Objective To explore the influence of occupational stress and personality traits on job burnout of nurses from psychiatry-specialized hospitals and general hospitals in Wenzhou .Methods 110 nurses from psychiatry-specialized hospitals and 103 nurses from general hospitals in Wenzhou were surveyed by questionnaires of Nurses′Occupational Pressure Source Scale , Eysenck Personality Questionnaire (EPQ), and Maslach Burnout Inventory (MBI).Independent-Samples t Test was used for comparison of occupational stress , personality traits and job burnout of the two groups , and multiple regression analysis was used to analyze the data.Results Total average scores of occupational stress of nurses in psychiatry-specialized hospitals were (2.57 ±0.37), higher than the those of nurses in general hospitals (2.19 ±0.42), (t=2.158, P<0.05). Scores of the dimension of emotional stability in EPQ of nurses in psychiatry-specialized hospitals were (54.68 ± 9.04), higher than those of nurses in general hospitals (45.16 ±10.39), (t=2.184, P<0.05).For nurses in psychiatry-specialized hospitals, scores of emotional exhaustion in MBI were (28.40 ±5.50), and scores of depersonalization were (10.59 ±3.31), higher than those of nurses in general hospitals; while scores in dimension of low sense of achievement were (22.16 ±3.45), lower than those of nurses in general hospitals (t=2.381, 2.330, -7.694; P<0.05).It was revealed in multiple regression analysis that for nurses in psychiatry-specialized hospitals , emotional stability had a positive predictive function on emotional exhaustion ;working environment , resources and emotional stability had a positive predictive function on depersonalization ;nursing profession and work had a negative predictive function on low sense of achievement ;for nurses in general hospitals, patient-caring had a positive predictive function on emotional exhaustion;workload and time allocation had a positive predictive function on depersonalization; patient-caring, emotional stability, workload and time allocation had a negative predictive function on low sense of achievement .Conclusions Degree of job burnout of nurses in psychiatry-specialized hospitals is higher than that of general hospital nurses .The influences of occupational stress and personality traits on job burnout have a synergistic effect .