S100A8 and S100A9 are abundantly expressed in neutrophils cytoplasm,they are calciumbinding proteins and they often exist as S100A8/A9 heterodimer.Previous studies have shown that the biological functions of S100A8 and S100A9 are associated with chronic inflammatory diseases and a variety of cancers.They are important to inflammation by binding and activation Toll-like receptor4 (TLR4) and receptor for advanced glycation end products(RAGE),and mediating intracellular inflammatory signaling transduction.This review summarizes the studies on functions and molecular mechanism of S100A8 and S100A9 in autoimmune diseases,which might propose new strategies for diagnosis,treatment and suggested disease activity.

Objective To evaluate the feasible of dual-energy coronary artery CTA combined with affirmed iterative reconstruction technique for overweight patients.Methods 60 cases underwent dual-energy coronary artery CTA were enrolled in this reasearch.Patients were divided into two groups according to different reconstruction arithmetic model:group A with FBP reconstruction model,while group B with SAFIRE reconstruction model and monoenergetic images from 65 to 90 keV (with increment of 5 keV).All the data were measured by one doctor worked in CT department for more than 5 years,and the image quality were analyzed and evaluated by two senior radiologists.Randomized block ANOVA analysis was used to compare objective evaluation indices of different monoenergetic images from 65 to 90 keV to get the best keV level.The comparative t-test was applied to objective evaluation indices(CT values,SD,SNR,CNR and image quality score) of group A and B and comparative x2-test was applied to image quality score at the best keV level.Results There were significant differences in CT value,SD,SNR,CNR of different keV images (P ＜0.05).For objective evaluation of image quality,the SNR and CNR of group B were significantly higher than group A,and SD was significantly lower than group A (P＜0.05).For subjective evaluation of image quality,there were statistically significant difference between group B and group A (P＜0.05),the segment score of group B was superior to group A.Radiation dose:the DLP value was (238.7±30.82) mGy · cm,and the ED value was (3.3±0.43) mSv.Conclusion SAFIRE combine with purified single spectrum technique can improve the image quality and reduce radiation dose in dual-energy coronary artery CTA,and 75 keV is the best single energy level.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

Arthritis, Juvenile ; diagnosis ; Child ; Forecasting ; Humans ; Immunologic Tests ; Lupus Erythematosus, Systemic ; diagnosis ; Mucocutaneous Lymph Node Syndrome ; diagnosis ; Rheumatic Diseases ; diagnosis ; immunology ; therapy ; Rheumatic Fever ; diagnosis ; Scleroderma, Systemic ; diagnosis

Humans ; Macrophage Activation Syndrome ; diagnosis

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Objective Liver is a common site of metastasis of gastric cancer and hitherto there has been no uniform treatment for liver metastasis of gastric cancer. This study was to investigate the clinical characteristics and prognostic factors of liver metastasis of gastric cancer in order to provide some evidence for the improvement of the diagnosis and treatment of the disease. Methods We ret-rospectively analyzed the clinical characteristics and survival data of 143 patients with liver metastasis of gastric cancer treated in our hospital between March 2007 and September 2012. We investigated the independent risk factors affecting the prognosis of the disease u-sing the Cox Regression Model. Results Seventy-five ( 52. 4%) of the 143 patients survived for 12 months, 41 ( 28. 7%) for 24 months, and 18 (12.6%) for 36 months, with a median survival time of 14 months. Univariate analysis revealed statistically significant differences in the survival time among the patients with different histological grades of primary gastric cancer, time of liver metastasis, types of metastatic liver cancer, extra-liver metastasis, and treatment methods ( P<0.05) . Multivariate Cox regression analysis showed the histological grade of gastric cancer to be low-or non-differentiation ( HR=2.67, 95% CI:1.51-4.72) and the type of liver meta-static cancer to be H2 ( HR=1.89, 95% CI:1.08-3.29) or H3 ( HR=2.13, 95% CI:1.32-3.44) , which were independent risk fac-tors affecting the prognosis of liver metastasis of gastric cancer, while palliative chemotherapy ( HR=0.55, 95% CI:0.32-0.95) was an independent protective factor of its prognosis. Conclusion Patients with liver metastasis from gastric cancer usually have a poor prognosis, especially those with histologically low-grade gastric cancer or type-H2 or -H3 liver metastatic cancer. The prognosis of thepatients undergoing palliative chemotherapy may have a better prognosis than those receiving best supportive care. A comprehensive therapy should be recommended to patients with liver metastasis from gastric cancer.

Objective: To construct a recombinant lentivirus carrying C57BL/6 mouse estrogen receptor α (Erα) and to infect mouse neurons, so as to pave a way for further studying the relationship of Erα with some nervous system diseases. Methods: Erα gene was inserted into the main virus vector LV-GFP-flag to construct recombinant lentiviral vector LV-Erα-flag, which was confirmed by agarose gel electrophoresis (AGE) and DNA sequencing. Recombinant lentivirus V-Erα-flag was produced by 293T cells following the co-transfection of LV-Erα-flag with the packaging plasmids pHelper 1.0 and pHelper 2.0, and the virus titer was examined. The neurons of C57BL/6 mouse were cultured using a serum-free culture medium, and then control lentivirus V-GFP-flag was used to infect the neurons. The infection efficiency and apoptosis rate were examined by flow cytometry to obtain optimal multiplicity of infection (MOI). GFP expression was detected daily under an inverted fluorescent microscope. After that, V-Erα-flag with the optimal MOI was used to infect neurons; the expression of Erα mRNA and protein in the neurons was detected by quantitative real-time PCR and Western blotting analysis. Results: AGE and DNA sequencing confirmed that LV-Erα-flag was successfully constructed, and packaged into 293T cells with a titer of 2×108 TU/ml. Control lentivirus V-GFP-flag could infect neurons, with the infection efficiency being (89.8±4.03)% and the cell apoptosis rate being only (3.6±0.29)% when MOI = 5. Neurons could survive in the culture for at least 8 weeks, during which the GFP was persistently expressed, indicating the lentivirus could efficiently and stably infect the neurons. The expression of Erα mRNA and protein was greatly increased in neurons infected with V-Erα-flag (MOI = 5). Conclusion: The recombinant lentivirus carrying Erα has been constructed successfully, which can infect neurons and lead to increase the expression of Erα mRNA and protein.

Objective: To explore the anti-inflammatory mechanisms of selective estrogen receptor modulator (SERM) on experimental autoimmune encephalomyelitis(EAE). Methods: EAE models were induced with MOG35-55 peptide in 60 mice and all animals were ovarectomized. The model animals were then divided into treatment group and control group (n = 30). Treatment group was treated with SERM. The clinical symptom scores were compared between the two groups. The pathologic changes of the brain and spinal cord were studied by H-E staining and luxol fast blue(LFB)-HE staining. The expressions of MMP-9, TNF-α, IFN-γ, and IL-4 mRNA and protein were examined by quantitative real-time PCR and ELISA. Western blotting analysis was used to analyze the expression of myelin basic protein (MBP) so as to analyze the demyelination status. Results: Clinical symptom scores and incidence of EAE in the treatment group were improved compared with those in the control group (P<0.01, P<0.05). H-E staining showed that infiltration of inflammatory cells was decreased in the treatment group (P<0.05). LFB-H-E staining and Western blotting analysis showed that the demyelination was improved in the treatment group (P<0.05). The results of quantitative real-time PCR and ELISA showed that the expression of MMP-9,TNF-α, and IFN-γ were decreased and the expression of IL-4 was increased (P<0.05, P<0. 01). Conclusion: SERM can alleviate the inflammation symptom in EAE mice through decreasing MMP-9, TNF-α, and IFN-γ and increasing IL-4 expression.

Clinical risk index for babies( CRIB) is applied in low birth weight preterm neonates to assess the initial severity of illness,predict mortality risk rates,evaluate their own performance,and audit the performance between different medical institutions.CRIB plays an important role in the progressive develop-ment of neonatology.The scoring rules and advantagse of CRIB scorni g system were introud ced in this arti-cle.Teh scoring system is able to correctly predict mortaliyt probabilities and long-term neurodevelopmental outcomes for low birth weight preterm infants.