This study was aimed to establish a HPCE method for the content determination of hesperidin and phillyrin in Bao-He-W an (BHW). Fused silica capillary (75 cm í 50 μm) was employed and 30 mmol·L-1 borax so-lution (8% acetonitrile, pH9.64) was served as the running buffer. Other conditions were as follows: electrokinetic injection was 50 kPa í 20 s; analytical voltage was 20 kV; temperature was 20℃; and detection wavelength was 254 nm. The silica capillary was flushed with 0.1 mol·L-1 sodium hydrate and the running buffer for 10 min before each injection, respectively. The results showed that the linearity of hesperidin was in the range of 0.10~2.40 mg·mL-1 (r=0.999 4), the average recovery was 99.85% and RSD=2.34%. The phillyrin was in the range of 0.07~0.84 mg·mL-1 (r=0.999 2), the average recovery was 99.16% and RSD=2.78%. It was concluded that the method was rapid and sensitive. It can be used for the quality control of content determination of hesperidin and phillyrin in BHW.

Objective To study the metastasis rule and influence factors of cervical central group (Ⅵ region) lymph node in patients with papillary thyroid microcarcinoma.Methods The clinical data of 195 patients with papillary thyroid microcarcinoma were analyzed retrospectively.All patients received bilateral thyroidectomy and bilateral cervical central group lymph node dissection.Results All patients died without surgery and hospitalization,the incidence of postoperative complications was 2.1％ (4/195).The total incidence of cervical central group lymph node metastasis was 35.4％ (69/195).Factors relating to cervical central group lymph node metastasis rate were:age (OR =0.924),gender (OR =0.213),the number of foci (OR =2.208) and the sum of the tumor diameters (OR =2.106).Conclusions Patients with papillary thyroid microcarcinoma may suffer from lymph node metastasis of the cervical central group,and lymph node metastasis rate increases significantly in young or male patients,and when the lesions are multifocal or the sum of the tumor diameter ＞ 0.5 cm.

Research papers of TCM intervention in sub-health which published in biomedical journals from 1984 to 2008 were analyzed by bibliometrics methods in this article. We found that TCM intervention in sub-health attracted the concern of the researchers increasingly; Fund mainly came from nation and provincial government departments; TCM hospital,TCM research institutes and TCM universities had played a leading role in studying TCM intervention in sub-health; liver depression and spleen deficiency syndrome, Yin deficiency of liver and kidney syndrome and deficiency of both heart and spleen syndrome were three common syndromes in sub-health; herbs and modified prescription were the main forms of Chinese medicine intervention in sub-health. In addition, we analyzed the frequency of herbs and acuppoints appearing in literature, and discussed relevant thinking of TCM clinical intervention.

0.05).At T9,the Ppeak and PETCO2 in LMA group were significantly higher than those in ET group [(19.0?5.0) mm Hg and(44.7?3.1) mm Hg vs.(13.0?3.0) mm Hg and(34.1?1.9) mm Hg respectively,t=5.636,P=0.000 and t=15.968,P=0.000].In LMA group,5 patients developed adverse reaction,while in the ET group,13 patients showed the reaction(?2=3.774,P=0.052).Conclusions Ventilation with LMA is safe and reliable for elderly patients undergoing laparoscopic surgery with a low rate of adverse reaction.It has slight effect on the hemodynamics of the patients.

Objective To compare the clinical efficacy between single span video-assisted thoracoscope (VATS) surgery and traditional VATS surgery for spontaneous pneumothorax in elderly patients.Methods A total of 88 elderly patients diagnosed as spontaneous pneumothorax by chest X-ray and CT were selected and randomly divided into single span VATS group and traditional VATS group (n=44,each).The bleeding volume,duration of operation,hospitalization time,time of postoperative analgesic use,chest tube indwelling time,postoperative complications and adverse reactions were observed.All patients were follow-up for 1-2 years to record the recurrence rate and death rate.Results All patients were received VATS surgery successfully.Their clinical symptoms disappeared and X-ray showed that pneumothorax disappeared.There were significant differences in bleeding volume,time of postoperative analgesic use,hospitalization time between single span VATS group and traditional VATS group [(46.7±18.8)ml vs.(46.7±18.8)ml,(1.7±0.9)d vs.(2.4± 1.7)d,(6.3±1.7)d vs.(9.1±2.1)d,t=3.04,2.18,5.59,respectively,all P＜0.05].The duration of operation and chest tube indwelling were shorter in single span VATS group than in traditional VATS group (both P＜0.05).Patients with chest pain and incision numb after operation were less in single span VATS group than in traditional VATS group (11 cases vs.26 cases,7 cases vs.21 cases,t=10.50,10.26,both P＜0.05).There were no significant differences in recurrence rate and death rate between the two groups.Conclusions Single span VATS surgery is an safe and effective treatment for spontaneous pneumothorax in elderly patients,which has a better postoperative recovery and fewer adverse reactions.

Objective To sum up the experiences of diagnosis,treatment and medical evaluation of the anatomical abnormalities in nasal cavity(AANC,including deviation of nasal septum and turbinate hypertrophy) in aircrew.Methods Case data of 91 pilots with CANC were analyzed.The classification and typing of AANC and secondary barotraumas associated with typing of AANC were studied.Results There were 50 cases of class I AANC(with symptoms or signs on ground),41 cases of class II(no symptoms or signs on ground but with symptoms of secondary barotraumas in flight,including IIa 10 cases:with symptoms of secondary barosinusitis,IIb 26 cases:with symptoms of secondary barotrauma of ear,IIc 5 cases:with symptoms of secondary barosinusitis and barotrauma of ear after flying).For the typing of AANC,there were 41 cases of nasal septum deviation,29 cases of inferior turbinate hypertrophy,8 cases of middle turbinate hypertrophy,and 13 cases of nasal septum deviation and turbinate hypertrophy.85 cases were still in a qualified position to continue their flying job,1 pilot was transferred from fighter to transport pilot,2 pilots were temporarily grounded,and 3 were permanently grounded.Conclusions AANC is very common in aircrew.AANC in aircrew is classified and typed according to the effect of gas pressure adjustment functions of ear and nasal sinus.The classification and typing are of important implication in clinical aviation medicine.Medical workers must unify the qualification criteria in selection for air candidates,and also in diagnosis,treatment,medical evaluation of disease in aircrew.This is an important measure to improve the health quality and ensure combat effectiveness for the air force.

Objective:To evaluate the therapeutical effects of diplopore puncture of tympanum and intratympanic administration(DPTIA) on patients with acute secretory otitis media(ASOM).Method:One hundred and forty -one cases with ASOM were divided into two groups randomly,with 69 cases(80 ears) in group A(experimental group) and 72 cases(84 ears) in group B(control group).Group A was treated with DPTIA combined with medication, group B was treated with haplopore puncture of tympanum combined with medication. All patients accepted 1 to 3 courses of treament,and were observed during the period of treatment and 3 months after treatment.Result:The factors (including gender, age, side of ailing ear, course of disease, air-bone gap in the pure tone average,and acoustic immitance type in ailing ear) which may impact the prognosis were matched in two groups prior treatment(P＞0.05). While there was a significant difference in the cure rate and the total effective rate respectively between two groups after treatment(P＜0.05).No adverse reaction or complication was seen.Conclusion:DPTIA is a safe, reliable and effective way to manage patients with ASOM.

Ethnic medicine is an important component of traditional Chinese medicine and excellent traditional culture. New products of ethnic medicine are important application form to play therapeutic advantages of ethnic medicine, whose research and development should focus on the maintenance of characteristics of ethnic medicine. From the perspective of the research and development of ethnic medicine, this article discussed research ideas in the development of new products of ethnic medicine and put forward that attention should be paid to the experience on using the ethnic medicine, related literature studies, and ethnic medicine resource protection and utilization as well. In addition, this article put forward the research tactics in new products of ethnic medicine, such as medicinal materials, effective parts, active ingredients, the secondary development research and so on, with a purpose of improving the level of researching and developing new products of ethnic medicine.

Thirty-seven cases of brain glioma,confirmed by light microscopy and immuno-'histochemistry were studied with transmission electron microscopy.It was found that there were certain ultrastructural features for each type and each grade of glioma.In astrocytoma,there was certain amount of glial filaments in the tumor cells; Rosenthal fibers consisted of irregular osmiophilic masses surrounded with glial filaments,occasionally cytoplasmic annulate lamellae and intranuclear filaments could be seen,and the interstitial capillaries were characteristic.In typical oligodendroglioma,astrocytic processes containing glial filaments were quite present in different amounts.These findings suggest that observation on the ultrastructure of gliomas is of significance to establish the diagnosis,to assess the degree of differentiation,and to identify some rare structures which can reveal the essence of the tumor.In addition,ultrastructural observation is helpful for prognosis.

B ased on Chinese medicine pharmacological literature data , the property , flavor , toxicity , meridian tropism, efficacy, and clinical application of Chinese herbal medicine were used as a set of attributes. The sup-port vector machine ( SVM ) was used in the establishment of predictive models of 187 pharmacological effects of Chinese herbal medicine respectively. And the cross-validation method was used to determine the accuracy of predictive models . After that , the predictive models with the predictive accuracy rate greater than 90% were used to predicate pharmacological effects of 624 herbals recorded in the Chinese Pharmacopoeia(2010 edition). It was found that the accuracy rate of 108 models was greater than 90%, and the accuracy rate of antibacterial effect predictive model was 99.76%. The highest predictive value of Chinese herbal medicine was the anti-oxi-dation effect of Menispermi Rhizoma.