Purpose:To evaluate the effects of intra-arterial infusion plus radiation therapy as a treatment for invasive bladder cancer. Methods:24 cases of invasive bladder cancer were treated from 1995 to 2003. All were treated with intra-arterial infusion chemotherapy plus radiation therapy. Results:CR+PR was 91.6%(22?24). In the 22 cases with indications for total cystectomy, the normal bladder function was preserved. The quality of life and the survival period improved. Conclusions:Intra-arterial infusion chemotherapy plus radiation therapy is an effective method for invasive bladder cancer, especially for the patients who can not tolerate burden or do not accept cystectomy.[

Adolescent ; Child ; Diabetes Mellitus ; drug therapy ; Drug Design ; Humans ; Hypoglycemic Agents ; administration & dosage ; therapeutic use ; Insulin ; administration & dosage ; therapeutic use

Adolescent ; Child ; Diabetes Mellitus ; drug therapy ; Humans ; Hypoglycemic Agents ; pharmacokinetics ; therapeutic use ; Insulin, Short-Acting ; pharmacokinetics ; therapeutic use

3D printing technology has become the focus of the study on rapid proyotyping technology in recent years. This paper introduced the principle, material and equipments of 3D printing, as well as the foot orthosis. This paper also summarized the application of 3D printing in custom-made foot orthosis, and the differences between 3D printing and traditional technology from the aspect of structure design, material, and clinical application, etc., and discussed the problems and future development of 3D printing.

Animals ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Central Nervous System ; drug effects ; growth & development ; Female ; Organophosphorus Compounds ; toxicity ; Pesticides ; toxicity ; Pregnancy ; Rats

Objective To investigate the correlation between the expression of human leukocyte antigen B27(HLA-B27)antigen and ankylosing spondylitis(AS),and its implications for the diagnosis and differential diagnosis of AS.Methods Flow cytometry was used to detect HLA-B27.354 suspected AS patients,35 patients final diagnosed with AS and 30 patients diagnosed with rheu-matoid arthritis(RA)were enrolled in the study.Results The positive rate of HLA-B27 in 354 patients with suspected AS was 22.0%,in patients diagnosed with AS was 85.7%,which was higher than the RA group(P <0.05).In AS patients,Males accoun-ted for 77.1%,females accounted for 22.9%,and the patients′age mainly concentrated in youth.The sensitivity and specificity of HLA-B27 test was 85.7% and 100.0% respectively in the diagnosis of AS.Conclusion HLA-B27 antigen detection is important for early diagnosis and differential diagnosis of AS patients.

Objective To investigate the effect of different stimulating culture conditions and detecting methods on the frequency of Th17 cells and to set up and optimize the experimental method for detecting the frequencies of human Th 17 cells in peripheral blood by flow cytometry (FCM ) .Methods The peripheral blood in the healthy people was performed the stimulating culture by phorbol myristate acetate (PMA) at different final concentrations(50 ,100 ,150 ,200 ,250 ,300 ng/mL) and ionomycin 1 μg/mL .The frequencies of Th17 cells were assessed by FCM after different stimulating time points (4 ,6 ,8 ,10 ,12 h) .The mean fluorescence in-tensity(MFI) and percentage of the ratio of CD4 positive cells on the cellular surface and inside cell membrane were measured be-fore and after PMA stimulation .Results The frequency of Th17 cells was highest with the good stimulation effect under the condi-tion of PMA 150 ng/mL and ionomycin1μg/mL stimulation for 6 h (P<0 .05) .MFI of CD4 inside cellular membrane after stimula-tion was lower than that in the cells with stimulation after labeling membrane surface CD 4(P<0 .05) ,and the Th17 cell frequencies and the ratio of CD4 positive cells were higher than those in the latter ,but the differences were not statistically significant (P>0 .05) .Conclusion After stimulating whole blood with the final concentration of PMA 150ng/mL and ionomycin1μg/mL for 6 h , measuring intracytoplasm CD4 and IL-17 levels is the simple and effective method for detecting the frequency of human Th 17 cells .

Objective: To investigate the changes of cholesterol 7alpha- hydroxylase(CYP7A1) ,farnesoid X receptor(FXR), and small heterodimer partner (SHP) expression in liver tissues of bile duct ligated (BDL) rats,and to analyze the relationship between their expression. Methods: Obstructive cholestasis rat model was induced by bile duct ligation,and rats were sacrificed on day 1,3,and 14 after operation. The hepatic tissues were collected and the total mRNA was extracted. The CYP7A1 mRNA expression was determined by real-time RT-PCR. The protein expression of FXR and SHP in the nuclei was determined by Western blotting analysis. Results: Compared with sham-operated group, experimental obstructive cholestasis group had significantly enhanced CYP7A1 mRNA expression and decreased FXR,SHP expression(P<0. 001,P<0. 05). Conclusion: The up-regulation of CYP7A1 may be associated with the down-regulation of FXR and SHP.

Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Arthralgia ; therapy ; Female ; Humans ; Male ; Middle Aged ; Needles

The effects of continuous subcutaneous insulin injection (CSII, n=306) and multiple subcutaneous insulin injection (MSII, n=310)on hyperglycemia in patients with diabetes mellitus were compared. The results suggest that the effect of CSII is more prompt and efficient with lower incidence of hypoglycemia as compared with MSII.