Objective· To investigate whether vitamin E could reverse the disruptive effects of bisphenol A (BPA) on steroidogenesis and to explore the optimal vitamin E concentration.Methods· Rat primary granulosa cells were extracted and exposed to BPA (0,0.01,0.1,1,10,100 μmol/L).After 48 h of incubation,culture medium was collected and estradiol (E2) and progesterone (P4) were measured using ELISA kits.Then,granulosa cells were incubated with 5 μmol/L(average concentration in follicular fluid) or 25 μmol/L (high concentration in follicular fluid) vitamin E (α-tocopherol) or vitamin E (5 μmol/Lor 25 μmol/L) plus BPA (10 μmol/L or 100 μmol/L) for 48h,E2 and P4 were measured.Results· BPA at 10 μmol/L and 100 μmol/L suppressed E2 and P4 production in a dose-dependent manner (P＜0.05).Vitamin E at 25 μmol/L significantly increased E2 and P4 levels by (44.89±15.18) ％ and (43.33±8.82) ％(P＜0.05),respectively.Coincubation of the granulosa cells with BPA and vitamin E (5 μmol/L or 25 μmol/L) restored the productions of E2 and P4,which were not significantly different from the control group (P＞0.05).Conclusion· Vitamin E (5 μmol/L/25 μmol/L) could reverse BPA-induced reduction in steroid hormone production in rat ovary granulosa cells,and the antagonistic effect of 25 μmol/L vitamin E was more obvious than that of 5 μmol/L vitamin E.

Objective To study the methylation status of the promoter region of several tumor suppressor genes in p53-Bax mitochondrial apoptosis pathway and its role in cholangiocarcinoma. Methods The hypermethylation of the promoter region of tumor suppressors death-associated protein kinase (DAPK), p14, and target of methylation-associoted silencing-1 (TMS1/ASC) were detected by methylation-specific PCR. P53 gene status (exon 5-8 ) were examined by automated sequencing. The relationship between gene mutations and the biological behaviors of cholangiocarcinoma was analyzed. Results Methylation existed in at least one promoter region of tumor suppressor gene in the tumor tissues of 24 patients (66. 7% ). The frequencies of tumor suppressor gene methylation in cholangiocarcinoma were: p14 24%, DAPK 30. 6%, and TMS1/ASC 36. 1%. The frequencies of tumor suppressor gene methylation in the adjacent tissues were: TMS1/ASC 8.3% and DAPK 5.6%. DNA sequencing showed p53 gene mutation was found in 22 of 36 patients (61.1% ), and p53 gene mutation combined with the methylation of tumor suppressor was found in 14 (38.9%) patients, which was significantly correlated with pathologic biology, invasion, and differentiation ( P ＜ 0.05 ). The 1-year, 2-year, and 3-year survival rates were significantly higher in tumor-suppressing genes methylation group ( n = 4) (70%, 43 %, and 28%, respectively)than those in p53 gene mutation group (n = 14) (28%, 5%, and 0%, respectively) (χ2 =9. 060, P =0.03).Conclusions Promoter hypermethylation of p53-Bax mitochondrial apoptosis pathway is a common epigenetic event in cholangiocarcinoma. Although the methylations of TMS1/ASC and DAPK genes in the adjacent tissues are relatively low, they may be informative for the early detection of cholangiocarcinoma. P53 gene mutation combined with the methylation of tumor suppressor may be related with the pathologic biology of cholangiocarcinoma, making the latter trend to be with high malignancy and poor prognosis.

Objective To investigate effect of humanistic care model in patients with special threatened abortion.Methods A total of 36 hospitalized fetus reservation patients with special threatened abortion in our hospital from January to June 2016 was selected as control group,and 36 hospitalized patients in our hospital from July to December 2016 were as intervention group.The control group was given routine nursing,while the experimental group was additionally given humanistic care.Results There were no significant difference in scores of self-rating depression scale (SDS) and self-rating anxiety scale (SAS) score on admission (P ＞ 0.05),and the above indicators in two groups were decreased at discharge,and the intervention group was lower than the control group (P ＜ 0.05).The satisfaction of the experimental group was higher than that in the control group (P ＜ 0.05).Conclusion Humanistic care model can effectively relieve the depression and anxiety in patients with special threatened abortion and increase the patient's satisfaction.

Objective To investigate effect of humanistic care model in patients with special threatened abortion.Methods A total of 36 hospitalized fetus reservation patients with special threatened abortion in our hospital from January to June 2016 was selected as control group,and 36 hospitalized patients in our hospital from July to December 2016 were as intervention group.The control group was given routine nursing,while the experimental group was additionally given humanistic care.Results There were no significant difference in scores of self-rating depression scale (SDS) and self-rating anxiety scale (SAS) score on admission (P ＞ 0.05),and the above indicators in two groups were decreased at discharge,and the intervention group was lower than the control group (P ＜ 0.05).The satisfaction of the experimental group was higher than that in the control group (P ＜ 0.05).Conclusion Humanistic care model can effectively relieve the depression and anxiety in patients with special threatened abortion and increase the patient's satisfaction.