Blood Glucose ; analysis ; Diabetes Complications ; prevention & control ; Diabetes Mellitus, Type 2 ; blood ; therapy ; Humans ; Risk Factors

Objective:To observe the clinical efficacy of tuina therapy in the treatment of cervical spondyIosis of vertebral artery type.Methods:One hundred and fifty-seven cases of cervical spondylosis of vertebral artery type were treated by tuina therapy,three times a week.Ten treatments made up one course and one to two courses of treatment Was performed.During tuina treatment,other modalities that may affect tuina effects were discontinued.The patients were surveyed three months after tuina treatment.Results:Among the 157 cases,109 cases obtained marked effectiveness,26 cases improvement and 6 cases inefiectiveness;16 cases were not surveyed.The total effective rate was 86.6%.Conclusion:Tuina therapy has excellent effects on cervical spondylosis of vertebral artery type.

OBJECTIVE To obtain an effective and feasible supervision method of clinical application of antibacterial drugs.METHODS The clinical application of antibacterial drugs in this hospital was analyzed and calculated(periodically) by their/kinds,quantity and price.Then a price curve was obtained by Excel Table according to(individual) clinical department.RESULTS The expenditure of antibacterial drugs more than 30.0-(40.0) yuan/g was accounted for 24%;the price more than (40.0 yuan/g) was accounted for 6%;and the average(expenditure) per(patient) was 673.45 yuan before the supervision method was taken.The expenditure of(antibacterial) drugs more than 30.0-40.0 yuan/g was accounted for 9%;the price more than 40.0 yuan/g was accounted for 1.8%;and the average expenditure per patient was 264.65 yuan after the supervision method was taken.CONCLUSIONS The(supervision) method for the clinical application of antibacterial drugs is(feasible) and effective.It can be recommended to other hospitals as a controlling method of clinical application of(antibacterial) drugs.

Objective To study the mechanism of extracellular signal-regulated kinase (ERK) pathway inhibitor U0126 on cell cycle of K562 cell lines. Methods The mRNA and protein expressions of ERK, Cyclin D2, Cyclin E in and P27 K562 cell lines after 10 ?mol/L U0126 treatment for 12, 24 and 72 h were detected by RT-PCR and Western blot, respectively. The cell cycle was determined by flow cytometry. Results The mRNA and protein expressions of ERK, Cyclin D2 and Cyclin E in K562 cell lines after U0126 treatment were decreased, while those of P27 were increased. The percentage of cells in G0/G1 phase was increased and that in S phase was decreased. All data mentioned above showed significant difference between the K562 cell lines before and after U0126 treatment(P

The study of relationship between Doctor and Patient is a classical one in the field of medical ethics. Different countries have different cultures, national mentalities, social systems and health care systems. Therefore, the Doctor-Patient relationship differs. Knowing the differences, we can use for references. Based on this, the paper did a comparative research between China and Britain under the background of modern health care system.

OBJECTIVE:To establish an HPLC method for the determination of reserpine in Reserpine injection.METHODS:The determination was performed on Venusil XBP-C18 column with column temperature at 28 ℃.The mobile phase consisted of methanol-water (52:48,pH value of (3?0.2)) with flow rate of 1 mL?min-1.The detection wavelength was 268 nm and sample size was 10 ?L.RESULTS:The linear range of reserpine was 4～l6 ?g?mL-1 (r=0.999 1) with average recovery of 99.69% (RSD=0.98%,n=9).CONCLUSION:The method is accurate,reliable and reproducible for content determination of Reserpine injection.

Objective To evaluate the decompression performance of anti pressure ulcer cushions with different materials and structures. Methods The common cotton cushion, sponge cushion, 3D fabric cushion, and upper 3D fabric and lower sponge cushion were analyzed by XSensor pressure sensor testing system. The pressure distribution, the pressure peak and the average pressure were analyzed to compare the decompression performance and the comfortable capability. Results and Conclusion The upper 3D fabric and lower sponge cushion can en-sure the decompression performance with excellent permeability. Both decompression and air comfort permeability should be the future di-rection of ulcer cushions research and production.

BACKGROUND:Various gene-delivery strategies have be used to transfer targeted genes into damaged bone tissues. As the most efficient gene vector, viral delivery systems have been used in bone tissue engineering research. OBJECTIVE: To thoroughly discuss the applications of different viral vectors in gene-enhanced bone tissue engineering. METHODS:A computer-based online search was performed in PubMed database for the related articles from January 2002 to January 2015. This review centered on viral vector transduction methods and their use in bone tissue engineering. Adenovirus, retrovirus, adeno-associated virus and chimeric virus were al discussed. Advantages and limitations of different vectors and their applicability toward bone tissue engineering were presented in this article. A total of 24 articles were included for review. RESULTS AND CONCLUSION:Current viral vectors for gene delivery in gene-enhanced bone tissue engineering are summarized, including recent work where combinatorial gene therapy is used to express groups of genes to stimulate bone regeneration. Future directions for this field are discussed, where viral vectors mediated gene expression systems wil be combined with cels such as mesenchymal stem cels seeded in synthetic scaffolds to repair bone loss. Gene-enhanced bone tissue engineering has more advantages than traditional tissue engineering; viral vectors contribute to higher gene transfection efficiency than normal vectors. Long-term clinical observation is needed for the safety of viral vectors used in gene-enhanced tissue engineering in the body. Viruses are stil the most efficient means by which exogenous genes can be introduced into seeds cels.

Periodontitis is a chronic infective disease characterized as the destruction of the supporting tissues of the teeth. Bone marrow mesenchymal stem cells, which are ideal adult stem cells for the regeneration of supporting tissues, may play important roles in restoring the structure and function of the periodontium and in promoting the treatment of periodontal disease. As a consequence, the characteristics, especially osteogenic differentiation mechanism, of these stem cells have been extensively investigated. The regulation of the physiological behavior of these stem cells is associated with BMAL1 gene. This gene is a potential treatment target for periodontal disease, although the specific mechanism remains inconclusive. This study aimed to describe the characteristics of BMAL1 gene and its ability to regulate the osteogenic differentiation of stem cells.
ARNTL Transcription Factors ; genetics ; Adult ; Adult Stem Cells ; Bone Marrow Cells ; physiology ; Cell Differentiation ; Hematopoietic Stem Cells ; Humans ; Mesenchymal Stromal Cells ; physiology ; Osteogenesis ; physiology ; Periodontal Ligament ; Periodontitis ; Periodontium ; Regeneration ; Tooth

Adult ; Echinococcosis ; Heart Diseases ; parasitology ; Humans ; Male