Objective The adsorption effects of four sorts of dietary fibres from seaweeds on exogenous NO~-_(2) were studied in vitro and in vivo.Methods By simulating the environment of stomach and small intestine in vitro,and feeding rat in vivo.At the same time,the adsorption effect of wheat bran was studied comparatively.Results The results showed that:(1) the adsorption effect of dietary fibres on NO~-_(2) were related with the pH and the species of dietary fibres.Under the simulated stomach environment,the adsorption capacity of Laminaria japonica was the strongest with 1.19 mg?g~(-1),the next was Eucheuma with 0.94 mg?g~(-1),and Sargassum was the weakest with 0.60 mg?g~(-1).The adsorption ratio of Laminaria japonica and Eucheuma was significantly higher than that of wheat bran(P

BACKGROUND: Heme oxygenase-1 (HO-1) plays an important role on preventing tissues and organs from oxidant stress injury, which remains currently one of the most active areas of investigation. OBJECTIVE: To study HO-1 over-expression on the survival and fiver function of rats after reduced-size liver transplantation by constructing recombinant adenovims Ad5-HO-1.DESIGN, TIME AND SETTING: Randomized controlled animal study was performed in the Institute of Pediatrics, Children Hospital Affiliated to Chongqing Medical University from September 2003 to March 2005. MATERIALS: Seventy-four SD rats were used to establish in situ reduced-sized liver transplantation models. METHODS: Recombinant adenoviral vector encoding Ha-1 gene (AdS-HO-1) was generatred by molecular biology method, wluch was administered to donors via voln at 48 hours before transplantation. All rats were randomly divided into control group(n=12),saline group(n=12), cobalt protoporphyrin (CoPP)group(n=13),Ad5-HO-1 group(n=13),Ad5-green fluorescent protein(GFP)group(n=12),and Ad5-HO-1+zinc protoporphyrin (ZnPP)group (n=12).Livers of donor wefe harvested and stored for 24 hours at 4℃ in HTK solution. Before it is implantedinto recipient. MA IN OUTCOME MEASURES: Survival rate and liver function; portal vein blood flow monitored 2 hours after transplantation by Color Doppler How Imaging; pathological changes of transplanted liver tissue observed by HE staining;HO-1 activity, tumor necrosis factor-alpha(TNF-α),Bcl-2 and Bax expression detected by immunohistoehemical staining; changes of HO-1,TNF-α,bcl-2 and bax mRNA detected by molecular viewpoint. RESULTS: Survival rate in the Ad5-HO-1 group was significantly higher than that in the saline group at al,7,and 21 days after liver transplantation(P＜0.05).Glutamic pyruvic transaminase decreased in the Ad5-HO-1 group as compared to that in the saline,Ad5-GFP,and Ad5-HO-1+ZnPP groups(P＜0.05);portal venous blood flow significantly increased 2 hours after transplantation(P＜0.05);HO-1 activity also significantly increased(P＜0.05).RT-PCR and immunohistochemical staining showed that HO-1 and bcl-2 expressions increased(P＜0.05),but bax and TNF-αexpressions decreased(P＜0.05).CONCLUSION:Ad5-HO-1 significantly induces high expression of HO-1 increases portal venous blood flow within 2 hours alter liver transplantation, and promotes liver functional recovery so as to prolong survival time of rats after liver transplantion.

Objective To observe the effects when treating craniocerebral injuries with hyperbaric oxygen. Method 61 cases of craniocerebral injuries were treated with HBO. Result Cured: 34 cases (55.7% ), excellent effects: 17cases (27.8% ), effective 7cases (11.5% ), non-effective 3cases (4.9% ), the effective rate was 95.1% . Conclusion HBO is effective on craniocerebral injuries and it is the main aiding treating method of craniocerebral injury rehabilitation treatment.

Objective To study the feature of neonatal infections and characteristics of antibiotic treatment in a tertiary children ' s hospital. Methods Clinical data including incidence of infection, primary disease,species of bacteria, complication and antibiotic utilization in hospitalized patients from Jan. 2010 to Dec. 2012 were retrospectively reviewed using their medical records. Results Among 1826 patients admitted to neonetal surgery ward, 542 infants ( 29. 7％) were with infection. The incidence of antibiotic resistance was 23. 51％. The top five infectious diseases were:perianal abscess, necrotizing enterocolitis, colicitis, omphalitis and subcutaneous gangrene. 12 cases of multi-resistant infection were cured by non-restricted antibiotics. 109 were cured by restricted antibiotics. And other 7 were cured by special antibiotics. No death nor multi-resistant nosocomial infection were found. Risk factors including multi-site infection, premature or low birth weight infants, liver, kidney or heart dysfunction,fever lasting more than 3 days after antibiotic therapy, septic shock, sepsis, digestive tract perforation and peritonitis,were vital in choosing specific antibiotics. Conclusions Infection is one of the most common diseases in neonatal surgery ward, with major pathogens sensitive to antibiotics. The clinical characteristics and drug sensitive test are conductive to the reasonable use of antibiotics. Special antibiotics can be used directly in patients with risk factors Clinical doses of antibiotics in neonates depend on the monitoring of drug concentration.

OBJECTIVE: To investigate the influential factors associated with the long-term effect of allergic rhinitis (AR) specific immunotherapy (SIT). METHOD: Retrospective analyzed the following visual analog scale(VAS) before and after the specific immunotherapy of 219 patients with allergic rhinitis. Logistic regression and pair t-test were used to explore the key factors influencing the prognosis. RESULT: (1) The gender and the VAS before specific immunotherapy (P < 0.05) were associated with the long-term effect of allergic rhinitis specific immunotherapy, while family history, the age of specific immunotherapy, number of allergens and stitches and with other allergic diseases (P > 0.05) were not associated with the long-term effect of specific immunotherapy; (2) Compared VAS of different endpoints with VAS before the treatment,there were significant differences. The primary endpoints were the time following no more than half a year (total effective rate: 50.0%), half a year to 1 year (total effective rate: 51.7%), 1 to 2 year (total effective rate: 47.1%), 2 to 3 year (total effective rate: 54.5%) and over 3 year (total effective rate: 40.7%). CONCLUSION Apart from the gender and the VAS before specific immunotherapy,genetic and other factors can not significant influence the long-term effect of specific immunotherapy. The effect of specific im- munotherapy seems to last for 3 years at least. Increasing stitches can not improve long-term effect of SIT,but it can consolidate the effect. The long-term effect of specific immunotherapy tends to drop off with fluctuations over time and the higher of VAS before SIT,the better effect of long-term SIT. Further more, the long-term effect of SIT of male is worse than female. In consequences, we speculate epigenetics related to the interaction between environmental factors and genetic factors plays an important role in the long-term effect of allergic rhinitis specific immunotherapy.
Allergens ; Female ; Humans ; Hypersensitivity ; Immunotherapy ; Male ; Retrospective Studies ; Rhinitis, Allergic ; therapy ; Sex Factors ; Time Factors

Objective To review the diagnosis and treatment of pyloric canal polyp in infants and toddlers suffering progressive gastric outlet obstruction.MethodsClinical data of 5 male infants and toddlers,at age of 10 to 26 months(mean 15.6 months)with pyloric canal polyp admitted in our department from January 1993 to February 2009 were analyzed.ResultsOf 5 cases,the starting was acute and severe.the conservative treatment was out of effect and no any assistant examination was helpful during diagnosis,and every case was diagnosed clearly through exploratory laparotomy,cured with polypectomy and classic pyloroplasty.ConclusionCurrently,the exploratory laparotomy is an important way to diagnose pyloric canal polyp in infants and toddlers,while other assistant examination seems useless in this aspect.Polypectomy and classic pyloroplasty should be carried out during exploration,and it is dangerous for their life without correct therapy in time.

Objective To evaluate the effects of the short-term intervention lifestyle intervention on metabolic measurements of community patients with impaired glucose regulation (IGR). Methods A total of 90 IGR participants were randomly assigned to the control group (n=45) or the intervention group (n= 45). The subjects in the control group received routine diet and physical exercise advice once a month. The subjects in the intervention group received additional individualized diet counseling and circuit-type resistance training. Metabolic parameters were compared before or after the intervention between the two groups. Results In oral glucose tolerance test (OGTT),2-h plasma glucose (PG) and homeostasis model of insulin resistance index (HOMA-IR) were significantly decreased in the intervention group at 3 months(F= 13.47 or 82.25 ,both P < 0.05). Body mass index (t=-2.44, P<0.05), systolic blood pressure (t= -3.39, P<0.05), diastolic blood pressure (t=-3.97, P<0.05), fasting plasma glucose (t=-3.89, P<0.05),2-h PG (t=-7.22,P <0.05) ,total cholesterol (t=-2.72,P<0.05),low-density lipoprotein cholesterol (t=-2.74, P<0.05), and glycosylated hemoglobin A1 C (t=-3.73, P<0.05) were significantly declined in the intervention group compared to the control group (all P<0.05). Conclusions Intensive lifestyle intervention can significantly improve the metabolic markers of IGR subjects and should be used to prevent type 2 diabetes.

Objective:The nutritional value and edible safety were evaluated for fastfood Eucheuma. Methods:The nutritional composition and the contents of heavy metal element and microbiology index were tested, and the test of acute toxicity and mutation were performed to evaluate the nutritional value and edible safety. Results:The main components of fastfood Eucheuma were dietary fibre and mineral components, and the contents of protein and fat were very low. The gross content of dietary fibre was 88.6%. The contents of Zn and Ca were the highest among the mineral components.The index of heavy metal elements and microbes(cfu) were lower than that of the national standard. It was non-toxic and non-mutagenic. Conclusion:The fastfood Eucheuma is a functional food, and also a safe food of new resource.

Objective:To observe the killing effect of irradiated peripheral blood mononuclear cells (PBMCs) at low dose combined with apogossypolone (ApoG2) on cultured human prostate cancer PC-3 cells.Methods:Human PBMCs were irradated by gamma ray at 1 gray,the irradiation dose rate was 17 Gy/min.The experiment were divided into PC-3 tumor cell control group,PC-3 cells with irradiated and non-irradiated PBMCs co-culture groups,ApoG2 treatment group,irradiated PBMCs and ApoG2 co-treatment group.Acridine orange/ethidium bromide (AO/EB) staining and MTT method were used to observe the killing effect of PBMCs and/or ApoG2.Results:The killing activity of irradiated PBMCs group and ApoG2 treatment group were obviously increased and were higlaer than that of non-irradiated group (P＜0.05).The killing activity of combined group were much higher than that of irradiated group and ApoG2 treatment group (P ＜0.01 ).Conclusion:Irradiated PBMCs at low dose combined with ApoG2 can enhances the anti-tumor effects markedly.

Objective Adsorption effect of dietary fibers from seaweeds on extrinsic estrogens was studied.Methods The methods included the tests in vivo and in vitro which simulated environments of stomach and small intestine of rat. Results The adsorption effect of four kinds of dietary fibers on estradiol in simulated environments of small intestine was better than that in simulated environments of stomach. The effect of Laminaria japonica was the best. And the rate of adsorption on estradiol was 108 g?g~ -1.It is 1.5,1.8, 3.1, and 3.0 times that of Sargassum,wheat blan,Gracilaria and Eucheuma,respectively. The rates of adsorption on estradiol of four kinds of dietary fibers from Gracilaria, Eucheuma, Sargassum and laminaria japonica was 11.1%,13.6%,27.5% and 38.7%, respectively. Adsorption rate of Sargassum and laminaria japonica was 1.9 and 1.3 times than that of wheat bran, respectiveiy which was 20.4%. While for Gracilaria and Eucheuma, adsorption rate was lower than that of wheat bran. Conclusion The dietary fibers from four seaweeds showed adsorption effect on extrinsic estrogens. The dietary fibers from brown seaweeds (Sargassum and laminaria japonica) had more adsorption effect than that of red seaweeds (Gracilaria and Eucheuma).