2.Analysis of photosynthetic characteristics and its influencing factors of medicinal plant Mirabilis himalaica.
Qi-Qiang GUO ; Hong QUAN ; Xiao-Zhong LAN ; Lian-Qiang LI ; Hui-E LI
China Journal of Chinese Materia Medica 2014;39(14):2769-2773
To study photosynthetic characteristics and its influencing factors in leaves of medicinal plant Mirabilis himalaica, and provide an evidence for guiding artificial planting and improving the quantity. The light-response and diurnal photosynthesis course of leaves at the booting stages of 1-3 year old M. himalaica were measured with LI-6400 system. The Results showed that the light response curves were fitted well by non rectangle hyperbola equation (R2 > or = 0.98). The values of the maximum photosynthetic rate (Pmax) and light use efficiency of three-year old M. himalaica leaves were higher than those of 1-2 year old individuals. The diurnal variation of net photosynthetic rate (Pn) and stomatal conductance (Gs) of 2-3 year old M. himalaica were typical double-peak curves determinately regulated by stomatal conductance. However, transpiration rate (Tr) of 1-3 year old plants leaves were single-peak curve, which was self-protection of harm reduction caused by the higher temperature at noontime. Correlation analysis showed that the changes between photosynthetic active radiation (PFD), air temperature (T ) and Pn, were significant positive related. Therefore, M. himalaica is a typical sun plant, which should be planted under the sufficient sunshine field and prolong the growing ages suitably in order to improve the yield.
Breeding
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Mirabilis
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growth & development
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metabolism
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Models, Biological
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Photosynthesis
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Temperature
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Time Factors
4.Clinical Significance of Fecal Smear Examination on Diagnosing Intestinal Flora Imbalance
xiao-ming, WANG ; rui-qin, ZHAO ; su-xia, XIN ; hong-e, SONG ; ying-hui, GUO ; ge-lan, BAI
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To study the clinical significance of fecal smear examination on diagnosing intestinal flora imbalance in infantile diarrhea.Methods A sterile cotton swab was used to spread a layer of fresh feces quantum satis from a sterile container on a clean slide;the smear was fixed and stained with Gram′s methods after it was air-dried,then the specimen was observed with a microscopy(field lens 100 ? eye lens 10) and recorded.Results In the acute diarrhea group(40 cases),the distribution feature of 3 floras on the fecal smears:≥50% Gram-negative bacilli in 6 cases(15%),≥50% Gram-positive cocci in 30 cases(75%) and ≥68% Gram-positive bacilli in 4 cases(10%).In the delayed and chronic diarrhea group(62 cases),the distribution feature of 3 floras on the fecal smears:≥50% Gram-negative bacilli in 7 cases(11.29%),≥50% Gram-positive cocci in 44 cases(70.97%) and ≥68% Gram-positive bacilli in 6 cases(9.68%).In the normal control group(32 cases),the distribution feature of 3 floras on the fecal smears:≥50% Gram-negative bacilli in 1 case(3.13%),≥50% Gram-positive cocci in 1 case(3.13%),and ≥68% Gram-positive bacilli in 17 cases(53.13%).For the distribution of 3 floras in the 3 groups,chi-squared test was performed,and the results showed that the difference was significant in Gram-positive cocci(?~2=47.76 P0.05).Conclusions Acute,delayed or chronic diarrhea can lead to flora imbalance.In order to timely and rapidly know the flora imbalance in children with diarrhea,clinically the simple,easily operated and practical smear staining method shall be widely applied.
6.Nanometer detection of self-assembly morphology of sodium hyaluronate injection on mica.
Mu-dan HE ; Tian-xing XU ; Xiao-ping WANG ; Ya-lan LI ; Yu-yong LIU ; Shan-e DUAN
Journal of Southern Medical University 2010;30(12):2637-2639
OBJECTIVETo detect the self-assembly morphology of sodium hyaluronate injection on mica using atomic force microscopy(AFM).
METHODSAtomic force microscopy with nanometer resolution was used to observe the self-assembly morphology of different concentrations of sodium hyaluronate injection on mica at room temperature.
RESULTSThe self-assembly morphology of 0.001, 0.01, and 0.1 mg/ml sodium hyaluronate injection on mica featured piebald, reticular and dendritic structures, respectively. At 1 and 5 mg/ml, sodium hyaluronate injection displayed bacilliform and spherical structures on mica, respectively; the diameter and height of the particles of 5 mg/ml sodium hyaluronate was 197.97±78.48 nm and 30.79±18.67 nm, significantly greater than those of 0.1 mg/ml sodium hyaluronate injection (49.52±11.93 nm and 5.37±1.59 nm, respectively, P<0.05).
CONCLUSIONThe self-assembly morphology of sodium hyaluronate injection on mica varies with its concentration. The piebald and reticular structure may facilitate the function of sodium hyaluronate, and the dendritic feature resembles the representative model of diffusion-limited aggregation (DLA).
Aluminum Silicates ; chemistry ; Hyaluronic Acid ; administration & dosage ; chemical synthesis ; chemistry ; Microscopy, Atomic Force ; Nanostructures ; Surface Properties
7.Studies on chemical constituents from twigs of Morus atropurpurea.
Yan-Lan XU ; Xu-E LI ; Yu-Xiao ZOU ; Ji-Jun CHEN
China Journal of Chinese Materia Medica 2008;33(21):2499-2502
OBJECTIVETo study the chemical constituents of the twigs of Morus atropurpurea.
METHODThe compounds of the EtOAc fraction were isolated and purified by column chromatography on silica gel, polyamide, Sephadex LH -20, and their structures were elucidated on the basis of spectroscopic evidence (MS, NMR).
RESULTEleven compounds were identified as mulberrin (1), cyclomulberrin (2), morusin (3), cyclomorusin (4), 2', 4',4, 2"-tetrahydroxy-3'-{3"-methylbut-3"-enyl-}-chalcone (5), mulberrofran G (6), scopoletin (7), moruchalcone A (8), kaempferol (9), ursolic acid (10), beta-daucosterol (11).
CONCLUSIONExcept compounds 9 and 11, all the other compounds were obtained from M. atropurpurea for the first time.
Flavonoids ; chemistry ; Kaempferols ; chemistry ; Magnetic Resonance Spectroscopy ; Morus ; chemistry ; Phytosterols ; chemistry ; Plant Stems ; chemistry ; Scopoletin ; chemistry ; Triterpenes ; chemistry
8.Dynamic monitoring of occupational hazards exposure level and evaluation the effects of prevention measures at a sentinel factory.
Jing LIU ; Yan-rang WANG ; Jin-yan SUN ; Xiao-dan XUE ; Shu-lan ZHAO ; Mei-li LI ; Jian ZHANG ; Wan-chao ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(12):919-921
9.Reaffirmation and classifcation of the areas with highwater iodine and the endemíc areas of íodine excess goiter in Jiansu Provice
Pei-hua, WANG ; Qing-lan, ZHANG ; Yong-lin, ZHOU ; Zhi-gao, CHEN ; Ying-xia, HE ; LIANG-PING ; Yue-e, CHEN ; Ming, WU ; Xiao-shu, HU
Chinese Journal of Endemiology 2008;27(6):657-659
Objective To affirm and calssify the arsas with high water iodine and the endemic areas of indline excess goiter in Jiangsu Privince accirding to mational standard.Methods A cross section survey was condueted in 2005 at township level in Fenxian,Tongshan,Suining,Pizhou counties in Xuzhou municipal and Chuzhou district in Huai'an municipal in Jiangsu Province One sample of dringing well water from five directions in the five villages located dircetions of every township namely east west south north and central.was tested for its water iodine concentration.If the sample number sas less than 5 in one village,then all the well water would be tested Endemic stutas of iodine excess goiter was investigated in those townships whose menian water iodine comcentration was between 150 to 300 μg/L Then status was affirmed and reclassifed according.to National Criteria GB/T 19280-2003 Results In all 158 tawnships from the 6 counties the median of water iodine concentration in 79 townships were over 150 μg/L with 32 townships in the range of 150 to 300μg/L In those 32 townships 9 met the criteria of area of high water iodine 23 accorded with that of encemic areas of iosine excess goiter 16 had stopped the supply of non-iodzed salt in advace,but 16 newly detected areas were still served with iodized salt Four were 300 μg/L Conctusions Iodized salt intervention should be stopped in all townships with the problems of high water iodine and the endmic areas of iodine excess goter in order to prevent the possible hazards due to double intade.
10.Survey on awareness of knowledge related to hepatitis B among fetation or puerpera in Luohu district of Shenzhen city
Yu-Rong JIANG ; Hai-Hong SU ; Xiao-E ZHANG ; Yan-Qin LAN ; Jing JIANG
Chinese Journal of Modern Nursing 2009;15(22):2115-2117
ry for different pregnant women or puerperal population, to enlarge the coverage rate of hepatitis B knowledge.