Pleiotropic effects of a drug are actions other than that for which the agents were developed.These effects may be desired or undesired,and may be unrelated to their primary mechanism.Statins were designed as a drug for reducing cholesterol,but now pleiotropic effect are found including improvement of endothelial dysfunction,inhancement of eNOS bioavailability antioxide effect,antiinflammatory properties and reduction of hypertension.Understanding those effects is important for treatment and prevention of cardiovascular disease.

Autoantibodies ; Child ; Complement Factor H ; immunology ; Female ; Hemolytic-Uremic Syndrome ; immunology ; Humans

Humans ; Lymphocytes ; cytology ; drug effects ; Micronucleus Tests ; Occupational Exposure ; prevention & control ; Polycyclic Aromatic Hydrocarbons ; toxicity ; Population Surveillance ; methods

Humans ; Intestinal Diseases ; physiopathology ; Sepsis ; physiopathology

Objective: To evaluate the quality of Zidan Huoxue Tablets (ZHT) based on HPLC fingerprint and multi-component content determination combined with chemical pattern recognition method. Methods: ACE Neptune-C18 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 275 nm and the column temperature was 25 ℃. A total of 18 batches of ZHT was analyzed, and the quality of ZHT was evaluated by the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints combined with cluster analysis and principal component analysis. Results: The fingerprint of ZHT was established. Twenty-six common peaks were identified and nine of them were identified, including 2-sodium danshensu, 8-rosmarinic acid, 9-lithospermic acid, 10-salvianolic acid B, 12-salvianolic acid A, 21-dihydrotanshinone I, 22-cryptotanshinone, 23-tanshinone I, and 25-tanshinone IIA. The similarity of fingerprints of 18 batches of ZHT was between 0.93 and 1.00. The results of cluster analysis and principal component analysis were basically consistent with similarity results. After validating the multiple component quantitative analysis condition through methodology, the average recoveries were between 98.27% and 103.42%, and the RSD were in the range of 0.86%-2.53%. The content of sodium danshensu, rosmarinic acid, lithospermic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA in 18 batches of ZHT were in the range of 0.149%-0.218%, 0.179%-0.225%, 0.222%-0.286%, 3.570%-6.399%, 0.048%-0.136%, 0.122%-0.309%, 0.061%-0.215%, 0.093%-0.413%, respectively. Conclusion: There is a certain quality difference between different batches of ZHT. Through the combination of fingerprinting, cluster analysis and principal component analysis, the quality of ZHT can be comprehensively evaluated. The establishment of this method can provide reference for the quality control and evaluation of ZHT.

ObjectiveTo summarize the recent therapeutic effects of mesh-plug tension-free hernioplasty in elderly inguinal hernia. MethodsAll cases were performed with mesh-plug case-hardened products, which are manufactured by American Bard Company. ResultsAll the operative procedures were performed smoothly. Postoperative complications were:6 cases of urinary retention,4 scrotal hydroceles,2 persistent wound pain,2 local lightly projections with foreign body sensation,2 hypoincisional haematoma. 1～24 months of follow-up were taken,2 recent recurrences were reported. ConclusionMesh-plug tension-free hernioplasty is a perfect surgical operation. Its main characteristics are:simple performance, less trauma, tension-free, time-saving, good recent therapeutic effect, and fewer recent recurrence. It is especially suitable to the elderly patients and/or Patients with other diseases.

Stroke is characterized by a group of acute cerebral vascular diseases which attack acutely with focal neurological deficits. Residual motor deficits often sojourn after stroke. Cortical stimulation, which is a technique developed many decades ago, has recently re-emerged as a promising method for researchers in their quest to causally probe cortical representations of sensorimotor and cognitive functions and to facilitate the treatment of various neuropsychiatric disorders. The article summarizes the research progress of cortical stimulation in the promotion of motor function recovery after stroke, the method of operation, the possible mechanisms and the prospect.

Objective To study the antifatigue effect of sea cucumber exstract.Methods According to the experimental methods of animal antifatigue, three doses of sea cucumber extract were applied to mice and their antifatigue effects were compared statistically.Result 300 mg?kg -1 ?d -1 sea cucumber extract could significantly prolong pole climbing time in mice, increase the reserves of liver glycogen and the content of blood glucose, decrease the contents of blood urea nitrogen (BUN) and blood lactic acid after strenuous exercise. Conclusion The preliminary study showed that the sea cucumber exstract has some antifatigue effects.

AIM To examine the role of Cl - channels in endothelin 1 (ET 1) induced proliferation of vascular smooth muscle cells (VSMCs). METHODS Through cell counting and 3H TdR incorporation, together with [Ca 2+ ] i measurement by fura 2/AM fluorescence, we studied the effects of Cl - channel blockers on proliferation of VSMCs induced by ET 1. RESULTS Cl - channel blocker, DIDS, inhibited 10 nmol?L -1 ET 1 induced proliferation of VSMCs in a concentration dependent manner, however,other Cl - channel blockers such as IAA 94, NPPB, SITS, DPC and furosemide, all failed to inhibit ET 1 induced proliferation of VSMCs. DIDS reduced 10 nmol?L -1 ET 1-induced sustained [Ca 2+ ] i elevation but not Ca 2+ release from internal stores; Pretreatment of cells with 1 ?mol?L -1 nifedipine abolished the inhibitory effect of 3 ?mol?L -1 DIDS on proliferation of VSMCs and [Ca 2+ ] i elevation evoked by 10 nmol?L -1 ET 1 , while after cells were pretreated with 10 ?mol?L -1 SK&F96365, the same concentration of DIDS could also inhibit the aforecited effects of ET 1;3 ?mol?L -1 DIDS had no effect on 30 mmol?L -1 KCl induced elevation of [Ca 2+ ] i. CONCLUSION DIDS inhibited Ca 2+ influx and VSMCs proliferation induced by ET 1 through blockade of DIDS sensitive Cl - channels, and these channels may play important roles in modulating Ca 2+ influx and VSMCs proliferation induced by ET 1.

AIM To investigate the feasibility of bacterial endotoxins test for cisplatin injection. METHODS Gel clot and kinetic turbidimetric limulus tests were applied. RESULTS After diluted to 0 14 g?L -1 ,six batches of cisplatin injection didn't interfere with the test for bacterial endotoxins, others except three also didn't interfere with the test at the concentration of 0 28 g?L -1 ; The content of bacterial endotoxins in all samples tested was not more than 1 8 ZU?g -1 . CONCLUSION The kinetic turbidimetric limulus test provides a new way to detect the content of endotoxins in cisplatin injection, samples can be diluted to 0 14 g?L -1 to refute their interfering effects on routine bacterial endotoxins test.