Objective:To investigate the effect of X-irradiation on Adhesion molecules and their related biological characteristics of Human Lung.Methods:A549 cells were irradiated with varying doses of X-rays.MTT assay was employed to evaluate the effects of X-irradiation on the proliferative inhibition of A549 cells.The immunocytochemical method was employed to analyze the protein expression levels of E-cadherin and ?-catenin.The transwell chambers were employed to evaluate the invasion of A549 cells.Results:X-irradiation could inhibit the proliferation of A549 cells in a dose-dependent manner.The protein expression of E-cadherin and ?-catenin in A549 were higher than that in control group(P

Cerebral white matter damage is the most common form of brain injury in premature infants,which is the main cause of neurological and mental injury and cerebral palsy.Imaging examination plays an important role in the diagnosis and follow-up of white matter damage.Because of the safe and accurate characteristics,nuclear magnetic resonance has become the most common imagine form.Different from the traditional nuclear magnetic resonance technique,diffusion tensor imaging (DTI) can get quantitative analysis of cerebral white matter fiber bundle in vivo.This technology has become a powerful tool for the evaluation of white matter damage.The article summarizes the application of DTI in the neurodevelopmental evaluation of cerebral white matter damage in premature infants.

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OBJECTIVE: To optimize the formulation of ketamine hydrochloride for intranasal administration that was of effective anesthetic effect.METHODS: Using ketamine hydrochloride injection as basic preparation,various nasal absorption enhancer or viscosity modifiers(0.5%,1% and 2% azone,EDTA,carbomer,2-hydroxypropyl-?-cyclodextrin,carboxymethyl cellulose) were added to make different nasal drops.After intranasal administration,the anesthetic effect was evaluated to find the optimum formulation.The behavior of anesthetic induction period and anesthetic index were used to evaluate the anesthetic effect.RESULTS: The perfect anesthetic effect was achieved when the formulation was prepared by adding 1% azone in ketamine hydrochloride injection(at a dose of 10 mg?kg-1).The anesthetic time was long.The muscle relaxing time,eyelid reflecting disappearing time,pain disappearing time and righting reflecting disappearing time were(21.4?7.1) min,(11.4?4.6) min,(15.6?5.7) min and(12.0?4.8) min,respectively.CONCLUSIONS: Optimal formulation of ketamine hydrochloride for intranasal administration was obtained when 1% azone was added as an absorption enhancer.

Objective:To explore the application effect of 3D printing teaching model on spinal surgery teaching.Methods:A total of 50 eight-year clinical medical students of Batch 2015 were selected and randomly divided into two groups. Traditional teaching mode was used in the control group and 3D printing teaching model was used in the experimental group. The performance of graduation from the department, teaching experience indicators and teaching satisfaction were compared between the two groups. SPSS 22.0 was used for t test. Results:The performance of the experimental group was higher than that of the control group ( P < 0.05). The scores of teaching experience indicators in the experimental group were all higher than those in the control group ( P<0.05). The satisfaction rate of the experimental group was higher than that of the control group ( P < 0.05). Conclusion:3D printing teaching model can significantly improve students' theoretical knowledge assessment and surgical design evaluation, and improve the teaching effect and students' satisfaction.

Acrylonitrile ; toxicity ; Animals ; Male ; Mice ; Mice, Inbred Strains ; Testis ; drug effects ; pathology

Objective To explore the risk factors of acute organophosphorus pesticide poisoning(AOPP). Method The patients with acute organophosphorus pesticide poisoning,admitted from September 2004 to August 2005,were retrospectively analyzed.The 87 patients were divided into groups according to the presence or absence of combined conditions including hypotension,hypoxemia or metabolic acidosis.Acute physiology and chronic health evaluationⅡ(APACHEⅡ)score was calculated.The chi-square test was used to examine the mortality between those groups.Results The total in-hospital mortality of 87 acute organophosphorus pesticide poisoning patients was 21.8%.The mean APACHEⅡscore was(7.58?5.32)in the 68 survivors and(21.17～9.46)in the 19 dead,there were significant differences between the survivors and the dead(t=9.25,P20 was 65.2%(15/23),andit was 6.3% (4/64)in patients with APACHEII score

Objective To observe the protective effect of grape seed proanthocyanidin extract ( GSPE) on learning disabilities after radiation injury brain in rats.Methods 120 male Wistar rats were divided into control group,model group,high and low dose GSPE groups.The radiation injury brain models were established using a method of linear accelerator irradiation in 22 Gy.The morphological changes of neu-rons in hippocampus were observed with HE staining; the expression of phosphorylated extracellular signal regulated kinase 1/2 ( ERK1/2) and growth associated protein-43( GAP-43) were detected by immunohisto-chemistry;the contents of malonalde hyde(MDA) and superoxide dismutase(SOD) were measured respec-tively by thiobarbituric acid( TBA) and xanthine oxidation( XTO) methods;the learning ability was assessed with shuttle box.Results Compared with the model group,the GSPE groups showed a decreased degree of nerve cell morphological injury; and increased ERK1/2 activities((13.20±1.45)/view,(27.40±2.52)/view,(19.80±1.30)/view),GAP-43 expression level((52.93±2.07)/view,(66.50±0.77)/view,(73.05± 2.40)/view) and SOD activities((79.82±5.26) U/mg,(76.20±6.86) U/mg,(80.12±5.76) U/mg)(P<0.05);and decreased the contents of MDA((71.62±1.88) μmol/g,(76.41±1.94) μmol/g,(72.32±1.98)μmol/g)(P<0.05).Shuttle box testing showed that the active avoidance reaction rate was increased(7 d:(56.23±2.56)%;14 d:(44.66±2.79)%;28 d:(50.40±2.16)%)(P<0.05)and passive avoidance latency was shorted(7 d:(34.11±1.57)s;14 d:(45.52±1.88)s;28 d:( 39.52±1.79)s)(P<0.05) in GSPE groups. The above mentioned changes were more significant in high dose of GSPE(P<0.05) .Conclusion GSPE has protective effect on learning disabilities of radiation injuries brain,which is related to enhance ERK1/2 activ-ity and the expression of GAP-43.

Objective To investigate the cause of stoma recurrence after anterior resection of rectal cancer and discover the methods of prevention and treatment.Methods A total 91 patients with stoma recurrence after anterior resection of rectal cancer (or Dixon) were analysed retrospectively between 1985 and 1996. Fourty seven patients experienced re radical resection (Miles), 27 cases palliative resection, and 11 cases only exploration. Thirty two cases had been followed up for 5 years and obtained 1,3,5 year survival rate for re radical radical resection (Miles). Diagnosis and treatment of stomal recurrence after Dixon were evaluated. Results One, three and five year survival rate of re radical resection (Miles) was 93%,77%,45% respectively.Conclusion To amplify blindly the adaptation of Dixon is to raise the rate of stoma recurrence. Digital rectal examination and fiberopic colonoscopy (and biopsy) are very essential methods for the diagnosis of stoma recurrence, and we strive to do re radical resection (Miles) for the patients with stoma recurrence after Dixon's operation.