AIM:To estimate the toxicological characterization of Jiutong Capsule(Radix Puerariae lobatae,Fructus seu Semen Hoveniae,etc.).METHODS:The acute toxicity test,micronucleus test of bone marrow cell,sperm shape abnormality test in mice,the Ames test,and 30 days feeding test in rat were performed.RESULTS:(1) The acute toxicity test showed that LD_ 50 of Jiutong Capsule was more than 10.0 g/kg.(2) The result of Ames test,micronucleus test of bone marrow cell,and sperm shape abnormality test were negative.(3) The 30 days feeding test showed that Jiutong Capsule had no cumulate toxicity in mice.CONCLUSION:The results show that Jiutong Capsule does not have toxicity,and it can't cause mutation and heredity toxicity.

Cartilage antuumor component (CATC) was isolated from a 1 mol/L guanidine hydrochloride extract of bovine cartilage by acetone fractioned precipitation and superfiltration. Using human skin fibroblasts as a normal control, it was demonstrated that CATC inhibited the DNA synthesis of Hela, QGY7703 tumor cell lines and bovine artery endothelial cells, but accelerated the normal cells, when the concentration was below 1250 ?g/ml. At the concentration of 5 000 ?g/ml, CATC inhibited the two cell lines. With human tumor stem cell assay, CATC inhibited the stem cell growth of Hela and QGY7703 cell lines. These suggest that CATC has the effects of inhibiting angiogenesis and tumor cells.

This article was aimed to survey the use of Chinese minority medicine, in order to provide differences and similarities among different Chinese minority medicine, and to analyze the relations among species resources, using range, functions and indications. It provided a basic scientific platform for the development, research and use of minority medicine. The books and journals published since the late 1970s (the country after the reform and opening up) had been collected, classified. And reference books on current Chinese minority medicine had been compiled. The results showed that the first draft of the book had been completed and delivered to the press. The dictionary had cited 53 traditional medicine used by minority groups. The total number of minority medicine was 7 734. The total characters amount in the book was 1 700 000 words. It was concluded that the species of minority medicine were various. There was rich information on their harvest, medicinal parts, functions and indications with prominent features. The existing major problems were to clarify the standard of the species as well as their major functions and indications.

Objective To investigate the impact of cluster nursing intervention on the patients with ventilator-associat-ed pneumonia in ICU. Methods From January 2012 to January 2014,selected 100 cases associated ventilator pneumo-nia patients in our hospital ICU,according to the different caring methods were randomly divided into study group and control group,the control group received usual care,the study group focused cluster of nursing intervention,the expec-torant effect were compared between two groups,the mechanical ventilation and ICU stay were compared. Results The expectorant effect of study group was better than the control, expectorant total effective rate of the study group was 94%,compared with the control group, the difference was statistically significant (P<0.05). Duration of mechanical ventilation of patients in study group was significantly shorter than the control group(P<0.05). The average ICU stay of study group was significantly shorter than the control group between than the two groups,the difference was statistical-ly significant (P<0.05). Conclusion The implementation of the cluster of nursing interventions help to improve patient outcomes ICU ventilator-associated pneumonia,shorter hospital stay,worthy of promotion and application.

Objective To investigate the impact of health education intervention on complications and quality of life in patients with coronary heart disease interventional treatment. Methods A total of 68 patients from January 2011 to January 2014 in our hospital ICU hospitalized with coronary heart disease interventional were selected and divided into intervention and control group, 34 cases each, after implementation symptomatic care, the intervention group focused on health education intervention, the control group had no health education intervention system, the incidence of postoper-ative complications and quality of life of patients and hospital SAQ scores were compared between two groups. Results The rate of postoperative complications in the intervention group was 8.8%, was significantly lower than the control group (P<0.05). At discharge, the patient's quality of life in the intervention group SAQ scores in physical activity were limited extent, the stability of angina pectoris, angina frequency, treatment of subjective feelings of satisfaction and disease more significant changed compared with preoperative relatively, and the changes in the intervention group were more significantly than the control group (P<0.05). Conclusion The implementation of the system for percutaneous coronary intervention health education interventions for patients can helps to reduce the incidence of postoperative complications and improve quality of life in patients with coronary intervention, worthy of promotion and application.

In China, the treatment of AIDS With Chinese herbal medicine compound has been widely studied and practiced. Clinical Studies showed that the treatment of AIDS with Chinese medicine can significantly relieve AIDS patients' clinical symptoms, improve life quality with rising immunity and alleviate toxic and side effects in antiviral drug. This study took 28 Clinical Studies on the treatment of AIDS with Chinese herbal medicine compound published in Chinese periodicals as the research object, and designed Tree Analysis Algorithm was used to analyze prescription compatibility in the treatment of AIDS with Chinese herbal medicine compound. Results of data analysis revealed that clinical therapy on AIDS could be classified into three types: predominent de-toxication, predominent supplementing Qi, and simultaneous de-toxication and supplementing Qi due to different understanding the pathogenesis of excessive evil and deficient essence-qi. But in practical medication, there has not formed a reasonable and effective combination or basic prescription up to now, except that Astragalus was wildly used.

Objective: To identify underlying reasons for discrepant cases of positive cytology but negative histology. Methods: Cases with positive cytology and negative histology from 2008 to 2016 were retrieved from Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University. Low grade squamous intraepithelial lesion or higher grade lesions were considered as positive cytology test in the study. Consecutive follow-up biopsies and as well as sites of biopsy were documented for analysis. Results: Overall positive rate of biopsy followed positive cytology was 74.3%(8 990/12 097). Of the negative biopsies, 675 cases were followed-up with multiple biopsy. Two-hundred and eighty-seven cases (42.5%, 287/675) were confirmed to have lesions. Comparing with those with initial positive biopsiews, patients of the latter group were significantly older and had other specimen types including vaginal biopsy, cone biopsy and hysterectomy. The final histological diagnoses were well correlated with cytological results (Kappa=0.505, P<0.01). Conclusions Qualified cervical cytology is complimentary to histological diagnosis. Clinicians should not ignore the positive cytological result prior to a normal histological diagnosis. In contradictory cases, repeated colposcopy and biopsy at extended anatomic sites may reveal additional lesions.

Aim To explore the possibility of the multiple epitope DNA vaccines of hepatitis C virus (HCV). Methods A synthetic multiple epitope antigen gene PCX of HCV was cloned into vector pREP9(RSV promoter) and pcDNA3 (CMV promoter) to construct eukaryotic expression vectors pREP9/PCX and pcDNA3/PCX, then they were used to immunize mice and rabbits, the titer of specific humoral and cellular responses were detected and their safety were observed. Results In mice, specific anti-GZ-PCX antibody(IgG) was lower than 1∶ 1 000 and did not persist well. In rabbits, the highest titer of anti-GZ-PCX IgG reached at 1∶ 3 200 and remained for about one month. Delayed type hypersensitivity reactions (DTH)and proliferation response of peripheral lymphocytes were induced by GZ-PCX antigen. Body weights of immunized mice were normal and no obvious toxic reaction was observed. Conclusion The multiple epitope antigen gene of HCV could induce specific immune responses without obvious toxicity and it might be able to serve as an effective HCV vaccine candidate.

Objective To observe the clinical curative effect of locking plate and anatomical plate for the treatment of complex tibial plateau fractures. Methods Retrospective analyzed clinical data of 80 cases of complex tibial plateau treated in our hospital from January 2013 to January 2014, according to the different internal fixation methods and di-vided into observation group (n=40, locking plate fixation) and control group (n=40, normal anatomical steel plate treatment), through the review and analysis home visits, telephone follow-up, and review records of two groups were compared, the operation time, blood loss, operation group and control group in the amount, full weight bearing time, HSS score, complications, and the healing time of fracture by X-ray and clinical examination judgment were compared. Results The average operation time of observation group was(112.3±16.1)min, intraoperative blood loss(285.9±21.8)mL, the average operation time of control group was(130.8±22.3)min, blood loss(321.6±37.9)mL. All fractures were healed, the healing time of observation group was(13.0±1.2) weeks, full weight bearing time (14.1±1.7) weeks, the control group of fracture healing time (15.4±2.7)weeks, full weight bearing time was(16.5±2.9) weeks; HSS score of the ob-servation group was (88.3±7.2)points, the control group was (83.1±6.3)points. Comparison of two groups of patients with the above indexes, with significant difference (P<0.05 or P<0.01), the excellent rate 1 year after the operation of observation group was 90.0%, significantly higher than that in control group, the excellent and good rate was 80.0%, excellent rate of two groups with knee joint function, the difference was significant (P<0.05); the complication rate of observation group was 7.5%, the control group was 22.5%, with significant difference (P<0.05). Conclusion Compared with normal anatomical plate, the complex tibial plateau fracture locking has less bleeding, fast fracture healing, less complications and other advantages of steel plate, and can significantly improve the knee joint function of patients, is worthy of popularization and application.