ObjectiveTo observe the effect of vinpocetine sustained-release capsules on post-stroke depression and recovery of neurological function. MethodsA total of 64 patients with acute post-stroke depression were randomly divided into Vinpocetine sustained-release capsules group( 34 cases) and control group(30 cases). Conventional therapy was given to both groups. To the treatment group, vinpocetine sustained-release capsules was given at the same time. Depression status was assessed with Hamilton Rating Depression Scale(HAMD). Meanwhile ,Mimi-Mental State Examination( MMSE), National Institutes of Health Stroke Scale(NIHSS) and Activities of Daily Living(ADL)were given in to both groups. ResultsAfter 6 to 12 weeks' therapy, the total score of HAMD, NIHSS and ADL of Vinpoeetine group were significantly lower than the control group ( all P ＜ 0.05 ), MMSE score increased significantly ( P ＜ 0.05 ) in Vinpocetine group. The curative rate and effective rate of Vinpocetine group were 38％ and 91％. It was significantly higher than the 20％ and 73％ of the control group ( all P ＜ 0.05 ). ConclusionVinpocetine sustainedrelease capsules was effective to treat post-stroke depression. It could improve the emotional state of patients and promote the recovery of neurological function.

Objective To observe the effect of vinpocetine on monoamine transmitters in cerebral of poststroke depression (PSD)rats and to investigate the mechanism of pharmacotherapeutics on PSD. Methods Rats were divided randomly into the sham-operated group,the normal saline group,the low dose of vinpocetine group and the high dose of vinpocetine group. Giving the left middle cerebral artery occlusion, the PSD rat model was established by unexpected chronic mild stress. When the PSD rat model was established, vinpocetine group was given vinpocetine(5mg/kg, 10mg/kg) and the normal saline group was given normal saline. Then the ethological score of depression was evaluated on 14 and 28 days. The monoamine transmitter in the frontal cortex and hippocampus and brainstem were detected by the fluorospectrophotometry. Results On the 28th day after the model establishment,compared with the normal saline group, the ethological score of depression level was decreased obviously. Compared with the normal saline group, vinpocetine could improve the ethological score of depression level of the PSD rat model, and these concentrations of 5-hydoxytrypatarmine ( 5-HT), noradrenalin ( NE ) and dopamine ( DA ) in the frontal cortex, hippocampus and brainstem. The level of NE ( ( 192.4 ± 34.8 ) ng/g, ( 206. 0 ± 41.7 ) ng/g,(91.1 ±23.0) ng/g] ,5-HT( (494. 1 ± 50.7) ng/g, (599.7 ± 39.2) ng/g, (541.7 ± 62.6) ng/g) and DA ( ( 298.6 ± 32.6) ng/g, ( 297.0 ± 38.1 ) ng/g, ( 85.9 ± 24.3 ) ng/g) in the high dose of vinpocetine group were significantly higher than that in the normal saline group ( NE (92.4 ± 17.5 ) ng/g, ( 131.4 ± 34.8 ) ng/g, (49.0 ±13.6)ng/g;5-HT(367.8 ±87.3) ng/g,(498.7 ± 79.6) ng/g, (320.4 ±59.4) ng/g; DA( 106.1 ±23.0)ng/g,(97.0 ±21.7)ng/g, (50.4 ± 13.8 )ng/g)(P ＜ 0.01 ). There were increased obviously by the high dose than the low dose of vinpocetine group. Conclusion Vinpocetine could treat PSD by increasing the level of monoamine transmitters in PSD rats' brain.

Child ; Communicable Diseases ; epidemiology ; Humans ; Nutritional Status ; Zinc ; deficiency

Drugs, Chinese Herbal ; pharmacology ; Humans ; Kidney Failure, Chronic ; mortality ; physiopathology ; Renal Dialysis ; methods ; mortality ; Survival Rate

Choristoma ; Ear Diseases ; Female ; Glioma ; Humans ; Mastoid ; Middle Aged

To further analyze immunoglobulin (Ig) properties of panel reactive antibodies (PRA) and their potential harmful effects on allogeneic renal transplants. Methods Since dithiothreitol (DTT) is able to crack disulphuric bond of Ig and to depoly-merize IgM with heavy molecular weight, PRA study was performed with treatment of DTT. Thus, a DTT-ERA method was established, which can identify immunoglobulin class in sera of high PRA patients. Results Among 701 recipients, whose sera were positive by the standard PRA, the positive rate of DTT-PRA was 33.2% . Besides, positive PRA patients could be divided into three groups according to their sera's sensitivity to DTT: Group 1 consisted of patients whose sera contained exclusively IgM antibodies; Group 2 consisted of patients whose sera contained of IgG antibodies only; Group 3 consisted of patients whose sera were found to contain both IgM class antibodies and IgG antibodies. Conclusion IgM class antibodies are not associated with posttrans-plant rejection, while IgG antibodies alone and mixture of IgG and IgM antibodies may mediate acute, or even hyperacute rejection.

OBJECTIVE:To establish a method for detecting bacterial endotoxins in methylthioninium chloride injection (MCI) quantitatively. METHODS: Kinetic turbidimetric limulus test was applied to detect bacterial endotoxins in MCI quantitatively, and compared with gel-clot method. RESULTS: MCI was un-interfered with the test for bacterial endotoxins at the concentration of 0.125 mg?mL-1; the content of bacterial endotoxins in all samples tested (10 mg?mL-1) were not more than 0.25 EU?mg -1, which were in accordance with the result of gel-clot method. CONCLUSIONS: Kinetic turbidimetric limulus test provides a new way to detect bacterial endotoxins in MCI quantitatively.

OBJECTIVE:To study the absorptive effect of activated carbon and desorptive effect of organic solvent on endotoxin.METHOD:The effect of different pretreatment modes,time,temperature,amount of activated carbon as well as treatment time on the absorptive effect of activated carbon were analyzed using limulus reagent test.Different organic solvents and treatment time were used to study the desorptive effect of organic solvent on endotoxin.RESULTS:Activated carbon was treated by high temperature baking or adding 0.5 mol?L-1 NaOH represented same effect in removing endotoxin.It showed that optimal time for absorption of endotoxin was 12 min and the amount of activated carbon was at 1.5%.The absorptive effect was similar under condition of room temperature and 37 ℃.The best effect of desorption achieved by using 75% alcohol as desorption reagent after 8 minutes of shaking.CONCLUSION:The activated carbon could be used to absorb endotoxin effectively under suitable conditions and organic solvent could be used to desorb endotoxin from activated carbon.

OBJECTIVE: To investigate the effect of detection index of IL-4 and IgE on rapid examination of drug-induced allergic reaction.METHODS: The guinea pigs were injected with drugs intravenously and intraperitoneously,such as normal saline,horse serum,Qingkailing injection,Xiangdan injection,cerebrolysin injection and Chuankezhi injection once a day for 3 days.Then the content of IL-4 and IgE in serum of guinea pigs was recorded at different time points.Allergic reactions of guinea pigs were observed 8 days after intravenous administration,14 days and 21 days after intraperitoneous administration.RESULTS: As compared with normal saline except for Chuankezhi injection,serum level of IL-4 and total IgE were raised obviously before guinea pigs were challenged either in 8 days after intravenous injection of other drugs or in 14 days and 21 days after intraperitoneous injection.Allergic reaction occurred on all those guinea pigs after they were challenged.CONCLUSION: The detection of IL-4 and IgE provides a new way for rapid test of drug-induced allergic reaction.

OBJECTIVETo establish a method to produce virus-like particles (VLP) of poliovirus type I in Saccharomy cescerevisiae to develop potential novel recombinant vaccine against poliovirus type 1.

METHODSThe genes of P1 and 3CD of poliovirus type I were optimized, synthesized and inserted into expression vector, which was further transfected into Saccharomy cescerevisiae. The extracts of yeast cells were purified by CsCl density gradient centrifugation after induction and cell lysis.

RESULTSElectrophoresis and sequencing analyses showed that the genes P1 and 3CD of poliovirus type I were successfully inserted into expression vector and encode a protein whose amino acid sequences were identical with wide-type genes of poliovirus type I. Electronic microscopy analysis showed that the VLPs of poliovirus type I could be efficiently formed in Saccharomy cescerevisiae.

CONCLUSIONThe VLPs of poliovirus type I could be efficiently produced by co-expression of P1 and 3CD genes in Saccharomy cescerevisiae.

Female ; Gene Expression ; Humans ; Male ; Poliomyelitis ; prevention & control ; virology ; Poliovirus ; genetics ; metabolism ; Poliovirus Vaccines ; genetics ; metabolism ; Saccharomyces cerevisiae ; genetics ; metabolism ; Viral Proteins ; genetics ; metabolism ; Virion ; genetics ; metabolism