Cholangitis, Sclerosing ; complications ; Histiocytosis, Langerhans-Cell ; etiology ; Humans

Humans ; Minimally Invasive Surgical Procedures ; Neurosurgery ; methods

The degradation kinetics of chlorogenic acid (5-CQA), cryptochlorogenic acid (4-CQA), and neochlorogenic acid (3-CQA) in aqueous solution at 37 degrees C and different pH values (7.05, 7.96, 9.25) were investigated in the present work. The results indicated that 3-, 4- and 5-CQA tended to remain stable in acidic pH circumstance, and unstable in neutral and alkaline pH circumstance. With the increase of the alkalinity, the degradation of 3-, 4- and 5-CQA was increased leading to a less amount of total CQA and was satisfactorily described by the Weibull equation. Meanwhile, caffeic acid was not detected after the degradation of CQA. Moreover, the degradation of 3-CQA and 5-CQA tended to be converted to 4-CQA, and the degradation of 4-CQA tended to be converted to 3-CQA rather than 5-CQA. The comparison of the degradation kinetics parameters of 3-, 4- and 5-CQA at neutral and alkaline pH values showed that the orders of the rate constant (k) values were 4-CQA > 3-CQA > 5-CQA, while the orders of the degradation half life (t½) values were 4-CQA < 3-CQA < 5-CQA, indicating the orders of the stabilities of 3-, 4- and 5-CQA at 37 degrees C and neutral and alkaline pH values were 4-CQA < 3-CQA < 5-CQA.
Caffeic Acids ; Chlorogenic Acid ; analogs & derivatives ; chemistry ; Hydrogen-Ion Concentration ; Kinetics

Objective: To investigate the risk factors of road traffic injuries caused by electric bicycles, so as to provide insights into the prevention and control of road traffic injuries. Methods: A case-control study was performed (cases to controls ratio of 1∶2). The riders of electric bicycles with road-traffic injuries in Songjiang District, Shanghai Municipality during the period between June 2020 and May 2021 were included in the case group, and age-, gender- and occupation-matched riders of electric bicycles without road-traffic injuries in the same study area during the same study period served as controls. Participants' demographic data, living habits, health status, awareness of safe riding and riding behaviors were collected using questionnaire surveys, and the risk factors of road-traffic injuries caused by electric bicycles were identified using a multivariable conditional logistic regression model. Results: There were 167 participants in the case group, including 122 men (73.05%) and with an age of 38 (21) years, and 334 participants in the control group, including 244 men (73.05%) and with an age of 32 (18) years. Multivariable conditional logistic regression analysis identified sleep duration of less than 8 h (OR=1.760, 95%CI: 1.111-2.786), occasional/frequent anxiety (OR=5.140, 95%CI: 1.067-24.750), unawareness of riding speed limit of 25 km/h (OR=2.352, 95%CI: 1.460-3.788), having been warned or punished for violating traffic rules (OR=2.246, 95%CI: 1.243-4.060), running a red light (OR=1.725, 95%CI: 1.043-2.852), riding speed of ≥ 30 km/h (sometimes, OR=2.409, 95%CI: 1.475-3.933; often, OR=4.711, 95%CI: 2.153-10.309) as risk factors of electric bicycle road-traffic injuries. Conclusions Electric bicycle road-traffic injuries is associated with sleep duration, anxiety, awareness of traffic rules, and unsafe riding behaviors. Traffic safety education and law enforcement is required to be reinforced among riders of electric bicycles.

ObjectiveTo analysis the different types of bacteria in patients with a varying severity of acute cholangitis.Methods241 patients who presented with actue cholangitis between Jan 2008 to Oct 2011 to our hospital were retrospectively studied.These patients were divided into three groups according to the Tokyo Guideline.The bile specimens were obtained intraoperatively.The parameters were compared by SPSS 16.0 package program for statistical analysis.Results75.1 percent of the patients were positive for bacteria in the bile.There were 241 strains of bacterium which included 139 Gram-positive bacteria,99 Gram-negative bacteria and 6 strains of microzyme.Escherichia coli,Enterococcus faecalis,Enterococcus casseliflavus,Pseudomonas aeruginosa and Klebsiella species were the most frequently isolated bacteria.The positive rates for bacteria were significantly different between patients with mild and severe cholangitis (P＜0.05).There was no significant difference between patients with moderate and mild cholangits (P=0.141),or moderate and severe cholangitis (P=0.647).Gram-negative bacteria were more common than Gram- positive bacteria in patients with moderate and severe acute cholangitis (P＜0.05).In patients with moderate and severe acute cholangitis,there was more patients with multiple and mixed bacterial infection.ConclusionsEscherichia coli and Enterococcus species were more common in patients with acute cholangitis.The positive rate of bacteria in the bile in severe acute cholangitis was higher than that in mild acute cholangitis.In patients with moderate and severe cholangitis,Gram-negative bacterial infections and multiple and mixed bacterial infections were more common.

Tanshinones and phenolic acids are two major classes of effective compounds in S.miltiorrhizae Radix et Rhizoma.Accumulative levels of these compounds directly determined its quality and efficacy.Presently,for the purpose of understanding the underlying the mechanism behind the biosynthesis and regulation of effective compounds in S.miltiorrhiza,a series of progresses have been pressed ahead.Benefiting from the boost and convenience of high-throughput genomic,transcriptomic,and metabolic analysis methods,remarkable achievements have been accessed in this field.In this paper,a summary for the recent progress have been made with the provision of a reference for better understanding over the quality of S.miltiorrhiza.

Objective To investigate the effects of epidermal growth factor (EGF) on the proliferation , migration and apoptosis of adipose-derived stem cells (ADSCs) in vitro. Methods Starved cell model and FasL-induced apoptosis model were established in serum-free media. The effects of 10 nmol/L and 100 nmol/L of EGF on the proliferation, migration and apoptosis of ADSCs in vitro were observed. The exrpessions of signal pathway proteins like phospholipaseC-γ(PLC-γ) , extracellular regulated kinase (ERK) and AKT were also detected. Results The proliferation, migration and anti-ap-optosis of ADSCs were promoted by 10 nmol/L or 100 nmol/L of EGF, and the expressions of PLC-'y, ERK and AKT were up-regulated. Conclusion EGF can promote the proliferation, migration and an-ti-apoptosis of adipose-derived ADSCs in vitro.

Adenocarcinoma ; surgery ; Adenoma ; surgery ; Adult ; Aged ; Female ; Follow-Up Studies ; Gastrointestinal Stromal Tumors ; surgery ; Hemangioma ; surgery ; Humans ; Ileal Neoplasms ; surgery ; Jejunal Neoplasms ; surgery ; Laparoscopy ; methods ; Lymphoma ; surgery ; Male ; Middle Aged

Objective To explore the clinical value of false positive of Down’ s Syndrome Screening for premature rupture of membranes in the second trimester of pregnancy. Methods From Jan. 2009 to Jul. 2013, there were 321 cases who were in the second trimester of high risk pregnancy recieved Down’ s Syndrome Screening. Their fetals have been excluded chromosome or organ abnormality, and there was no fetal organ structure abnormal through sequence prenatal ultrasound examinations. Results of their pregnancy have been followed-up, and pregnancy outcomes of 346 cases who were of low risk were followed-up at the same time. Results Among the 321 cases, there were 14 ca-ses of abortion because of PROM ( gestational age less than 28 weeks);17 cases of premature delivery because of PROM, including 9 cases whose gestational age were from 28 to 34 weeks and 8 cases whose gestational age were from 34 weeks to 37 weeks;and 7 cases of neonatal asphyxia. In the low risk group, abortion because of PROM occured in 6 cases before 28 weeks, 3 cases between 28 to 34 weeks, and 4 ca-ses between 34 to 37 weeks, and neonatal asphyxia occured in 3 cases. Conclusion Comparing with Down’ s screening false positive preg-nant women and the low risk group, the false positive group have a higher occurrence of PROM, Down’ s screening is a potential high risk in-dex as a predictor of PROM.

Antigens, CD20 ; metabolism ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Diagnosis, Differential ; Humans ; Leg ; Lymphoma, Follicular ; metabolism ; pathology ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Skin Neoplasms ; drug therapy ; metabolism ; pathology