Objective To understand the epidemiological features of epidemic cerebrospinal meningitis in Quzhou City,and to provide scientific evidence for formulation of relevant prevention and control measures.Methods The descriptive epidemiological method was applied to the analysis of the epidemiological features of epidemic cerebrospinal meningitis in Quzhou city from 1950 to 2006.Results The epidemic cerebrospinal meningitis reported a total of 30 294 cases including 1 455 deaths.The incidence rate of epidemic cerebrospinal meningitis was 28.11/100 000 on average per year,and the fatality rate of epidemic cerebrospinal meningitis was 4.80%.There were cases reported all-year with a higher occurrence in winter and spring and the highest occurrence in March accounting for 42.85% of the total of cases.The cases were mainly in children under 15 years concentrating on those living scatteredly or in nursery,who accounted for 36.60% of the total of cases.Conclusions It is necessary to enhance surveillance and the forecast of epidemic cerebrospinal meningitis,and concerning the change in the epidemic virus groups,to ensure routine preventive vaccination.It is signficant to control the occurrences and epidemics of epidemic cerebrospinal meningitis.

BACKGROUND: Mesenchymal stem cell (MSC)-based cell transplantation is an effective means of treating chronic liver injury, fibrosis and end-stage liver disease. However, extensive studies have found that only a small number of transplanted cells migrate to the site of injury or lesion, and repair efficacy is very limited. METHODS: Bone marrow-derived MSCs (BM-MSCs) were generated that overexpressed the erythropoietin (EPO) gene using a lentivirus. Cell Counting Kit-8 was used to detect the viability of BM-MSCs after overexpressing EPO. Cell migration and apoptosis were verified using Boyden chamber and flow cytometry, respectively. Finally, the anti-fibrosis efficacy of EPO-MSCs was evaluated in vivo using immunohistochemical analysis. RESULTS: EPO overexpression promoted cell viability and migration of BM-MSCs without inducing apoptosis, and EPO-MSC treatment significantly alleviated liver fibrosis in a carbon tetrachloride (CCl4 ) induced mouse liver fibrosis model. CONCLUSION EPO-MSCs enhance anti-fibrotic efficacy, with higher cell viability and stronger migration ability compared with treatment with BM-MSCs only. These findings support improving the efficiency of MSCs transplantation as a potential therapeutic strategy for liver fibrosis.

BACKGROUND: Mesenchymal stem cell (MSC)-based cell transplantation is an effective means of treating chronic liver injury, fibrosis and end-stage liver disease. However, extensive studies have found that only a small number of transplanted cells migrate to the site of injury or lesion, and repair efficacy is very limited. METHODS: Bone marrow-derived MSCs (BM-MSCs) were generated that overexpressed the erythropoietin (EPO) gene using a lentivirus. Cell Counting Kit-8 was used to detect the viability of BM-MSCs after overexpressing EPO. Cell migration and apoptosis were verified using Boyden chamber and flow cytometry, respectively. Finally, the anti-fibrosis efficacy of EPO-MSCs was evaluated in vivo using immunohistochemical analysis. RESULTS: EPO overexpression promoted cell viability and migration of BM-MSCs without inducing apoptosis, and EPO-MSC treatment significantly alleviated liver fibrosis in a carbon tetrachloride (CCl4 ) induced mouse liver fibrosis model. CONCLUSION EPO-MSCs enhance anti-fibrotic efficacy, with higher cell viability and stronger migration ability compared with treatment with BM-MSCs only. These findings support improving the efficiency of MSCs transplantation as a potential therapeutic strategy for liver fibrosis.