OBJECTIVETo measure the changes of plantar pressure of the first tarsometatarsal joint fracture and dislocation by three different implants to provide experimental reference in selecting implants.

METHODSEight fresh foot specimens were made into the models of the first tarsometatarsal joint fracture and dislocation, which were fixed with 3.5 mm cortical screw, 1/4 tubular plate and compressive staple in turn. After the loading of 600 N, the changes of the plantar pressure in forefoot were measured by the method of the F-scan plantar pressure system.

RESULTSAfter first tarsometatarsal joint fracture and dislocation, the peak pressure under the first metatarsal head would decrease, while the pressure under the second metatarsal head would increased,whose differences were statistically significant (P<0.05). When the first tarsometatarsal joint was fixed with screw or plate respectively; the peak pressure under the two metatarsal heads would tend to be normal. However,the staple fixation showed the statistical significant difference compared with normal state, although the peak pressure under the first and second metatarsal heads were recovered in some extent(P<0.05).

CONCLUSIONAfter the first tarsometatarsal joint fracture-dislocation, the plantar pressure might be compensated partly by the adjacent metatarsal heads according to the regulation of the load transfer mechanism. While the first tarsometatarsal joint fracture-dislocation was fixated by screw or plate, the plantar pressure of the forefoot would return to the normal state. However,if the joint was fixated by the staple, it would still be difficult to return the plantar pressure to be normal.

Foot Joints ; injuries ; surgery ; Forefoot, Human ; physiology ; Fracture Fixation, Internal ; methods ; Fractures, Bone ; surgery ; Humans ; Joint Dislocations ; surgery ; Pressure

In the study of T2DM(n=30),IGR(n=26) and NGT(n=30) cases in Guangxi Province,T2DM had higher resistin and PAI-1 levels than did the groups of IGR and NGT(P<0.05).IGR had higher levels of PAI-1 and leptin than NGT(P<0.05).Leptin level of T2DM was higher than that of NGT(P<0.05).Insulin resistance index was positively correlated with leptin,TG and PAI-1 levels(P<0.05).

In order to study the variation of complex impedance and characteristic parameters on human normal and tumor lung tissue during the extracorporeal time, we established a real part-imaginary part chart of complex impedance on lung tissue which provided the basic theory and the reference data for research on elementary medicine and clinical diagnosis of lung cancer and meanwhile provided prior information for electrical impedance tomography (EIT) research. In the experiment carried out in our laboratory, when operation was finished, we kept the lung cancer tissue and normal tissue neatly separated into the cylindrical testing cavities and kept the temperature and humidity at expected values. Then the measurements of complex impedance property are performed at frequency from 1 000 Hz to 30 MHz using 4294A impedance analyzer of Aglient Company. With time changing, the results showed that there was a significant change occurring on the complex impedance of human normal and tumor lung tissue. However, the impedance of normal lung tissue is greater than that of tumor lung tissue. We consider that this change should be related to the change in extracellular fluid, intracellular fluid and cell membrane.
Electric Impedance ; Humans ; Lung ; physiology ; Lung Neoplasms ; pathology ; Tomography

This paper aimed to investigate the botanical origins of Isatidis Radix and Isatidis Folium, and clarify the confusion of its classification. The second internal transcribed spacer (ITS2) of ribosomal DNA, the chloroplast matK gene of 22 samples from some major production areas were amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. Phylogenetic study was performed using MEGA 4.0 software in accordance with the Kimura 2-Parameter (K2P) model, and the phylogenetic tree was constructed using the neighbor-joining methods. The results showed that the length of ITS2 sequence of the botanical origins of Isatidis Radix and Isatidis Folium was 191 bp. The sequence showed that some samples had several SNP sites, and some samples had heterozygosis sites. In the NJ tree, based on ITS2 sequence, the studied samples were separated into two groups, and one of them was gathered with Isatis tinctoria L. The studied samples also were divided into two groups obviously based on the chloroplast matK gene. In conclusion, our results support that the botanical origins of Isatidis Radix and Isatidis Folium are Isatis indigotica Fortune, and Isatis indigotica and Isatis tinctoria are two distinct species. This study doesn't support the opinion about the combination of these two species in Flora of China.
Chloroplasts ; genetics ; DNA Barcoding, Taxonomic ; methods ; DNA, Plant ; genetics ; DNA, Ribosomal Spacer ; genetics ; Genes, Plant ; genetics ; Isatis ; classification ; genetics ; Phylogeny ; Plant Leaves ; genetics ; Plants, Medicinal ; classification ; genetics ; Species Specificity

Objective To explore the application of self-expanding stent and seperable detachable coil for interventional therapy in patients with wide-necked intracranial aneurysm.Methods 32 patients with wide-necked intracranial aneurysm received the interventional therapy by self-expanding stent and seperable detachable coil.After operation,the situation of the aneurysm occluded were observed by DSA immediately and followed up.Results The stents were successfully deployed in the 32 patients.The seperable detachable coils were occluded in the aneurysms in 29 cases.After operation immediately,the DSA showed that the aneurysms in 18 cases were completely occluded by seperable detachable coils,7 cases were 95% occluded,4 cases were 90% occluded.In the other 3 cases,the aneurysms were too small to be filled by seperable detachable coil,the stents were used only.All the parent arteries were no stenosis.18 h after operation,1 case died from multiple hemorrhage caused by thrombasthenia.Follow-up from 3 months to 1 year,DSA re-examination in 21 cases.Compared with the image immediately after operation,the imagings of DSA were no change in 12 cases,residual necks were disappeared in 5 cases and increased in 1 case.In the 3 cases which stents used only,the aneurysms were no development completely in the 2 cases and decreased in 1 case.6～18 months followed up in 26 cases,the rebleeding and cerebral infarction were no occurred.Conclusions The self-expanding stent is very flexible,secure,and effective.It can good support for the wide-necked aneurysms to be occluded by seperable detachable coils and effective promoting the small aneurysms healing.

BACKGROUND:At present, isokinetic testing system has attracted more and more attentions in the evaluation of athlete muscle function, but its application in the detection of waist and abdomen muscle strength characteristics in juvenile male basketbal athletes is rarely reported. OBJECTIVE:To study the biomechanical characteristics of waist flexor and extensor muscles inoutstanding juvenile male basketbal athletes in order to provide the basis for waist and abdominal strength training, scientific selection and prevention of lumbar abdomen injury in basketbal sport. METHODS:Eighteen athletes from the Guangzhou Men’s Basketbal Team were subjected to the detection of muscle strength and work of waist flexor and extensor muscles at 60(°)/s and 180(°)/s using Cybex-Norm isokinetic test system. RESULTS AND CONCLUSION:At the same testing speed, the peak torque and relative peak torque of the extensor muscles were higher than those of the flexor muscles (P<0.01). The increase in the peak torque, relative peak torque and total work of the waist extensor and flexor muscles exhibited a reduced tendency with the increasing of testing speed (P<0.05);the peak torque and relative peak torque of the extensor muscles were decreased more significantly (P<0.01);the relative power of the waist extensor and flexor muscles were increased gradual y with the increasing of testing speed (P<0.01). These parameters were better in perimeter players than in post players. Under the isokinetic concentric contraction, the peak torque ratio of extensor to flexor muscles showed a decreasing trend with the increasing of testing speed and the waist flexor peak torque and extensor muscles flexion showed a decreasing trend, and the trunk stability was weakened. These findings indicate that the muscle strength of the extensor muscles was higher than that of the flexor muscles;the muscles strength of the waist flexor and extensor muscles was better in perimeter players than in post players. In isokinetic rapid movement, the muscle strength of the waist flexor and extensor muscles were reduced and the balance of the muscle strength of the waist flexor and extensor muscles was weakened, suggesting rapid strength training of the waist and abdominal core muscles should be strengthened in juvenile male basketbal athletes.

Objective To evaluate the value of combined application of computed tomography perfusion imaging(CTPI)and CT angiography(CTA) in the diagnosis of hyperacute ischemic cerebrovascular disease.Methods 46 patients with hyperacute ischemic cerebrovascular disease(onset within 6 hours) were examined by conventional CT , CTPI and CTA.Results Conventional CT scan showed low density signal in 5 of 46 patients. CTPI revealed normal perfusion in 16 patients and hypoperfusion in 30 patients. 27 patients existed stenosis or occlusion of middle cerebral arteries(MCA) and anterior cerebral arteries(ACA) by CTA. CTA also showed obvious slight MCA in 2 cases and normal image in 17 cases. 25 cases with large or moderate cerebral infarction volume were confirmed by CTPI as having ischemic hypoperfusion lesions and stenosis or occlusion of arteries by CTA. In 9 cases with small cerebral infarction volume, CTPI revealed perfusion defect in 5 patients and CTA displayed stenosed arteries in 2 patients. CTPI of 12 patients with TIA were normal. But CTA showed remarkable slight MCA and more blood vessel network in its distal segment in 2 of 12 patients.Conclusion combined application of CTPI and CTA can detect hyperacute ischemic lesion at far more early stage. They are helpful in differentiation TIA from infarcts with different volumes.

Objective Calcineurin-NFAT pathway in the regulation of VSMCs proliferation induced by catecholamines.Methods Primary VSMCs from rat aorta were used as the experimental model.Proliferation of VSMCs was measured by MTT assay and cell count.Calcineurin protein and its activity were assayed with immunoblotting and free inorganic phosphate content analysis respectively.Localization of NFATc1 was detected by immunofluorescence staining.Results Phenylephrine(PE,an ?1-adrenoceptor agonist) increased VSMCs proliferation.Prazosin(an ?1-adrenoceptor antagonist),cyclosporin A(CsA,an inhibitor of calcineurin) and chelerythrine(an inhibitor of PKC) decreased PE-induced absorbance and cell number.Timolol(?-adrenoceptor antagonist) has no effect on absorbance and cell number induced by PE.Additional treatment with CsA further inhibited PE-induced absorbance and cell number compared with the chelerythrine pretreatment group.CsA and chelerythrine alone had no significant effect on either absorbance or cell number.CsA decresed PE-induced alcineurin level and its activity.NFATc1 was translocated from cytoplasm to nucleus upon treatment with PE.This translocation was reversed by CsA.Conclusion CsA partially inhibits PE-induced VSMCs proliferation via inhibiting calcineurin activity and NFATc1 nuclear translocation.Calcineurin-NFATc1 pathway is involved in hyperplastic growth of VSMCs induced by catecholamines.

Objective To study the outcome of ureterocele trated by open surgery and by endoscopic manipulation. Methods We reviewed 29 cases of ureteroceles, including 16 intravesical ureteroceles and 13 extravesical ureteroceles. 19 cases were treated by open surgery and 10 by endoscopic procedure. Results 11 cases of intravesical ureteroceles and 8 cases of extravesical ureteroceles under went open surgery, The reoperation rate was 18.1% and 12.5%, respectively. 5 cases of intravesical ureteroceles and 5 cases of extravesical ureteroceles underwent endoscopic treatment,the reoperation rate being 40.0% and 80.0% respectively. Conclusions Endoscopic approach might be the primary management for intravesical ureteroceles, but open surgery is a favorable alternative for extravesical ureteroceles.

Objective To investigate the application of computed tomography perfusion imaging(CTPI)in hyperacute ischemic cerebrovascular disease. Methods Conventional computed tomography (CT) and CTPI were performed within 6 hours of symptom onset in 46 patients with cerebrovascular disease. Results In 46 cases,5 cases diagnosed as cerebral infarction obtained regular CT positive results.In 41 having regular CT negative results,29 had cerebral infarction,and the other 12 had transient ischemic attack(TIA).In 16 cases with CTPI negtive,12 were diagnosed as having TIA and the others having small infarction volume.30 cases were confirmed by CTPI as having ischemic lesions.In 30 cases,5 had ischemic lesions larger in CTPI than in regular CT and 25 had regular CT negtive results.The regional cerebral blood flow(rCBF),time to peak(TP),mean transit time(MTT)in 30 cases with CTPI positive were found obviously changed.The side of ischemic lesion as compared with the opposite side,and the core of ischemic lesion as compared with peripheral zone were found changed significantly( P