Objective:To prepare and evaluate the effectiveness of chitosan-carboxymethylcellulose membrane in preventing postoperative intestinal adhesion.Methods: Chitosan-carboxymethylcellulose membrane was prepared with 11 ratio of chitosan and carboxymethylcellulose.Glutaraldehyde and ammonium aluminium sulfate were used for cross-linkage,glycerin for enhancing plasticity;and then the product was dried.The membrane was observed with scanning electron microscopy(SEM) and its tensile strength and breaking elongation were measured.Forty-eight SD rats with ileum injury were randomly divided into A,B and C groups(n=16).During operation,the injury in group A was treated with chitosan-carboxymethylcellulose membrane,in group B with chitosan membrane,and in group C without treatment(control group).The adhesion was observed on the 14~(th) postoperative day.Results: The tensile strength of chitosan-carboxymethylcellulose membrane was 20 MPa and the breaking elongation was 65%.SEM showed that the morphology of the membrane had crossed fibroid structures and irregular pores. The severity of adhesion in group A and B was significantly lower than that in group C(P

Objective:To investigate the effect of carboxymethylchitosan on autocrine growth factor and morphology of fibroblasts cultured in vitro,so as to discuss the possible mechanism by which carboxymethylchitosan alleviates overhealing and prevents adhesion in wound healing.Methods: Fibroblasts were cultured in vitro.Fibroblasts of passage 4-6 were treated with different concentrations of carboxymethylchitosan(0.01,0.1,1.0 and 10 mg/ml) for 4 days or with 0.1 mg/ml carboxymethylchitosan for 1,2,3, 4,5,and 6 days.The levels of autocrine transforming growth factor-?_(1 )(TGF-?_(1)) and epidermal growth factor(EGF) of fibroblasts were determined by ELISA and radioimmunoassay.The fibroblastic morphology was detected by transmission electron microscopy(TEM) and microscope after fibroblasts were treated with different strategies.Results: Carboxymethylchitosan(≥0.1 mg/ml)inhibited autocrine TGF-?_(1) of fibroblast in a time-and concentration dependent manner(P0.05).Carboxymethylchitosan ((≥0.1 mg/ml))also inhibited the proliferation of fibroblasts and caused their ultrastructural changes.Conclusion: Carboxymethylchitosan (≥0.1 mg/ml)can inhibit fibroblasts proliferation and reduce tissue adhesion, possibly through altering fibroblast ultrastructure and selectively inhibiting secretion of TGF-?_(1).

Torque teno virus(TTV)is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3808 nucleotides of the TTV(SANBAN isolate)genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerise chain assembly reaction(PCA),and the synthesis was completed with splicing by overlap extension(SOEing).This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.

Objective To investigate the curative effect of combined traditional Chinese and Western medicine treatment of de-pression after stroke.Methods Patients with depression in 134 cases of cerebral stroke from July 2011 to August 2012 in Tongli-ang People′s hospital were treated in department of internal medicine,and according to the different treatment methods were divided into experimental group and control group,each group of 67 cases.The control group was given paroxetine hydrochloride orally,the experimental group was based on the use of miananning granule in the treatment,obered curative effect of two groups.Results In the experimental group,the control group total effective rate were 95.5%,77.6%.The experimental group total effective rate was significantly better than the control group and has a statistically significance(P<0.05).Conclusion The combination of traditional Chinese and Western medicine treatment of depression after stroke is exact,it is worthy of popularization and application.

Objective:To explore the perioperative safety management of patients with severe limb dysplasia, to select appropriate methods to monitor vital signs, and to prevent intraoperative acquired pressure sore, nosocomial infection and other related complications.Methods:A case of severe developmental disorder of extremities caused by acroscleroderma in November 2019 in Peking University Shenzhen Hospital needed to be amputated. By organizing multidisciplinary consultation to discuss the operation plan, repeatedly selecting appropriate tools, and multiple scenario simulation exercises, continuous transcutaneous oxygen saturation and noninvasive blood pressure monitoring could be realized for the patients with acromegaly deficiency of the extremities. Under the monitoring of color ultrasound, the arterial blocking pressure of the extremities was determined as the tourniquet pressure during the operation value, take appropriate measures to prevent intraoperative acquired pressure sore, nosocomial infection and other related complications.Results:With the cooperation of multidisciplinary team, amputation was successfully carried out for the patients. The position was comfortable during the operation, the operation process was smooth, the residual limbs healed well after the operation, and there was no intraoperative acquired pressure sore, nosocomial infection and other related complications.Conclusions:To establish a multidisciplinary cooperation mechanism, strengthen the construction of the operating room special group, and use evidence-based nursing methods and actual scenario simulation exercise can ensure the perioperative safety of patients with severe developmental disorder of extremities and similar patients with special body shape or very low weight.

BACKGROUND: Electrical muscle stimulation can certainly improve muscle protein synthesis and muscle mass growth, but there are also different research results. OBJECTIVE: To analyze the influence of electrical muscle stimulation as a strength training method on the muscle strength of exercisers through meta-analysis, providing advanced method and evidence-based basis for future strength training in sports field. METHODS: Databases such as PubMed, EBSCO host, Web of Science, CNKI, WanFang, and VIP were searched for randomized controlled trials regarding electrical muscle stimulation for improving the muscle strength of exercisers. The literatures were screened by PICOS principles. The subjects were athletes and healthy controls with exercise experience, and they were divided into electrical stimulation group and control group (without any intervention). According to Cochrane criteria we conduct meta-analysis, sensitivity analysis, subgroup analysis, and publication bias evaluation through Stata 15.1 and Review Manager 5.3. RESULTS AND CONCLUSION: Compared with the control group, electrical muscle stimulation significantly increased concentric contraction peak torque in exercise population (combined effect: weighted mean difference=8.23, 95% confidence interval: 6.71-9.76, P < 0.000 1), and the effect was most obvious at the age of 20-22 years. Compared with the control group, electrical muscle stimulation significantly increased eccentric contraction peak torque in exercise population (combined effect: weighted mean difference=5.58, 95% confidence interval: 4.16-7.00, P < 0.000 01). The number of included experiments about isometric contraction peak torque was small and it was not suitable for meta-analysis. To conclude, electrical muscle stimulation training can effectively promote the concentric contraction peak torque and eccentric contraction peak torque of the exercise population, and the effect of electrical muscle stimulation as a training method on the muscle strength of exercisers needs to be explored in terms of indicators, ages and subjects.

The endophytic fungi with plant growth promoting effects were screened by co-culture of each endophytic fungus and seedlings of Dendrobium officinale. Anatomical features of the inoculated roots were studied by paraffin sectioning. Morphological characteristics and rDNA ITS1-5. 8S-ITS2 sequences were applied for the taxonomy of endophytic fungi. The results showed that 8 strains inoculated to D. officinale seedlings greatly enhanced plant height, stem diameter, new roots number and biomass. According to the anatomical features of the inoculated roots, each fungus could infect the velamina of seedlings. The hyphae or pelotons were existed in the exodermis passage cells and cortex cells. The effective fungi could not infect the endodermis and vascular bundle sheath, but which was exception for other fungi with harmful to seedlings. Combined with classic morphologic classification, 2 effective strains were identified which were subjected to Pestalotiopsis and Eurotium. Six species of fungi without conidiophore belonged to Pyrenochaeta, Coprinellus, Pholiota, Alternaria, Helotiales, which were identified by sequencing the PCR-amplified rDNA ITS1-5. 8S-ITS2 regions. The co-culture technology of effective endophytic fungi and plant can apply to cultivate the seedlings of D. officinale. It is feasible to shorten growth cycle of D. officinale and increase the resource of Chinese herbs.
Biomass ; DNA, Fungal ; genetics ; DNA, Ribosomal ; genetics ; DNA, Ribosomal Spacer ; genetics ; Dendrobium ; cytology ; growth & development ; microbiology ; Endophytes ; classification ; genetics ; physiology ; Fungi ; classification ; genetics ; physiology ; Host-Pathogen Interactions ; Hyphae ; physiology ; Plant Roots ; cytology ; growth & development ; microbiology ; Plants, Medicinal ; growth & development ; microbiology ; RNA, Ribosomal, 5.8S ; genetics ; Seedlings ; cytology ; growth & development ; microbiology ; Species Specificity

Objective To assess the efficacy and safety of leflunomide in active ankylosing spondylitis.Methods Totally 62 cases of active ankylosing spondylitis were included,and divided randomly into leflunomide group(n=26)and methotrexate group(n=36).The patients in leflunomide group received an oral leflunomide loading dose of 50 mg or 40 mg once daily for the first 3 days followed by a maintenance dose of 50 mg or 40 mg once weekly,according to whether the bodyweight was more than 50 kg or less than 50 kg,respectively.The patients in methotrexate group were prescribed oral methotrexate 5 mg once in the first week,10 mg once in the second week,15 mg once in the third week and thereafter weekly.Sulfasalazine was prescribed 0.75 three times daily in both groups.The Bath Ankylosing Spondylitis Disease Activity Index(BASDAI)and The Bath Ankylosing Spondylitis Metrology Index(BASIM)scoring instruments were used to assess the responsiveness to leflunomide and methotrexate at entry,three months,six months,twelve months and eighteen months after treatment,respectively.Laboratory data including peripheral blood counts(WBC,HGB,RBC),erythrocyte sedimentation rates(ESR),liver enzymes,serum creatinine,urinary sediments were detected at the same time.Sacroiliac joints CT scan and other involved joints x-ray radiograph examinations were taken at entry,six months,twelve months and eighteen months after treatment,respectively.Results Twenty-two cases in leflunomide group and 29 cases in methotrexate group were followed up to eighteen months.BASDAI in leflunomide group was significantly lower(P

Objective To study the immunological changes and clinical features of pachydermodactyly.Methods Thirteen cases of pachydermodactyly were studied and the literature about this disease was reviewed.Results The mean disease onset age was (16?3)years,11 of whom were male,2 were female;clinical feature was chronic non-inflammatory bulbous swelling of proximal interphalangeal joints,most of which distributed symmetrically;there were no abnormal results in immunological examination;X-ray showed only soft tissue swelling with no articular or bone damage.Conclusion Pachydermodactyly belongs to rheumatological diseases,and should be differentiated from other arthritis involving PIP joints.

Objective To concentrate coagulation Factor Ⅺ(FⅪ)from human plasma.Methods FⅪwas isolated and purified from human plasma via two-step chromatography,including CM-Sepharose fast flow ion exchange and Heparin CL-6B affinity chromatography.Results The recovery rate of FⅪ was(21.02?5.04)%,the specific clotting activity of purified FⅪ was(17.59?1.96) U/mg,and the purification factor was(1162.29?129.64) fold(n=7).Conclusion The two-step chromatography is effective in concentrating FⅨ.