Purpose To investigate the influence of interpersonal relationship sensitivity on anxiety and depression emotion and subjective well-being for young adults epileptic patients.Methods 87 young adults epileptic patients were divided into interpersonal relationship sensitive group ( study group) and normal group ( control group),according to sensitivity factor ≥ 3 in the symptom checklist(SCL-90).Self-rating anxiety scale ( SAS),self-rating depression scale (SDS) and general well-being scale (GWB) were then established and analyzed.Results The scores on interpersonal relationship sensitivity factor of the study group and control group were 3.64 ±0.51 and 2.73 ± 0.43,respectively,which had remarkable difference ( t =8.91,P ＜ 0.01 ).There were 47 patients with interpersonal relationship sensitivity among the 87 patients,accounting for 54.02％.The scores on SDS and SAS of the study group were higher than control group (P＜0.01 ).The scores on GWB of the study group was significantly lower than the control group (P＜0.01).The scores on GWB of the study group,reflecting the factors about health,energy,satisfaction and interest to life,depression and happy emotion,and relax and nerves,were lower than control group (P＜0.01) ; while the scores on emotion and behavior control were higher than the control group with minor difference (P ＞ 0.05 ).The scores on interpersonal relationship sensitivity factor and SDS,SAS and GWB were positively correlated except for O factor of GWB.Conclusion The level of interpersonal relationship sensitivity has important influence on anxiety and depression emotion and subjective well-being in young adults epileptic patients.

Objective To study the clinical applied value of computed tomography perfusion imaging(CTPI) in early stage of ischemic stroke.Methods Routine CT scan and CTPI were performed in 45 patients with early stage of ischemic stroke(onset within 24 hours).CTPI yields a set of perfusion related parameters of relatine cerebral blood flow(rCBF), cerebral blood volume(rCBV),mean transmit time(rMTT),time to peak(rTTP) and positive enhancement integral (rPE).Results In all 45 patients,21 cases showed concerning lesions in the 29 cases with cerebral ischemia results of routine CT scan.16 cases presented normal results. 24 cases were not showed concerning lesions.The sensitivity of diagnose for early stage of ischemic stroke by CT was 46.67% (21/45).The specificity was 72.41% (21/29).44 cases of CTPI with abnormal perfusion changes were all showed concerning lesions. Only one case of CTPI presented negative result,which was acute brainstem infarction confirmed by follow-up magnetic resonance imaging (MRI).The sensitivity of diagnose for early stage of ischemic stroke by CTPI was 97.78% (44/45).The specificity was 100% (44/44).There was significant difference between CTPI and routine CT scan(P

Objective To investigate the relationship between the numbers of deep medullary veins (DMV) beside the ipsilateral lateral ventricle and the stenotic degree of large artery of ipsilateral internal carotid artery (ICA) system and cerebrovascular reactivity (CVR) in patients with acute cerebral infarction showed on susceptibility-weighted imaging (SWI) in middle cerebral artery (MCA) territory.Methods The patients with acute cerebral infarction in MCA territory who underwent SWI,cerebral angiography and the transcranlal Doppler carbon dioxide challenge test during the hospital stay were enrolled retrospectively.According to the numbers of DMV beside the lateral ventricle on SWI,they were classified into 3 grades:Grade 1:DMV ＜5; Grade 2:DMV 5-10; Grade 3:DMV ＞ 10.The correlations of the DMV grades and the degree of ipsilateral ICA/MCA and CVR were analyzed.Results A total of 44 patients with acute cerebral infarction in MCA territory were enrolled.Twenty-four patients were DMV 1,15 were DMV2,and 5 were DMV 3.The mean stenosis degree of the main arteries of the ipsilateral ICA system was 40.52％ ±30.62％.The mean CVR of MCA was 0.41 ± 0.16.The stenosis degrees in the DMA grade 1,2,and 3groups were 31.31％ ± 26.02％,44.93％ ± 31.03％,and 71.76％ ± 26.68％,respectively.There were significant differences among the 3 groups (F=4.589,P =0.018),and the stenosis degree in the DMV grade 3 group was significantly higher than that in the DMV grade 1 group (P=0.014).The CVRs in the grade 1,2,and 3 groups were 0.45 ± 0.12,0.41 ± 0.18,and 0.22 ± 0.12,respectively.There were significant differences among the 3 groups (F=6.179,P=0.005),and the CVR in the DMV grade 3 group was significantly lower than that in the DMV grade 1 group (P =0.006) and in the DMV grade 2 group (P =0.038).Spearman correlation analysis showed that DMV grade was significantly positively correlated with the stenosis degree (rs =0.381,P =0.013),and was significantly negatively correlated with CVR (rs =-0.375,P=0.012).Conclusions The DMV grade in patients with cerebral infarction was positively correlated with the stenosis degree of responsible large artery and was negatively correlated with the MCA CVR in the responsible side.

Marine medicine is an important part of Chinese medicine and a growth point of marine economy development.Exploration on the exact weight ratios and curative effect to expand marine medicine clinical application is a significant issue in marine medicine research at this stage. This paper presented a research supposition of“documents retrieval, extraction of marine medicine cases, establishment of case database, statistic analysis, data mining, herb pair Game Theory model establishment, herb pair usage under fixed dosage and matching, in order to explore a new idea of researching marine medicine combined with Game Theory. Through the literature retrieval and text extraction, 458 cases with the herb pair ofLong-Gu (LG) andMu-Li(ML) were received to establish the partnership game model. The iterative removals of strictly dominated strategies were adopted. Assuming that LG and ML used with an equal amount of 30 g was the best response, which reached the Nash equilibrium. The results suggested that dosages of LG and ML used equally at the amount of 30 g, 25 g, 24 g, 20 g, 18 g, 15 g were rationalizable strategies. Frequencies of using an equal amount of 30 g LG and ML was the most of mathematical statistics. LG and ML of 30 g were used equivalently to treat symptoms such as insomnia, inappetence, chills, tinnitus, physically and mentally fatigued, spontaneous sweating and night sweating, and so on with the effect of tranquilizing and constricting. The results can be validated and complemented among association rules analysis, mathematical statistics and game model. It implied that it was feasible to apply mathematical statistics and data mining in combination with Game Theory to study the marine medicine herb pair.

Objective To study the effect of human ?-globin matrix attachment region(MAR) on transgene expression in stably transfected CHO cells.Methods Expression vector was constructed,which contained the ?-globin MAR in both sides of Chloramphenicol acetyltransferase(CAT) reporter gene expression cassette in cis,then transfected into CHO cells.The CAT reporter gene expression was analyzed by ELISA method.Results The ?-globin MAR enhanced the CAT gene expression 5.5-fold in stably transformed CHO cells,while the transgene expression variation among individuals of transformants was decreased.Conclusion MAR increase transgene expression in stably transfected CHO cells.

This study was aimed to analyze the research status of marine medicine in China. The research hot issues and feature rules were summarized in order to discuss development trends for the clinic application and further development of marine medicine in recent 20 years. The CNKI library was used as a source to retrieve literatures on marine medicine. The bibliometric methods and Cytoscape 3.1.1 were used to analyze. The results showed that 582 papers were included. A total of 260 papers were published during 2010 to 2014. And 52 papers were published annually. 266 papers in this field were funded. The papers were mostly published in theChinese Journal of Marine Drugs. A total of 278 research institutes were engaged in the research. And the institute published the most paper was the Ocean University of China. There were 12 researchers who had published more than 5 articles. This study focused on the keywords with highly frequency, which were marine drugs, chemical composition, marine natural products, fingerprints and antitumor. It was concluded that marine medicine had become hot issues. The core of the group has been formed. However, most researches had focused on natural products and pharmacological aspects. Traditional Chinese medicine (TCM) theory had not been taken seriously and the infrastructure was weak, which obstructed the clinical application of marine medicine. We should expand the discovery and study of knowledge on ancient herbal literature as well as clinic application of marine medicine in order to build the knowledge database of marine medicine and experience mode of clinic application.

Objective To explore the value of SUV in patients with ascites of unknown cause.Methods A total of 55 patients (24 males,31 females,average age 54.8 years,age range:23-82 years) with ascites of unknown cause who underwent 18F-FDG PET/CT scan were retrospectively enrolled in this study.SUVmax and SUVmean of ascites and liver were measured and their ratios (T/NT) were calculated and compared by two-sample t test.All patients were followed up and had final diagnoses.Sensitivity,specifici ty,accuracy,positive predictive value and negative predictive value were calculated respectively.The diagnostic efficiency was compared among 18F-FDG PET/CT tumor localization alone,ascites radioactivity and ascites cytology examination by x2 test or Fisher exact test.Results Besides the high metabolized primary lesions and/or metastasis,the liver and spleen swamped by ascites with high radioactivity were visualized on MIP images.The SUVmax and SUVmean of malignant ascites were 1.78±0.65 and 1.37±0.38 respectively,which were higher than those of benign ascites (1.11±0.36 and 0.72±0.22; t=4.13,6.82,both P＜0.05).T/NT of malignant ascites was higher than that of benign ascites (SUVmax:0.64±0.20 vs 0.48±0.12,t =3.27,SUVmeax:0.68±0.17 vs 0.38±0.10,t =7.21 ; both P＜0.05).The diagnostic sensitivity,specificity and accuracy of SUV in ascites were 75.0％ (27/36),94.7％ (18/19) and 81.8％ (45/55),respectively.The sensitivity and accuracy of SUV were higher than those of cytological examination (44.4％ (16/36) and 63.6％ (35/55) ; x2 =6.98,4.58,both P＜0.05).The specificity of SUV was higher than that of tumor localization by 18F-FDG PET/CT (63.2％,12/19; x2 =5.70,P＜0.05).Conclusion Significantly higher SUVmax and SUV in malignant ascites than benign ascites were noted,which might play an adjuvant role in patients with ascites of unknown cause.

Objective To investigate the effects of octanol,a gap junction blocker,on the levels of pro-inflammatory cytokines after cerebral ischemia-reperfusion in rats.Methods Seventy-two male SD rats were randomly divided into sham operation group,saline control group,vehicle group,and octanol intervention group (n =18 in each group).The model of transient middle cerebral artery occlusion was induced by the modified suture method.The octanol intervention group was intraperitoneally injected with octanol solution at 5 mmol/kg body weight 30 min before ischemia.The saline control group and the vehicle group were intraperitoneally injected with the same amount of physiological saline and 5％ dimethyl sulfoxide solution 30 min before procedure.The neurological deficit score,brain water content,and cerebral infarction volume in each group were detected after ischemia for 2 h and reperfusion for 24 h.Enzyme-linked immunosorbent assay was used to detect the serum interleukin (IL)-1β,IL-6,and tumor necrosis factor-α (TNF-α) levels.Results Compared with the saline control group and the vehicle group,the neurological deficit score of the octanol intervention group was significantly lower (all P ＜0.05),the brain tissue water content was significantly decreased (P ＜ 0.05),the cerebral infarction volume was significantly reduced (P ＜0.05),and the expression levels of IL-1β,IL-6,and TNF-α were significantly decreased (all P ＜0.05).There were no significant differences in neurological deficit score,brain water content,cerebral infarction volume and serum IL-1 [β,IL-6 and TNF-α levels between the saline control group and the vehicle group.Conclusion Gap junction blocker octanol can reduce cerebral ischemic-reperfusion injury.Its mechanism may be related to the alleviation of inflammatory response.

Objective To investigate the safety and efficacy of rescue stenting after failure of endovascular treatment for acute cerebral large artery occlusive infarction, and compare the differences of safety and efficacy between bridged treatment and direct endovascular treatment in acute cerebral large artery occlusive infarction. Methods The clinical data of 60 patients with acute cerebral large artery occlusive infarction who underwent rescue stenting after failure of endovascular treatment in our hospital form March 2015 to March 2018 were retrospectively analyzed; 26 patients underwent bridged treatment+rescue stenting (bridged treatment group), while 34 patients underwent direct endovascular treatment+rescue stenting (direct treatment group). The recanalization degree immediately after the treatment was evaluated by Modified Thrombolysis in Cerebral Infarction (mTICI) scale. National Institutes of Health Stroke Scale (NIHSS) was performed 24 h and 5-7 d after the treatment, and modified Rankin Scale (mRS) was applied 90 d after treatment to evaluate the neurological functions. In addition, incidences of intracranial hemorrhage and symptomatic intracranial hemorrhage (SICH) and postoperative mortality within 90 d of treatment were calculated. Results (1) Among the 60 patients, 55 patients (91.7％) had revascularization (mTICI 2b-3) immediately after the rescue stenting. NIHSS scores before rescue stenting and NIHSS scores 24 h after rescue stenting (17.50 [15.00, 24.00) vs. 12.00 [8.25, 19.00]) showed statistically significant differences (P<0.05). Twenty-nine patients (48.3％) obtained satisfactory prognosis 90 d after rescue stenting (mRS scores≤2), 9 patients (15.0％) suffered SICH after rescue stenting, and 9 patients died (15.0％). (2) The immediate revascularization rate (92.3％ vs. 91.2％), NIHSS scores 24 h and 5-7 d after surgery (12.00 [7.75, 18.00] vs. 14.50 [10.00, 22.00] and 8.00 [3.00, 12.50] vs. 10.50 [6.75, 16.75]), good prognosis rate 90 d after treatment (57.7％ vs. 41.2％), postoperative SICH incidence (19.2％ vs. 11.8％), and mortality (11.5％ vs. 17.7％) in the bridged treatment group and direct treatment group were not significantly different (P>0.05). Conclusion Rescue stenting is safe and effective for patients with acute cerebral large artery occlusive infarction, no matter it is by bridged treatment or direct intravascular treatment; and the two methods show no significant differences in safety and efficacy

Objective To investigate the effect of octanol,a gap junction blocker,on mitogen activated protein kinase (MAPK) signal pathway after ischemia reperfusion in rats.Methods Seventy-two male SD rats were randomly divided into sham-operated group,DMSO control group,saline control group and octanol group (n=1 8).The focal cerebral ischemia reperfusion models were established by suture method in the later three groups.Rats were injected 5 mmol/kg octanol solution (5％ octanol soluble in 5％ DMSO solution) into the abdominal cavity of rats in the octanol group 30 min before ischemia reperfusion;rats in the DMSO control group were injected with same amount of 5％ DMSO solution,and those in the sham-operated group and saline control group were injected with same amount of saline.At 24 h after reperfusion,Neurological Function Defect Scale was performed;water content in brain tissues was detected by dry-wet method;cerebral infarction volume percentage was detected by TTC staining;the total protein expressions of extracellular signal-regulated kinase 1/2 (ERK1/2),c-jun amino-terminal kinase (JNK),and p38,and protein expressions ofphosphorylased (p)-ERK1/2,p-JNK,and p-p38 were detected by Western blotting.Results The scores of Neurological Function Defect Scale,water content in brain tissues,and infarction volume percentage of the octanol group (1.583±0.651,78.363％±0.672％,and 24.34％±0.19％) were obviously reduced as compared with those in the DMSO control group (2.344±0.743,80.873％±0.831％,and 32.26％±0.21％) and saline control group (2.351±0.732,80.893％±0.734％,and 32.28％±0.24％),with significant differences (P＜0.05).The protein expressions ofp-ERKl/2,p-JNK,and p-p38 in the octanol group (0.201±0.009,0.211±0.011,and 0.191±0.009) were obviously reduced as compared with those in the DMSO control group (0.389±0.019,0.311±0.022,and 0.309±0.021) and saline control group (0.393±0.021,0.304±0.021,and 0.316±0.025),with significant differences (P＜0.05).Conclusion Octanol can reduce ischemic reperfusion injury,whose mechanism may be related to the regulation of MAPK signal pathway.