Objective To understand the drug resistance status of extended-spectrum beta-lactamase(ESBLs)-producing Esche-richia coli (E.coli)and Klebsiella pneumonia (KPN ),and the prevalence situation of TEM and SHV genotype in Wusu city.Meth-ods E.coli and KPN isolated in two hospitals of Wusu city from July 2011 to June 2013 were collected and the drug sensitivity test was performed by the disk diffusion method (K-B method),the ESBLs preliminary screening and the phenotypic confirmatory test were carried according to the guideline of CLSI,and at the same time DNA of ESBLs producing strains was extracted and the TEM and SHV genes among them were tested by PCR and the electrophoresis method.Results Totally 221 strains of E.coli and 153 strains of KPN were collected,among them ESBLs producing strains were 37 strains and 43 strains respectively,and the detec-tion rate of ESBLs in KPN was higher than that in E.coli(χ2 =6.942,P <0.01).The drug resistance of two kinds of bacteria were similar,the resistance rate of ESBLs producing strains to beta lactam antibacterial drugs was obviously higher than that in non-en-zyme producing strains,which was mostly more than 90%;the resistance rate to penicillin and the first or second generation cepha-losporins was almost 100%;which to gentamicin and ciprofloxacin was above 50%;while which to carbapenem was lower,less than 5%.The detection rates of TEM and SHV genes were 72.9% and 54% in ESBLs producing E.coli and 81.4% and 65.1% in KPN respectively,the detection rates were basically consistent without statistically significant differences between them (P >0.05).Conclusion The detection rate of ESBLs producing E.coli and KPN in Wusu city is higher,the drug resistance situation is severe,and TEM,SHV are the important genotypes of ESBLs.

The purpose of this study was prospectively to research the relationship between EEG power spectrum and anesthesia depth. Twenty-four patients,aged 20 to 50 years , ASA grade ,scheduled for selected surgical operations, were randomly divided into two groups:enflurane and isoflurane groups. Anesthesia was induced by thiopental 5 mg/kg and atracurium 6-7 mg/kg intravenously and maintained by inhalation anesthetics. After intubation patients were ventilated mechanically to maintain the level of PETCO2 between 4. 27 and 4. 93 kpa. NIBP,HR,SpO2 , ECG and EEG were recorded during anesthesia. According to TOF stimulation test intravenous atracurium 10-15 mg was given intermittently to maintain the ratio of T4/T1 less than 25%. Two channels EEG monitor was applied with the filtration range from 0 to 30 Hz Anesthesia level, adjusted at 0. 5,0. 8, 1. 0, 1. 3, 1. 5, 1. 3, 1. 0, 0.8 or 0. 5 MAC in sequence ,was maintained for 10 to 15 minutes before SEF (spectral edge frequency)and MPF (median power frequency) were recorded. NIBP decreased and HR increased with the inhaled agent concentration (IAC) level rising. The lowest BPs were 13. 1 /9. 33 kPa and 12. 9/8. 1kpa in both groups. There were negative remarkable correlations between either SEF or MPF and IAC in both groups (=- 0. 94 and -0. 82,P

Adult ; Endometriosis ; complications ; metabolism ; Endometrium ; metabolism ; Female ; Humans ; Infertility, Female ; etiology ; metabolism ; Leptin ; metabolism

Air ; analysis ; Hydrobromic Acid ; analysis ; Ion-Selective Electrodes ; Workplace

Background:Helicobacter pylori(Hp)is an important pathogen for peptic ulcer and gastric cancer,and is reportedly associated with a variety of extragastrointestinal diseases. However,there is no body fluid detection technique for Hp infection in clinical practice. Aims:To identify Hp infection-associated differentially expressed proteins in urine with relative molecular mass more than 10 kDa and provide potential biomarkers for diagnosis of Hp infection through body fluid detection. Methods:Midstream urine was collected from volunteers in the morning,and 13 C-urea breath test was performed to determine Hp infection. Each of 15 Hp-negative and 15 Hp-positive urine samples were mixed respectively for protein extraction. Spectra data were acquired by high performance liquid chromatogram-mass spectrometry,and label-free technology was used for relative quantitative analysis. The other 26 urine samples(15 Hp-negative and 11 Hp-positive) were used for validation by full scan. IPA software was employed for bioinformatics analysis. Results:A total of 475 urinary proteins were detected by label-free quantitative analysis and 42 differentially expressed proteins were identified. Finally,11 significantly up-regulated differentially expressed proteins were confirmed by external scanning validation. Bioinformatics analysis revealed the molecular functions,biological pathways,and related diseases of these differentially expressed proteins. Conclusions:These 11 differentially expressed proteins more than 10 kDa identified in urine might be potential biomarkers for diagnosis of Hp infection and provide molecular evidence for the correlation of Hp infection with extragastrointestinal diseases.

Objective To explore the clinical and MR imaging features of myxoid liposarcoma.Methods Clinical and MR imaging data of 7 patients with histologically confirmed myxoid liposarcomas on extremities were retrospectively analyzed.The age of the patients ranged from 41 to 59 years with a median age of 51 years.Results Three tumors occurred in thigh,two in calf,one in foot and one in shoulder.Six tumors were situated deeply,and one was superficial.On T_1-weighted images,all 7 tumors showed predominant isointense or slightly hypointense signals relative to muscle,with 6 having lacy,linear or amorphous loci of high signal intensity.The major portion of each tumor displayed hyperintense signals compared with fat on T_2-weighted images.Following the injection of Gd-DTPA,all tumors showed inhomogenous and strong enhancement.All tumors had septa and were well defined without obvious surrounding edema and invasion of the adjacent bones.Conclusion Myxoid liposarcomas usually show predominant isointense or slightly hypointense signals relative to muscle on T_1-weighted images and hyperintense signals relative to fat on T_2-weighted images.The fat components within the tumors may be identified as linear,lacy or amorphous foci of high signal intensity on T_1-weighted images.The contrast enhancement of the mvxoid liposarcomas is usually pronounced and heterogeneous.

The strategy for relocation of computer center room in hospital is expatiated.A set of measures are adopted such as careful planning,detailed design,aborative preparation,reasonable technique strategies,etc.The corresponding management system is established and.Finally,the computer center room is successfully relocated in one time without interruption of hospital information system.

20 first mandibular premolars were randomly divided into 2 groups(n =10).The teeth in experimental group were treated by fi-ber main post in combination with auxiliary pile,in control group by single fiber main post,and then were restored by metal crown.They were fixed in universal testing machine.The fracture load(N)of experimental and control group was (846.50 ±40.33)and (437.90 ±41.15) respectively(P <0.01).

Objective:To investigate the expression and clinical significance of Nusap1 in hepatical carcinoma. Methods:The expression of Nusap1 protein in 61 specimens of hepatical carcinoma was examined by immunohistochemistry. Based on the levels of Nusap1 expression, the 61 specimens were divided into a high Nusap1 expression group and a low Nusap1 expression group. The correlation between Nusap1 expression with clinicopathologic features and prognosis of hepatical carcinoma was analyzed. Results:TherateofhighNusap1expressionwas54.1%inhepaticalcarcinoma.TherateofhighNusap1 expression was 21.3%in noncarcinoma, with signiifcant difference between the 2 groups (P<0.01).Nusap1 overexpression had signiifcant correlation with histological differentiation, tumor size, liver cirrhosis,lymphaticmetastasis,tumorthrombiandearlyrecurrence(P<0.05),butnotwithsex,age,AFP level,tumornumber,TNMclassificationandtumorencapsulation(P>0.05).Survivalanalysissuggested thatthe6monthand12monthnoncarcinomasurvivalratewassignificantlylowerinthehighNusap1 expression group [33.3%(11/33), 17.9%(5/33)] than that in the low Nusap1 expression group [89.3%(25/28), 53.6%(15/28);P<0.005]. Conclusion:Nusap1 is overexpressed in hepatical carcinoma and is a valuable prognostic factor for hepatical carcinoma.

A turbid solution was formed through the reaction between ?-PGA and cetylpyridinium chloride(CPC) and the turibidity was measured by using ultraviolet-visible spectroscopy at 680nm.The linearity between the concentration of ?-PGA and its absorbance,the stability,repeatability and recovery of the method were studied.Through the reaction of ?-PGA with CPC,a homogeneous turbid solution was formed.The turbid solution was stable in 3h under proper pH value and ion strength,the absorbance of the solution at 680nm had a good linear relationshi Pwith the concentration of ?-PGA in the range 12.5~50?g/ml(R2=0.9939).The recovery was within the range of 86%~99.75%(n=5).The relative standard division of the method for determining ?-PGA at the concentrations of 5,10 and 40?g/ml were 0.14%,0.23%和0.025% repeatability.The turbidmetric method has advantages of convenience,simplicity and good repeatability and can be used to control the quality of ?-PGA and its products.