Amblyopia is a developmental abnormality that results from physiological alterations in the visual cortex and impairs form vision. Based on multimedia, visual perceptual learning was widely applied in amblyopia treatment. Perceptual learning provided an impor-tant new method for treating amblyopia both in children and adults, and it reflects alterations of neural responses in the visual pathway. The therapeutic efficacy of perceptual learning was related to age, amblyopic types and degrees, training time and programs closely.

Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Constipation ; physiopathology ; therapy ; Defecation ; Female ; Humans ; Male ; Middle Aged ; Young Adult

AIDS Vaccines ; immunology ; Animals ; Cytokines ; genetics ; immunology ; Female ; HIV-1 ; immunology ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Vaccines, DNA ; immunology

Objective To evaluate the difference in efficacy and safety between ~(131)Ⅰand antithyroid drugs (ATD)in the treatment of Graves'disease in children and adolescents.Methods MEDLINE(1966-2005), Cochrane Central Register of Controlled Trials(Cochrane Library Issue 2,2006),EMBASE(1984-2004), CBMDISK(1978-2005)and CNKI(1994-2006)were searched by computer.Isotopes(1989-2004),Radiologia Pratica(1986-2005),Chinese Journal of Endocrinology and Metabolism(1985-2004),and Endocrinology and Metabolism Clinics of North America(1988-2001)were manually searched.Trials comparatively analyzed ~(131)Ⅰand antithyroid drugs on the treatment of Graves'disease in children and adolescents were included.The quality of the study methodologies such as randomization,blinding and allocation concealment was evaluated and meta-analysis was performed by Revman 4.2 software.Results Five non-randomized controlled trials involving 538 patients were included.Among these trials one was prospective and the rest were all retrospective.~(131)Ⅰwas more effective in increasing the complete remission and decreasing the rate of recurrence as compared with ATD,but the rate of hypothyroidism was significantly increased after ~(131)Ⅰtreatment.Conclusion Based on the five studies,the evidence suggests that ~(131)Ⅰtherapy is effective and safe for children and adolescents,and the total curative effects in Graves'disease are superior to ATD.However great shortage of randomized controlled trial(RCT),and problems concerning randomization,blind method,follow-up and statistic analysis still exist in clinical controlled trials, hence more RCT with high quality should be conducted.

This paper studied the relationship between apolipoprotein E isoforms and hyperlipidemia among 92 cases of diabetes mellitus, 174 cases with impaired glucose tolerance and 124 sex-age-matched controls. The results demonstrated that the patients with abnormal glucose tolerance had higher frequency of E2/3 isoforms and lower frequency of E, / , in com parison with controls. Hyperlipoproteinemia were mainly related to hyperglycemia but the Apo E isoforms which ex pressed different alleles also associated with hyperlipoproteinemia.

Tegument protein VP22 is encoded by Pseudorabies Virus (PRV) UL49. To identify the nuclear localization signals of UL49, it is necessary to determine the transport mechanism and biological functions of the VP22 protein. In this study, we identified two nuclear localization signals from UL49, NLS1 (5RKTRVA ADETASGARRR21) and NLS2 (241PGRKGKV247). The functional nuclear localization signal (NLS) of UL49 was identified by constructing truncated or site-specific UL49 mutants. The deletion of both NLS1 and NLS2 abrogated UL49 nuclear accumulation, whereas the deletion of NLS1 or NLS2 did not. Therefore, both NLS1 and NLS2 are critical for the nuclear localization of UL49. And our resuts showed that NLS2 is more important in this regard.
Animals ; COS Cells ; Cell Nucleus ; metabolism ; virology ; Cercopithecus aethiops ; Herpesvirus 1, Suid ; chemistry ; genetics ; metabolism ; Humans ; Nuclear Localization Signals ; Protein Transport ; Pseudorabies ; metabolism ; virology ; Viral Structural Proteins ; chemistry ; genetics ; metabolism

OBJECTIVE:To investigate the effect of metformin on sex hormone level,insulin resistance and related indexes in women with polycystic ovarian syndrome(PCOS). METHODS:79 PCOS women were collected as PCOS group,other 86 healthy women as control group. All patients received 0.85 g Metformin tablet,twice a day,for totally 6 months. Boby mass,BMI,waist circumference,hip circumference,ratio of waist to hip,glycated hemoglobin,T,DHEA-S,E2,LH,FSH,PRL,PROG,SHBG, FPG,FINS,LH/FSH,HOMA-IR and FAI before and after treatment in PCOS group were observed and compared with control group. RESULTS:During treatment,2 patients made withdrawal because of pregnancy,3 made withdrawal because of showing gastrointestinal side effects,2 were lost to follow,totally 72 completed it. After treatment,body mass,BMI,waist circumference, hip circumference,glycated hemoglobin,FINS,HOMA-IR,T,LH,LH/FSH,PROG,FAI in PCOS group were significantly low-er than before,SHBG was significantly higher than before,glycated hemoglobin and PROG were lower than control group,while waist circumference,FINS,HOMA-IR,LH/FSH and FAI were higher than control group,the differences were statistically signifi-cant (P<0.05). There was no significant difference in ratio of waist to hip,FPG,DHEA-S,E2,FSH and PRL before and after treatment(P>0.05). CONCLUSIONS:Metformin can effectively improve the sex hormone levels and insulin resistance of patients with PCOS.

Objective To explore the clinical efficacy of dynamic hip screw( DHS) and proximal femoral nail anti-rotation( PFNA) in treatment of patients with Parkinson’ s disease and intertrochanteric fracture. Methods A total of 62 elderly patients of Parkinson’ s disease with femoral intertrochanteric fracture in our hospital from February 2010 to February 2014 were divided into two groups according to different internal fixations,with 31 cases in DHS group and PFNA group respectively. The operation time,X-ray fluoroscopy times,intraoperatve blood soss,the healing time of fracture,postoperative complications and Harris score between two groups were recorded and compared statistically. Results The operation time,intraoperatve blood soss and the clinical healing time of PFNA group were significantly lower than those of DHS group,the differences were statistically significant (P<0. 05). But the times of intraoperative fluoroscopy of PFNA group was more than that of DHS group,the difference was statistically significant (P<0. 05). The Harris score of hip function results showed that the excellent rate of PFNA group was significantly higher than that of DHS group, the difference was statistically significant (P=0. 034). All patients were fol-lowed up for 6 to 48 months. There were 4 cases with complications after operation in DHS group,1 cases of complications in PFNA group,the difference in complications was not significant (P>0. 05). Conclusion The PFNA has the advantages of shorter operation time,less bleed-ing,faster healing time in treatment for elderly patients with Parkinson’ s disease and intertrochanteric fracture,worth clinical promotion.

Objective To investigate the effect of propofol on hypoxic pulmonary vasoconstriction(HPV)and assess the underlying mechanism in rats.Methods Twenty male SD rats weighing 300-400 g were.anesthetizedwith intraperitoneal phenobarbital.Heart and lungs were removed after thoracotomy.Pulmonary.arterial rings 4 mmin length and 1.0-1.4 mm in diameter were prepared and suspended in Earl solution maintained at 37℃ with a pHof 7.40 and aerated with 20% O_2-5% CO_2-25% N_2.The rings were stimulated with phenylephrine(PE)10~(-6)mol?L~(-1) with different preloads(300,500,700,900,1 100 mg).The isometric tension of the arterial rings wasmeasured.The optimal preload was determined to be 900 mg which allowed best contractility.Hypoxia was inducedby aerating the solution with 95% N_2-5% CO_2 and the flow rate was adjusted to maintain PO_2 of the solution at40-20 mm Hg and pH at 7.40.HPV of the rings were recorded.Then propofol was added to achieve a finalconcentration of 1,3,10,30,100 ?mol?L~(-1) and HPV was again induced and the changes in HPV wererecorded.In addition the effects of propofol(10,30 ?mol?L~(-1))on vasoconstriction produced by KCI and PE werealso measured.Results The lower doses of propofol(10 ?mol?L~(-1))significantly inhibited HPV(P

OBJECTIVE:To improve the management of dispensing room in PIVAS in order to provide reference for standardized disposition of drugs.METHODS:The condition of the management of dispensing room of PIVAS in our hospital was introduced in respect of staff management,material management,disposition management,environment and equipment management.RESULTS & CONCLUSIONS:The quality and efficiency of disposition of intravenous drugs are improved through improving management of above four aspects to guarantee the medication safety of patients.