A bacterium which can effectively degrade LAS (Linear Sodium Alkylbenzenesulfonate) was isolated from washing powder manufacturing effluent and was preliminarily identified as Corynebacterium jeikeium GZ6. The bacterium can degrade LAS up to till 700 mg/L, and the optimum pH, temperature and concentration of LAS are 7.0, 30℃ and 400 mg/L, respectively. The biodegradation rate can reach 98.7% after 24 hours'cultivation in the suitable conditions. Experiments also showed that some heavy metal ions such as Hg2+ , Co2+ , Cd2+ can differently inhibit the degradation of LAS.

Objective To explore the safty and feasibility of transrectal ultrasound guided transperineal seminal vesicle biopsy in the evaluation of clinical staging of prostate cancer.Methods Retrospectively study 57 suspected prostate cancer patients with seminal abnormality during 2010.7-2015.1,age ranged from 50 to 78 years,average 65 ±7 years,serum total prostate specific antigen (tPSA) 3.2-131.1 μg/L, average (23.7 ± 11.3) μg/L.Twenty-two cases had palpable prostate nodules through rectal examination.All the 57 patients underwent ultrasound and template guided transperineal prostate and seminal vesicle puncture biopsies.Results Forty-four cases out of 57 found prostate cancer cells in biopsies, and 32 cases had seminal vesicle invasion (positive group) while the other 12 were negative.Twenty cases had been performed prostatectomy in the positive group and their post-operative pathological examination all showed prostate cancer with seminal vesicle invasion.Eleven cases in the negative group had been performed prostatectomy ,and 2 cases showed seminal vesicle invasion.The clinical stages of all cases in the positive group were considered as T3b both pre-operatively and post-operatively.In the negative group however, 11 cases were considered as T2 stage pre-operatively,while 2 cases were increased to T3b stage post-operatively.The sensitivity of puncturing seminal vesicle was 91％ (20/22) ,specificity was 100.0％ (9/9).Positive predictive value was 100.0％ (20/20),while negative predictive value was 82％ (9/11).All the 57 cases did not present fever after puncture biopsies, while 23 cases presented hematuria (40％) ,20 cases presented hemospermia (35％) and 1 case presented urinary retention (2％).Conclusions Transrectal ultrasound-guided transperinealseminal vesicle puncture is safe and reliable, it helps to improve the accuracy of pre-operative staging.

OBJECTIVETo explore the correlation of the gene polymorphisms of Toll-like receptor 2 ( TLR2) and TLR4 with the susceptibility and recurrence of condyloma acuminatum (CA).

METHODSUsing Snapshot, we detected the gene polymorphisms of TLR2 597(T/C), 1350(T/C), 15607(A/G), and 2258(G/A) and TLR4 896(A/G) and 1196(C/T) in the peripheral blood of 140 CA patients and 105 HPV-negative controls. We made comparisons between the CA patients and controls as well as between the cases of recurrent CA and those of non-recurrence at 6 months after treatment.

RESULTSThere were 72, 48, and 20 cases of genotype TT, TC, and CC of TLR2 597 (T/C), respectively, in the CA patients, as compared with 71, 31, and 3 cases in the controls. The gene frequency of mutant C was 31. 43% in the patients, significantly higher than 17.62% in the controls (χ2 = 12.04, P < 0.01), and it was 38.68% in the recurrent cases, remarkably higher than 27.01% in the non-recurrent cases (χ2 = 4.16, P < 0.05). There were 74, 49, and 17 cases of genotype TT, TC, and CC of TLR2 1350( T/C), respectively, in the CA patients, as compared with 73, 29, and 3 cases in the controls. The gene frequency of mutant C was 29. 64% in the patients, significantly higher than 16. 67% in the controls (χ2 =11.05, P < 0.01), and it was 36.79% in the recurrent cases, markedly higher than 25. 29% in the non-recurrent cases (χ2 = 4.18, P < 0.05). There were 44, 66, and 30 cases of genotype AA, AG, and GG of TLR2 15607(A/G), respectively, in the CA patients, as compared with 26, 58, and 21 cases in the controls. There was no significant difference in the gene frequencies of mutant G between the two groups (χ2 = 0.33, P > 0.05). No mutant genes of TLR2 2508 (G/A) or TLR4 896(A/G) and 1196(C/ T) were detected in either the CA patients or the controls. Linkage disequilibrium analysis showed a tight linkage between TLR2 597 (T/C) and 1350(T/C) (D' = 1, r2 = 0.93).

CONCLUSIONTLR2 597(T/C) is tightly linked to 1350(T/C), which is correlated with both the susceptibility and the recurrence of condyloma acuminatum.

Aged ; Case-Control Studies ; Condylomata Acuminata ; genetics ; Gene Frequency ; Genetic Linkage ; Genetic Predisposition to Disease ; Genotype ; Humans ; Polymorphism, Genetic ; Recurrence ; Toll-Like Receptor 2 ; genetics ; Toll-Like Receptor 4 ; genetics

Diagnosis, Differential ; Female ; Humans ; Knee Joint ; pathology ; Middle Aged ; Sarcoma, Synovial ; pathology ; Tomography, X-Ray Computed

Adenocarcinoma, Clear Cell ; Female ; Humans ; Middle Aged ; Nasal Cavity ; Nose Neoplasms

Objectives:To study working memory deficit after stroke.Methods:118 patients with stroke di- agnosed by CT scans(study group)and 118 healthy people(control group)were collected from April to December in 2003.All objects were evaluated with Wechsler Memory Scale(WMS),Trail Making Test A and B,and Wisconsin Card Test(WSCT).Results:There were significant difference between the study group and control group in execu- tive function,attention function,visual recognition and visual recall;The study group had more error in Trail making Test B,A and executive error((?)?SE was 200.0?15.2,76.3?10.4,23.9?0.9)than control group(133.3?7.2, 24.2?1.6,9.9?0.5).Compared with control,patients with stroke of different locations had different deficits in work- ing memory:①for frontal stroke,the deficit manifested in executive function,digital span(P0.05).④for thalamic stroke,the deficit manifested in executive function,digital span,visual recognition and visual recall,(P

Objective To investigate the acute effect of dialysate calcium concentration on calcium balance and blood pressure(BP) of maintenance hemodialysis(MHD) patients with normal serum tCa,and to provide scien- tific basis for individualized calcium concentration dialysate formula.Methods For 4 weeks,dialysate with different calcium concentrations as DCa 1.25,DCa 1.5 and DCa 1.75 was used in 15 stable MHD patients.Arterial blood pressure was measured before and after each dialysis session,and every 30 minutes during hemodialysis session.Serum total calcium and ionized calcium were assessed before and after each dialysis session with different calcium concen- trations dialysate.Results With the DCa 1.2.5,BP,serum tCa and iCa decreased as compared with pre-dialysis val- ues(P

Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal ; metabolism ; Antigens, Neoplasm ; immunology ; metabolism ; Biomarkers, Tumor ; metabolism ; Carcinoma, Squamous Cell ; diagnosis ; metabolism ; pathology ; Cell Cycle Proteins ; immunology ; metabolism ; Cervical Intraepithelial Neoplasia ; diagnosis ; metabolism ; pathology ; Cyclin-Dependent Kinase Inhibitor p16 ; metabolism ; DNA Topoisomerases, Type II ; immunology ; metabolism ; DNA-Binding Proteins ; immunology ; metabolism ; Female ; Humans ; Immunohistochemistry ; Ki-67 Antigen ; metabolism ; Middle Aged ; Minichromosome Maintenance Complex Component 2 ; Nuclear Proteins ; immunology ; metabolism ; Uterine Cervical Neoplasms ; diagnosis ; metabolism ; pathology ; Young Adult

OBJECTIVETo explore the effect of combination of pulsed CO2 laser irradiation and diammine silver fluoride treatment on the ultrastructure of dentine.

METHODSEach extracted molars was made into four identical dentinal specimens, which were randomly divided into 4 groups according to the treatment methods: A, control group; B, pulsed CO2 laser irradiation; C, coated with 38%Ag(NH3)2F; and D, laser irradiation plus fluoride. Scanning electron microscope (SEM) analysis was performed.

RESULTSAs shown by SEM, group A showed clear dentinal tubuli, group B showed melted surface without dentinal tubule openings, group C showed thicker melted layer without dentinal tubule openings, and group D showed the thickest melted and recrystallized layer on dentine surface.

CONCLUSIONSBoth pulsed CO2 laser irradiation and 38%Ag(NH3)2F treatment can seal dentinal tubule. The combination of these two techniques can form a synergistic effect.

Dentin ; drug effects ; radiation effects ; ultrastructure ; Fluorides ; pharmacology ; Humans ; Lasers ; Lasers, Gas ; Silver Compounds ; pharmacology

OBJECTIVETo observe the changes of blood glucose, urinary protein and renal glomerular morphology in rats with diabetic nephropathy, and the effect of xiaoke granule (XKG) on them.

METHODSThe diabetic nephropathy model was established by 3/4 nephrectomy and once intraperitoneal injection of streptozotocin (STZ). The experimental rats were divided into the model group, the XKG group, the positive control group and the sham operation group. Blood was taken from rat's caudal vein to test the fasting blood glucose (FBG) once every week after STZ injection and at the same time, urinary protein in 24 hrs (UP/24h) was investigated. All the rats were sacrificed 2, 6 weeks after STZ injection and morphological examination on their kidney was performed.

RESULTSSix weeks after STZ injection, glomerular sclerosis in various degrees was seen in the model group, but the pathological change was significantly milder in the treated groups. FBG in the model group was higher than that in the sham operation group at all time points respectively (P<0.05), while in the XKG group and the positive control group, the change was improved significantly. UP/24h in the model group was higher than that in the sham operation group at all time points respectively, and that in the XKG group at 1, 2 and 3 weeks after STZ injection was significantly lower than that in the model group. FBG and UP/24h showed a positive correlation.

CONCLUSIONA rat model of diabetic nephropathy was duplicated successfully. The elevated blood glucose level in diabetic nephropathy model could induce proteinuria. One of the routes of treatment of diabetic nephropathy by XKG is to reduce the blood glucose, eliminate the proteinuria, and thus to improve the pathological change in renal glomeruli.

Animals ; Blood Glucose ; metabolism ; Diabetes Mellitus, Experimental ; blood ; pathology ; Diabetic Nephropathies ; blood ; etiology ; pathology ; Drugs, Chinese Herbal ; pharmacology ; Hypoglycemic Agents ; pharmacology ; Kidney Glomerulus ; pathology ; Male ; Nephrectomy ; Proteinuria ; urine ; Rats ; Rats, Sprague-Dawley