1.Role of ureaplasma urealyticum infection and maternal-fetal vertical transmission pathway in diseases in neonates
Yanchun SHAN ; Zhaosuan LI ; Zenghuang XIAO
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To assess the relationship between ureaplasma urealyticum (UU) infection, vertical transmission pathway and diseases in neonates.METHODS: Specimens of amniotic fluid and cervical swabs from 30 women at delivery, and nasopharynx and urinary mouth secretions from 161 neonates were cultured for UU. RESULTS: The UU positive rate of 30 women at delivery and 8 neonates from puerperas with UU infection were 26.67% and 62.7%, respectively. The detective rate of UU in normal neonates (26 of 130, 20%) was significantly lower than that in sick neonates (14 of 31, 46%) ( P
2.Mid-term follow-ups of hydroxyapatite extensively-coated femoral prosthesis for elderly patients
Yun-Li ZHU ; Hai-Shan WU ; Xiao-Hua LI ;
Chinese Journal of Orthopaedic Trauma 2004;0(08):-
Objective To evaluate the effects of hydroxyapatite (HA) extensively-coated prosthesis on elderly patients undergoing hip arthroplasty.Methods From February 1997 to May 1999,96 patients Were treated with hydroxyapatite (HA) coated prosthesis and followed up for their hip arthroplasty.Their results were studied clinically and roentgengraphically to evaluate chiefly their Harris scores,complications and thigh pain.The survival of prosthesis was evaluated according to Kaplan-Meier methods.Results The 60 cases were followed up for eight years on average(range,sevon to nine years).Their mean Harris scores were improved from 42 preoperatively to 90 postoperatively.Fifty-eihgt cases felt no pain at their hip,but two did at their thigh.No radiolucent lines were observed.All the prostheses achieved bony stabilization and remolding.Conclusions HA coated prosthesis can achieve fine fixation,because it helps bone conduction,resists axial force and torsion,and reduces operative risks.
3.Studies on Mycoplasma genitalium infection in patients with chronic non-bacterial prostatitis
Shu-Xia ZHONG ; Shan-Shan LI ; Hua ZHENG ; Xiao-Hong LI ; Bei-Lei YUN ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
Objective To study the role of Mycoplasma genitalium(Mg)infection in chronic non- bacterial prostatitis.Methods Culture and two PCR systems were performed on prostatic secretion specimens from 487 patients with chronic non-bacterial prostatitis and 75 health men in Changchun regions. Results positive rate of Mg in the prostatic secretion from the 487 patients was 7.39% (36/487);In the controls,the positive rate of Mg was 1.33%(1/75).The differences between the two groups were statistically significant (X~2=3.88,P
6.Application of Flow Cytometry in Viability Detection of Pichia pastoris Cells
An-Feng XIAO ; Xiang-Shan ZHOU ; Li ZHOU ; Yuan-Xing ZHANG ;
Microbiology 1992;0(06):-
Cell viability of Pichia pastoris was detected by flow cytometry (FCM) with two reagents fluorescein diacetate (FDA) and propidium iodide (PI). Compared with FDA/PI double-stained dot plots and PI single-stained dot plots,the latter could divide dead and living cells into two separate zones,and get the correct proportion. Then PI single-stained method was used to detect the change of cell viability in Pichia patoris fermentation. At glycerol batch and fed-batch phase,little dead cells were detected. At methanol fed-batch phase,cell viability decreased when cell weight increased,and was only 73.8% at 88 h.
7.The comparison of fludarabine or antithymocyte globulin based non-myeloablative conditioning regimen for haematologic diseases with graft-versus-host disease.
Qing-Shan LI ; Xiao-Yin ZHUANG ; Ping MAO
Chinese Journal of Hematology 2011;32(8):555-557
Adult
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Antilymphocyte Serum
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therapeutic use
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Female
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Graft vs Host Disease
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prevention & control
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Hematopoietic Stem Cell Mobilization
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methods
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Humans
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Immunosuppressive Agents
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therapeutic use
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Male
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Middle Aged
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Transplantation Conditioning
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methods
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Vidarabine
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analogs & derivatives
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therapeutic use