The pathogenesis of gestational diabetes mellitus (GDM) remains unclear. Recent studies have found that a dysbiosis of gut flora could lead to metabolism and immune system disorders in human beings, resulting in GDM. The gut microbiome changes can be affected by various factors, of which diet and antibiotic exposure are the most dominant. From the perspective of the gut microbiome changes, individualized dietary guidance, evidence-based administration of antibiotics, appropriate intake of prebiotics, and probiotics supplement may help prevent and treat GDM.

Objective To investigate the role of klotho protein in the pathogenesis of hypertension. Methods We collected blood samples of 104 patients with hypertension and 61 people without hypertension admitted to our hospital between March and June 2009. Klotho protein and nitric oxide (NO) in blood serum were detected by ELISA and nitro-reductase methods. Results Klotho protein absorbance and NO were lower in hypertension group than in non-hypertension group (P

Objective To observe the value of serum cardiac troponin Ⅰ(cTn-Ⅰ) on the diagnosis of acute myocardial infarction (AMI). Methods The serum cTn-Ⅰand CK-MB of 30 elderly patients(≥65 y) and 30 middle-aged patients(

Objective To investigat the relationship between PON_1 192 Gln-Arg gene polymorphisms and coronary artery lesions in Shaanxi Han Chinese.Methods A total of 222 cases with one or more than two branches narrow(≥50%) and 164 cases with no coronary artery narrow(0.05).Conclusion There was no relationship between PON_(1) 192 Gln-Arg gene polymorphisms and Shaanxi Han patients with coronary heart disease.

Objective To assess the protective effect of the proteasome inhibitor bortezomib on rat liver subjected to bile duct obstruction. Methods Thirty rats were divided randomly into three groups, which were sham-operation group (SO group), bile duct ligation control group (Con group) and bortezomib group (Bor group). All rats in the Con group underwent ligation of the common bile duct, and rats in the Bor group were given bortezomib intrabominally at-1 d, 4 d post-ligation of the common bile duct. All the rats were sacrificed at 7 d post-surgery. ALT, TB and TBA levels were determined. The expression of NF-κB p65 was assessed using immunohistological staining. RT-PCR was employed to detect TNF-α mRNA levels in liver samples. Results There was no significance in the levels of TB and TBA between Con and Bor groups. The ALT revel in the Bor group [(92.4±21.4)μmol/L]was significantly lower than that in the Con group [(145.7 ±33.5) μmol/L], P＜0.05. The positive staining rate of NF-κB p65 subunit in the Bor group showed significant lower value (11.6% ±2.7 % ) compared to that in the Con group (15.5 %±4.3 % ), P＜0.05. The expression ratio of TNF-α mRNA in the Bor group was 1.0± 0. 2, which also significantly lower than that in the Con group (1.3±0.4), P＜0. 05. Conclusion These data suggest that the proteasome inhibitor bortezomib reduces rat hepatocyte injury in the bile duct ligation by mechanisms associated with the inhibition of NF-κB as well as the attenuation of inflammation.

Objective To investigate the diagnostic value of serum Cystatin C for early detection of renal damage in patients with liver cirrhosis. Methods 24-h creatinine clearance (CCr), serum level of cystatin C (CysC) and serum creatinine were measured in 76 patients with cirrhosis and t-test,Pearson's correlation test and ROC curve were used to evaluate the diagnostic significance of Cys C.Results The increase in Cys C level was associated with a decrease of CCr in the patients. Both Cys C and SCr were inversely correlated with CCr ( CysC: r =- 0. 763, P ＜ 0. 001; SCr: r=-0. 571,P＜0.01). Meanwhile, the area under the ROC curve was significantly higher in Cys C than in SCr (0. 830 vs. 0. 612). Conclusion Cystatin C is a more accurate and sensitive marker of renal diagnosis in liver cirrhosis. Detection of Cys C level in cirrhotic patients is of great significance for the prevention of liver-kidney syndrome.

Objective To determine the expression of CD4+ CD25+ FOXP3+ regulatory T cells(Treg cells) in peripheral blood of patients with hepatocellular carcinoma (HCC) and investigate the clinical significance of Treg cells determination in clinical practice. Methods Flow cytometry was employed to measure the levels of CD4+ CD25+ T cells and CD4+ CD25+ FOXP3+ T cells in peripheral blood of 18 HCC patients, 26 hospitalized patients without HCC (clinical control) as well as 24 healthy persons (healthy control). Results The percentage of CD4+ CD25+ T cells in total CD4+T cells isolated from the HCC patients(4.25% ± 3.98 % ) was elevated significantly compared to that in the clinical control group (1.34% ± 1.14%) or healthy control group (1.29% ±0.95%) (both P＜0.01). There was no difference in the percentage between the clinical control group and the healthy control group (P＞0.05). Meanwhile, the ratio between CD4+ CD25+ FOXP3+ T cells and CD4+ T cells in HCC patients (2.94%0.91%) also increased significantly compared to that in the clinical control group (0.76% ± 0.34%) or healthy control group (0.81% ± 0.29%) ( both P＜0. 001), which showed a more obvious increasing tendency than the ratio of CD4+ CD25+ T cells and CD4+ T cells. No difference in percentage of CD4+ CD25+ FOXP3+ T cells in CD4+ T cells was found between the clinical control group and the healthy control group (P＞0.05). Conclusion As the more accurate regulatory T cells, CD4+ CD25+ FOXP3+ T cells are able to detect the increase of population in HCC patients. Therefore, it is important to determine the levels of CD4+ CD25+FOXP3+ T cells in HCC patients for prevention and treatment of malignancy.

Objective To study the protective effect of Trimetazidine on myocardial injury in mice.Methods 6 to 7 week-old male BALB/c mice were randomly divided into 3 groups at twelve for each group:normal saline (NS) group,ISO group and TMZ group,N.S group for blank control group,ISO group for damage group,TMZ as treatment group.After 4 weeks,cardiac index (HW/BW) was calculated,serum lactate dehydrogenase (LDH) activity were measured by enzyme standard method.Malondialdehyde(MDA) was detected by thiobarbituric acid method(TBA).Superoxide dismutase (SOD) activity were determined by WST-1 method.The concentrations of serum Klotho were measured by ELSIA.The cardiac muscular tissue was imbedded with paraffin and colored with HE,Massion and caspase-3.Results The experiment model meets the requirements.In TMZ groups' HW/BW,LDH activity and MDA level were significantly lower than those of the ISO group(P ＜ 0.01).SOD energy,concentration of Klotho was significantly higher than those of the ISO group(P ＜ 0.01).HE color results showed that the myocardial cell diameter of ISO group was obviously larger than that of NS group,combined with disordered muscle fibers and hyperplastic myocardial interstitial fibroblasts.The cardiomyocytes diameter of TMZ groups was much smaller than that of the ISO group.Masson color results showed that the level of collagen fiber distribution in the myocardial interstitial and hemal wall of TMZ group was lower than that of ISO group.Caspase 3 color results showed that the number of tan dye distribution was significantly lower in TMZ group than that of the ISO group(Color positive results showed obvious practical color distribution).Conclusion Trimetazidine could prevent myocardial injury and its mechanism might be related to raised Klotho level.

Objective: To explore the safety and feasibility of transumbilical single channel laparoscopic cryptorchidopexy with conventional instruments in treatment of cryptorchidism in children. Methods: Thirty-five children with cryptorchidism were treated by transumbilical single channel laparoscopic cryptorchidopexy with conventional instruments from November 2017 to March 2018 at Department of Pediatric Surgery, the First Affiliated Hospital of Guangxi Medical University.Clinical data were recorded including the general information, operation time, intraoperative blood loss, postoperative testicular position and postoperative complications, etc. Results: All 35 patients completed the cryptorchidopexy in one operation.Among those 35 patients, 28 patients had unilateral lesions and 7 patients had bilateral lesions, with a total of 42 sides.Twenty-six patients were of groin type(33 sides), 9 patients abdominal type(9 sides). The average operation time was (40.4±8.2) min for unilateral lesion, and (62.5±15.3) min for bilateral lesions; the average intraoperative blood loss was (3.0±1.8) mL; and the average postoperative hospital stay was (6.4±2.1) h. Testis of 32 patients (40 sides) were fixed in the middle and bottoms of the scrotum, and testis of 2 patients (2 sides) were fixed on the top of the scrotum.One case had poor healing of naval incision, which was recovered in 1 week after dressing change.There were no postoperative complications such as scrotum hematoma, infection of incision, testicular epididymitis, testicular necrosis, testicular retraction or testicular atrophy, etc.No obvious visible scar was found in the abdomen review 2 weeks postoperatively, with the incidence of complications was 2.9%(1/35 cases). Conclusions Transumbilical single channel laparoscopic cryptorchidopexy with conventional instruments in treatment of cryptorchidism is characterized by small trauma, quick recovery, low complication rate and visible scar free result, which is worthy of clinical promotion, but the operation is relatively complex and the correct procedures are required.

Objective To assess the effect of PCI on quality of life in very old CHD patients.Methods One hundred and ninety-six ≥80 years old CHD patients were divided into PCI group (n=92) and drug therapy group (n=104).Their clinical data were retrospectively analyzed.The patients were followed up for 12 months during which non-event survival rate,readmission rate,and incidence of recurrent arrhythmia,cardiogenic death,bleeding events were recorded.Results The incidence of 3-vessel lesions and serum creatinine level were significantly higher in PCI group than in drug therapy group (70.65％ vs 25.00％,82.63±25.35 μmol/L vs 71.09±22.71 μmol/L,P＜0.01).The non-event survival rate was significantly higher while the readmission rate was significantly lower in PCI group than in drug therapy group (66.30％ vs 50.96％,25.00％ vs 44.23％,P＜0.05).No significant difference was found in recurrent arrhythmia and cardiogenic death between the two groups (P＞0.05).Conclusion PCI can effectively improve the quality of life in very old CHD patients.