S100A8 and S100A9 are abundantly expressed in neutrophils cytoplasm,they are calciumbinding proteins and they often exist as S100A8/A9 heterodimer.Previous studies have shown that the biological functions of S100A8 and S100A9 are associated with chronic inflammatory diseases and a variety of cancers.They are important to inflammation by binding and activation Toll-like receptor4 (TLR4) and receptor for advanced glycation end products(RAGE),and mediating intracellular inflammatory signaling transduction.This review summarizes the studies on functions and molecular mechanism of S100A8 and S100A9 in autoimmune diseases,which might propose new strategies for diagnosis,treatment and suggested disease activity.

Objective To evaluate the feasible of dual-energy coronary artery CTA combined with affirmed iterative reconstruction technique for overweight patients.Methods 60 cases underwent dual-energy coronary artery CTA were enrolled in this reasearch.Patients were divided into two groups according to different reconstruction arithmetic model:group A with FBP reconstruction model,while group B with SAFIRE reconstruction model and monoenergetic images from 65 to 90 keV (with increment of 5 keV).All the data were measured by one doctor worked in CT department for more than 5 years,and the image quality were analyzed and evaluated by two senior radiologists.Randomized block ANOVA analysis was used to compare objective evaluation indices of different monoenergetic images from 65 to 90 keV to get the best keV level.The comparative t-test was applied to objective evaluation indices(CT values,SD,SNR,CNR and image quality score) of group A and B and comparative x2-test was applied to image quality score at the best keV level.Results There were significant differences in CT value,SD,SNR,CNR of different keV images (P ＜0.05).For objective evaluation of image quality,the SNR and CNR of group B were significantly higher than group A,and SD was significantly lower than group A (P＜0.05).For subjective evaluation of image quality,there were statistically significant difference between group B and group A (P＜0.05),the segment score of group B was superior to group A.Radiation dose:the DLP value was (238.7±30.82) mGy · cm,and the ED value was (3.3±0.43) mSv.Conclusion SAFIRE combine with purified single spectrum technique can improve the image quality and reduce radiation dose in dual-energy coronary artery CTA,and 75 keV is the best single energy level.

Arthritis, Juvenile ; diagnosis ; Child ; Forecasting ; Humans ; Immunologic Tests ; Lupus Erythematosus, Systemic ; diagnosis ; Mucocutaneous Lymph Node Syndrome ; diagnosis ; Rheumatic Diseases ; diagnosis ; immunology ; therapy ; Rheumatic Fever ; diagnosis ; Scleroderma, Systemic ; diagnosis

The organic acidemia/aciduria is one of the most common inherited metabolic disorders in clinic,more than 50 species have been found until now.The illness is believed to be caused by gene mutation,leading to the reduction or loss of enzyme activity and the accumulation of carboxylic acid and its metabolites.The manifestations of increased blood organic acids include refractory metabolic acidosis,paroxysmal vomiting,feeding difficulties,hypotonia,convulsions and disturbance of consciousness.Most of the organic acidemia begins in neonatal period or infancy,accompanied by progressive neurological damages at most of the time.There are little specific clinical features can be found in this kind of diseases,therefore,early diagnosis and treatment must be initiated in order to decrease risk of neurological induries and damages or acute deaths.So application of the gas chromatography-mass spectrometry and tandem mass spectrometry is important to the early diagnosis,helpful for improving the outcomes and reducing child mortality.

Urea cycle disorder is a common inherited metabolic disorder,and it is the most common genetic cause of hyperammoniema in children.The illness is believed to be caused by gene mutation of six main enzymes in urea cycle,leading to ammonia,which is produced by amino acid catabolism,can't conver to urea through the urea cycle and be discharged through the urine.The manifestations of hyperammonemia turn out to be disorders of brain function (refusing to milk,vomiting,drowsiness,coma,convulsions,ataxia,aggressive behaviors).The incidence of this disease is 1/30 000.At the same time,the severity of the clinical symptoms is connected with the extent of the enzymes defects.More serious the enzymes defected,the earlier and worse the disease onsets.Some children with mild enzyme defects may intermittently attack or have a delay onset.Serious nervous system injuries can be found in hyperammonernia,therefore,early diagnosis and treatment must be ensured in order to decrease risk of mental injuries and damages or acute deaths.

Humans ; Macrophage Activation Syndrome ; diagnosis

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BACKGROUND: Gallie, Brooks steel wire, and Halifax vertebral plate clamp are tools for internal fixation of atlas-axis joint. They can keep the flexion-extension stability of atlas-axis joint, but weaken the ability to against lateral bending and rotation. Magerl+Gallie implantation shows good biomechanics stability, but cannot well prevent axial dislocation. OBJECTIVE: To introduce the surgical technique from posterior approach for atlas, and explore the effect on biocompatibility and biomechanics of implants. RETRIEVAL STRATEGY: The articles related to atlas-axis joint dated between January 1970 and June 2007 were retrieved through Pubmed, PML, OVID and Wanfang database using of "atlas, axis, dislocation, fixation, posterior" and "atlas, dislocation, internal fixation, implant, posterior approach, biocompatibility, biomechanics" in Chinese. All collected articles were selected firstly and the articles related to implants, surgical skills, and biomechanics were selected. For articles in identical field, only those published in recently or in authoritative journals were selected. Repetitive articles and Meta analysis were excluded. 127 articles met the criteria and 30 of them were involved. LITERATURE EVALUATION: The articles involved were related to the surgical treatment of dislocation of atlas-axis joint. Of 30 articles, 3 were review articles, 3 were monographs, and the others were clinical or basic researches. DATA SYNTHESIS: ①Lateral mass and pedicle screw implantation techniques can achieve three-dimensional fixation. ②The screw entrance points of atlantoaxial pedicle screw implantation is higher than lateral mass technique, it is unnecessary to expose C1, posterior arch and other deep parts of anatomic structures. C2 nerve root and venous plexus are not separated completely, which reduce injury rate and blood loss. In addition, screw passage of pedicle screw is longer than Harms technique, so screw can completely contact with skeleton to enhance the fixation. CONCLUSION: Atlantoaxial fixation by posterior pedicle screw implantation shows good biomechanics and biocompatibility. It may become the optimal approach for atlantoaxial short segmental fixation.

Objective To compare the therapeutic efficacy and adverse effects between transcather arte-rial chemoembolization(TACE) and comprehensive therapy in primary advanced heptocellular carcinoma(HCC).Methods 216 patients with primary HCC were arranged randomly into two groups:104 (group A) were treated with TACE alone,the rest(group B)were treated with TACE combined with intraperitoneal perfusion chemothera-py, Chinese herbs and immunotherapy.The therapeutic efficacy and adverse effects were analysed and compared between two groups.Results The CR + PR rates were 28.84% in group A and 34.82% in group B.The 4-year survival rates were 1.92% in group A and 3.87% in group B(P < 0.05).There were no severe side effects in both groups.Conclusions The short-term effective rate and 4-year survival rate in comprehensive treatment group were superior to the control group.Therefore, the comprehensive treatment is worthwhile to use in primary advanced hepatocellular carcinoma.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.