Objective To investigate the effect of anti-CD81(antibodys against CD81) on the proliferation of astrocytes. Methods Purified astrocytes from newborn rats' cerebral cortex were divided into 6 groups and added with anti-CD81 different concentrations(0,0.1,0.5,1,5,10?mg/L).The activity of astrocytes was tested by methyl thiazolyl terazolium(MTT).Three significative groups were chosen based on MTT result and added with anti-CD81 of different concentrations(0,0.5,5mg/L).After administration for 24 hours,the cell cycle of the astrocytes was measured by flow cytometer.The corresponding data were analyzed with SPSS statistical software. Results 1.By MTT,the average optical density(AOD) values of astrocytes were reduced after administration with anti-CD81 of different concentrations for 24 hours,that is,the number of astrocytes was reduced,which indicated anti-CD81 inhibited the proliferation of astrocytes and the effect showed a dose-dependent pattern.2.By cell cycle analysis,a progressive dose-dependent decrease was found in the index of cells in G-0/G-1 phase and an increase in S phase.Such as,the index of cells in G-0/G-1 phase,was 82.73 in 0,is 82.16 in 0.5?mg/L,was 78.58 in 5?mg/L.Conclusion Anti-CD81 inhibits the proliferation of astrocytes and the number of astrocytes is reduced.Further more,the index of cells decreases in G-0/G-1 phase and increases in phase S after administration with anti-CD81.This study shows that anti-CD81 doesn't restrain the cells from G-1 phase to S phase but the cells are arrested in S phase.

OBJECTIVE: To investigate the effect of yizhi jiannao granule concentration fluid (YCF) on the expression of peptidyl-prolyl-cis-trans isomerase A (Pin1) and high mobility group box 1 (HMGB1) mRNA in the hippocampus of senescence accelerated mice Senile-Prone8(SAMP8). METHODS: Six-month old SAMP8 mice were randomly divided into a YCF group and a model group. Six-month old SAMP8 mice were served as a normal control group. The YCF group was ravaged, while the model group and the normal control group were gavaged with double-distilled water for 8 weeks. The hippocampus was taken out for examination. The expression of Pin1 and HMGB1 mRNA was measured by RT-PCR. RESULTS: In the YCF group, the level of Pin1 mRNA increased, and the level of HMGB1 mRNA decreased compared with that of the model group. CONCLUSION Yizhi jiannao granules can prevent Alzheimer's disease by increasing the Pin1 level and decreasing the HMGB1 level.
Aging ; drug effects ; metabolism ; Alzheimer Disease ; metabolism ; Animals ; Drugs, Chinese Herbal ; pharmacology ; HMGB1 Protein ; genetics ; metabolism ; Hippocampus ; metabolism ; Male ; Mice ; NIMA-Interacting Peptidylprolyl Isomerase ; Peptidylprolyl Isomerase ; genetics ; metabolism ; RNA, Messenger ; genetics ; metabolism

Objective To investigate clinical manifestations and clinical diagnosis and treatment and pathological and immunohistochemical features in gastrointestinal stromal tumors. Methods The clinical data of fifty-two cases with gastrointestinal stromal tumors were collected, whose clinical diagnosis and treat-ment and pathological features were retrospectively analyzed from January 1995 to December 2007. Results All patients received operation therapy, only forty-five cases with complete surgical resection. The immu-nohistochemical staining showed that the cases with CD117 positive accounted for 100% (52/52) and CD34 positive accounted for 88.5% (46/52). Conclusions Surgery was necessary for all patients, especially complete surgical resection. Gastrointestinal stromal tumors were poor in preoperative diagnosis, which diag-nosis was based on the immunohistochemical staining of the tumor tissue. CD117 and CD34 tumor markers may help to diagnose gastrointestinal stromal tumors.

Objective To explore cytotoxic secondary metabolites from a marine actinomycete Nocardiopsis sp .SCSIO 11492 .Methods Isolation and purification were carried out by column chromatography over silica gel ,Sephadex LH-20 ,and ODS structures of the isolates were identified mainly by NMR spectroscopic data .And cytotoxic bioassay was performed using MTT method .Results Five compounds were identified as 2′-deoxyadenosine (1) ,2′-deoxythymidine (2) ,2′-deoxyuidine (3) ,uridine (4) ,1-O-palmitoyl-3-d-galactosyl-sn-glycerol spongilipid (5) .Compound 5 exhibited weak cytotoxic activity with IC50 value of 10 .9 μmol/L .Conclusion Five compounds were obtained from a marine actinomycete Nocardiopsis sp .SCSIO 11492 .All five compounds were reported for the first time from this genus .Compound 5 could be the bioactive compound re-sponsible for the cytotoxic activity of Nocardiopsis sp .SCSIO 11492 .

Objective To study the expression of lipoic acid synthase(LIAS)in the liver and kidney of Leprdb/db mice with deficient leptin receptor. Methods Eight 10-week old male Leprdb/ +mice and Leprdb/dbmice were included in this study. The body weight of rats in the two groups was measured. Fasting blood glucose(FPG)was measured with blood glucose test strips for all mice after fasting for 8 hours. Blood samples were obtained from the abdominal aorta and the animals were sacrificed. The liver and kidney were weighed. The right lobe of liver and the left kidney samples were fixed in 4% paraformaldehyde for pathological examination. Serum samples were separated and the sereum contents of CHO, TG,HDL and LDL were detected. The mitochondria of liver and kidney tissues were extracted with a mitochondrial isolation kit, and the protein was extracted. The expression of LIAS protein was detected by western blot. Results Histopathological observation showed that the liver and kidney tissues of Leprdb/ +mice have intact and clear structure. But the liver tissue of Leprdb/dbmice showed fatty degeneration, the kidney tissue showed glomerular hypertrophy, basement membrane thickening, mesangial area widened, including mesangial cells and mesangial matrix increased. The GLU,CHO,TG,LDL and AST of Leprdb/dbmice were significantly increased compared with those of Leprdb/ +mice(P<0.05). Compared with Leprdb/ +mice,the LIAS protein expression was significantly increased in the liver and kidney mitochondria of Leprdb/dbmice(P<0.05). Conclusions There is impaired glucose and lipid metabolism in the Leprdb/dbmice which has defect leptin receptor,and the expression of LIAS protein in liver and kidney of the Leprdb/dbmice is higher than that of Leprdb/ +mice.

Objective To establish an efficient method of genotyping for Leprdb/ +mouse offsprings by TaqMan probe quantitative fluorescence PCR. Methods Genome DNA was extracted from tails of 228 Leprdb/ +mouse offsprings. PCR primers and TaqMan probes were designed according to the mutation sites of Lepr gene(rs1801133). Real time PCR assay was applied and SNP loci were typed with SDS software. The genotyping of 2-month old Leprdb/dbmice was validated by the phenotype and Hardy-Weinberg equilibrium test was performed. Results 228 samples were detected by the established TaqMan fluorescence quantitative PCR assay. 64 mice were of GG genotype, with a genotype frequency of 0.1929. 123 mice were of GT genotype, with a genotype frequency of 0.5395. 41 mice were of TT genotype, with a genotype frequency of 0.2807. Compared with the phenotype typing,the sensitivity of the TaqMan fluorescence quantitative PCR was 97.56% and the specificity was 99.47%. Conclusions TaqMan probe quantitative fluorescence PCR assay is a simple and efficient method,and can be used to detect the genotype of Leprdb/ +mouse offsprings.

Objective To understand the research hotspots and development trend of Hedyotidis Herba;To provide reference for related clinical studies.Methods The literature on Hedyotidis Herba was retrieved from CNKI,Wanfang Data,VIP and CBM from January 1,2003 to September 5,2023.NoteExpress 3.7 software was used to manage the bibliothems,and CiteSpace 6.2.R2 software was used to visualize the authors,institutions and keywords.Results Totally 2 713 articles were included,and the annual number of publications showed a fluctuating upward trend.There were 617 source journals,among which Shaanxi Journal of Traditional Chinese Medicine published the most articles(136).799 authors were involved,and the core authors included Liu Jian,Lin Jiumao,Peng Jun,etc.The main research institutions were Nanjing University of Chinese Medicine,Shandong University of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine and so on.In recent years,high-frequency keywords were experience of famous doctors,data mining,medication law,apoptosis,lung cancer,liver cancer,etc.The research frontier involved the mechanism,data mining and molecular docking.Conclusion The study of the Hedyotidis Herba mainly focuses on disease treatment,compatibility application,component analysis,mechanism,and the experience summary of clinical application of the Hedyotidis Herba.Data mining for medication law of famous doctors,prediction of potential drug targets through network pharmacology and molecular docking technology and exploration of their mechanisms are the research trends in this field.

BACKGROUND:In the initial stage of growth plate injury inflammation,platelet-derived growth factor BB promotes the repair of growth plate injury by promoting mesenchymal progenitor cell infiltration,chondrogenesis,osteogenic response,and regulating bone remodeling. OBJECTIVE:To elucidate the action mechanism of platelet-derived growth factor BB after growth plate injury. METHODS:PubMed,VIP,WanFang,and CNKI databases were used as the literature sources.The search terms were"growth plate injury,bone bridge,platelet-derived growth factor BB,repair"in English and Chinese.Finally,66 articles were screened for this review. RESULTS AND CONCLUSION:Growth plate injury experienced early inflammation,vascular reconstruction,fibroossification,structural remodeling and other pathological processes,accompanied by the crosstalk of chondrocytes,vascular endothelial cells,stem cells,osteoblasts,osteoclasts and other cells.Platelet-derived growth factor BB,as an important factor in the early inflammatory response of injury,regulates the injury repair process by mediating a variety of cellular inflammatory responses.Targeting the inflammatory stimulation mediated by platelet-derived growth factor BB may delay the bone bridge formation process by improving the functional activities of osteoclasts,osteoblasts,and chondrocytes,so as to achieve the injury repair of growth plate.Platelet-derived growth factor BB plays an important role in angiogenesis and bone repair tissue formation at the injured site of growth plate and intrachondral bone lengthening function of uninjured growth plate.Inhibition of the coupling effect between angiogenesis initiated by platelet-derived growth factor BB and intrachondral bone formation may achieve the repair of growth plate injury.

Bone marrow biopsy is one of the important means of hematopathological diagnosis, which has decisive diagnostic significance for various benign and malignant lymphohematopoietic system diseases. Its diagnostic value includes morphological observation, immunohistochemistry, genetics, and molecular biology testing. Owing to the unique nature of bone marrow biopsy, decalcification is an essential step in the pre-treatment process. Its purpose is to remove calcium from bone tissue, preserve intact collagen fiber components, facilitate tissue sectioning, and prevent tissue detachment during staining. If bone marrow biopsy lacks sufficient decalcification, preparing a section is difficult. Conversely, if decalcification is excessive, it can seriously disrupt tissue antigen activity. Therefore, a decalcification method with high decalcification efficiency and mild antigen damage is essential for bone marrow biopsy. This article introduces a bone marrow biopsy tissue decalcification method with high efficiency and less antigen loss: decalcification is performed at room temperature with 12% formic acid and 8% hydrochloric acid decalcification solution on a shaker.