1.Research Value of Multi-clone Cell Line: A Comment for the 30th Anniversary of J6-1 Human Leukemic Cell Line.
Ke-Fu WU ; Guo-Guang ZHENG ; Xiao-Ton MA
Journal of Experimental Hematology 2007;15(5):909-912
J6-1 cell line is the first leukemic cell line established in China. It is a multi-clone cell line infected with both EBV and HHV-6. Many cytokines, receptors and other genes were cloned from J6-1 cell line since its establishment 30 years ago. Valuable information on leukemic characteristics and functions were obtained from the studies on this cell line, which could be categorized into several research subjects. These achievements implied the unique research value of multi-clone cell lines. This comment focuses attention on research advance of the J6-1 leukemic cell line in 30 years, including heterogeneity and multi-cloning of J6-1 cells, survival mechanism of J6-1 cell populations, abnormal intercellalar communication of J6-1 cells with its significance and inspiration from J6-1 cell line.
Cell Line, Tumor
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Cell Transformation, Neoplastic
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Clone Cells
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Herpesvirus 4, Human
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immunology
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Herpesvirus 6, Human
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immunology
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Humans
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Leukemia
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pathology
2.The quantitative changes of serum response factor in myocardium of patients with heart failure.
Xiao-Xia WU ; Guang ZHI ; Xiu-Zhen LIU
Chinese Journal of Applied Physiology 2003;19(3):289-290
Adolescent
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Adult
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Child
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Female
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Heart Failure
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blood
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Humans
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Male
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Middle Aged
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Myocardium
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metabolism
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Serum Response Factor
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metabolism
3.Analysis on medication rules of state medical master Yan Zhenghua from prescriptions with citri reticulatae pericarpium based on data mining.
Jia-Rui WU ; Wei-Xian GUO ; Bing ZHANG ; Xiao-Meng ZHANG ; Bing YANG ; Xiao-Guang SHENG
China Journal of Chinese Materia Medica 2014;39(4):618-622
The prescriptions containing pericarpium citri reticulatae that built by Professor. Yan were collected to build a database based on traditional Chinese medicine (TCM) inheritance assist system. After analyzed by data mining, such as apriori algorithm, the frequency of single medicine, the frequency of drug combination, the association rules between drugs and core drug combinations can be get from the database. Through the analysis of 1 027 prescriptions with pericarpium citri reticulatae, these prescriptions were commonly used to treat stomach aches, cough and other syndromes. The most frequency drug combinations were "Citri Reticulatae Pericarpium-Poria", "Paeoniae Radix Rubra-Citri Reticulatae Pericarpium" and so on. The drug association rules that the confidence was 1 were "Glycyrrhizae Radix ex Rhizoma --> Citri Reticulatae Pericarpium", "Paeoniae Alba Radix-Cyperi Rhizoma --> Citri Reticulatae Pericarpium", "Poria --> Citri Reticulatae Pericarpium", and so on. The drugs in the prescriptions containing pericarpium citri reticulatae that built by Professor Yan mostly had the effects of regulating the flow of Qi and invigorate blood circulation, which reflected the clearly thought when making prescriptions.
Citrus
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chemistry
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Data Mining
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Drug Interactions
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Drug Prescriptions
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Drug Therapy, Combination
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Drugs, Chinese Herbal
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pharmacology
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Humans
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Practice Patterns, Physicians'
4.Mechanism of leukemia relapse: novel insights on old problem.
Ke-Fu WU ; Guo-Guang ZHENG ; Xiao-Tong MA ; Yu-Hua SONG ; Xiao-Fan ZHU
Journal of Experimental Hematology 2011;19(3):557-560
Relapse, which puzzled several generations of hematologists, is the bottle-neck of radical treatment for leukemias. The progress of Human Microbiome Project at the beginning of 21st century suggested that human body was a super-organism constituted by the core of human cells and symbiotic microorganisms. The elucidation and characterization of endogenous retrovirus and prion protein suggested the possible effects of co-evolutional microorganisms on human health. Recently, the elucidation of the roles of tunneling nanotubes in intercellular communication and transportation suggested a novel way for cellular communication and transport of oncogenic materials. The role and significance of in vivo cell fusion have been studied in more detail. On the other hand, donor cell leukemia was reported. All of these approaches provide novel insights for studying the mechanism of leukemia relapse. Based on previous work, the authors suggest the hypothesis: there are two possible mechanisms for the relapse of leukemias: the minimal residual disease (MRD) and intercellular transportation of oncogenic materials.
Cell Fusion
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Humans
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Leukemia
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pathology
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Neoplasm, Residual
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pathology
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Recurrence
6.Study of the antiulcer effect of pantoprazole
Ying-Xin WANG ; Xiao-Qiang LI ; Guang-Wu WU ; Shan-Jun QIAN ; Hong LEI ; Chang CAO ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim To evaluate the effects of pantoprazole on various experimental acute ulcer inrats and mice. Methods The model of a gastric ulcer of rats or mice was caused bystree- induced ulcer and ligatel pylurus-induced ulcer. Results & Conclusions At adose of 5, 10, 20 mg? kg-1 of Pantoprazole can markedly decrease the ulcer index ofstree-induced ulcer. Pantoprazole(4, 8, 16 mg? kg -1 ) significantly decrease the areaof ligated pylorus-induced gastric ulcer. It was also found that pantoprazole caninhibit the output of basic gastric acid.
7.Measurement of mass fraction of Sanjie Zhentong capsules with near infrared reflectance spectroscopy.
Guang-ren ZHANG ; Yun WU ; Rui-ting JIN ; Qin WAN ; Jin-feng WU ; Xiao-qian XU ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(16):3204-3208
Sanjie Zhentong capsules were scanned by using a near infrared spectra probe with different drug mass fraction and the spectral information of capsule shells and contents in it were obtained. Then partial least squares (PLS) models were developed for the prediction of mass fraction of Fritillariae Thunbergii Bulbus and Resine draconis in Sanjie Zhentong capsules. The correlation coefficient (r9c)) and root mean standard error( RMSEC) of 0.949 5, 0.958 2 and 4.742 4, 4.135 7. The models obtained correlation coefficient (r(v)) of 0.919 2, 0.936 7 and root mean square error (RMSECV) of 6.158 9, 5.037 3 respectively in the training set. The paired T test analysis of statistics showed that there were no significant difference between predictive values and measure values. The established models reflected a strong prediction performance and can meet the needs of the production.
Capsules
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chemistry
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Drugs, Chinese Herbal
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chemistry
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Least-Squares Analysis
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Spectroscopy, Near-Infrared
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methods
8.Leukemia stem cells and their microenvironment--editorial.
Ke-Fu WU ; Xiao-Tong MA ; Guo-Guang ZHENG ; Yu-Hua SONG
Journal of Experimental Hematology 2007;15(6):1139-1141
As pioneer of tumor stem cell research, leukemia stem cell research has not only important theoretical significance, but also clinical application potential. The survival and development of stem cells are directly impacted by their microenvironment. The research on leukemia stem cells and their microenvironment are now becoming a hot topic. The author presumes that stem cells are a population with heterogenecity and hierarchy; any single cell from the population is difficult to form a clone; the interaction between the leukemia stem cell and its microenvironment can be described by the concept of leukemia stem cell niche. In this article, the leukemia cell population with heterogenecity and hierarchy as well as leukemia stem cell niche were summarized and discussed.
Cell Line, Tumor
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Humans
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Leukemia
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genetics
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pathology
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Neoplastic Stem Cells
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metabolism
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pathology
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Stem Cell Niche
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cytology
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Stromal Cells
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cytology
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immunology
9.Immunological activity of Streptomyces polysaccharide in normal and immunosuppressed mice.
Xiao-yun WEN ; Chao SHI ; Shu-guang WU
Journal of Southern Medical University 2008;28(7):1168-1171
OBJECTIVETo investigate the immunological activity of Streptomyces polysaccharide (SMP) on normal and immunosuppressed mice.
METHODSThe effect of SMP on the proliferating activity of normal mouse splenocytes was tested in the mixed lymphocyte culture, and the changes of peripheral blood T lymphocytes were evaluated with acid a-naphthyl acetate esterase (ANAE) method. The ratio of Lyt2+ and L3T4+ T cell subsets was measured by flow cytometry.
RESULTSSMP stimulated obvious proliferation of mixed lymphocytes, showed protective effects on T lymphocyte and increased the ratio of Lyt2+ and L3T4+ cell subsets to nearly normal level in immunosuppressed mice.
CONCLUSIONSSMP can regulate the immune function in mice.
Animals ; CD4-Positive T-Lymphocytes ; immunology ; Female ; Immunocompromised Host ; immunology ; Lymphocyte Culture Test, Mixed ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Polysaccharides ; immunology ; Spleen ; cytology ; Streptomyces ; chemistry ; immunology ; T-Lymphocytes ; cytology ; immunology
10.Latent infection of human herpes virus in hematopoietic system.
Ke-Fu WU ; Xiao-Tong MA ; Guo-Guang ZHENG ; Yu-Hua SONG
Journal of Experimental Hematology 2008;16(6):1251-1256
Up to date, eight types of human herpes viruses have been identified, all of which are ubiquitous, and usually establish latent infection in the host after primary infection. Since most of the herpes viruses are maintained in an asymptomatic form, they are often neglected. However, under some circumstances, these herpes viruses can cause fatal or severe diseases. Furthermore, the association of herpes viruses with hematopoietic malignancies is attracting researchers' attention. With the extensive development of hematopoietic stem cell and organ transplantation, reports regarding transplantation failure and complication caused by infection of human herpes virus has been increasing. Cytokine storm was firstly suggested as the mechanism of graft-versus-host diseases. In recent years, which has also been applied in the pathogenesis research of inflammation, and is supposed to play an important role in severe virus infection. In this paper, through discussing the possible role of latent infection of human herpes virus in the failure or complication of bone marrow or hematopoietic stem cell transplantation, and in refractory leukemia, the function and significance of latent infection of human herpes virus and the cytokine storm it caused were investigated.
Cytokines
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immunology
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Hematopoietic System
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immunology
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virology
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Herpesviridae Infections
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Humans
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Virus Latency