1.Localization of a gene determining the sensitivity to sevoflurane in autosomal chromosomes of Drosophila melanogaster
Chuangang JIN ; Jun XIAO ; Yuke TIAN
Chinese Journal of Anesthesiology 1996;0(08):-
Objective To study a gene determing the sensitivity to sevoflurane in autosomal chromosomes of Drosophila melanogaster. Methods Virgin females of wild type Drosophila melanogaster(H) were crossed with ebony(e) males to breeding F 1 hybrids ,to measure the ED 50 of e,H and F 1 for sevoflurane. Then, e virgin females were crossed with F 1 males to breed F 2 hybrids. Sevoflurane ED 50 of F 2 hybrids was measured according to body's colour. The ED 50 was measured when the fruit flies were 7 days old.A hereditary analysis was conducted to determine the localization of gene(s) in chromosome determining sensitivitiy to sevoflurane. Results The ED 50 of H was significantly higher than that of e . The ED 50 of phenotype of F 1 hybrids was similar to one of H of F 1.The number ratio of flies with black abdomen to ebony flies was about 1∶1 in F 2 hybrids which generated from crosses of F 1 hybrid males with e virgin females. The sevoflurane ED 50 of F 2 hybrids was significantly higher than that of F 2 fly of e and was significantly lower than the ED 50 of F 2 fly of H . The dose response curve of F 2 hybrids had a two peak, however, the dose response curve of F 2 fruit fly of e or H had a single peak. Conclusions The gene(s) or major gene(s) determining the sensitivity to sevoflurane is (are) located on the second chromosome of Drosophila melanogaster.
2.Expression of Matrix Metalloproteinase-1 and Tissue Inhibitor of Metalloproteinase-1 in Fibra Muscular Torticollis in Children
jun-zhang, LI ; ke-xiu, XUE ; tian-xiao, WANG
Journal of Applied Clinical Pediatrics 2006;0(16):-
Objective To explore the expressions of matrix metalloproteinases-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in sternomastoid muscle and explore the pathogenesis of sternomastoid muscle fibrosis in congenital fibra muscular torticollis in children.Methods The hyperplastic state of collagen fiber were determine by Masson collagen stainning method and muscular torticollis and fibra torticollis was differed,obtained 22 cases of the fibra torticollis group.Immunohistochemical method was used to determine the expression of MMP-1 and TIMP-1 in sternomastoid muscle of fibra torticollis and compared them with 6 cases of control group.Results By the immunohistochemical method,the expression of MMP-1 in the experiment group significantly decreased than that in control group(P0.05).Conclusion In congenital fibra torticollis,the sternomastoid muscle fibrosis is related to the decrease of MMP-1.
3.Analysis on risk factors for venous thromboembolism in elderly patients with lung cancer
Jun XIAO ; Jing TIAN ; Fang GAO ; Haiyan ZHOU
Chinese Journal of Geriatrics 2015;34(7):723-725
Objective To investigate the risk factors for venous thromboembolism in elderly patients with lung cancer,in order to provide theoretical basis for clinical prevention and treatment.Methods A retrospective analysis was carried out in 869 elderly patients who were treated in our hospital from Mar.2010 to Mar.2014.And the venous thromboembolism and its related risk factors in elderly lung cancer patients were analyzed.Results 35 cases (4.35%) complicated with venous tbromboembolism.Multivariate logistic regression analysis showed that adenocarcinoma,basic diseases,and D-dipolymer≥300 μg/L belonged to the independent risk factors for the complication of venous thromboembolism in elderly patients with lung cancer (OR=2.839,1.586 and 10.514,respectively,P=0.007,0.022 and 0.000).Conclusions The risk factors for the complication of venous thromboembolism should be monitored in the treatment of elderly patients with lung cancer.Early anticoagulation therapy should be performed to improve clinical effect and reduce the incidence of complications.
5.New biological dermis cover Pelnac? in wound healing of children with severe trauma
Liang TIAN ; Lin QIU ; Yuexian FU ; Xiaofei TIAN ; Yan LIU ; Jun XIAO
Chinese Journal of Tissue Engineering Research 2014;(16):2601-2606
BACKGROUND:In recent years, tissue engineering has made great progress, and skin tissue engineering is especialy noteworthy. Artificial dermis (PELNAC) is relatively used widely, but there is a lack of relevant reports on wound repair in children. OBJECTIVE:To investigate the clinical efficacy of Pelnac? METHODS:In a retrospective study, 22 patients with the wound of severe trauma were treated with Pelnac as skin graft dressings on treatment of the wounds of severe trauma in children. ? graft, negative-pressure wound therapy and split-thickness skin graft as experimental group (Pelnac? group), and another 19 patients treated with granulation formation dressing and split-thickness skin graft as control group. We colected data including the graft livability, the required re-operative times and the epithelization time after the skin graft. During the folow-up, the skin color and texture of survival skin, subcutaneous fulness, scar hyperplasia and the joint function were also evaluated. RESULTS AND CONCLUSION: In the Pelnac ? group, the graft livability was up to 90% within 10-14 days after grafting. The secondary split-thickness skin graft was required in two cases in the Pelnac? group and in eight cases in the control group. There was a significant difference in the graft livability (P < 0.05). The average epithelization time after the skin graft was (13.86±3.09) days in the Pelnac? group, which was significant shorter than the control group, (19.10±4.62) days, after the first time operation (P< 0.05). During the 10 months folow-up, the survival skin color and skin elasticity in the Pelnac? group was significantly better than that in the control group (P< 0.05). Better subcutaneous fulness and milder scar hyperplasy in the injured sites were obtained in the Pelnac? group compared with the control gorup. Five cases had certain joint function limitation in the Pelnac? group, compared to 10 cases in the control group, with a significant difference (P < 0.05). Artificial dermis Pelnac? has a stronger anti-infectious ability and higher graft livability. Pelnac? graft combined with negative-pressure wound therapy, granulation culture and split-thickness skin graft can shorten the epithelization time, improve wound healing and aleviate harm to the joint function after the skin graft.
6.Design of slow-paced radio-frequency thermo-therapeutic system for liver cancer
Hu-Jun ZHANG ; Fu-Zhou TIAN ; Xiao-Ming HUANG ; Guo-Jun NIE ;
Chinese Medical Equipment Journal 2004;0(09):-
Thermo-therapeutic has been proved to be an effective approach for cancer therapy.This paper introduces a kind of new equipment suitable for liver oncology based on this theory,which uses the RF field as the thermal energy and adopts sequential-delivering technology.Its principle and advantages are introduced in detail.
7.Correction of secondary lip whistle deformities and nasal base depression after bilateral cleft lip repair with lip subdermal soft tissue flap.
Xiao-Chen TIAN ; Xiao-Jie LÜ ; Xiao-Chun HU ; Jun-Nan CHEN ; Li-Long CUI ; Ying LIU ; Hui-Na LIU
Chinese Journal of Plastic Surgery 2013;29(3):164-167
OBJECTIVETo explore a new method to correct secondary lip whistle deformities and nasal base depression after bilateral complete cleft lip (BCCL) repair with lip subdermal soft tissue flap.
METHODSBilateral subdermal soft tissue "C" flaps and "lambda" flap were designed to repair secondary deformities of nasal base and reconstruct vermilion tubercle in patients after BCCL repair.
RESULTSGood results were achieved in all the patients with primary healing. No flap necrosis happened. The result was satisfactory.
CONCLUSIONSWith bilateral subdermal soft tissue "C" flaps and " lambda" flap, nasal base depression deformities and lip whistle deformities can be corrected. It is an ideal method for correction of deformities after BCCL repair.
Cleft Lip ; surgery ; Humans ; Lip ; surgery ; Nose ; Nose Deformities, Acquired ; surgery ; Reconstructive Surgical Procedures ; Surgical Flaps ; Treatment Outcome ; Wound Healing
8.Anti-depressant effect and mechanism of supercritical CO2 extract from Compound Chaigui Fang.
Lei CHEN ; Xiao-Fen ZHENG ; Xiao-Xia GAO ; Yu-Zhi ZHOU ; Xiao-Qing GUO ; Jun-Sheng TIAN ; Xue-Mei QIN
China Journal of Chinese Materia Medica 2014;39(14):2744-2750
The tail suspension test (TST), forced swimming test (FST) and chronic unpredictable mild stress (CUMS) model were used to evaluate the anti-depressant effect of supercritical CO2 extract from Compound Chaigui Fang (FFCGF). A nuclear magnetic resonance (NMR)-based metabonomics combined with multivariate statistical analysis was performed to explore the mechanism of FFCGF. Rats were conducted by CUMS procedure for 28 days and drugs were administrated at the same time. The body weight, sucrose preference, crossings and rearings in open-field tests were evaluated and the urine was collected simultaneously. The metabonomic profiles of rats' urine were analyzed by NMR and potential biomarkers were searched by multivariate statistical analysis. The results showed that administration of FFCGF significantly decreasing the immobility time in FST and TST and improving rats' body weight, sucrose preference, crossings and rearings in CUMS, which were indication that the anti-depressant effect of FFCGF was abvious. Significant differences in the metabolic profile of the CUMS treated group and the control group were observed, which were consistent with the results of behavioral tests. Decreased levels of acetic acid, succinic acid, 2-oxidation glutaric acid and citric acid and increased glycine and pyruvic acid in urine were significantly affected by the CUMS procedure and the 6 biomarkers were reversed evidently after administration of FFCGF. These changes were suggestion that the anti-depressant mechanism of FFCGF was associated with energy metabolism, lipid metabolism and amino acid metabolism.
Animals
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Antidepressive Agents
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chemistry
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isolation & purification
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pharmacology
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therapeutic use
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Behavior, Animal
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drug effects
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Body Weight
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Carbon Dioxide
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chemistry
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Depression
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drug therapy
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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pharmacology
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therapeutic use
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Male
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Mice
9.Effect of levetiracetam as an add-on to partial seizure on cognitive function and quality of life
Bo ZHOU ; Dong ZHOU ; Lin-Yu TIAN ; Zhen HONG ; Jun XIAO ;
Chinese Journal of Neurology 2001;0(03):-
Objective To explore the effect of levetiracetam(LEV)on cognitive function and quality of life of patients with partial seizure.Methods There were two phase for this study.Phase one was a randomized,double-blind,placebo-controlled study for 16 weeks.Phase two was an open label period for 24 weeks.Medicated patients underwent test on the cognitive function and Health Related Quality of Life (HRQOL)at the baseline respectively at the end of 16 weeks and at the end of 40 weeks.Results(1) Compared the result at the end of 16 weeks with the baseline,LEV group used less time to do Wisconsin card sorting test(WCST,reduce scores -110.3),delayed logical memory were improved(reduce scores 3.4,P
10.Breast fibroadenoma:comparative study of pathological features with varied MRI findings
Xiao-Hong WANG ; Wei-Jun PENG ; Wen-Tao YANG ; Ya-Jia GU ; Tian-Xi YANG ;
Chinese Journal of Radiology 2001;0(05):-
Objective To identify histopathologic correlates for the various MRI appearances of breast fibroadenomas.Methods Thirty-eight fibroadenomas in 33 patients(aged 24—57 years)examined with gadolonium-enhanced MR imaging were observed for signal intensity on T_2-weighted images,contrast enhancement,shape,and internal septation,and these findings were correlated with histopathologic findings.All cases underwent surgery and were proved by pathology.Results(1)The lesion shape was lobular,or round in 34 of 38 fibroadenomas(89.5%).(2)The signal intensity on T_1-weighted images was less than or equal to that of fibroglandular tissue in all cases.The signal intensity on T_2-weighted images was highly varible:high T_2 signal intensity was associated with more myxomatous stromal(mean myxoid-sclerotic index value of 1.9),higher stromal cellularity(mean stromal cellularity index value of 2.2); Fibroadenomas with low T_2 signal intensity had stromal that was nearly uniformly sclerotic(mean myxoid- sclerotic index values of 2.8)and low stromal cellularity(mean stromal-cellularity index value of 1.2). Significant differences were found between these two groups,x~2=11.267 and x~2=10.415(P0.05).The degree of contrast enhancement was proved to be related to ages of patients.The enhancement was more intensely in younger patients.(5)Internal septations were identified within nine of 33 enhancing fibroadenomas (27.3%)and appeared to correlated with collagenous bands at histopatholigic analysis.Conclusions Fibroadenomas demonstrate marked histopathologic variability.The resultant variability in the MR appearance correlated with the degree of myxomatous or sclerotic and stromal cellularity.Lobulation and internal septation,which appear to reflect intrinsic growth patterns of fibroadenomas,may provide more reliable information for distinction.Familiarity with the diagnostic features would facilitate to make the differential diagnosis correctly.