China ; Hematopoietic Stem Cell Transplantation ; adverse effects ; methods ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; therapy

Hematopoietic Stem Cell Transplantation ; Humans

China ; Humans ; Multiple Myeloma ; therapy

Graft vs Host Disease ; diagnosis ; therapy ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Postoperative Complications ; diagnosis ; therapy ; Transplantation Conditioning

China ; Hematopoietic Stem Cell Transplantation ; Humans

Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; therapy

Objective: To prepare fluorouracil-loaded chitosan nanoparticle (5-Fu-CS-NP) for ophthalmologic usage and evaluate its releasing characteristics in vitro. Methods: 5-Fu-CS-NP was prepared with 5-Fu, polyacrylic acid and chitosan using dispersion method. The mean size, entrapment efficiency (EE%), drug loading (DL%) and the in vitro releasing characteristics of 5-Fu-CS-NP were investigated. Results and conclusion: The mean size of 5-Fu-CS-NP was (144.6±3.1) nm, the EE% was 84.5% and the DL% was 3.91%. 5-Fu-CS-NP had a satisfactory sustained-releasing effect and the releasing could last for 3-7 days, and the release behavior was not greatly changed within a pH value of 7.2-7.4.

Objective: To investigate the effects of resveratrol(RES)on the bone mineral density (BMD) in ovariectomized female rats. Method: Forty-eight SD female rats were randomly divided into 6 groups and treated respectively as follows:group A, sham operated; group B, ovariectomized (OVX); group C, OVX supplemented with 0.03 mg/(kg bw ?d)diethylstilbestrol; and group D, E , F: OVX rats supplemented with RES at 5, 15 and 45 mg(/kg bw ?d). The duration of exposure was 90 d and the BMDs of rats were measured by dual-energy X-ray absorptiometry (QDR-4500A). Results: BMDs at all measured sites of group B were significantly lower than those of group A. And compared with group B, BMDs of the total body, lumbar vertebrae and femur of group D, E, F were increased significantly by RES. Conclusion: The bone loss of the ovariectomized female rats could be inhibited by RES. It seemed that the inhibitory effects of 45 mg/(kg bw ?d)RES on bone loss of ovariectomized female rats were better than the other 2 dosages or 0.03 mg/(kg bw ?d)diethylstilbestrol in this experiment.

OBJECTIVE:To establish the quality standard for Gardenia jasminoides. METHODS:The moisture,total ash and extract were determined. HPLC was used for contents determination of geniposide,rutin,crocinⅠand crocinⅡ:the column was Waters Xbridge-C18 with mobile phase of acetonitrile-0.2% phosphoric acid(gradient elution,gardenia and rutin),methanol-water (45∶55,V/V,crocinⅠ,crocinⅡ)at a flow rate of 1.0 ml/min;detection wavelength was 256 nm for gardenia and rutin,440 nm for crocinⅠ,crocinⅡ;column temperature was 30℃;injection volume was 10μl for gardenia and rutin,5μl for crocinⅠ,cro-cinⅡ. RESULTS:The moisture total ash and ethanol-soluble extract of G. jasminoides were 5.8%-8.4%,3.7%-5.9% and 29.5%-37.9%,respectively. The linear range was 162.08-1 620.84 μg/ml for geniposide(r=0.999 9),2.07-20.72 μg/ml for rutin (r=0.999 9),8.04-80.41 μg/ml for crocinⅠ(r=0.999 9)and 1.05-10.53 μg/ml for crocinⅡ(r=0.999 9);RSDs of precision,sta-bility and reproducibility test were lower than 2%;recoveries were 99.33%-101.43%(RSD=1.09%,n=6),97.97%-101.83%(RSD=1.39%,n=6),97.97%-101.30%(RSD=1.36%,n=6) and 98.65%-103.04%(RSD=1.84%,n=6). CONCLUSIONS:The established standard can be used for the quality control of G. jasminoides commercially available.

Objective:To prepare fluorouracil-loaded chitosan nanoparticle (5-Fu-CS-NP) for ophthalmologic usage and evaluate its releasing characteristics in vitro. Methods: 5-Fu-CS-NP was prepared with 5-Fu, polyacrylic acid and chitosan using dispersion method. The mean size, entrapment efficiency (EE%), drug loading (DL%) and the in vitro releasing characteristics of 5-Fu-CS-NP were investigated.Results and conclusion: The mean size of 5-Fu-CS-NP was (144.6?3.1) nm, the EE% was 84.5% and the DL% was 3.91%. 5-Fu-CS-NP had a satisfactory sustained-releasing effect and the releasing could last for 3-7 days, and the release behavior was not greatly changed within a pH value of 7.2-7.4.