Attention Deficit Disorder with Hyperactivity ; psychology ; Child ; Female ; Humans ; Intelligence ; Male ; Nocturnal Enuresis ; psychology
10.Goodpasture's syndrome: report of a case.
Yu-zhen DAI ; Mei-li CHEN ; Zhao-xia YU ; Xiao-an HU ; Run ZHANG ; Jing LU ; Tao-qiang PAN
Chinese Journal of Pathology 2009;38(4):278-279