Objective:①To study the expression of Intercellular adhesion molecule-1(ICAM-1)、Tumor necrosis factor-?(TNF-?)in the hypertrophic myocardium of rat from the level of transcription and translation and the expression of ICAM-1、TNF-? in the normal myocardium of rat,and compare them,find the law of expression,inquire into relation between myocardial hypertrophy and ICAM-1、TNF-?,and to explain myocardial hypertrophy's pathogenesis preliminarily.②To observe above items after using ACEI and inquire into pathogenesis of ACEI in reversing myocardial hypertrophy.Methods:30 SD rats were divided into 3 groups and one group contained 10 rats.The first group was myocardial hypertrophic group.The second was myocardial hypertrophic group that was treated with ACEI.The thirth was control.After 8 weeks,they were killed and cardiae specimen were obtained.The proteins of ICAM-1、TNF-? were detected by immunohistochemistry and(LV's) specimen were detected in ICAM1(mRNA、)(TNF-?)(mRNA) with RT-PCR.Results: The expression of proteins of ICAM-1、(TNF-?) in the first group increased and dropped in the second group.That of the third is the lowest.The expression of ICAM-1 (mRNA、)(TNF-?) mRNA was the same to above. Conclusion:ICAM1、TNF-? plays a role in myocardial hypertrophy pathogenesis.ACEI which lowers the expression of ICAM-1、TNF-? can results in reversing myocardial hypertrophy.

Objective To explore the antagonistic effect of Schistosoma japonicum soluble adult worm antigen (SWA)and solu-ble egg antigen (SEA)in the mice with type 1 diabetes.Methods The 24 successful modeling type 1 diabetes mice were randomly divided into three groups (A,B,C group,n=8).SWA and SEA of Schistosoma japonicum were prepared.Mice in A group were immunized by abdominal subcutaneous multi-point injection SWA.Mice in B group were immunized by abdominal subcutaneous multi-point injection SEA.And mice models of C group were immunized by PBS instead of antigen through abdominal subcutaneous injection.The mice got immunization once a week,a total of four times.4 weeks later,the mice were sacrificed,and serum speci-mens were collected for the determination of serum levels of IL-4 and IFN-γby double-antibody sandwich ELISA,while pancreas tissues were collected and the pathological changes were observed.Results The serum IL-4 level of B group [(23.87 ±4.85)pg/mL]was higher than C group [(4.39 ± 0.56 )pg/mL],with significant differences (P < 0.01 ),while the serum IFN-γ level [(271.85±26.04)pg/mL]was lower than C group [(362.79 ±32.50)pg/mL],also with significant differences (P <0.01).The serum IL-4 and IFN-γof A group were (5.09±0.37)pg/mL and (379.56±34.47)pg/mL,which had no difference with C group (P >0.05).The islet structure of mice in B group was not intact,however,the lymphocytic infiltration in B group was less than C group,and there was no lymphocytic infiltration in pancreatic islets in B group.Compared with C group,the pancreas of mice in A group did not have significant changes,lymphocytes infiltration was still visible in islets.The number of residual islet cells de-creased,and visible minority islet structure was destroyed.Conclusion SEA of Schistosoma japonicum has certain antagonism effect on type 1 diabetes in experimental mice.Its mechanism may be the reduction of Th1 response and the enhancement of Th2 response through increasing IL-4 level and decreasing IFN-γlevel.

Objective To improve the understanding of ileal conduit urinary diversion stomal varicose bleeding.Methods 3 male cases of ileal conduit urinary diversion stomal varicose bleeding were reported.The pathogenic mechanism,clinical features,diagnosis and treatment of this disease were reviewed and discussed with the relevant literature review.The ages were 59,45 and 68.All of them had ileal conduit urinary diversion because of muscle invasive bladder cancer.Ileal conduit urinary diversion stomal varicose bleeding were found at 6 years,6 months and 2 months after surgery.The bleeding was intermittent.All the stomal varicose were found in physical examination.The laboratory examinations showed abnormities of liver functions in all three cases.Image studies showed one with liver metastasis and two with liver cirrhosis.Portal venous hypertension was considered as the cause of bleeding.Results We controlled the bleeding by suturing the first patient's varicose.The patient was died 3 months later because of the advanced cancer.For the second patient,we controlled the bleeding by compressing the varicose.By the meantime,we reduced the portal venous hypertension with medication.The stomal varicose bleeding stopped when the liver function and the portal venous hypertension improved 2 months later.The third patient's stomal varicose were injected with sclerosants.There was no mnore stomal varicose bleeding within half-year follow-up.Conclusions Ileal conduit urinary diversion stomal varicose bleeding is usually found in the patients who have portal venous hypertension because of liver cirrhosis or liver metastasis.To control the bleeding,we can decrease the patient's portal venous hypertension.To control hemorrhage,we can use suturing,compressing,and sclerotherapy,which is an optional treatment.

Purpose:To investigate the expression of vascular endothelial growth factor (VEGF) and its relationship with proliferating cell nuclear antigen (PCNA) and the prognosis of colon cancer patients. Methods:The VEGF and PCNA as well as p53 expression were examined using immunohistochemistry with monoclonal antibody in 70 surgical samples of colon cancer. Results:VEGF expression was positive in 91.43% of the colon cancer patients. It had a positive relation with the p53 expression (rs=0.417, P

Human papillomavirus (HPV) is one of the most common infection of the virus in the reproductive tract,a growing number of studies found that HPV infection is closely related to female infertility.This paper mainly introduces the structure characteristics of HPV,the correlation of reproductive tract infection of HPV and infertility,the related factors of infertility by reproductive tract infection of HPV,the influence of early pregnancy and adverse pregnancy outcomes,the relationship with assisted reproductive technology,etc.

Objective To explore the effect of different concentrations of hypertonic saline for cerebral ede -ma on blood biochemical and osmotic pressure .Methods 60 cases were randomly divided into the 3% HS group, 7.5%HS group and the mannitol group .The patients were randomly assigned to infusion of 3% HS, 7.5% HS or 20%mannitol (all 2 mL/kg).Blood biochemical and osmotic pressure on patients were measured before and after the treatment among groups .Results Serum Na +, Cl -and osmotic pressure were significantly higher at 2 hours and 22 hours of reperfusion than at baseline (p<0.05) in the 7.5% HS group and the mannitol group .The difference were significant on serum Na +, Cl-and osmotic pressure at 2 hours and 22 hours of reperfusion in the 7.5% HS group and the mannitol group than in the 3% HS group(p<0.05).Conclusion 3% hypertonic saline had less effects on blood biochemical and osmotic pressure than other groups for patients of cerebral edema .It is worthy of popularization and application .

Acquired factor XIII deficiency disease is rare.One case with gum bleeding as the first symptom caused by acquired factor XIII de-ficiency disease was encountered.The case was analyzed and relevant literatures were reviewed.

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Objective: To elucidate the major reasons for unexpected death nude mice when they were used for establishing human metastatic cancer models. Methods: The fresh specimens of human renal cell carcinoma (RCC), prostate cancer (PC), and DU-145 cells were transplanted/injected into nude mice ecotopically and orthotopically. Tumorigenesis and pathological changes (including the symptoms, pathological sections, survival time, etc.) of mouse liver were investigated subsequently. Results: The tumorigenesis and metastasis rates were respectively 21.7% (35/161) and 1.2% (2/161) after implantation of RCC sample, and were respectively 100% (20/20) and 25% (5/20) after implantation of DU-145 cell line, while there was no tumorigenesis or metastasis after implantation of PC specimens. Liver pathological changes were found in 58.4% (94/161) of mice implanted with RCC samples and in 43.4% (46/106) of mice implanted with PC samples. No pathological lesion was found in mice implanted with DU-145 cells. The death peak of mice with pathological changes after implanting RCC and PC samples was consistent with that of the total mice used in this study, all occurring in the winter and spring of the year. Conclusion: The pathological changes of liver appear to be the major reason of unexpected death of the nude mice when they were used for establishing human metastatic cancer models. A specified pathogen-free environment is very important for establishment of the models.

Objective: To establish orthotopic mouse models of human renal cell carcinoma and to separate metastatic or non-metastatic renal cell carcinoma (RCC) from the same source. Methods: Surgical specimens/cell suspensions were transplanted into the various tissues of BALB/c nude mice (subcutis, cellular orthotopic injection into renal capsule, perinephrium, surgical orthotopic implantation into renal capsule). Tumorigenicity and metastasis were subsequently evaluated. Immunohistochemical analysis was employed to determine the expression of VEGF, bFGF, P16, Bcl-2 and C-met in the metastatic and non-metastatic RCC xenograft. Results: The incidences of tumorigenicity and metastasis of orthotopic model were the highest, being at 73.3% (11/15) and 20% (3/15), respectively. Compared with the non-metastastic RCC, VEGF expression was upregulated in the metastatic RCC (P<0.05), while the expression of C-met was downregulated significantly (P<0.05); the expression of bFGF, Bcl-2 and P16 was also downregulated but without significance. Conclusion: Mouse orthotopic transplantation is the most effective way for the growth and natural metastasis of human RCC. Primary and metastatic RCC from the same patient has been successfully harvested. Expression of VEGF in the metastatic tumor tissues is significantly higher than that in the non-metastatic RCC.