For the purpose of preventing and treating the injuries in female football players, we investigated the incidence and the types of injuries, and analyzed the causes that lead to the injuries in 175 players from 8 female football teams. The results were as follows: (1) The incidence of injuries was 235.4 ％, including 41.8 ％ of acute and 58.2 ％ of chronic injuries. (2) The main injures included contusion (46.5 ％), sprain (22.8 ％), muscle torsion (11.5 ％), and abrasion (10.9 ％). (3) The injuries usually occurred in ankle (22％), knee (14.8 ％), thigh (14 ％), head and neck (9.5 ％), leg (8.5 ％), foot (7.4 ％), lumbar (6.6 ％). (4) The major causes of injuries included: inadequate training (33.3 ％), disobeying training principles (25.2 ％), man-made factors (24.2 ％),and others (17.5 ％). In accordance with the causes, 93 players from Guangdong, Henan, Shandong, Bayi female football teams in 1997 were taken as the treatment group with various prevention and treatment measures, while 58 players from Beijing, Hebei, Shanxi female football teams were taken as the control group. The incidence of injury in 1998 was 105.4 ％ in the treatment group, while 229.3 ％ in the control group (P<0.05). As compared with the incidence of injury in 1997 (235.4 ％), the incidence of injury in the treatment group was also statistically lower (P<0.05). It was concluded that the measures of prevention and treatment in the study were effective.

Objective: To observe the in vitro and in vivo antifungal activity of a triadimenol compound SL-130. Methods: The M27-A project recommended by the National Committee for Clinical Laboratory Standard (NCCLS) in 1997 was used in the present study. The minimum inhibitory concentrations (MIC80) of SL-130 were determined for candidal and non-candidal strains with different susceptibilities to fluconazole. Agar disk diffusion test, time-kill curves test and mouse survival rate were employed to examine the antifungal activity of SL-130. Results: The results indicated that SL-130 had stronger in vitro antifungal activity than fluconazole, and similar in vivo activity to fluconazole. Conclusion: The structure of SL-130 is quite unique and it has in vitro and in vivo antifungal activity: further study is warranted on the compound.

Objective: To compare the effects of combination of TAM (tamoxifen) and ADR (doxorubicin) with the respective effect of TAM or ADR alone on the growth of human osteosarcoma MG-63 cells. Methods: The mRNA expression of ER (estrogen receptor) in MG-63 cells was detected by RT-PCR (reverse transcription-PCR). The morphological changes of MG-63 cells treated with TAM or ADR alone as well as the combination of them were observed under an inverted microscope, and the cell viability was tested by MTT colorimetric assay. Results: The expressions of ERα and ERβ were found in MG-63 cells. The typical apoptotic change of MG-63 cells was observed in each drug intervention group but not occurred in the control group without drug intervention. MTT colorimetric analysis demonstrated that the proliferation inhibition rate of MG-63 cells in the combination group was significantly higher (even two-fold higher) than those in the single-drug groups (with the same dose or double dose) (P < 0.05). Conclusion: ERα and ERβ are expressed in human osteosarcoma MG-63 cells. Both TAM and ADR alone can inhibit the proliferation of MG-63 cells, and the combination of the two can present more obviously inhibitory effect. One of the possible underlying mechanisms might be that TAM can enhance the sensitivity of MG-63 cells to ADR. Copyright © 2013 by TUMOR.

Objective To study the expression and the role of interleukin (IL)-23/IL-17 axis in colonic tissue of trinitrobenzene sulphonic acid (TNBS) induced eolitic mice.Methods Mice were divided into four groups:control (n=24),TNBS (n= 24),TNBS 48 hours + mlL-17 antibodies (n=24),TNBS 48 hours + normal rat blood-serum (n= 24).TNBS-induced colitis model was constructed.The mice in control group and TNBS colitis group were sacrificed at 24 hours,48 hours,7th day,respectively.In TNBS 48 hours + mlL-17 antibodies group and TNBS 48 hours + normal rat blood-serum group,a single injection with the polyclonal mlL-17 antibodies or serum were given intraperitoneally at two hours before enema with TNBS,respectively,and the mice were killed at 48 hours after enema with TNBS.The histological score of colon and myeloperoxidase (MPO) activity of colonic tissue were evaluated in each group.IL-23p19 and IL-17 concentrations in colonic tissue were measured by enzyme-linked immunosorbent assay (ELISA).Expression of nuclear factor (NF)-κBp65in colonic tissue was detected by immunohistochemistry method.Expression of IL-23p19,IL-17 and IL-12p35 mRNA in colonic tissue were detected by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR) with SYBR Green I.Results The protein levels of IL-23p19 in colonic tissue in TNBS colitis groups at 24 hours,48 hours and 7 days were (15.53±3.32),(31.16±4.98) and (14.03±3.56) ng/mg,respectively,and their mRNA level were (4.09±0.34),(3.39±0.46) and (6.54±1.82),respectively.The protein levels of IL-17 were (0.35±0.06),(0.38±0.08),and (0.26±0.05) ng/mg,respectively,and their mRNA level were (4.21±2.61),(2.65±0.91) and (5.63±1.43),respectively.The expression levels of IL-23p19 and IL-17 in colitis model were significantly higher than those in control group and the peak was at 48 hours.Moreover,expression of IL-23p19 and IL-17 and their mRNA were positively correlated to their mRNA levels.In TNBS 48 hours + mIL-17 antibodies group,the expression levels of NF-κBp65,the microscopic scores and MPO (1.86 % ± 0.36 %,0.63 ± 0.52,0.40 ± 0.03 U/g,respectively) were significantly lower than those in TNBS 48 hours group (4.35% ±0.37%,5.13±0.64,2.29±0.40 U/g tissue,respectively).Neutralization of IL-17 was significantly protected against TNBS-induced colonic inflammation and MPO and expression of NF-κB p65.The results indicated that neutralization of IL-17 significantly reduced colonic inflammation and suppressed NF-κBp65 activation.This protection occurred in the presence of equivalent induction of local IL-23 p19 and high levels of IL-12p35 in the polyclonal raiL-17 antibodies-treated mice.Conclusions IL-23/IL-17 axis plays a critical role at the early acute phase of TNBS-induced inflammation.IL-17 may represent a new target for therapeutic intervention for inflammatory bowel disease.

Adolescent ; Adult ; Aged ; Agranulocytosis ; complications ; drug therapy ; Anti-Bacterial Agents ; administration & dosage ; therapeutic use ; Child ; Female ; Humans ; Leukemia ; complications ; drug therapy ; Male ; Middle Aged ; Treatment Outcome ; Vancomycin ; administration & dosage ; therapeutic use ; Young Adult

[Objective] To investigate the causes of failures of long bone fractures with internal fixations and to study the treatments and effects of reoperations of long bone fractures with inter-locking intramedullary nail and limited open reduction.[Method]Twenty-four patients of failures of long bone fractures with internal fixations were retreated with inter-locking intramedullary nail and limited open reduction.There were 15 cases of femoral fracture,7 cases of tibial fracture and 2 cases of humerus shaft fracture.Of the femoral fracture,there were 14 cases of fractures of shafts of femurs and 1 case of supracondylar fracture of femur;11 male,4 female;7 left cases,8 right cases;1 case complicated with bending of bone plate and screw,6 cases with breakages of plate;1 case with bending of common intramedullary nail,2 cases with breakages of common intramedullary nails,1 case of screw and wire lock fixation complicated with pseudoarticulation formation,4 cases with nonunion of fracture.Of the cases of tibial shaft fracture,there were 6 male,l female;3 left cases,4 righe cases;3 cases complicated with breakages of bone plate and screw,1 case with failure of external fixator,3 cases with nonunion of fracture.Of the cases of humerus shaft fracture,there were 2 cases of male and left ones;1 case complicated with breakage of bone plate and screw,1 case with breakage of common intramedullary nail.The ages of patients ranged from 18 to 68 years old with mean ages 43.7 years old.All fixation failure cases were retreated with inter-locking intramedullary nails and limited open reductions with autogenous iliac bone grafts or bone allografts.[Result]The average follow-up time was 25 months(12～35 months).All cases were cured with bone unions.Complications with submerge,breakages and bending of intramedullary nails did not occur.In the reoperating cases of femoral and filial fractures,the knee function were excellent in 18 cases,good 2 cases,fair 1 case and poor 1 case according to Karlstrom scale.Among the reoperating cases of humeral fractures,there were 1 case excellent and 1 good of shoulder function according to HAN score.[Conclusion]Reoperations with inter-locking intramedullary nail and limited open reduction are effective methods for treatment of internal fixation failure of long bone fracture.

Objective To evaluate the clinical efficiency of partial splenic embolization on portal hypertension patients of hepatocellulocarcinoma(HCC).Methods 110 patients of hepatocellulocarcinoma with portohypertension were treated with 1-3 times of TACE, and partial splenic embolization, with PVA of 355～500 ?m injecting into the arteries of inferior splenic pole. In order to control the area of splenic embolization, multi-embolizations were done according to the various conditions. Results 172 times of embolization were performed in 110 patients involving 134 times of fever lasting for 3-30 days and 126 times of bellyache with 27 times appealing for morphine. Treatable hydrothorax appeared in six, and no serious complications occurred. The area of embolization covered 30%～60%. WBC and BPC counts were increased after the 24 hour (P

Objective To discuss the clinical value of preoperative embolization of vertebral tumors. Methods Data of 54 patients were retrospectively studied. Thirteen lesions were in the cervical, 17 in the thoracic, 15 in the lumber and 9 in the sacral regions. The vertebral tumors were embolized prior to operation with gelatin sponge particles, polyvinyl alcohol (PVA) particles or coils. Surgical operations were performed within 1-7 days after the embolization. Results Totally 79 vessels of 43 patients were embolized, which included ascending cervical artery,1;vertebral artery,1;intercostal artery,39; lumber artery,19;internal iliac artery,15 and median sacral artery,4. Intraoperative hemorrhage showed a mean value of 1312 ml with range of 300-3000 ml in patients after embolization, and a mean value of 2375 ml with range of 1500-5000ml in patients without embolization at the same period(P