AIM:To optimize the flocculation-clarification process of Danshen aqueous extract.METHODS:Based on the single factor experiment,the turbidity of the index components danshensu,protecatechnic aldehyde concentration in the Danshen aqueous extract after flocculation were choose among the kinds and amount of flocculant and flocculation temperature.RESULTS:The turbidity of the chitosan-treated supernatant was lower than that of the ZTC1+1Ⅱ-treated ones,and this treatment can mitigate the membrane fouling better.CONCLUSION:Chitosan is superior to ZTC1+1Ⅱ as the flocculant of the Danshen aqueous extract.

Objective To explore the effect of Zhengu Pills ( a kidney-tonifying and blood-activating Chinese medicine) and Guci Fengshi Pills ( a dampness-dispelling and blood-activating Chinese medicine) on bone remodeling of cell model in vitro. Methods MC3T3-E1 and RAW264.7 cells were differentiated into osteoblasts ( OB) and osteoclasts ( OC) after stimulated by different inductive agents respectively, and then OB and OC were co-cultured. Immunocytochemical staining was applied to identify the construction of co-cultured OB and OC system. The serum containing corresponding medicine was prepared after the rats were given intragastric administration of Diacerein, Zhengu Pills and Guci Fengshi Pills. And then the obtained serum was used for treatment of co-cultured cells separately. At the end of experiment, Western blotting and real-time polymerase chain reaction (PCR) tests were carried out. Results The osteoprotegerin (OPG) expression was increased and gene expression of glycoprotein 130 (gp130) was decreased in co-cultured cells after treatment with the serum containing Zhengu Pills and Guci Fengshi Pills ( P<0.05) . Conclusion Zhengu Pills and Guci Fengshi Pills have therapeutic effects on osteoarthritis ( OA) by inhibiting the bone resorption function of OC by the down-regulation of gp130 and by enhancing bone formation and increasing the bone density through recombinant murine’s RANK ligand (RANKL) -RANK-OPG system.

OBJECTIVE To identify the mental state of laryngopharyngeal reflux disease (LPRD) patients by analyzing anxiety scale and depression scale. METHODS The LPRD patients who received treatment in Fuzhou General Hospital were studied. The healthy volunteers were recruited as control group. Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were used to get the individual scores. Mental states of LPRD patients were finally studied through statistical analysis. RESULTS The scores of anxiety and depression in patients with LPRD were significantly higher than those in control group (anxiety score 50.59±7.24 vs 38.76±7.70, depression score 52.90±7.14 vs 40.63±8.17). The incidence of anxiety and depression in patients with LPRD were significantly higher than that in control group (anxiety rate 56.68％ vs 9.80％, depression rate 50.98％ vs 11.76％). Significant association was detected between ref lux symptom index score and anxiety or depression score in patients with LPRD(r =0.786, r =0.736, P ＜0.05). Significant association between LPRD and anxiety and depression were concluded. CONCLUSION The results showed that the patients with LPRD are more prone to be anxiety and depression mental state than healthy control group, and the severity of LPRD symptoms is significantly correlated with the anxiety and depression. This suggests that the patient's mental state should also be emphasized as well as antacids treatment.

BACKGROUNDOsteoarthritis (OA) is the most common form of human polyarthritis. Many genetic factors have been implicated in OA. It was reported that a polymorphism in the gene of interleukin-6 (IL-6) was associated with OA of knee. The aim of this study was to determine whether functional IL-6 promoter -174G/C (rs1800795) polymorphisms confer susceptibility to knee OA.

METHODSA meta-analysis was conducted on the association between the IL-6 polymorphism and knee OA. Electronic search at PubMed, EMBASE, Weipu database, and Wanfang database was conducted to select studies. Case-control studies containing available genotype frequencies of IL-6 -174G/C were chosen, and odds ratio (OR) with 95% confidence interval (CI) was used to assess the strength of this association.

RESULTSA total of seven studies involving 6 464 subjects (knee OA 3 331 and controls 3 133) were considered in this study. The results suggested that the variant genotypes were not associated with knee OA risk in all genetic models (additive model: OR = 1.144, 95% CI 0.934-1.402, P = 0.194; recessive model: OR = 1.113, 95% CI 0.799-1.550, P = 0.526; dominant model: OR = 1.186, 95% CI 0.918-1.531, P = 0.191). A symmetric funnel plot, the Begg's test (P > 0.05), suggested that the data lacked publication bias.

CONCLUSIONSThis meta-analysis does not support the idea that rs1800795 genotype is associated with increased risk of knee OA. However, to draw comprehensive and more reliable conclusions, further prospective studies with larger numbers of participants worldwide are needed to examine the association between rs1800795 polymorphism and knee OA.

Humans ; Interleukin-6 ; genetics ; Osteoarthritis, Knee ; epidemiology ; genetics ; Polymorphism, Genetic ; genetics ; Promoter Regions, Genetic ; genetics