1.Value of Friedman clinical staging systems in management with uvulopalatopharyngoplasty for obstructive sleep apnea hypopnea syndrome.
Pei-Jie HE ; Kuan-Lin XIAO ; Fang-Lu CHI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(2):154-155
Adult
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Female
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Humans
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Male
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Middle Aged
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Palatine Tonsil
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pathology
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Sleep Apnea, Obstructive
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pathology
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surgery
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Tongue
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pathology
2.Analysis of current trauma epidemiology in Zhengzhou during 2004-2006
Bao-Chi LIU ; Hui PEI ; Wei-Xin HU ; Xiao-Qing KANG ;
Chinese Journal of Emergency Medicine 2006;0(10):-
Objective To investigate the present status of casualty epidemiology in Zhengzhou and the effects of different trauma care model.Method Statistic study of the classification of emergency disease and the number of ambulance responses to the call in Zhengzhou emergency rescue center from January,2004 through December 2006 was carried out and the efficiency between the trauma care model of an inclusive emergency rescue survices station and that of a simple emergency survices station was analyzed.Results The percentage of ambulance departure responses by Zbengzhou emergency rescue center for trauma care was 45.3 %,44.7 % and 45.8% of in 2004,2005 and 2006,respectively.There were 26 emergency rescue service stationa in Zhengzhou, including one independent station,eight internal medicine dominated semi-independent stations and seventeen simple model stations.The indicated surgical intervention can lye performed on the patients with severe multi- trauma in the independent emergency station in order to win the optimal operation time and reduce the mortality. Conclusions The trauma is the major reason for the emergency call.Emergency rescue service stations properly distrihtted,can offer quick and efficient pro-hospital first aid.The independent emergency rescue service station can increase successful resuscitation rate of serious casualties.
3.Identification of the bacteria strain and its antibiotic resistance in an epidemic of cholera
Xiao-hong ZHOU ; Pei-hua XU ; Yan-jing NI ; Jian CHI ; Xiao-wei ZHU
Shanghai Journal of Preventive Medicine 2021;33(2):128-
Objective The present study was conducted to identify the
4.Cytotoxic constituents from the leaves of Broussonetia papyrifera.
Xiao-Ku RAN ; Xiao-Tong WANG ; Pei-Pei LIU ; Yu-Xin CHI ; Bo-Jia WANG ; De-Qiang DOU ; Ting-Guo KANG ; Wei XIONG
Chinese Journal of Natural Medicines (English Ed.) 2013;11(3):269-273
AIM:
To investigate the chemical constituents from the leaves of Broussonetia papyrifera.
METHODS:
The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography and preparative HPLC. Their structures were elucidated on the basis of 1D and 2D NMR analyses. In addition, their cytotoxic activity against human hepatoma carcinoma cells (HepG-2) were evaluated by the MTT method. Furthermore, RP-HPLC and colorimetric methods were used for the analysis of cosmosiin and total flavonoids.
RESULTS:
A new lignan, together with five known compounds were obtained, and their structures were characterized as (+)-pinoresinol-4'-O-β-D-glucopyranosyl-4″-O-β-D-apiofuranoside (1), cosmosiin (2), luteolin-7-O-β-D-glucopyranoside (3), liriodendrin (4), 3, 5, 4'-trihydroxy-bibenzyl-3-O-β-D-glucoside (5), and apigenin-6-C-β-D-glucopyranside (6). Furthermore, RP-HPLC and colorimetric methods were established for the analysis of cosmosiin and total flavonoids.
CONCLUSION
Compound 1 was a new lignan, and compounds 5 and 6 were isolated for the first time from the title plant. Compounds 1, 4 and 6 showed definite activities against HepG-2, while the other compounds didn't show inhibitory effects. The optimal harvest time of B. papyrifera (L.) Vent. is September.
Broussonetia
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chemistry
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Cell Proliferation
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drug effects
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Cytotoxins
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chemistry
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isolation & purification
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toxicity
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Hep G2 Cells
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Humans
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Lignans
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chemistry
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toxicity
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Molecular Structure
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Plant Extracts
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chemistry
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isolation & purification
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toxicity
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Plant Leaves
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chemistry
5.Clinical observation on treatment of hyperplasia of mammary gland by Lirukang Granule.
Li-qi QIAN ; Xiao-hua PEI ; Zhi-yin XU ; Chi WANG
Chinese journal of integrative medicine 2007;13(2):120-124
OBJECTIVETo explore the efficacy and mechanism of Lirukang Granule in treating hyperplasia of mammary gland (HMG).
METHODSOne hundred patients with HMG were randomly assigned to two groups, 50 in each group. The patients in the treated group were orally administered with LRKG thrice a day, one package each time, and those in the control group were given orally Rukuaixiao Tablet thrice a day, 4 tablets each time. The therapeutic course for both groups was 4 months. The clinical efficacy, pain alleviating rate, as well as changes of local sign and symptom scores were observed before and after treatment. The changes of serum estradiol (E(2)), progesterone (P), testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH) and prolactin (PRL) in some randomly selected patients (24 patients in the treated group and 24 in the control group) before and after treatment were measured with radioimmunoassay.
RESULTSThe total clinical efficacy in the treated group was superior to that in the control group, significant difference was shown between the two groups (P < 0.01). The cure-effective rate and total effective rate in the treated group were 70.0% and 88.0% respectively, significantly higher than those in the control group (38.0% and 64.0%) respectively (P < 0.01), and the pain alleviating rate in the treated group was also significantly higher in the former than that in the latter (88.0% vs 64.0%, P < 0.05). Moreover, the treated group showed obvious superiority in improving the patients' symptom and sign scores (P < 0.01), and abnormalities of gonadal hormone as compared with the respective items in the control group (P < 0.01).
CONCLUSIONLRKG has good efficacy in the treatment of HMG, and its mechanism may be related to the regulation on endocrine and immune function.
Adult ; Breast ; pathology ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Follicle Stimulating Hormone ; blood ; Humans ; Hyperplasia ; Luteinizing Hormone ; blood ; Middle Aged
6.Evaluation of Chai Shao Liu Jun Tang for the treatment of chronic hepatitis B.
Xiao-ling CHI ; Li-ming WU ; Jun-min JIANG ; Pei-qiong CHEN ; Guang-jun TIAN ; Huan-ming XIAO ; Gao-shu CAI ; Ying CHEN ; Ying QIAN
Chinese Journal of Hepatology 2009;17(6):440-442
OBJECTIVETo evaluate the effect of Chai Shao Liu Jun Tang in combination with Lamivudine for the treatment chronic hepatitis B (CHB) patients.
METHODS405 CHB patients in Guangdong Provincial Hospital were randomly divided into 2 groups, 220 in the treated group, and 185 in the control group. The control group was treated with Lamivudine for 18 months. The treated group was treated with Lamivudine in combination with Chai Shao Liu Jun Tang for 18months. At the 3rd, 6th, 9th, 12th and 18th month during the treatment, the clinical symptoms, ALT normalization rate, HBeAg seroconversion rate, the proportion of patients with undetectable serum HBV DNA, and YMDD mutation rate were observed.
RESULTSALT normalization rates at the 3rd, 6th, 12th, 18th month of the treatment group (69.5%, 85.9%, 90.5%, 82.7%) were higher than those in the control group (50.3%, 65.4%, 78.4%, 69.7%; P < 0.01). HBeAg seroconversion rate, rate of HBV DNA undetectable, and YMDD mutation rate at he 12th and18th month are 77.7%, 57.7%, 25.5%, 6.8%; 86.8%, 74.1%, 33.2%, 8.6% in the treatment group, and 54.6%, 36.8%, 13.0%, 14.6%; 69.2%, 37.3%, 19.5%, 20.5% in the control group (P < 0.01, or P < 0.05).
CONCLUSIONCompared to lamivudine alone, Cai Shao Liu Ju Tang in combination with lamivudine is more effective and induces less YMDD mutation rate in CHB patients.
Adult ; Alanine Transaminase ; blood ; DNA, Viral ; blood ; genetics ; Drug Combinations ; Drug Resistance, Viral ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Genes, Viral ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; drug effects ; genetics ; Hepatitis B, Chronic ; blood ; drug therapy ; virology ; Humans ; Lamivudine ; administration & dosage ; therapeutic use ; Liver Function Tests ; Male ; Medicine, Chinese Traditional ; Mutation ; Phytotherapy ; Treatment Outcome ; Virus Replication ; drug effects
7.Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells.
Jin WANG ; Fang-ting HE ; Chi-hung TZANG ; Wang-fan FONG ; Pei-gen XIAO ; Rui HAN ; Meng-su YANG
Acta Pharmaceutica Sinica 2005;40(12):1099-1104
AIMTo elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel.
METHODSFlow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9984 genes and expressed sequence tags (ESTs).
RESULTSCell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol x L(-1) paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol x L(-1) (IC50) and 100 nmol x L(-1) paclitaxel treatment, respectively.
CONCLUSIONPaclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.
Antineoplastic Agents, Phytogenic ; administration & dosage ; pharmacology ; Apoptosis ; drug effects ; Breast Neoplasms ; genetics ; pathology ; Cell Cycle ; drug effects ; Cell Line, Tumor ; DNA Repair ; drug effects ; Dose-Response Relationship, Drug ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Oligonucleotide Array Sequence Analysis ; Paclitaxel ; administration & dosage ; pharmacology
8.Inhibition of Adhesion and Metastasis of HepG2 Hepatocellular Carcinoma Cells In Vitro by DNA Aptamer against Sialyl Lewis X
WANG XIAO-KANG ; PENG YAN ; TAO HAO-RAN ; ZHOU FEN-FANG ; ZHANG CHI ; SU FEI ; WANG SHI-PEI ; LIU QING ; XU LI-HUA ; PAN XUE-KAI ; XIE WEI ; FENG MAO-HUI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(3):343-347
The sialyl Lewis X (SLex) antigen encoded by the FUT7 gene is the ligand of endotheliam-selectin (E-selectin).The combination of SLex antigen and E-selectin represents an important way for malignant tumor metastasis.In the present study,the effect of the SLeX-binding DNA aptamer on the adhesion and metastasis of hepatocellular carcinoma HepG2 cells in vitro was investigated.Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence staining were conducted to detect the expression of FUT7 at both transcriptional and translational levels.The SLex expression in HepG2 cells treated with different concentrations of SLeX-binding DNA aptamer was detected by flow cytometry.Besides,the adhesion,migration,and invasion of HepG2 cells were measured by cell adhesion assay,and the Transwell migration and invasion assay.The results showed that the FUT7 expression was up-regulated at both mRNA and protein levels in HepG2 cells.SLeX-binding DNA aptamer could significantly decrease the expression of SLex in HepG2 cells.The cell adhesion assay revealed that the SLeX-binding DNA aptamer could effectively inhibit the interactions between E-selectin and SLex in the HepG2 cells.Additionally,SLeX-binding DNA aptamers at 20 nmol/L were found to have the similar effect to the monoclonal antibody CSLEX-1.The Transwell migration and invasion assay revealed that the number of penetrating cells on the down-side of Transwell membrane was significantly less in cells treated with 5,10,20 nmol/L SLeX-binding DNA aptamer than those in the negative control group (P<0.01).Our study demonstrated that the SLeX-binding DNA aptamer could significantly inhibit the in vitro adhesion,migration,and invasion of HepG2 cells,suggesting that the SLeX-binding DNA aptamer may be used as a potential molecular targeted drug against metastatic hepatocellular carcinoma.
9.Knockdown of GRHL3 Inhibits Activities and Induces Cell Cycle Arrest and Apoptosis of Human Colorectal Cancer Cells
WANG XIAO-KANG ; ZHOU FEN-FANG ; TAO HAO-RAN ; WANG XIN ; ZHANG CHI ; SU FEI ; WANG SHI-PEI ; XU LI-HUA ; PAN XUE-KAI ; FENG MAO-HUI ; XIE WEI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(6):880-885
The Grainyhead-like 3 (GRHL3) is involved in epidermal barrier formation,neural tube closure and wound repair.Previous studies have suggested that GRHL3 has been linked to many different types of cancers.However,to date,its effects on human colorectal cancer (CRC) has not been clarified yet.Our microarray analysis has indicated predominant GRHL3 expression in CRC.The purpose of this study was to investigate the expression and significance of GRHL3 in CRC tumorigenesis using CRC tissues and paired paracancerous tissues,as well as using distinct CRC cell lines (HT29 and DLD1).We observed increased GRHL3 expression at both mRNA and protein levels in CRC tissues and CRC cell lines using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting.Moreover,silencing GRHL3 with siRNA could suppress CRC cell proliferation,viability and migration in vitro.We also found that knockdown of GRHL3 could promote cell cycle arrest at G0/G1 phase in HT29 cells and DLD1 cells,and induce cell apoptosis in HT29 cells.Together,our study revealed the down-regulation of GRHL3 in vitro could inhibit CRC cell activity and trigger cell cycle arrest at G0/G1 phase and apoptosis.
10.Molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans retinoic acid.
Jin WANG ; Chi-hung TZENG ; Ming-hui HUANG ; Hong-xun FANG ; Pei-gen XIAO ; Rui HAN ; Meng-su YANG
Acta Pharmaceutica Sinica 2004;39(1):22-28
AIMTo elucidate the molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA).
METHODSFlow cytometry was used to determine the cell cycle changes of HL-60 cells upon ATRA treatment. Gene expression profiles of HL-60 cells induced by 1 mumol.L-1 ATRA were obtained by using cDNA microarrays containing 9,984 genes and expressed sequence tags (ESTs).
RESULTSCell cycle analysis showed that 48%-73% of cells were arrested at G1/G0 phase upon ATRA treatment; cDNA microarray results demonstrated that the expression of genes encoding adhesion molecules, tissue remodeling proteins, transporters and ribosomal proteins were up-regulated in ATRA treated of HL-60 cells. Several genes involved in oxidase activation pathway were also differentially expressed.
CONCLUSIONATRA treatment induced growth arrest and differentiation in HL-60 cells, which is associated with regulation of the oxidase activation pathway and the expression of tissue remodeling proteins.
Antineoplastic Agents ; pharmacology ; Cell Cycle ; Cell Differentiation ; Gene Expression Profiling ; Granulocytes ; drug effects ; pathology ; HL-60 Cells ; Humans ; Oligonucleotide Array Sequence Analysis ; Tretinoin ; pharmacology