Objective To investigate the clinical significance of combined detection of serum pepsinogen (PG) and anti-Hp IgG antibody in early screening for gastric ulcer .Methods 65 patients confirmed as gastric ulcer for the first time were selected as ulcer group ,while 65 healthy people in the same period as the control group .Transmission detection using latex enhanced immune nephe-lometry was adopted to detect their serum PG Ⅰ ,PG Ⅱ and the PG Ⅰ /PG Ⅱ ratio (PGR) was calculated .Enzyme-linked immu-nosorbent assay(ELISA) was used to detect serum anti-Hp IgG antibody .Results The average concentration of serum PG Ⅰand PG Ⅱof patients in the ulcer group were (167 .14 ± 22 .43) ,(26 .68 ± 11 .24) ng/mL ,respectively ,and PGR was 8 .07 ± 1 .19 .The average concentration of serum PG Ⅰand PG Ⅱof subjects in the control group were (52 .15 ± 20 .17) ,(11 .26 ± 7 .68) ng/mL ,re-spectively ,and PGR was 3 .48 ± 1 .92 .The differences of PGⅠ ,PGⅡand PGR between the two group showed statistical significance (P<0 .05) .The positive rate of serum anti-Hp IgG antibody of patients in the ulcer group was 80 .5% ,which was obviously higher than that in the control group(26 .3% )(P<0 .05) .The serum PGⅠ ,PGⅡand PGR of subjects in Hp infection positive group were markedly higher than those in the negative group(P<0 .05) .Conclusion Combined detection of serum PG and anti-Hp IgG anti-body may be used as an screening indicators for early gastric ulcer .

Objective To investigate the relationship between Helicobacter pylori (H. pylori) infection and concentration of reactive oxygen species (ROS) in AGS cells. Methods AGS cells were cultured with either Hp11638 (CagA~+ , VacA~+ ) extract or Hp11638 mutant (CagA~+ , VacA~-) extract for 48 hours, then the cells and supernatants were collected. The concentration of ROS in AGS cells was measured by flow cytometry. The eytochrome C reduction was detected by spectrophotometer at 550 nm. Results The ROS levels in the AGS cells were correlated with two H. pylori strains in a concentration- and time-dependent manner. The ROS levels in AGS cells treated with Hp11638 extract in different concentrations or times were correspondingly higher than those treated with Hp11638 mutant extract. Similar results were found in examination of cytochrome C reduction. Conclusion The elevation of ROS in AGS cells is related to effects of H. pylori proteins, and the VaeA protein involves in the process.

Objective:To clone the cDNA in full-length of human mammaglobin,do prokaryotic expression and purify hMAM protein,as a basis for early diagnosis of breast cancer.Methods :hMAM cDNA was amplified through RT-PCR from breast cancer tissue and breast cancer cell line MD-MB453,and the recombination pQE40-hMAM vector was constructed and expressed in E.Coli.M15 after induction by IPTG.The fusion protein was purified with Ni-NTA-His affinity chromatography. Results: Two subtypes of hMAM cDNA and the hMAM(Isoform) cDNA were found,in which consisted of 270 bp,different from the wildtype hMAM cDNA of 279 bp on nine continuous base pair missing.The fusion protein formed inclusion body in prokaryotic expression system and the renatured protein was purified which purity was about 97%.Conclusion: The recombinant hMAM was successfully purified.

【Objective】 To study the characteristics of HIV infection among voluntary blood donors in Shijiazhuang from 2011 to 2021, so as to provide reference for decision making in the control of HIV for blood centers. 【Methods】 The confirmatory results of HIV reactive samples in initial screening among voluntary blood donors from 2011 to 2021 in our center were statistical analyzed. 【Results】 A total of 2 008 299 samples from 1 667 315 blood donors were detected, among which 3 217 samples were HIV reactive and 234 were confirmed positive, with the positive rate at 11.65/100 000 and the prevalence of 14.03/100 000. The prevalence in men was higher than that in women (16.52/100 000 vs 1.39/100 000), in first-time blood donors higher than that in repeated donors (17.27/100 000 vs 8.12/100 000), in whole blood donors higher than that in plateletpheresis donors (12.01/100 000 vs 8.41/100 000), and the differences were statistically significant (P<0.05). The male homosexual transmission was the main routes of transmission, accounting for 62.39% (146/234). And 72% of double-reagent reactive samples were confirmed positive. Four samples were screened in the serological window period and 6 samples were from HIV positive confirmed donors. 【Conclusion】 The HIV prevalence among voluntary blood donors in Shijiazhuang was low. A certain percentage of repeated blood donors got newly infected. NAT could shorten the detection window period of HIV. Since some confirmed positive samples had not been detected out by NAT, publicity and education should be strengthened to reduce the probability of infected or high-risk groups to participate in blood donation.

With the improvements in surgical technique,the indications for liver transplantation(LT)are being expanded to more complex and severe patients,who not only have liver function in the terminal stage,but also extrahepatic organs involve of varying degrees dysfunction.It was demonstrated that the strategy of extrahepatic organs management in early period after LT was associated with improving recovery.This paper summarized the strategy of extrahepatic organs management in early period after adult LT.Continuing to monitor system hemodynamics is helpful for stable graft perfusion.It is necessary to manage derangements of preload,myocardial contractility,and afterload.Early postoperative extubation is recommended by current guidelines.Routine screening pulmonary complications may help weaning from the ventilator.The diagnosis of acute kidney injury(AKI)after LT has been reported to using Kidney Disease:Improving Global Outcomes(KDIGO)criteria,a delayed calcineurin inhibitor initiation strategy may be beneficial for renal protection.Currently,there is no consensus on timing of renal replacement therapy initiation in patients with AKI.Patients with cirrhosis and a history of hepatic encephalopathy(HE)displays impaired neurological recovery,the diagnosis of HE is crucial and challenging.