Objective To investigate the effect of laparoscopic hand and whole system cleaning method, and compare with endoscopic cleaning method for one hand before. Methods A total of 100 laparoscopic operation patients in our hospital from January 2012 to December 2013 as the research object, from January to December 2012, hand clean-ing method for cavity mirror one before the establishment group A(n=50), from January to December 2013 for hand supply one, establishment of group B (endoscopic cleaning method,n=50),group A endoscope cleaning hand forone before the group, group B for hand cleaning method for combined cavity mirror, the visual method of the two groups the rate of qualified,qualified rate of a magnifying glass,the qualified rate of occult blood test, the number of colony qualified rate, and the interval time of operation, qualified equipment package were compared between two groups. Results The visual method of group B qualification rate, magnifying glass, the rate of qualified, qualified rate of oc-cult blood test colonies qualified rate was higher than that in group A, with statistically significant difference between two groups(P<0.05). Operation time interval of the group B was significantly shorter in the group A, with statistically significant difference between two groups(P<0.05). qualified equipment package operation of group B was significantly more than group A, with significant difference between two groups (P<0.05). Conclusion The application of cleaning cleaning hands for development of management mode in laparoscopic instruments, is conducive to improving steriliza-tion quality,shorten the operationtime interval,thus greatly improve the efficiency and quality of nursing work.